hygiena Allergen RM 800 Hazelnut Detection Kit User Manual
- June 17, 2024
- Hygiena
Table of Contents
hygiena Allergen RM 800 Hazelnut Detection Kit
Specifications
- Product Name: Hazelnut Detection Kit
- Product No.: KIT230059
- Revision: A, December 2023
- PCR kit for the qualitative detection of hazelnut DNA using real-time PCR instruments
- Kit for 64 reactions
- For food testing purposes
- FOR IN VITRO USE ONLY
foodproof®
Hazelnut Detection Kit Ready Reference Guide
Product No. KIT230059
PCR kit for the qualitative detection of hazelnut DNA using real-time PCR
instruments.
Before starting, it is strongly recommended to read the entire product manual
available on our website.
PROGRAM SETUP
Program your real-time PCR instrument before setting up the PCR reactions.
Select the following channels:
FAM (hazelnut) and HEX (Internal Control).
For
some real-time PCR instruments, the probe quencher as well as the use of a
passive reference dye must be specified. This kit contains probes with a non-
fluorescent “dark” quencher and no passive reference dye.
DATA INTERPRETATION
Verify results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results. Always compare samples to positive and negative controls. Review data from each channel and interpret results as described in the table.
PREPARATION OF THE PCR MIX
Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. Thaw reagents, mix (do not vortex!) and briefly spin vials before opening.
-
ADD PCR MIX
Pipet 20 µL of Master Mix into each strip or plate well (n samples + 2 controls). -
ADD SAMPLES AND CONTROLS
Pipet 5 µL of samples, Negative Control (colorless cap) or Control Template (purple cap) into respective wells.
OPTIONAL: STANDARD CURVE
For quantification, prepare the standard curve.
Please refer to Allergen RM 800 (Product No. KIT230009) product manual. -
SEAL
Seal strips/plate accurately.
-
CENTRIFUGE
Briefly spin strips/plate in a suitable centrifuge. -
START REAL-TIME PCR RUN
Cycle samples as described above.
foodproof®
Hazelnut Detection Kit
KIT230059
Kit for 64 reactions Store kit at -15 to -25 °C
For food testing purposes FOR IN VITRO USE ONLY Made in Germany
Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com