dynamic heliX Plus Adapter Strand 2 no Fluorophore User Manual

June 4, 2024
Dynamic

dynamic heliX Plus Adapter Strand 2 no Fluorophore

Key Features

  • Adapter strand – no fluorophore for functionalization of heliX® Adapter Chip Spot 2.
  • Compatible with heliX® Adapter Chip.
  • Includes Adapter strands for 50 regenerations.

heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.

Product Description

  • Order Number: AS-2-nf

Table 1. Contents and Storage Information

Material Cap Concentration Amount Buffer Storage
Ligand strand 2 – no fluorophore White 400 nM 5 x 100 µL TE40 [
1 ] -20°C
  • For research use only.
  • This product has a limited shelf life, please see the expiry date on label.
  • To avoid many freeze-thaw cycles please aliquot the nanolever.

In-solution hybridization of adapter and ligand strands

  1. Mix Ligand strand 2 – no fluorophore (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v). Example: The Ligand strand could have a DNA overhang and a fluorophore on top.
  2. Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
  • Solution is ready to use for biochip functionalization.
  • Stability of the solution is related to the stability of the ligand molecule.

Table 2. Useful Products

Material Content Order No
DNA enzyme activity kit Adapter strand 1 – nf already pre-

hybridized to the Template- 32-P-Ra DNA strand for Spot 1

cTemplate – Adapter strand 2 – nf already pre-hybridized to the Template-32-P-Ra DNA strand for Spot 2.

| HK-EA-1

Contact

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany

Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA

Order Information
order@dynamic-biosensors.com

Technical Support
support@dynamic-biosensors.com

www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.

[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
www.dynamic-biosensors.com

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