ACCURIS INSTRUMENTS Smart-Q 800 Quantitative Fluorometer Instruction Manual

ACCURIS INSTRUMENTS Smart-Q 800 Quantitative Fluorometer Instruction

Manual

This Product is for Research Use Only

Thank you for purchasing the Smart-Q™ 800 Fluorometer. This manual details the instrument’s features, specifications, as well as complete operating instructions; please read it carefully prior to operation. Keep this user manual for later use.

Important:
Please keep the box and packaging material for this instrument. If service is required, the box will be needed to ship the instrument to our Service Department.

Initial Inspection
Please inspect the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately.

BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS
PO Box 709
Edison, NJ 08818
USA
Phone: 908-769-5555
Website: www.benchmarkscientific.com / www.accuris-usa.com
Email: info@benchmarkscientific.com / info@accuris-usa.com

Safety Warning and Guidelines

1. Important information for safe use
Users should have a clear understanding of how to use this instrument before operation, please read this manual carefully prior to operation.

NOTICE: Please review this manual in its entirety before operating the Smart-Q™ 800.
For Research Use Only. This instrument is not a medical device and is not intended to be used for clinical testing.

2. Operation and Maintenance
The operation and maintenance of the instrument should comply with the basic guidelines and warnings below. Incorrect operation or maintenance will have detrimental effects on the life, performance, and safety features of the instrument.

3 . Maintenance

The instrument housing should be cleaned regularly using a soft, damp cloth.
To clean the touchscreen, disconnect the power cable, and clean the touchscreen with a soft cloth lightly moistened with LCD cleansing detergent. Take care not to use excessive force as this can damage the touchscreen. Do not use abrasive cleaning solutions or material to prevent the touchscreen from getting scratched.

To disinfect the instrument, disconnect the power cable and use a soft cloth lightly moistened with 70% ethanol, 70% isopropanol, or 10% bleach (0.6% sodium hypochlorite). Take care so that the cleaning solution does not enter the power inlet, the sample port, or the USB drive ports.

4. Transportation and Storage Requirements

Ambient temperature: 10℃ ~ 40℃
Relative humidity: ≤ 70%
Place in a well-ventilated room, away from corrosive gas.

Chapter 1 Introduction

The Smart-Q™ 800 Fluorometer is used for the precise quantitation of nucleic acids and proteins in microliter volumes via fluorescent detection. Under conditions of low concentrations of molecules, concentration values are linear to fluorescence intensity. Thus, unknown samples can be quantitatively analyzed. Reagent kits with fluorescent dye, buffer, and standards are available from Accuris or your local distributor.

Key Features and Benefits:

• 7-inch color LCD touch screen display, easy to operate
• Measure up to 8 samples at a time with 8-strip tubes (0.2mL)
• Fast and accurate detection of DNA, RNA and proteins (< 6 sec)
• Low sample consumption, only 1-20μL of sample is needed for accurate quantification
• Data storage for up to 10,000 sample records
• Sample data can be printed or exported via USB drive
• Customized sample standard curve
• Reagent calculator to assist with volume determinations
• Auto sleep function for power-saving

Chapter 2 Specifications

1. Required Installation Environment

Environmental Temperature: 10°C～40°C
Relative Humidity: ≤ 70%
Input Voltage: 24 VDC 2A

2. Specifications

Chapter 3 Instrument Overview

This chapter provides an overview of the physical characteristics of the Smart-Q 800. When using the instrument for the first time, please read this chapter carefully to familiarize yourself with all the parts.

Structure 1:

Structure 2:

Chapter 4 Installation

4.1 Initial Check
Each Smart-Q 800 has been factory-inspected prior to shipping. Please refer to the included packing list to ensure that all items are included. Contact Accuris or your local distributor if there are any signs of damage to the packaging upon receipt.

Carefully check the instrument’s appearance and housing for cracks, damage, or deformation.

4.2 Installation Conditions
Install the instrument on a clean, dry, and level surface in a well-ventilated room away from corrosive agents

Installation Steps

1. Carefully remove the instrument from its packaging and place it onto a stable worktable. Remove all plastic and tape.
2. Unpack the 24V 2A power adapter and plug it into the socket on the back of the instrument. Then connect another side to an AC100~240V power outlet.
3. Turn on the power. The instrument will start to self-check after powering on.

4.3 Operating Conditions
The Smart-Q uses an 8-strip (0.2ml) fluorescent PCR tube to test samples.
Sample test steps:

1. Insert the 0.2mL tube with sample or standards into sample holder.
2. Close the lid; Click the measurement button on the interface to perform a measurement.

Chapter 5 Software Introduction

5.1 Instrument Startup
Power the instrument on. Upon start-up, the instrument will perform a self- check (Fig. 5.1.01). After the self-check, the Login Interface will be displayed (Fig. 5.1.02).

5.2 Main Menu Interface
After entering the user-specific password (Admin password is set by default to “0000”), the Main Menu Interface will be displayed (Fig. 5.2.01).

Table 5-1 Main Menu Interface Operations

5.3 dsDNA Interface
Note: As the interface of dsDNA, RNA, Protein, Oligo are identical, Only the dsDNA Interface is described in detail in this manual.

5.3.1 dsDNA Interface
Select the test type and click “OK” to enter next interface.
The following takes dsDNA: High Sensitivity as an example.
The specific functions are as follows:

5.3.2 Calibration Curve Interface
Prior to sample measurements, a calibration curve must be created using the standards that are typically included with a quantification kit. Only 2 standards are required to form the calibration curve.

5.3.4 Molarity Calculator

5.1.1 Normalization Calculator
Select the Normalization tab to enter the Normalization calculator interface (Fig. 5.3.10). In this interface, choose the type of Normalization (for example: molarity, concentration, and/or weight), and set the final sample molarity and volume. Click “Calculate” to enter the results interface (Fig. 5.3.11).

The Filling List tab shows the required sample and buffer volume to be added to achieve the desired final sample concentration within the final sample volume. On the Conc. Ratio interface, if a secondary dilution is required, the dilution ratio and the concentration after dilution will be displayed (Fig. 5.3.12). The Initial Conc. interface shows the concentration of the original sample (Fig. 5.3.13). Click “Export” to export current data to an inserted USB drive.

5.2 Fluorescence Interface

5.4 Standard Curve Interface
From the Main Menu Interface, click to enter the Standard Curve Interface (Fig. 5.6.01).

Note: At least 5 standards are required to create the standard curve. For each standard, the average RFU value taken from 3 readings is used for curve generation.

5.4.2 Curve Management

5.5 Reports Interface

Click on a report to view its saved data (Fig. 5.7.03). Click on the sample from the row to view the detailed sample report (Fig. 5.7.04).

5.6 User Management Interface

5.7 Settings Interface

5.7.1 System Settings Interface

Date and Time: Set the system date and time.
Screen Brightness: Adjust the screen brightness.
Sleep Mode: Turn the automatic sleep mode on or off and adjust time required to initiate Sleep Mode.

5.7.2 Upgrade Interface

5.7.3 Maintenance Interface

Chapter 6 Troubleshooting

Quantification Kits and Detection Tubes

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References

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