Thermo Scientific 7400 CRYO Plus Liquid Nitrogen Storage System Instruction Manual

Thermo Scientific 7400 CRYO Plus Liquid Nitrogen Storage System

Product Information

Specifications

• Models: CryoPlus 1, CryoPlus 2, CryoPlus 3, CryoPlus 4
• LN2 Capacity: 90L, 90L, 200L, 200L, 340L, 340L, 552L, 552L
• Voltage: 100-120V, 200-230V, 100-120V, 200-230V, 100-120V,\ 200-230V, 100-120V, 200-230V

Product Usage Instructions

1. Safety Precautions

Before using the CryoPlus system, it is important to follow these safety precautions:

• Read and understand the instruction manual thoroughly.
• Ensure that only qualified service personnel perform internal adjustments and maintenance.
• Always use personal O2 detection equipment when working with the system in a closed environment.
• Do not store samples that are intended to be reintroduced into the human body.
• Avoid operating the system in potentially explosive environments or storing flammable inventory.

2. Installation

Follow these steps to install the CryoPlus system:

1. Select a suitable location for the system that is well-ventilated and away from potential hazards.
2. Ensure that the voltage supplied to the system matches the specified voltage range.
3. Place the system on a stable and level surface.
4. Connect the system to a power source using the provided power cord.

3. Operation

To operate the CryoPlus system, follow these instructions:

1. Open the lid of the system by unlocking it using the provided tumbler lock.
2. Place the samples or storage containers inside the system, ensuring they are properly labelled and secured.
3. Close the lid securely and lock it using the tumbler lock.
4. Set the desired temperature using the control panel.
5. Monitor the temperature and LN2 level regularly to ensure proper storage conditions.

4. Maintenance

Regular maintenance is essential to ensure optimal performance of the CryoPlus system. Follow these maintenance guidelines:

• Clean the exterior of the system regularly using a mild detergent and a soft cloth.
• Inspect the system for any signs of damage or wear and replace any faulty components.
• Check and refill the LN2 as needed, following the manufacturer’s guidelines.
• Perform any required calibration or adjustments according to the instruction manual.

Frequently Asked Questions

• Q: Can I store samples that will be used in medical procedures in the CryoPlus system?
  • A: No, the CryoPlus system is not intended for storing samples that will be reintroduced into the human body.
• Q: What should I do if I notice any damage or wear on the system?
  • A: If you notice any damage or wear on the system, contact qualified service personnel for inspection and potential component replacement.
• Q: How often should I refill the LN2?
  • A: The frequency of LN2 refills will depend on the usage and storage conditions. Regularly monitor the LN2 level and refill as needed.

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“Models covered in this manual”

MANUAL NO. 7007400

CAUTION
Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge (ESD)

• Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual may result in damage to the unit, injury to operating personnel, and poor equipment performance. up
• Caution All internal adjustments and maintenance must be performed by qualified service personnel. up
• Warning Whenever working with liquid nitrogen storage equipment in a closed environment, the use of personal O2 detection equipment is strongly recommended. up
• Intended Use: The Liquid Nitrogen Storage Systems described in this manual are used for storing samples at cryogenic temperatures (-130°C or below).

The Liquid Nitrogen Storage Systems are intended for use:

• As a cryogenic storage device for research use; storing samples at temperatures between -130°C and -186°C (-202°F to -303°F).
• As a cryogenic storage device for medical applications; storing samples at temperatures between – 130°C and -186°C (-202°F to -303°F).

Non-Intended Use: These Liquid Nitrogen Storage Systems are not intended to store samples that are re-introduced into the human body. These Liquid Nitrogen Storage Systems are not intended to be operated in potentially explosive environments and are not intended for storage of flammable inventory. Material in this manual is for information purposes only. The contents and the product it describes are subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of this manual. 2022 Thermo Fisher Scientific. All rights reserved.

Safety precautions

• This symbol when used alone indicates important operating instructions which reduce the risk of injury or poor performance of the unit.
• WARNING: This symbol indicates potentially hazardous situations which, if not avoided, could result in serious injury or death.
• WARNING: This symbol indicates situations where dangerous voltages exist and potential for electrical shock is present.
• CAUTION: This symbol, in the context of a CAUTION, indicates a potentially hazardous situation which if not avoided could result in minor to moderate injury or damage to the equipment.
• CAUTION: This indicates a situation which may result in property damage.
• Before installing, using or maintaining this product, please be sure to read the manual and product warning labels carefully. Failure to follow these instructions may cause the product to malfunction, which could result in injury or damage.
• The snowflake symbol indicates low temperatures and risk of frost bite. Do not touch bare metal or samples with unprotected body parts.
• This symbol indicates a need to use gloves during indicated procedures. If performing decontamination procedures, use chemically resistant gloves. Use insulated gloves for handling samples.
• Use this product only in the way described in the product literature and in this manual. Before using it, verify that this product is suitable for its intended use. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
• Do not modify system components, especially the controller. Use OEM exact replacement equipment or parts. Before use, confirm that the product has not been altered in any way.
• WARNING: Your unit must be properly grounded in conformity with national and local electrical codes. Do not connect the unit to overloaded power sources.
• WARNING: Disconnect the unit from all power sources before cleaning, troubleshooting, or performing other maintenance on the product or its controls.
• WARNING: This unit is not for storage of flammable materials.
• WARNING: Unauthorized repair of your freezer will invalidate your warranty. Contact Technical Service at 1-800-438-4851 for additional information.
• WARNING: No equipment that uses an open flame should be placed inside the freezer. This will harm the unit, hamper functionality and compromise your safety.
• CAUTION: Do not use any battery powered or externally-powered equipment in the freezer.
• WARNING: Nitrogen is a potential asphyxiant and can cause rapid suffocation without warning. Store and use in area with adequate ventilation. DO NOT vent container in confined spaces. DO NOT enter confined spaces where gas may be present unless area has been  well ventilated. Oxygen monitors are recommended. Refer to “Handling Liquid Nitrogen” in the appendix at the end of this manual.
• Potential biological hazards. Proper protective equipment and procedures must be used.
• Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
• Contains parts and assemblies susceptible to damage by electrostatic discharge.
• Alternating current, Single phase.
• On
• Off
• Protective Conductor Ground Terminal.
• Earth Ground Terminal.

