Oxford Nanopore 002 SEQ-P PromethION 2 Solo User Guide

: June 1, 2024
: Oxford Nanopore

Table of Contents

Oxford Nanopore 002 SEQ-P PromethION 2 Solo
Product Usage Instructions
Pre-installation
PromethION 2 Solo
Installation guidance
Device setup — GridION or laptop
Perform a hardware check
Power off
Discover the Nanopore Community
Additional information
Technical specification
Documents / Resources

Oxford Nanopore 002 SEQ-P PromethION 2 Solo

Oxford-Nanopore-002-SEQ-P-PromethION-2-Solo-product-
image

Specifications:

Product: PromethION 2 Solo
Includes: PromethION 2 Solo device, 2 x Configuration Test Cells (CTCs), USB Type-C cable, Power adapter
Connectivity: USB Type-C

Product Usage Instructions

Installation:
To use the device with a laptop:

Check that your computer meets the IT requirements.
Power on your laptop.
Install the latest MinKNOW software for the device from the Software Downloads page.

To use the device with GridION:

Check that the GridION serial number is GXB002 or above.
Power on your GridION.
Update to the latest GridION software from the Software Downloads page.

Device Setup:

Plug the AC power adapter into the mains supply and connect it to the PromethION 2 Solo power port.
Ensure no CTCs are inserted into the PromethION 2 Solo.
Connect the USB Type-C cable to your laptop or GridION and the other end to the PromethION 2 Solo.
Toggle the power switch on the device.

Performing a Hardware Check:
A hardware check is necessary before starting a sequencing run. Follow these steps:

Load both CTCs into the PromethION 2 Solo Flow Cell positions and close the lid.
Wait for the flow cell positions to initialize with a blue LED.
In MinKNOW software, press “Select all available positions” to start the hardware check.

FAQs:
For more information and troubleshooting, refer to our FAQs at qr.nanoporetech.com/beg1C1

Pre-installation

This Quick start guide contains everything you need to set up your PromethION™ 2 Solo and to check that the device is ready for use.

Before using the device, familiarise yourself with the following:

PromethION 2 Solo user manual
community.nanoporetech.com/to/p2solo
Technical specifications
community.nanoporetech.com/to/techspec
Safety and regulatory information
community.nanoporetech.com/to/safety
IT requirements
community.nanoporetech.com/to/p2solo-it

For detailed information and troubleshooting, view the user manual.

PromethION 2 Solo

$Oxford-Nanopore-002-SEQ-P-PromethION-2-Solo-fig- $1$$

Installation guidance

To use the device with a laptop:

Check that the computer you are using meets the IT requirements (see start of this Quick Start Guide).
Power on your laptop.
Install the latest MinKNOW software for the MinION Mk1B and the PromethION 2 Solo devices from the Software Downloads page (see below). Once the software is installed, click the Nanopore wheel icon that appears on the desktop.

Software downloads
community.nanoporetech.com/downloads

To use the device with GridION:

Check that the GridION serial number on the label is GXB002 or above.
Power on your GridION.
Update to the latest GridION software from the Software Downloads page (see below). Once the software is updated, click the Nanopore wheel icon on the desktop. $Oxford-Nanopore-002-SEQ-P-PromethION-2-Solo-fig- $3$$

Device setup — GridION or laptop

Plug the AC power adapter (country-specific) into the mains supply and plug the other end into the PromethION 2 Solo power port .
Make sure there are no Configuration Test Cells (CTCs) inserted into the PromethION 2 Solo.
Plug the USB Type-C cable into the correct ports on your host laptop or GridION and plug the other end into the PromethION 2 Solo, with the P2 icon on both of the USB Type-C connectors facing upwards.
Toggle the power switch on the PromethION 2 Solo. $Oxford-Nanopore-002-SEQ-P-PromethION-2-Solo-fig- $4$$

FAQs
qr.nanoporetech.com/beg1C1

Perform a hardware check

A hardware check is required before performing your first PromethION 2 Solo sequencing run. To run a hardware check, follow the on-screen instructions in MinKNOW, then follow the instructions below. You will require your CTCs . $Oxford-Nanopore-002-SEQ-P-PromethION-2-Solo-fig- $6$$

Hardware check overview:
Load both CTCs into the PromethION 2 Solo Flow Cell positions as shown below and close the lid of the device.

Wait until the flow cell positions on the device intialise with a blue LED. In the MinKNOW software, the flow cell status indicators (the two boxes) will change colour from grey to white.
Note: The LEDs pulse white and then turn a solid blue when the CTCs are inserted.
Press Select all available positions. This will change the colour of the flow cell status indicators (the two boxes) on the MinKNOW Hardware check panel to dark blue.
Note: The LEDS will pulse green while performing the hardware check.
Press Start in the bottom right of the MinKNOW display.
Check the flow cell positions show a above the flow cell icon to pass the hardware check.
Remove the CTCs from the flow cell positions after you complete the hardware check.

Insert the CTCs into the flow cell positions as shown below. Note: If your hardware check fails, see Support in the Additional information section.

Power off

Power off your PromethION 2 Solo:

Remove the PromethION CTCs from the device.
Toggle the power switch to the off position on the PromethION 2 Solo.

Power off your laptop or GridION:
To power off your GridION using MinKNOW, follow the workflow below: OR
You can close MinKNOW and power off your laptop now.

Discover the Nanopore Community

community.nanoporetech.com

Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocols.
Tip: Learn how to analyse your nanopore data at: nanoporetech.com/analyse

Additional information

Warranty
- A license and warranty can be purchased for your device here: store.nanoporetech.com/device-warranty.html
- Flow cell warranty: nanoporetech.com/warranty
Recycle used flow cells
- Oxford Nanopore is committed to environmental sustainability.
- You can help by sending your flow cells for recycling.
- Find out how: community.nanoporetech.com/support/returns
Place your next order
Buy more consumables at the Oxford Nanopore Store: store.nanoporetech.com
Documentation
Documentation for your device is available on the Nanopore Community: community.nanoporetech.com/docs
Support
For all of your customer and technical support needs, visit: community.nanoporetech.com/support

Technical specification

| PromethION 2 Solo
---|---
Model number| PRO-SEQ002
Supply voltage (V)| Power supply unit input: 100-240 AC ± 10% (50/60Hz) PromethION 2 Solo input: 12 DC
Maximum rated current (A)| 5
Maximum rated power (W)| 60
Size (H x W x D) (mm)| 152 x 110 x 87
Weight (kg)| 1.5
Installation ports| 1 x USB Type-C (3.0 @ 5 Gbps), 1 x 12 VDC Barrel Power connector
Software installed| None
Compute specification| N/A
Environmental conditions|

Functional range of electronics is within environmental temperatures of +5°C to +40°C. Users should allow 30 cm clearance to the rear and sides of the device.
Designed to sequence in environmental temperatures of +18°C to +25°C. Use within 30%–75% relative non-condensing humidity limits.
Intended for indoor use.
Can be used up to altitudes of 2,000 m. The device has a Pollution Degree 2.

Oxford Nanopore Technologies
phone +44 (0)845 034 7900
email support@nanoporetech.com
@nanopore

www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, GridION, MinION, MinKNOW, and PromethION are registered trademarks of Oxford Nanopore Technologies plc in various countries. All other brands and names contained are the property of their respective owners. © 2024 Oxford Nanopore Technologies plc. All rights reserved. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.
ONT-08-00972-00-3 | BR_1156(EN)_V2_01Jan2024