STARLAB S4026-1500 RepeatOne Dispenser User Manual

STARLAB S4026-1500 RepeatOne Dispenser

General

RepeatOne® is a hand-held device for repetitive pipetting. Up to 48 pipetting of the same sample can be made from a single filling, resulting in a considerable reduction in time and expense. RepeatOne® allows the selection of five separate volumes from each TipOne® Repeat Dispenser Tip. Eight different TipOne® Repeat Dispenser Tip sizes provide the most important volumes from 2 to 5000 µl. Use only original STARLAB TipOne® Repeat Dispenser Tips to guarantee the pipette’s performance. Design Principle of RepeatOne

• Volume selection dial
  To determine the pipetting volume, the dial setting (1–5) is multiplied by the minimum pipetting volume of the TipOne® Repeat Dispenser Tip.

• Pipetting lever
  The volume is dispensed by pressing the pipetting lever down until it stops.

• Filling lever
  The TipOne® Repeat Dispenser Tip is filled by sliding this lever upward.

• Locking clamp
  The locking clamp secures the TipOne® Repeat Dispenser Tip firmly into RepeatOne ®. TipOne® Fig.1 Repeat Dispenser Tip

Volume Selection and Choice of TipOne® Repeat

• For repetitive pipetting of a particular volume:
•  Find the desired volume in the columns on the right.
•  The required dial setting and maximum number of pipetting steps for the volume are shown at the top respectively buttom of the columns.
•  Select the corresponding TipOne® Repeat Dispenser Tip from the column on the left

| Application Guide for TipOne ® Repeat Dispenser Tips
---|---
TipOne® Repeat Volume| Volume selection dial
1| 2| 3| 4| 5
0.1 ml| 2 μl| 4 μl| 6 μl| 8 μl| 10 μl
0.5 ml| 10 μl| 20 μl| 30 μl| 40 μl| 50 μl
1.0 ml| 20 μl| 40 μl| 60 μl| 80 μl| 100 μl
2.5 ml| 50 μl| 100 μl| 150 μl| 200 μl| 250 μl
5.0 ml| 100 μl| 200 μl| 300 μl| 400 μl| 500 μl
10 ml| 200 μl| 400 μl| 600 μl| 800 μl| 1000 μl
25 ml| 500 μl| 1.0 ml| 1.5 ml| 2.0 ml| 2.5 ml
50 ml| 1.0 ml| 2.0 ml| 3.0 ml| 4.0 ml| 5.0 ml
| 48| 23| 15| 11| 8
Pipetting Steps

TipOne® Repeat Dispenser Tips
The minimum pipetting volume and the maximum filling capacity is stated on each TipOne® Repeat Dispenser Tips (Fig. 2). The scale facilitates drawing off partial quantities and helps you to estimate the amount of liquid still available for operation. The volume to be pipetted is obtained by multiplying the set number located on the volume selection dial by the minimum pipetting volume of the TipOne® Repeat Dispenser Tips. Eg. 1.0 ml TipOne® Repeat Dispenser Tips, Minimum volume: 1=20 µl at setting 4: 4 x 20 µl = pipetted volume: 80 µl Better precision is achieved by using a TipOne® Repeat Dispenser Tips of small capacity with a high dial setting

Number of Pipetting

Steps

| Position of Volume

Selection Dial

---|---
48| 1
23| 2
15| 3
11| 4
8| 5

25 ml and 50 ml TipOne® Repeat

Dispenser Tips (Fig.3) Attach the adapter to the  25 ml or 50 ml TipOne® Repeat Dispenser Tips by turning it clockwise; re – move by turning it counter clockwise. Operation

Inserting TipOne® Repeat Dispenser Tips

•  Slide the filling lever down until it stops.

•  Raise the locking clamp upward.

•  Insert the TipOne® Repeat tip until it clicks into position. Ensure the TipOne® Repeat’s plunger is fully inserted into the barrel before attaching it to the RepeatOne® (Fig. 4).

•  Ensure the filling lever is completely down, and then lower the locking clamp to secure the TipOne® Repeat tip in place (Fig. 5).
  Filling TipOne®
  Repeat Dispenser Tips Use the following steps forall sizes of tips. 0.1 ml, 0.5 ml, 1.0 ml, and 2.5 ml tips may also be used with a standard 200 µl pipette tip. Attaching a tip facilitates filling from narrow-necked vessels or piercing of sealing foil and also makes dispensing of small volumes more precise.

•  Immerse the tip into the liquid.

•  Fill by slowly sliding the filling lever upward (Fig. 6).

•  Discard the first pipetting step. RepeatOne® is now ready for operation.