Unpacking

At the time of delivery, be sure to inspect the unit packaging for damage before signing for the shipment. If packaging damage is present, request immediate product inspection and file a claim with the carrier.

Note: Packaging damage does not denote that unit damage exists. If concealed damage is found (damage that is not apparent until the item has been unpacked), stop further unpacking and save all packing for carrier’s inspection. Make a written request for inspection to delivering carrier. This must be done within 15 days after delivery. Then file a claim with the carrier. Do not return goods to the manufacturer without written authorization. up

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If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

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• Direct
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• FAX
• Internet Worldwide Web Home Page Tech Support Email Address Certified Service Web Page

Our Sales Support staff can provide information on pricing and give you quotations. We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you. Our products are listed on the Internet and we can be contacted through our Internet home page. Our Service Support staff can supply technical information about proper setup, operation or troubleshooting of your equipment. We can fill your needs for spare or replacement parts or provide you with on-site service. We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself…over the telephone without a service call. When more extensive service is necessary, we will assist you with direct factory trained technicians or a qualified service organization for on-the-spot repair. If your service need is covered by the warranty, we will arrange for the unit to be repaired at our expense and to your satisfaction. Regardless of your needs, our professional telephone technicians are available to assist you Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by telephone or fax. If you wish to write, our mailing address is:

• Thermo Fisher Scientific (Asheville) LLC 401 Millcreek Road, Box 649 Marietta, OH 45750
• International customers, please contact your local Thermo Scientific distributor.

Quick Start-Up

Locate the storage container in a well ventilated area of the laboratory, with adequate work space available for loading and unloading specimens. Allow for adequate lid opening clearance.

• Warning Nitrogen is a potential asphyxiant and can cause rapid suffocation without warning. Store and use in area with adequate ventilation. DO NOT vent the container in confined spaces. DO NOT enter confined spaces where gas may be present unless area has been well ventilated. Oxygen monitors are recommended. Refer to “Handling Liquid Nitrogen” in the appendix at the end of this manual. up
• Warning During operation, maintain a distance of at least 3 feet from Bypass Vent in Figure 1-1 to prevent harmful LN2 exposure. up
• Warning Ensure there are no physical objects that can contact the open lid and cause an accidental closure. If the unit is not placed against a solid wall, verify a physical barrier is installed at the base to prevent unit movement while working in it. up

Connect Liquid Nitrogen Transfer Hose

The container should be located near the liquid nitrogen supply, allowing enough space for nitrogen source tank replacement. Arrangements should be made to collect the condensate, which will form on the transfer hose. A four foot nitrogen transfer hose with a 1/2″ flare fittings is supplied with Model 7400/7401. A six foot hose is standard with Models 7402/7403, 7404/7405 and 7406/7407. The use of a transfer hose longer than six feet may degrade system performance.

Caution The flair connection on the ends of the transfer hose does not require any sealant. Pipe dope or sealing tape may cause contamination of the liquid fill solenoid, or leaks at the hose connections. up
The storage system requires a user-supplied low pressure-regulated (22 PSIG) liquid nitrogen supply. Anything higher than 22 PSIG will degrade the performance of the CryoPlus storage container. Connect the transfer hose packed with the CryoPlus unit between the low pressure, liquid outlet of the liquid nitrogen supply tank (22 PSIG) and the storage container. After the transfer hose has been connected, open the supply tank valve and check the connections for leaks.

Attach Power Cord

1. Loosen screw located on the power cord retainer. Spread the retainer.
2. Insert the power cord into the power outlet module. Tighten screw on the power cord retainer.
3. Fit the power cord/outlet module assembly into the connection on the unit. Tighten the module screws to secure the cord to the unit.

Connect Lid Strap (Model 7406/7407)

Included with each unit is a lid strap for the user’s convenience.

1. Remove the protective white nylon screws from the areas indicated. Discard these screws.
2. Install the strap as shown below, using the screws included with the strap.

Optional Manual Lid Lock

A manual lid lock is available for each unit to secure the lid while it is open:

1. With the lid open, insert the quick release pin through the hole in the left side Lid Stop Bracket.
2. This lid lock will provide a manual restraint to compliment the lid pneumatic spring(s) installed on the unit.
3. Before closing the lid, remove the quick release pin. It is secured to the Lid Stop Bracket via the lanyard attached to the bracket.

Connect to Electrical Supply

With the power switch turned OFF, connect the unit to a grounded electrical outlet. See the data plate on the back of the unit, or the electrical schematics included in this manual, for voltage and full load amps. The power switch on the back of the unit is the mains disconnect and is also a reset- type circuit breaker. If an overload condition occurs, the built-in circuit breaker will trip and the power switch will turn off. Turning the power switch on resets the circuit breaker. If the circuit breaker trips again within a short time period, the unit should be checked by a qualified electrician.

