Oxford Nanopore PRO-INT002 PromethION Sequencing Device User Guide
- June 1, 2024
- Oxford Nanopore
Table of Contents
Oxford Nanopore PRO-INT002 PromethION Sequencing Device User Guide
Pre-installation
This Quick Start Guide contains everything you need to set up your
PromethIONTM 2 Integrated and to check that the device is ready for use.
Before using the device, familiarise yourself with the following:
PromethION 2 Integrated user manual
community.nanoporetech.com/to/p2i
Safety and regulatory information
community.nanoporetech.com/to/safety
For detailed information and troubleshooting, view the user manual.
What’s in the box
What you will need
- The PromethION 2 Integrated ships with 5 x power cables (1 US, 1 UK, 1 EU, 1 CN, 1 AUS) for international use.
Set up your device
- Unpack your PromethION 2 Integrated device*
- Attach the cables and peripherals in the order shown opposite
- Press the power switch
Rear input/output
- The device must be placed away from direct sources of heat or sunlight. Place device on a well-supported, strong, clean bench. Allow 30 cm clearance rear and sides, and do not cover ventilation grilles. See user manual for detailed installation advice.
† Connect only one monitor.
Log in to your PromethION 2 Integrated
On initial setup, we recommend using an external monitor. For subsequent uses, you can use the touchscreen and/or an external monitor.
Monitor:
-
Log in to your PromethION
Username: prom
*Password: prom** -
Open MinKNOWTM Click the wheel icon on the desktop to load MinKNOW.
-
Log into MinKNOW. Use your Oxford Nanopore account details.
- For added security, we recommend changing your password after device setup using the instructions in the user manual.
Update MinKNOW
For the latest sequencing features, update MinKNOW:
You must now shut down your PromethION 2 Integrated using the instructions in step 4. Once complete, press the power switch and repeat step 2.
Power off
Ensure the device is not performing any tasks and remove any CTCs from the
flow cell positions if present.
In MinKNOW, click or press (if using the touchscreen) the top-right power
icon, then Shutdown in the pop-up window.
After repeating step 2, you will be able to complete your software update.
Then, proceed to step 5.
Perform hardware check
A hardware check is required before performing your first PromethION 2 Integrated sequencing run. To run a hardware check, follow the on-screen instructions in MinKNOW, then follow the instructions below. You will require your two PromethION Configuration Test Cells (CTCs).
Hardware check overview:
- Insert the CTCs into the device as shown here and close the lid of the device.
- In the MinKNOW software, the flow cell status indicators (the two boxes) will change colour from grey to white.
- Press Select all available. This will change the colour of the flow cell status indicators (the two boxes) on the MinKNOW Hardware check panel to dark blue.
- Press Start in the bottom right.
- Check the flow cell positions show a to pass the hardware check.
- Remove the CTCs from the flow cell positions after you complete the hardware check.
Note: If your hardware check fails, see Support in the Additional information section.
Discover the Nanopore Community
Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocols.
Tip: Learn how to analyse your nanopore data:nanoporetech.com/analyse
Additional information
store.nanoporetech.com/device-warranty.html
warranty:
community.nanoporetech.com/to/warranty
community.nanoporetech.com/support/returns
store.nanoporetech.com
community.nanoporetech.com/docs
community.nanoporetech.com/support
Technical specification
Oxford Nanopore Technologies
phone +44 (0)845 034 7900
email [email protected]
@nanopore
Oxford Nanopore Technologies, the Wheel icon, MinKNOW and PromethION are
registered trademarks of Oxford Nanopore Technologies plc in various
countries. All other brands and names contained are the property of their
respective owners. © 2024 Oxford Nanopore Technologies plc.
All rights reserved. Oxford Nanopore Technologies products are not intended
for use for health assessment or to diagnose, treat, mitigate, cure, or
prevent any disease or condition.
ONT-08-01121-00-1 | BR_1221(EN)_V1_01Jan2024
References
- community.nanoporetech.com/docs
- nanoporetech-customers - Sign In
- Community
- Community
- Welcome to Oxford Nanopore Technologies
- Oxford Nanopore technologies data analysis solutions
- store.nanoporetech.com
- store.nanoporetech.com/device-warranty.html
- Welcome to Oxford Nanopore Technologies