ACCURIS INSTRUMENTS IsoPure 96 Nucleic Acid Purification System User Manual

ACCURIS INSTRUMENTS IsoPure 96 Nucleic Acid Purification System

Thank you for purchasing our IsoPure 96 Nucleic Acid Purification System. This user manual describes the instrument’s features, specifications, as well as complete operating instructions, please read carefully before operation. Keep this user manual for later use.

Initial Inspection
Please check the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately.

Safety Warnings and Guidelines

Important information for safe use
Users should have a clear understanding of how to use this instrument before operation, please read this manual carefully prior to operation. Any improper operation may cause injured or electric shock. Please read the manual carefully and operate safely according to the guidelines.

Security
The operation and maintenance and of the instrument should comply with the basic guidelines and warnings below. Incorrect operation or maintenance will have effect on using life, performance, and safety features of the instrument. The instrument is normal indoor instrument which conforms to classⅠof GB 4793.1 standard. Please read this manual carefully before operation. The device must be used by experienced personnel with appropriate training.

• The operator should not repair the instrument in case any injury or out-of warranty. If service required, please contact Benchmark Scientific / Accuris Instruments or your local distributor for repair.
• Before powering on, please make sure the voltage of the power supply is consistent with the required voltage. And make sure the rated load of the power outlet is not less than required by the instrument.
• If the power cord is damaged, replace it with the same type and specification power cord. Do not cover anything on the instruments when using. Insert and pull the power line with hand gently and make sure the plug completely insert to the jack.
• The temperature of the heating block is high, please do not touch it during the operation in case any injury.
• The instrument should be kept in an area with minimal dust, away from wet areas and direct sunlight. In additional the installation location should have sufficient ventilation, but away from electromagnetic interference and heat sources. The vent on this instrument are designed for ventilation. Do not cover them in case overheat. When many instruments are used at the same time, the distance between each instrument should be more than 100cm.
• Power off when not in use. If the instrument will not be used for a long period of time, cover it with a cloth or plastic to protect it from dust.
• Disconnect the power cord from the jack at once in the following cases, and contact your local distributor or Benchmark
• Scientific / Accuris Instruments:
  • Liquid enters into the Instrument;
  • Instrument was rained or watered.
  • Abnormal operation: such as abnormal sound or smell.
  • Instrument dropping or outer shell damaged.
  • The function has obviously changed.

Maintenance
The instrument should be cleaned regularly using a soft cloth damp with small amount of alcohol. If any stain on the surface of the instrument, wipe it with soft cloth damp with cleansing cream.

Transportation and storage

• requirements Ambient temperature: 10℃ ~ 35℃ Relative humidity: ≤70%
• Atmosphere pressure range: 500 ~ 1060hpa
• Locate it in a well-ventilated room, away from corrosive gas.

Introduction

The IsoPure 96 nucleic acid purification instrument is a newly launched automatic extraction and purification system for DNA/RNA, proteins and cells. It can absorb, transfer and release magnetic beads by magnetic rod and magnetic rod sleeve to separate magnetic beads and samples. The operation is automatic, fast and simple. Users can extract 1~24, 1~48 or 1~96 samples simultaneously with special kits. Auto pure series can extract samples of animal/plant tissue, blood and body fluids, etc with different kinds of magnetic bead nucleic acid extraction reagents. It is mainly used for the extraction and purification of nucleic acid from human body samples.

1. Application
   This instrument is suitable for the extraction and purification of nucleic acids in animal and plant tissues, blood and body fluids and other samples(mainly used in human body samples).

2. Contraindication
   No contraindication.

3. Service Life
   Service life of the instrument is five years. For production date, please see the label on back of the instrument.

Specifications

1. Working Conditions
   • Environmental Temperature: 10 C～35 C
   • Relative Humidity: ≤70%
   • Input Voltage: AC 100～240V，50Hz/60Hz
2. Basic Parameters
   Model Parameters| ****

IsoPure Mini

|  | ****

IsoPure 96

---|---|---|---
Principle| Magnetic Particle Method, Magnet type
Sample Volume| 200μL-10000μL|  | 50μL—1000μL
Throughput| 24|  | 96
Stability| CV≤5%
Extraction time| 10 ~ 60min/time
Temperature

control module

|

Room temperature to 120℃ for lysis and elution

Temp. Accuracy| ±1℃
Vibrateand mix| 10 different speeds for option
Operation| 7 inch color touch screen, mouse can be connected

Programs

| 8 groups of programs can be preset, and can store 100 groups of programs
Program

management

|

Including create, edit, delete and protocol mode

Extension

interface

|

With USB port and Ethernet port

Network| Extended Ethernet remote control, WiFi function.
Power Supply| AC100-240V, 50Hz/60Hz, 250VA
3. Overall Dimensions

Basic Operating Instructions

This chapter mainly introduces structures, basic operation keys, displays, as well as preparations before starting up. Please read this chapter carefully before using this instrument.