Warning Use only a grounded electrical receptacle. Failure to ground the unit can result in serious injury. up

Install Optional Platform Riser

Depending on the inventory control system chosen, install the platform riser (if applicable) as shown in Figure 1-4.

Note In the liquid phase, the standard platform remains in the bottom of the tank. up

Install Temperature Sleeve

A Temperature Sleeve is designed to assist the temperature gradient within the unit so that in a normal vapor phase setting of 3-5 inches of liquid, the air temperature encompassed within the sleeve remains below -130°C. The sleeve is standard on all CryoPlus units and is installed when shipped from the factory. When properly installed, the ends of the sleeve are aligned with the temperature probe at the rear of the tank, and the square holes in the bottom of the sleeve are aligned with the fill and pressure ports.

Caution It is imperative that the positioning of the sleeve not block either the fill or liquid level tube orifices located at the bottom rear of the tank. Should blockage occur, it will cause filling and liquid level indicator problems. up

Fill Unit

When shipped from the factory, the liquid nitrogen level settings of all CryoPlus units are set at Vapor Phase settings of five inches high limit, three inches low limit (factory defaults). It is not recommended that these settings be changed until the unit has been filled for the first time and allowed to stabilize.

Caution The lid must remain open throughout the initial filling of the storage container. up

Fill Unit (cont.)

When electric power and LN2 have been connected, open the lid and turn on the power switch to begin filling the unit. Because the unit must go from ambient room temperature to -196°C, considerable boiling of the liquid nitrogen takes place, turning into super-cooled nitrogen gas which flows over the side of the open chamber. As this occurs, frost becomes visible around the top of the unit. This is normal during the initial fill with the lid open and disappears once the unit has stabilized.
As the unit fills, the bar graph on the front of the cabinet monitors the progress by displaying the liquid level (green lights), the high and low limit set points (flashing orange). Refer to Section 2. The storage container fills until the liquid nitrogen reaches the high level set point and the 5-inch flashing orange LED changes to flashing red. The LN2 storage container is now in full automatic operation.
After the initial fill is complete, close the lid and allow the unit to stabilize for a minimum of 8 hours, before changing the high or low level setpoints or adding inventory.

• Caution Some popping or cracking noises may be heard after the unit is initially filled and the lid is opened and closed the first few times. This is normal and quickly disappears. up
• Note If the lid is opened frequently, condensation can occur on the vent port, causing icing of the port. See the maintenance section for defrost information. up

Remote Alarm Connector
The CryoPlus control system provides remote alarm contacts, wired to an RJ-11 connector on the back of the cabinet. Figure 1-5 identifies the pin designations.

RS-232 Interface Connector
The CryoPlus storage system is equipped with an RS-232 Serial Communication Interface for the remote transmission of data. An RJ-11 telephone-style connector is located on the rear of the cabinet. Figure 1-5 identifies the pin designations. The RS-232 provides information to a serial printer or terminal via the following protocol:

• 9600 Baud
• 1 Stop Bit
• No Parity
• 8 Data Bits

Note It is the User’s Responsibility to take reasonable measures to protect the computing devices against viruses and malware infection. up

The user ensures that only users with a valid User ID and password can gain access to the Personal computer. Procedures should be developed and maintained to ensure that users do not share their unique user ID and or password.

Installation Verification

The following procedures test key elements of the CryoPlus Freezer system and verify the unit’s installation. These tests can be performed at the operator’s discretion. If any of these tests fail, contact the Technical Service Department or your local Thermo sales representative.

LN2 Supply
The source tank should indicate that it is full and the pressure to the Cryo unit regulated at 22 PSIG. Check all connections.

Temperature Sleeve
When installed, the ends of the sleeve must align with the temperature probe at the back of the tank and the pressure and fill ports are visible through the square holes at the bottom of the sleeve.

Power Switch
Turn the unit on with the power switch located on the back of the unit. Under normal conditions, all LEDs on the control panel and bar graph, except for the alarm LEDs illuminate for approximately 2-3 seconds. The power switch is the main disconnect for the system.

Keypad
Press each button on the control panel, listening for a “beep” response.

Control Panel Key Switch
Turn the key switch to the Programming Access position (.). Control panel temperature display indicates alarm setpoint temperature. Turn the key to the Lock position and the display shows the actual chamber temperature.

Installation Verification (cont.)

LED Test
Turn the unit off. Turn the key switch to the Programming Access position. Turn the unit on while pressing and holding the Low Level button for four seconds. Press Manual Fill to begin the test. When all LEDs have cycled, turn the unit off to reset the system.

Remote Alarm Contacts
With the contacts wired to a remote alarm, turn the unit on, wait a few seconds, then turn the unit off. The alarm should activate immediately.

Manual Fill
Turn the key switch to the Programming Access position. Press the Manual Fill button. The fill indicator will light and the unit will begin filling the chamber. If the LN2 level is more than one inch below the high-level setpoint, the liquid nitrogen will continue to flow after Manual Fill is released until the high level setpoint is reached. To stop filling before high level, turn the unit off, then on. If LN2 is already in the tank and the level is less than one inch below the high-level setpoint, filling will stop when Manual Fill is released.

Programming Access Test
With the unit turned on, turn the key to the Programming Access posi-tion. The control panel display shows the high-temperature alarm set-point. Press High Level, then the up or down arrow. The setpoint moves accordingly. Press Low Level, then the up or down arrow. The setpoint moves accordingly. Return the key to Lock. The control panel display shows thermocouple temperature and bar graph shows new high and low level setpoints.

