euromex BS.1152-EPLPH bScope Phase Contrast Microscope Series User Manual
- June 5, 2024
- euromex
Table of Contents
euromex BS.1152-EPLPH bScope Phase Contrast Microscope Series
Installation of the fluorescence illuminator and microscope head
Most parts are already mounted on the microscope. Only two parts are packed
separately. The microscope head (B) and the fluorescence illuminator
(F)
- A. Eyepieces
- B. Microscope head
- C. Fluorescence attachment
- D. Turret for fluorescence filters
- E. Protection screen
- F. Fluorescence illuminator
- G. Turret for LEDs
Installation Steps
• Take the microscope with fluorescence attachment, fluorescence illuminator
and head from its packaging
• Place the head on the fluorescence attachment and fasten the screw (on the
right side of the microscope} with a hexagon wrench
• Install the eyepieces into the eyepiece tubes if these are not already
installed (fig 1, A}
• Mount the fluorescence illuminator to the back of the fluorescence
attachment and fasten the screw with a hexagon wrench
• Plug the power cord into the power supply, but don’t turn it on yet
Operation of reflected illumination system (LED fluorescence unit)
- To get the best result when observing with fluorescence please pay attention to the following:
- Select the fluorescence filter that matches the fluorochrome and fluorescent characteristic
- No autofluorescence specimen or device should be in the optical system, such as the cedar immersion liquid wich has cyan autofluorescence
- Always turn off the transmitted illumination before turning on the reflected illumination system
Set and adjust the fluorescence unit
The most important part of the unit is the fluorescence filters assembly.
There are four fluorescence filters, Blue, Green, Violet and Ultraviolet,
mounted in the turret (fig. 1, D} and are indicated as B, G, V and UV on the
turret. There is also a brightfield option and it is indicated as O on the
turret The fluorescence filters in the turret correspond with the LEDs in the
fluorescence illuminator (see table}. There is a turret (fig. 1, G) on top of
the fluorescence illuminator and has the same indication of B, G, V and UV as
the turret for the fluorescence filters When selecting the filter, turn the
turret (D) to the correct filter and choose the corresponding LED by turning
the turret on the illuminator(G}
Operation of the field diaphragm
The field diaphragm has been pre-centered and is ready to use, but due to possible vibration or inclination during transport, it may deviate, therefore always double check If it is not pre-centered, then please follow the following steps:
- Use objective 10x
- Push in the field diaphragm switch (the one in the front, (fig.2, A), a light spot will be visible in the field of view
- Adjust the centering if the light spot deviates from the center of the view of view (fig. 3, a) by using the adjustment screws behind the filed diaphragm switch (fig. 2, B). The light spot should be centered as shown in fig.3,b
- Pull out the switch so that the image fills the field of view as shown in fig. 3, c