Structures

Front

Back

Cabin Door
The cabin door of IsoPure 96 can be opened which is convenient for cleaning and maintenance.

Transparent Cover
The transparent cover is on the right side of the instrument which is for placing or taking out kits. The cover can be removed which makes it convenient matching with automatic liquid transfer wrokstation.

Touch Screen

• Display screen : Touch screen, mouse also can be connected for operation.
• TAB : Select shortcut program.
• RUN : Start the shortcut program and run the instrument.
• STOP : Stop the operation.

Operations

1. Power Connection
   AC 100 ~ 240V

2. Kits Installation
   Open the cabin door, put kits on the plate position of the rotary table, press position button to turn the rotary table and place all the kits in turn. The IsoPure 96 is suitable for 96-well kits.

3. Detailed Operations

4. Start-up Interface
   Turn on the instrument and make sure the door is closed before start, start-up interface will comes up.
   Then, it will enter into “Run Prog.” interface.

5. Run Program Interface
   This interface including two modes: “shortcut” mode and “list mode”, as shown in below Fig 9 and Fig 10.

In the “List mode” interface, if one program selected/activated in “Shortcut” column, the icon of the program can be displayed on shortcut interface. 8pcs of programs can be activated in maximum at the same time. “SN”, “Name”, “Modify time” and “Lock”are non-editable options.

Run Interface
In “List mode” or “Shortcut” mode, select required program and click “Run” to enter into run interface. When running the program, the instrument will first detect the presence of the kit on the rotary table. If no kit is found on the board of the setup program, the program will prompt to confirm whether the following steps can be continued, as shown in the figure below.

The instrument will install magnetic rod sleeve automatically. If rod sleeves are already installed on the current magnetic rod sleeve rack, “Sleeve loaded, continue?” will pop up. If no magnetic rod sleeve is detected after installing the magnetic rod sleeve, “No sleeve, continue?” will appear.

After the magnetic rod sleeve is successfully installed, the instrument automatically performs the following steps, please see Fig 15.

In the running interface, users can stop, pause, continue or run the program again. The plate with dark blue color, number 1, is the working plate, the red corner marks on it means the plate is running or already finished running, while blue corner mark means the plate which is ready to run, one corner mark means one running and two means two runs. A corner mark represents the plate position used once in the whole program. After the completion of the operation, the No. 8 plate position will be automatically pushed to the transparent cover on the right side.

View
In the list mode or shortcut mode, select the required program, and click “View” button to enter the view interface (See Fig 16). Users can view each parameter settings of the program.

Users can click button in the upper right corner to switch to the graphic display. Highlight displays the plate position which corresponding to the selected step, please see Fig 17 as below.

Then click button to magnetic parameter absorption interface which displays magnetic parameters of selected step as shown in Fig 18.

• “Steps Run”: run the program starts from currently selected step.
• “Option”: view settings of the program, please see Fig 19.

Manage Program
Users can manage all programs in “Manage Prog.” interface.

Management Interface
Management interface is similar to list interface in program operation, except that locking column is non-operable option in program run interface while it’s an operable option in management interface. Click the lock icon to switch lock and unlock. Programs cannot be edited, saved or deleted if in lock state, please make the change in unlock state.

New/Edit interface
When the users click the “New” or “Edit” button, interface of Fig 23 will appear, the main difference between “New” interface and “Edit” interface is whether the program name exists or not, other operations are similar. This interface mainly includes five buttons: “Insert”, “Delete”, “Option”, “Save” and “Back”.

• Insert : click “Insert” to add a new program with default parameters next to the current selected program, the new program should be with a valid name.
• Delete : delete the selected program.
• Option : Option is the high-level parameter setting which applies to the entire program scope.
• Save : save the program file, please note a valid program name is necessary.

“Insert” interface as Fig 22.

• Plate : select a plate position for the coming operation
• Name : set a name of the step
• Mix time : the mixing time for selected plate.
• Mix amp : mix amplitude, the range is from 1 to 100%.
• Wait time : interval time between two steps.
• Volume : The volume is automatically converted to the amplitude of mixing according to the formula.
• Mix speed : 10 kinds of mix speeds from 1 to 10. The higher the value is, the faster the mixing speed will be.
• Temp.: The temperature can be set according to actual requirements, only No.2 and 8 wells can be set.
• Click “ ” to enter parameter settings of magnetic absorption, see below picture please.