Note The unit returns to factory default settings unless setpoints were saved in above tests by pressing Enter. up

Dip Level Test
This test compares the liquid level shown on the bar graph with the actual level in the tank, using the ruler supplied with the unit. With the tank filled and stable, lower the ruler along the edge of the tank until it is at the bottom. (Take subsequent measurements at this same location.) When the LN2 stops boiling, pull out the ruler. The actual liquid level will be approximately one inch lower than the frost line on the ruler. Compare this with the level shown on the bar graph. Measuring tolerance for the low scale (2″ to 7″) is ±1/2 inch. Measuring tolerance for the high scale (1″ to 25″) is ±1 inch.

Caution Some shrinkage of the ruler may occur, depending on the level of liquid nitrogen in the tank. up

Operation

All functions of this CryoPlus storage unit are controlled by a programmable microprocessor. Commands to the control system are given using the control panel on the cabinet lid (Figure 2-1). A multi-coloured bar graph on the front of the cabinet shows the status of the system, indicating the liquid level inside the chamber, and the high level (stop fill) and low level (start fill) points. See Figure 2-2.

Warning Do not use the unit as a work surface. The lid should be closed except during loading and unloading. up

Note By viability assessment protocol, the user assesses the sample viability after the samples are thawed and before its end application. up The elements on the left side of the control panel illuminate when fault conditions occur, providing information about the alarm state. Refer to Section 3 of this manual. A three element alarm bar illuminates during an alarm condition to visually alert the operator. The Silence button is used to silence the audible alarm. Refer to the alarm descriptions and corrective actions in Section 3 of this manual.

Other elements of the control panel are:

• A three digit display, showing the thermocouple temperature when the keyswitch is in the Lock position. The thermocouple junction is located 4½ to 5½ inches below the lid on Models 7400 through 7405, and 5½ to 6½ inches below the lid on Models 7406 through 7407. When the key is in the Programming Access (.) position, the display shows the High Temperature Alarm set point.
• Enter button, used to send programming changes to the microprocessor.
• Up and down arrows that change the high and low level settings when making programming changes, are also used to change the high temperature alarm setpoints.
• High Level button, changing the level at which the system stops filling.
• Low Level button, pressed to change the level at which the system starts filling.
• High Temp button, changing the temperature at which the high temperature alarm activates.
• Manual Fill button, pressed to manually fill the tank. The level must be at least 1-1/4 inches below the high level setpoint to start a manual fill.
• Fill Valve Open light, indicating the fill valve is open.
• Key switch, used to allow program changes when the key is in the (.) Programming Access position and to protect the system from tampering or accidental button presses when the key is in the Lock position.

Bar Graph

Located on the front of the CryoPlus cabinet, the tri-colour, 24-light bar graph displays the liquid level inside the chamber, and (start fill) low-level and (stop fill) high-level setpoints (Figure 2-2). A Scale Select button at the base of the bar graph toggles the scale between the high (liquid phase) and low (vapour phase) scales. Refer to Figure 2-2. Numbers in parentheses are the metric equivalents. The left side scale is for settings from 1-inch to 25-inches in 1 inch increments. The right side scale is for settings from 2-inches to 7 inches in 1/4″ increments (vapour scale). The three light bars at the top of the level panel are visible alarm lights that coincide with the alarms indicated on the top control panel on the lid. The three colors of the bar graph (Figure 2-3) are: Orange (steady) indicates the remaining space above the high-level setpoint.

Orange (flashing): Under normal conditions, the flashing upper LED is the high-level (stop fill) setpoint, and the flashing lower LED is the low level (start fill) setpoint. Green indicates the actual liquid Nitrogen level. Red (steady) indicates the amount of space below or above the setpoint from the actual liquid level. Red (flashing) indicates that the liquid level is above or below the level set points. A possible alarm condition is pending.

A single Red (flashing) after a fill operation indicates that the liquid level is at the high-level setpoint. This is not an alarm condition.

Program Controller

Caution The Key Switch must be in the Programming Access (.) position to program the controller or to access any Touch Pad function. The Controller will automatically return to the lock position, even though the key is in the Access (.) Position, if no entry is made on the keypad within four (4) minutes. On the high scale (left side, 2″- 25″), the high and low-level setpoints must be at least 3″ apart. On the low scale (right side, 1.5″-7.25″), the high and low level setpoints must be at least 1.25″ apart. up

Change High Level (Stop Filling) Setpoint

Refer to Figures 2-1 and 2-2.

1. Turn the key switch to the Programming Access position (.) and verify that the desired scale is selected on the Bar Graph.
2. If the yellow Fill Valve Open indicator is lit, the system is in Fill Mode.
3. Press High Level.
4. Press the up arrow to raise the high-level setpoint. A flashing orange LED will begin to move upward. Release the up arrow when it reaches the desired level on the bar graph. If lowering the high level (stop filling) setpoint, press the down arrow. The flashing orange LED will begin to move downward.
5. Release the down arrow when the stop filling (high level) setpoint is reached.
6. Press Enter to store the new setting in the microprocessor memory. Return the key switch to the Lock position.

Change Low Level (Start Filling) Setpoint

Refer to Figures 2-1 and 2-2.

