dynamic heliX Plus Adapter Strand 2 no Fluorophore User Manual
- June 4, 2024
- Dynamic
Table of Contents
dynamic heliX Plus Adapter Strand 2 no Fluorophore
Key Features
- Adapter strand – no fluorophore for functionalization of heliX® Adapter Chip Spot 2.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
- Order Number: AS-2-nf
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Ligand strand 2 – no fluorophore | White | 400 nM | 5 x 100 µL | TE40 [ | |
| 1 ] | -20°C |
- For research use only.
- This product has a limited shelf life, please see the expiry date on label.
- To avoid many freeze-thaw cycles please aliquot the nanolever.
In-solution hybridization of adapter and ligand strands
- Mix Ligand strand 2 – no fluorophore (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v). Example: The Ligand strand could have a DNA overhang and a fluorophore on top.
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Solution is ready to use for biochip functionalization.
- Stability of the solution is related to the stability of the ligand molecule.
Table 2. Useful Products
| Material | Content | Order No |
|---|---|---|
| DNA enzyme activity kit | Adapter strand 1 – nf already pre- |
hybridized to the Template- 32-P-Ra DNA strand for Spot 1
cTemplate – Adapter strand 2 – nf already pre-hybridized to the Template-32-P-Ra DNA strand for Spot 2.
| HK-EA-1
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information
order@dynamic-biosensors.com
Technical Support
support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
www.dynamic-biosensors.com