Additional Information

• Sliding the filling lever too quickly can cause excessive vacuum. This can cause tiny air bubbles to accumulate in the liquid which may lead to dispensing inaccuracies. Important: The first dispensing step must be discarded in order to release any residual pressure from the dispensing system after filling and to prepare the system for precise dispensing. Small bubbles in the TipOne® Repeat Dispenser Tip be – neath the piston will not affect dispensing accuracy since the residual stroke lock prevents the dispensing of any remaining sample after the last dispense. It is not necessary to completely fill the TipOne® Repeat Dispenser Tip. Partially filling will not cause pipetting inaccuracies. Dispensing with a 5.0 ml or 10 ml TipOne® Repeat Dispenser Tip is normally carried out without attaching a pipette tip. Never attach a standard tip when working with the 25 ml and 50 ml TipOne® Repeat tips. A new TipOne® Repeat Dispenser Tip must be used each time to avoid carry-over and contamination.
  Dispensing

•  Check the volume selection dial.

•  Wipe the tip of the TipOne® RepeatDispenser Tip with lint-free tissue.

•  Hold the dispensing tip so it touches the inner wall of the vessel.

•  Dispense the liquid by completely depressing the dispensing lever.

Removing TipOne® Repeat Dispenser Tips

•  Hold RepeatOne® over a beaker.
•  Slowly depress the filling lever down to the stop. This will completely empty the TipOne® Repeat Dispenser Tip.
•  Raise the locking clamp upward.
•  Remove the TipOne® Repeat Dispenser Tip. Note: When using the 25 ml or 50 ml TipOne® Repeat Dispenser Tips, the reusable adapter is removed by turning it counter clockwise.

Safety Precautions

• When removing TipOne® Repeat Dispenser Tips, please note the following: • Do not touch the tip end, especially when using hazardous reagents!
•  Empty the dispenser tips into a proper discard receptacle to avoid splashing and aerosols.
•  Treat any retained fluid as a bio-hazard. Do not allow thefluid to come into contact with laboratory personnel.
•  If dispensing a liquid with a surface tension significantly different from water, inaccuracies or leaking can occur.
•  The RepeatOne® with an attached, partially filled TipOne® Repeat Dispenser Tip should be stored in a stand to prevent air from entering the dispenser tip

Troubleshooting

the down position;

therefore, locking clamp cannot be completely raised.

| Depress the filling lever downward and raise the locking clamp as far as it will go.
The dispenser tip piston is not completely inserted in the barrel.| Slide the piston into the proper position.
Unable to fully lift the filling lever.| Locking clamp incompletely depressed.| Completely depress the locking clamp.
Excessive air bubbles when filling the TipOne® Repeat Dispenser Tip.| Filling lever lifted slightly before immersing into the liquid.| Draw up some solution and then discard it (using filling lever). Then refill the TipOne® Repeat Dispenser Tip.
Leaking of TipOne® Repeat Dispenser Tip.| Replace the dispenser tip with a new one.
Inconsistent pipetting.| Volume selection dial has not clicked into position.| Click volume selection dial into position.
Dripping TipOne® Repeat Dispenser Tip.| Leaking TipOne® Repeat Dispenser Tip.| Replace with a new dispenser tip.
Attached TipOne® Repeat Dispenser Tip is loose.| Use more force to attach the TipOne® Repeat Dispenser Tip.
RepeatOne® jams or does not function.| | Return the RepeatOne® for repair or replacement.

Maintenance

• Maintenance
  External surfaces of RepeatOne® can be cleaned with warm water or isopropyl alcohol. Apart from the need to clean externally, the RepeatOne® is completely maintenance-free. Important: under no circumstances should the RepeatOne® be dismantled! RepeatOne® and TipOne® Repeat Dispenser Tips are not autoclavable!

• Service
  If a problem cannot be solved with the aid of the Troubleshooting Guide, return RepeatOne® to your regional STARLAB office or distributor for repair or replacement. Important: Please decontaminate the dispenser before return. Proper decontamination has to be confirmed in a separate “declaration of decontamination”. Please contact STARLAB for more detailed information.

• Warranty
  Please check that the unit has been received complete and undamaged. Save all packaging and documents until you have thoroughly inspected your shipment; if you find that your order is incorrect or damaged please call STARLAB for instructions for its return. This warranty is valid for 24 months, but only if the product has been used and cared for according to this User Manual. No liability is accepted for loss or damage arising from incorrect use. The liability is limited to the repair or replacement of the unit, or a refund of the purchase price, at STARLAB’s option. STARLAB is not liable for any consequential damages. We refer to our general terms of sale. STARLAB reserves the right to alter the specifications without prior notice.