1. Turn the key switch to the Programming Access position (.) and verify that the Bar Graph is set to the desired scale.
2. If the yellow Fill Valve Open indicator is lit, the system is in the fill mode.
3. Press Low Level.
4. Press the up arrow to raise the low level (start filling) setpoint. A flashing orange LED will begin to move upward. Release the up arrow when it reaches the desired level on the bar graph.
5. If lowering the low level (start filling) setpoint, press the Down Arrow. The flashing orange LED will begin to move downward. Release the Down Arrow when the low level (start filling) setpoint is reached.
6. Press Enter to store the new setting in the microprocessor memory. Return the key switch to the Lock position.

Note Depending on the new setpoints, filling will not begin until the chamber liquid level falls below the new low level setpoint. up

Change High Temperature Alarm Setpoint

The High-Temperature Alarm Setpoint is the temperature at which the high- temperature alarm activates.

1. Turn the key switch to the Programming Access position (.). The digital display will indicate the current High-Temperature Alarm set point.
2. Press High Temp. Three decimal points in the digital display will flash on and off.
3. Press the up arrow to raise the temperature alarm setpoint or the down arrow to lower it.
4. The Enter button must be pressed after the desired high-temperature alarm point is displayed.

When programming is complete, turn the key switch to the Lock position [.]. The thermocouple temperature will be displayed once again, and the controller will now operate at the new setpoint.

Set Microprocessor Internal Clock

The “real time” internal clock enables alarms, program changes, and current system status to be printed relative to the actual time and date of occurrence. This information is made available through the RS-232 data port. The factory default setting is Eastern Standard Time (USA). To set the clock, start with the unit turned off.

1. Turn the key switch to Full Access (.). Press the High Level button on the key pad while turning on the power switch located on the back of the unit.

2. Starting from the bottom of the bar graph, the first LED on the bar graph lights and the numeric display on the control panel shows the current time hundredths of seconds.

3. Press the up arrow (to increase) or down arrow (to decrease) the setting.
   Set Clock (continued)

4. Press the Enter key to lock in the value and advance to the next setting. The chart below shows the settings in sequence.

*LED lights . . . . . . . . . . . . . . . . . . .Clock Setting**

• 1 . . . . . . . . . . . . . . . . . . . . .hundredths of seconds
• 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .seconds
• 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .minutes
• 4 . . . . . . . . . . . . . . . . . . . .hours (in military time)
• 5 . . . . . . . . . . . . . . . . . . . . . . . . . .day of the week
• 6 . . . . . . . . . . . . . . . . . . . . . . . . .day of the month
• 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .month
• 8 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .year
  • LED on the Bar Graph, starting at the bottom of the graph and counting upward.

The Optional Control Printer Kit

Note Install the Modular Line Filter included with the kit into the RS-232 interface connector on the rear of the unit. up
The printer (kit P/N 4000565 US/4000665 EU) provides the following information:

Note All functions and error codes, when printed, include the current LN2 level, temperature, time, and date. up

• Power up
• Auto fill cycle
• Manual fill start and scale selection
• Manual fill stop and scale selection
• Changes in program settings
• Cover opened (tank lid opened)
• All error codes

Note Automatic printing every two hours is the factory default. This feature can be programmed to occur from once every hour to once every 24 hours. Contact the Technical Services Department. up

Troubleshooting the Alarms

Refer to the alarm descriptions, probable causes and corrective action information at the end of this section. Use the above system illustration for reference.

• Warning Potential electrical hazards exist in this equipment. Only qualified persons should perform the instructions and procedures described in this section. up
• Warning Ultra-low temperatures are associated with this equipment. Instructions in this section should only be carried out when using special handing equipment or when wearing special, protective clothing. up

In addition to the protection provided by the power switch/circuit breaker, two North American UL and/or CSA, 125 mA, 250 VAC Slo-Blow fuses (P/N 230173) are located on the microprocessor circuit board. Refer to Figure 3-2. To access these fuses, remove the cabinet back panel.

• Warning This service should only be performed by qualified personnel. up
• Caution Make sure Temperature Probe connector & Bypass probe connecttor are mated to the correct connector on PCBA board as shown in Fig. 3-2. Identify the Bypass probe connector which has red colour label saying “LN2 Bypass”. Failing the above condition, LN2 will start spilling out from Bypass valve. up

Access the control panel circuit board (below) by lifting off the plastic frame surrounding the front of the panel and removing the Phillips screws.

Access the bar graph circuit board (right) by lifting off the plastic frame surrounding the front of the panel and removing the Phillips screws.

DIP Switch Settings

No By-pass| With By-pass
1. Up| 1. Down
2. Down| 2. Up
3. Up| 3. Up

The audible alarm can only be silenced with the key switch in the Programming Access position. Return the key switch to the Lock position after silence. Alarms do not ring back except where noted.

The Alarm can only be silenced with the key switch in the Programming Access position. Return the key switch to the Lock position after silence. Alarms do not ring back except where noted.

Maintenance

Valve maintenance is described below.

Warning These procedures must be performed by qualified service technicians. up

Operation
Normally closed: The valve is closed when the solenoid is de-energized, the valve is open when the solenoid is energized.

Positioning / Mounting
The valve is designed to perform properly when mounted in any position. However, for optimum life and performance, the solenoid should be mounted upright to reduce the possibility of foreign matter accumulating in the core tube area.

Maintenance

Warning Turn OFF the electrical power supply and de-pressurize the valve before making repairs. It is not necessary to remove the valve from the pipeline for repairs. s

Cleaning
Periodic cleaning of all solenoid valves(s) is desirable. The time between cleanings will vary, depending on media and service conditions. Generally, if the voltage to the coil is correct, sluggish valve operation, excessive noise, or leakage will indicate that cleaning is required.