• Segments : setting range is 0 ~ 5, it can stop to do magnetic absorption for each segment, magnetization function will be closed if set it to 0.
• Cycle times : repeat magnetic absorption times.
• Mag.speed: It’s magnetic absorption speed when magnetic rod moves under the liquid level. 1 is the slowest while 10 the fastest.
• Lip-lvl: the standing time when magnetic rods closing to liquid level after finishing magnetic absorption which is for magnetic beads gathering in case beads falling off due to liquid surface tension.
• Anti-splash : the standing time when magnetic rods pulling away from liquid level after finishing magnetic absorption, in case cross contamination which caused by liquid splashing due to some special sample tissues falling off.
• 1-5 Segment time : independent magnetic absorption time of each segment, the maximum time can reach to 999 seconds.
• Estimated : The estimated magnetic absorption time of the software.It can only be displayed on the next entry after exiting the interface.

Option
In program new or edit interface, click the “Option” to enter the option interface. The parameters in the option are applied to the whole program as shown in the figure below.

• Confirm : Save all settings and exit.
• Back : Not save all settings and exit.
• Heating Setup : It is used to set the heating type.
  • Heating synchronization : It indicates that the heating and magnetic rod sleeve action are synchronous.
  • Preheating : It indicates that the heating board will rise to the set temperature first, and then the magnetic rod sleeve frame starts to work.
  • Start when : It indicates that the magnetic rod sleeve frame starts to work when the temperature rised to the set temperature which is lower than the target temperature.
• Cooling Setup : It is used to set the cooling type. 3.3.4. Save As/Delete In the “Manage prog” interface, click the save as button to save the file, and click the delete button to delete the file.

System Settings
In system setting interface, “Instrument”, “Date&time”, “Language”, “Air ejector fan”, “Im.&export” and “Upgrade” can be modified.

System Time
Click “Date & time” button to enter modification interface, as shown in the figure below.

The date and time can be adjusted by “+” or “-” buttons.

Language Settings
Two options: Chinese and English.

Select the language , press “Ok “ to save the modification.

Fan
Click “Air Ejector Fan” to choose “On” or “Off”.

Import and Export
Click the “Im.&export” to below interface.

Press the “Import ” to enter U disk directory and then select the program needed, press the “Ok” to import. Press the “export” button to enter the system directory, select programs and then “Ok” to export files to the U disk.

Software Upgrade
Click “Upgrade” to upgrade interface, see Fig 30 please.

Insert the U disk with the latest software in, and then upgrade the interface software or control software of the instrument.

Operation Record
Each run of the program automatically generates a running record.

Users can trace records by “Search” button, see Fig 32 please.

Log exports can be done through the export key.

Lighting
At the rith bottom of the screen, if icon “ ” appears, it means the lighting is on while lighting is off if the icon displays “ ”. Users can click the icon to switch between on and off.

UV Sterilization
The UV disinfection interface is mainly used for the opening and closing of the UV lamp. The time can be set by pressing “+” or “ -” button. The program can automatically determine half of the set time to sterilize the half circle of the rotary table, with a minimum of 2min, as shown in the figure below.

Help
Help interface displays help information and version as shown in the figure below.

Trouble Shooting

1

|

No display after switch on

| Power not connected| Check power
Switch failure| Replace switch
Fuse failure| Replace fuse (5X20 250V 8A)
Others| Contact with Distributor
2| No UV light| UV light failure| Replace light tube Contact with distributor
3| No light| Light failure| Replace light tube Contact with distributor

4

| Can not stop automatically after

opening the door.

|

Sensor failure

|

Contact with distributor

5

| Big variance between actual and display

temperature

|

Sensor failure

|

Contact with distributor

6

|

No heating for       heating strip

|

Sensor failure

|

Contact with distributor

Heater failure

7

|

Instrument can’t run

| Controller failure|

Contact with distributor

Motor failure

8

|

Abnormal sound     during working

| Guide rail installed incorrect|

Contact with distributor

Motor failure
Synchronous belt abrasion
9| Press button not working| Press button failure| Contact with distributor

Software Error Alarm List

Temperature (code: 0)

| T1 Overheat| E011
T1 Open circuit| E015
T1 Short circuit| E016

Electric machinery stroke position (code:4)

| Baffle motor sensor| E404
Rotary motor sensor damaged| E405
Lifting platform motor sensor damaged| E406
Push rod motor sensor damaged| E407
Motor position sensor of magnetic rod sleeve

damaged

| E425
Magnetic rod motor position sensor damaged| E415

LCD, Crystal oscillator, Storage

(code: 7)

| The clock crystal fault| E702
Memory chip E2P damaged Setting parameters lost|

E703

New instrument, instrument type hasn’t been set

Zero has not been calibrated, the instrument zero calibration is not in the 3 well will lead to the

program does not working

Communication (code: 8)

| Moving parts online failure| E801
Rotary parts online failure| E802

Accessory

Abbreviations and Tags

Abbreviations
The following Abbreviations are for reference and will appear in this operation manual.

References

• USA Location information - USA.com
• Home - Accuris Instruments

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