Ordering Information

RepeatOne ® Dispenser and Adapter

Cat-No.| Description| Pack Size
S4026-1500| RepeatOne® Dispenser| 1
S4756-2500| 25 ml Adapter| 1
S4756-5000| 50 ml Adapter| 1
S4765-2500| 25 ml Adapter (sterile)| 1
S4765-5000| 50 ml Adapter (sterile)| 1
TipOne ® Repeat Dispenser Tips

Cat-No.| Description| Pack Size
S4751-0010| 0.1 ml TipOne® Repeat Dispenser Tips| 100
S4751-0050| 0.5 ml TipOne® Repeat Dispenser Tips| 100
S4751-0100| 1.0 ml TipOne® Repeat Dispenser Tips| 100
S4751-0250| 2.5 ml TipOne® Repeat Dispenser Tips| 100
S4751-0500| 5.0 ml TipOne® Repeat Dispenser Tips| 100
S4751-1000| 10 ml TipOne® Repeat Dispenser Tips| 100
S4751-2500| 25 ml TipOne® Repeat Dispenser Tips| 100
S4751-5000| 50 ml TipOne® Repeat Dispenser Tips| 100
TipOne ® Repeat Dispenser Tips (sterile)

Cat-No.| Description| Pack Size
S4761-0010| 0.1 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-0050| 0.5 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-0100| 1.0 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-0250| 2.5 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-0500| 5.0 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-1000| 10 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-2500| 25 ml TipOne® Repeat Dispenser Tips (sterile)| 100
S4761-5000| 50 ml TipOne® Repeat Dispenser Tips (sterile)| 100

Please only use the accessories recommended by STARLAB. Using non-recommended disposables can reduce the precision and accu- racy, and life, of RepeatOne®. We do not honour any warranty or accept any responsibility for damage resulting from such action.

Technical Data

TipOne® Repeat Dispenser Tip Size| Dispensing Volume| Systematic Error (Inaccuracy)| Random Error (Imprecision; CV)
---|---|---|---
Minimum| Maximum| Minimum| Maximum| Minimum| Maximum
0.1 ml| 2 μl| 10 μl| ± 1.6 %| ± 1.6 %| £ 5.0 %| £ 2.5 %
0.5 ml| 10 μl| 50 μl| ± 1.0 %| ± 1.0 %| £ 1.5 %| £ 0.8 %
1.0 ml| 20 μl| 100 μl| ± 0.9 %| ± 0.9 %| £ 0.9 %| £ 0.6 %
2.5 ml| 50 μl| 250 μl| ± 0.8 %| ± 0.8 %| £ 0.8 %| £ 0.5 %
5.0 ml| 100 μl| 500 μl| ± 0.6 %| ± 0.6 %| £ 0.6 %| £ 0.3 %
10 ml| 200 μl| 1000 μl| ± 0.5 %| ± 0.5 %| £ 0.6 %| £ 0.3 %
25 ml| 500 μl| 2500 μl| ± 0.4 %| ± 0.4 %| £ 0.6 %| £ 0.25 %
50 ml| 1000 μl| 5000 μl| ± 0.3 %| ± 0.3 %| £ 0.5 %| £ 0.25 %

Test conditions in accordance with EN ISO 8655

• Liquid:                         Water bi-distilled, degassed Test Temperature:               20–25°C, ±0.5 % constant
• Operating Temperature: +4 °C to +40 °C
• Measurement :                     Performed with original TipOne®
• Repeat Dispenser Tips in accor- dance with EN ISO 8655
• Technical specifications are subject to change without notice!

 Quick Reference Guide

**** The Quick Reference Guide should only be used after the operator is thoroughly acquainted with the detailed instructions, otherwise, errors may occur.

•  Look for desired volume in the volume selection chart (See page 5).
•  Select appropriate TipOne® Repeat tip by number of dispensing steps.
•  Set volume selection dial to the corresponding position.
•  Slide filling lever down.
•  Raise locking clamp upward.
•  Insert TipOne® Repeat tip into position.
•  Lower locking clamp and press closed.
•  Immerse the tip of t he TipOne® Repeat tip into liquid.
•  Slide filling lever upward.
•  Discard first dispensing step.
•  Dispense.
•  Empty TipOne® Repeat tip completely.
•  Raise locking clamp upward.
•  Remove TipOne® Repeat tip.

References

• Homepage - STARLAB
• Homepage - STARLAB
• Homepage FR - STARLAB
• Homepage IT - STARLAB
• Starlab Group

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