Valve Disassembly and Reassembly
De-pressurize valve and turn OFF the electrical power supply. Proceed in the following manner:

1. Remove the retaining clip and slide the entire solenoid enclosure from the solenoid base sub-assembly.
   Caution When the metal retaining clip disengages, it springs upward. s

2. Unscrew the solenoid base sub-assembly and remove the body gasket, core assembly with rider ring and core spring attached.

3. Clean and assemble in reverse order of disassembly, paying careful attention to the exploded view provided (Figure 4-1) for identification and placement of parts.

Note Use thread sealant, not Teflon tape. Tape could get into valve. up

1. After maintenance, operate the valve a few times to ensure proper opening and closing.

General Cleaning

Protective clothing and gloves should be worn whenever cleaning the inside of this unit. Follow established laboratory procedures. Allow the interior of the unit to warm to ambient and use an appropriate germicide.

Freezing chamber
The interior of the CryoPlus unit is made of high quality stainless steel and should not be cleaned with any cleanser containing chlorine.

Exterior cabinet
Wash the outside of the cabinet with a mild detergent.

Defrosting the Storage Tank
To defrost the storage tank, remove the product and LN2 from the tank. Allow the tank to warm at room temperature.

Caution Do not attempt to speed up LN2 evaporation, or frost removal, by blowing direct heat. Avoid using a heater or blowing compressed air into the storage tank. up

Defrosting the Vent Port
To defrost the vent port, open the lid and allow to warm at room temperature. See Caution above. The opening of the storage container can be loosely covered while the vent port thaws. After the port has thawed, remove all moisture from the vent port area before returning the tank to service.

Gas Springs
The gas springs should be checked periodically, and ideally every six months. The opening force, as measured from the front lip from a closed position, should be below 100 N (22.5 lbf) maximum. If the force is above this value, the gas springs should be replaced. If a force measurement is not possible, the gas springs should be replaced every two years.

Specifications

Series| CryoPlus 1| CryoPlus 2| CryoPlus 3| CryoPlus 4
---|---|---|---|---
LN2 capacity (liters)| 90| 200| 340| 552
Static evaporation rate (liters/day)*| 3| 5| 8| 10
Static Holding Time (days)| 30| 40| 42.5| 55
Exterior Height| 41.0″ (104.1 cm)| 41.0″ (104.1 cm)| 41.0″ (104.1 cm)| 47.0″ (119.4 cm)*
Exterior W x D| 21.5″W x 26.0″ F-B| 28.5″W x 34.0″ F-B| 34.5″W x 41.5″ F-B| 43.5″W x 50.0″ F-B**
 | (54.6 cm x 66.0 cm)| (72.4 cm x 86.4 cm)| (87.6 cm x 105.4 cm)| (110.5 cm x 127.0 cm)
Usable Interior Height| 27.5″ (69.9 cm)| 27.5″ (69.9 cm)| 27.5″ (69.9 cm)| 29.5″ (74.9 cm)
Usable Interior Diameter| 16.0″ (40.6 cm)| 24.0″ (61.0 cm)| 31.0″ (78.7 cm)| 39.5″ (100.3 cm)
Electrical| 100 – 120 VAC, 1 PH, 50/60 Hz, 0.8 FLA (Operating Range 90-132 V)|
200 – 230 VAC, 1 PH, 50/60 Hz, 0.5 FLA (Operating Range 180-253 V)
Utility Connections| 1/2″ 45° Flare| 1/2″ 45° Flare| 1/2″ 45° Flare| 1/2″ 45° Flare
 | 4-foot hose| 6-foot hose| 6-foot hose| 6-foot hose
Weight Empty| 194.0 lbs. (88.0 kg)| 325.0 lbs. (147.4 kg)| 416.0 lbs. (188.7 kg)| 680.0 lbs. (308.4 kg)
Weight Full| 354.0 lbs.(160.6 kg)| 680.0 lbs. (308.4 kg)| 1021.0 lbs.(463.1 kg)| 1620.0 lbs. (734.8 kg)
Shipping Weight| 260.0 lbs. (117.9 kg)| 400.0 lbs. (181.4 kg)| 540.0 lbs. (244.9 kg)| 892.0 lbs. (404.6 kg)
Continuing research and improvements may result in specification changes at any time.

• Static evaporation rates are based on new container performance, no product load, styrofoam plug/lid, and no lid openings. Actual working performance may vary with individual applications, ambient conditions, and/or scale accuracy; excludes supply tank evaporation.

** Add 5.0″ (12.7 cm) for utilities and lid opening
*** 93.5″ (237.5 cm) total unit height of CryoPlus 4 with lid open

Environmental Conditions

The Liquid Nitrogen Storage System is designed to be electrically safe in the following environmental conditions:

• Indoors
• Altitude: Up to 2,000 meters
• Temperature: 5°C to 40°C
• Humidity: 80% RH at or below 31°C, decreasing linearly to 50% RH at 40°C
• Mains Supply Fluctuations: ±10% of nominal.
• Installation Category II 1
• Pollution Degree 2 2
• Class of Equipment I

1. Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply.
2. Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.

Parts List

Models

Stock No.| Description| 7400/7401| 7402/7403| 7404/7405| 7406/7407
 | Tank trim gasket| 4000715| 4000716| 4000717| 4000718
107406| Lid seal gasket| 5-foot| 7-foot| 8.5-foot| 11-foot
120054| Dual wheel caster| 4| 4| 4|
120059| Caster|  |  |  | 4
1950940| Lid tumbler lock| 1| 1| 1| 1
129063| Lid pneumatic spring| 1|  |  |
129064| Lid pneumatic spring|  | 2|  |
129065| Lid pneumatic spring|  |  | 2|
129066| Lid pneumatic spring (210 lbs.)|  |  |  | 2
190526| CryoPlus microprocessor board| 1| 1| 1| 1
195000| Ribbon cable – level to main| 1| 1| 1| 1
195721| Ribbon cable – control board to main| 1| 1| 1| 1
195002| CryoPlus main wiring harness| 1| 1| 1| 1
195007| CryoPlus temperature probe| 1| 1| 1|
195214| CryoPlus temperature probe|  |  |  | 1
195024| 120V valve assy. 7400,7402, 7404, 7406| 1| 1| 1| 1
195025| 220V valve assy. 7401,7403, 7405, 7407| 1| 1| 1| 1
400142| RJ-11 output cable| 1| 1| 1| 1
195027| CryoPlus control panel assembly| 1| 1| 1| 1
195028| CryoPlus level panel assembly| 1| 1| 1| 1
Models

Stock No.| Description| 7400/7401| 7402/7403| 7404/7405| 7406/7407
251008| Pressure relief valve 1/4″ MPT| 1| 1| 1| 1
990027| Magnetic lid gasket| 1|  |  |
990028| Magnetic lid gasket|  | 1|  |
990029| Magnetic lid gasket|  |  | 1|
990031| Magnetic lid gasket|  |  |  | 1
180143| LN2 dip measuring ruler| 1| 1| 1| 1
4000400| 4 foot transfer hose| 1|  |  |
4000401| 6 foot transfer hose|  | 1| 1| 1
195884| Lid closing strap| 1| 1| 1| 1
123037| Lid magnet| 1| 1| 1| 1
4000412| T/C Probe Tubing Replacement Kit (12”)| 1| 1| 1|
4000413| T/C Probe Tubing Replacement Kit (15”)|  |  |  | 1
400142| RJ-11 Cable|  |  |  | 1

THERMO FISHER SCIENTIFIC STANDARD PRODUCT WARRANTY (LN2 Vacuum)
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first year warranty period. During the first year, parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo’s expense, labour included. LN2 Vacuum Integrity is covered for two years. Installation and calibration are not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to the performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty. Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the part beyond the original warranty period. The Technical Services Department must give prior approval for the return of any components or equipment. At Thermo’s option, all non-conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN PLACE OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products. Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance. If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or 1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service and special applications. Outside the USA, contact your local distributor for warranty information.

THERMO FISHER SCIENTIFIC INTERNATIONAL DEALER WARRANTY
The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first-year warranty period. Dealers who stock our equipment are allowed an additional six months for delivery and installation, provided the warranty card is completed and returned to the Technical Services Department. During the first year, parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo’s expense, labour excluded. Installation and calibration are not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction before the performance of any repairs. Expendable items, glass, filters, reagents, tubing, and gaskets are excluded from this warranty. Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the part beyond the original warranty period. The Technical Services Department must give prior approval for the return of any components or equipment. At Thermo’s option, all non-conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination.

THIS WARRANTY IS EXCLUSIVE AND IN PLACE OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products. Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance. Contact your local distributor for warranty information. We’re ready to answer your questions on equipment warranty, oper- ation, maintenance, service and special application.

Regulatory Compliance

Product Safety
This product family has been tested to applicable product standards by UL and TUV SUD, Nationally Recognized Test Laboratories (NRTL).

Electromagnetic Compatibility

Any changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Note This equipment has been tested and found to comply with the limits for a Class A digital device, under part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.

Canadian ISED IC Notice
This class B digital apparatus complies with Canadian ICES-001. Cet appareil numérique de la classe B est conforme á la norme NMB-001 du Canada.

Evaluation of Chemicals – Regulations and Directives

REACH – Europe
We are committed to meeting all compliance obligations to evaluate, communicate, and register any Substances of Very High Concern (SVHC), and finding alternates where appropriate.

RoHS – Europe
We are determined to reduce the impact we have on the environment, and so can declare that this product fully complies with the European Parliament’s RoHS2 (Restriction of Hazardous Substances) Directive 2011/65/EU, with respect to all the following substances:

• Lead (0,1 %)
• Mercury (0,1 %)
• Cadmium (0,01 %)
• Hexavalent chromium (0,1 %)
• Polybrominated biphenyls (PBB) (0,1 %)
• Polybrominated diphenyl ethers (PBDE) (0,1 %)

Evaluation of Chemicals – Regulations and Directives (cont.)

Our compliance is witnessed by written declaration from our suppliers and/or component testing. This confirms that any potential trace contamination levels of the substances listed above are below the maximum level set by the latest regulations or are exempt due to their application.

RoHS – China
This product complies with the requirements of the legislative act Administration on the Control of Pollution Caused by Electronic Information Products (ACPEIP). A label of conformance, such as one of the following, may be found on the product:

Additional Regulations & Markings

European Union
The European voltages of this product meets all the applicable requirements of the European Directives, and display the CE Marking. An EC declaration of conformity may be obtained from the manufacturer.

End-of-life Care
Be sure to follow local regulations when disposing of an old unit. Some suggestions are listed below:

• Remove items and defrost unit. Be sure to clean up any biological safety hazards.
• Remove the cabinet door to help prevent entrapment inside of a unit.

WEEE Compliance

WEEE Compliance. This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the following symbol. Thermo Fisher Scientific has contracted with one or more recycling/disposal companies in each EU Member State, and this product should be disposed of or recycled through them. Further information on our compliance with these Directives, the recyclers in your country, and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www.thermofisher.com/

Handling Liquid Nitrogen

Warning Contact of liquid nitrogen or cold gas with the skin or eyes may cause serious freezing (frostbite) injury. up

Handle liquid nitrogen carefully.
The extremely low temperature can freeze human flesh very rapidly. When spilled on a surface, the liquid tends to cover it completely and intimately, cooling a large area. The gas issuing from the liquid is also extremely cold. Delicate tissue, such as that of the eyes, can be damaged by exposure to the cold gas which would be too brief to affect the skin of the hands or face. Never allow any unprotected part of your body to touch objects cooled by liquid nitrogen. Such objects may stick fast to the skin and tear the flesh when you attempt to free yourself. Use tongs to withdraw objects immersed in the liquid, and handle the object carefully.

Wear protective clothing
Protect your eyes with a face shield or safety goggles (safety glasses without side shields do not give adequate protection). Always wear gloves when handling anything that is, or may have been, in immediate contact with liquid nitrogen. Insulated gloves are recommended, but heavy leather gloves may also be used. The gloves should fit loosely so that they can be thrown off quickly if liquid should splash into them. When handling liquid in open containers, it is advisable to wear high-top shoes. Trousers (which should be cuffless if possible) should be worn outside the shoes.

Introduction
The safe handling and use of liquid nitrogen in cryogenic refrigerators and dewar flasks is largely a matter of knowing the potential hazards and using common-sense procedures based on that knowledge. Two important properties of liquid nitrogen present potential hazards:

1. It is extremely cold. At atmospheric pressure, liquid nitrogen boils at -320° F (-196° C).
2. Very small amounts of liquid vaporize into large amounts of gas. One liter of liquid nitrogen becomes 24.6 cu. ft. (0.7 m3) of gas. The safety precautions in this manual must be followed to avoid potential injury or damage that could result from these two characteristics. Do not attempt to handle liquid nitrogen until you read and fully understand the potential hazards, their consequences, and the related safety precautions. Keep this booklet handy for ready reference and review.

Note Because argon is an inert gas whose physical properties are very similar to those of nitrogen, the precautions and safe practices for the handling and use of liquid argon are the same as those for liquid nitrogen. up

Use containers designed for low-temperature liquids.
Cryogenic containers are specifically designed and made of materials that can withstand the rapid changes and extreme temperature differences encountered in working with liquid nitrogen. Even these special containers should be filled SLOWLY to minimize the internal stresses that occur when any material is cooled. Excessive internal stresses can damage the container. Do not cover or plug the entrance opening of any liquid nitrogen refrigerator or dewar. Do not use any stopper or other device that would interfere with venting of gas.
These cryogenic liquid containers are generally designed to operate with little or no internal pressure. Inadequate venting can result in excessive gas pressure which could damage or burst the container. Use only the loose-fitting necktube core supplied or one of the approved accessories for closing the necktube. Check the unit periodically to be sure that venting is not restricted by accumulated ice or frost.

Use proper transfer equipment.
Use a phase separator or special filling funnel to prevent splashing and spilling when transferring liquid nitrogen into or from a dewar or refrigerator. The top of the funnel should be partly covered to reduce splashing. Use only small, easily handled dewars for pouring liquid. For the larger, heavier containers, use a cryogenic liquid withdrawal device to transfer liquid from one container to another. Be sure to follow the instructions supplied with the withdrawal device. When liquid cylinders or other large storage containers are used for filling, follow the instructions supplied with those units and their accessories.

Do not overfill containers.
Filling above the bottom of the neck tube (or specified maximum level) can result in overflow and spillage of liquid when the neck tube core or cover is placed in the opening.

Never use hollow rods or tubes as dipsticks.
When a warm tube is inserted into liquid nitrogen, liquid will spout from the top of the tube due to gasification and rapid expansion of liquid inside the tube.

Warning Nitrogen gas can cause suffocation without warning! up

Store and use liquid nitrogen only in a well-ventilated place.
As the liquid evaporates, the resulting gas tends to displace the normal air from the area. In closed areas, excessive amounts of nitrogen gas reduce the concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colourless, odorless and tasteless, it cannot be detected by the human senses and will be breathed as if it were air. Breathing an atmosphere that contains less than 18% oxygen can cause dizziness and quickly result in unconsciousness and death.

Note The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture; not the gas itself. The issuing gas is invisible. up

Never dispose of liquid nitrogen in confined areas or places where others may enter. Disposal of liquid nitrogen should be done outdoors in a safe place. Pour the liquid slowly on gravel or bare earth where it can evaporate without causing damage. Do not pour the liquid on pavement.

First Aid
If a person seems to become dizzy or loses consciousness while working with liquid nitrogen, move to a well-ventilated area immediately. If breathing has stopped, apply artificial respiration. If breathing is difficult, give oxygen. Call a physician. Keep warm and at rest.
If exposed to liquid or cold gas, restore tissue to normal body temperature (98.6°F) as rapidly as possible, followed by protection of the injured tissue from further damage and infection. Remove or loosen clothing that may constrict blood circulation to the frozen area. Call a physician. Rapid warming of the affected part is best achieved by using water at 108°F. Under no circumstance should the water be over 112°F, nor should the frozen part be rubbed either before or after rewarming. The patient should neither smoke nor drink alcohol.

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• 401 Millcreek Road
• Marietta, Ohio 45750
• United States

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