Vabiooth VB-M004 Professional Microscope Instruction Manual
- June 14, 2024
- Vabiooth
Table of Contents
Vabiooth VB-M004 Professional Microscope
- This operation manual is for the Model VB-M004 VB-M005 microscope. We recommend that you carefully review this manual prior to operating this device in order to optimize safety and performance and to become fully familiar with its use. Keep this operation manual in an easily accessible place for future reference.
- WARNING: To prevent fire and electrical shock, do not expose the unit to rain or moisture.
- CAUTION: No user-serviceable parts inside. Refer servicing to qualified service personnel.
Before use
Operation
- As the microscope is a precision instrument, always use it with care. Avoid dropping or shaking during the operation. Make sure the microscope is placed on a flat surface.
- Do not put the microscope in direct contact with the sun. Do not expose the microscope to high temperature, damp, or dusty environments.
- When moving the microscope, you should use both hands, typically one hand under the base and one hand on the arm (stand) of the microscope, and set it down carefully.
- CAUTION: It will damage the microscope if you hold the microscope either by the stage, focusing knob or head.
- Be sure the microscope is unplugged before replacing the bulb or fuse and wait until the bulb has cooled down.
- This microscope has a built-in voltage transformer and can be used with a wide voltage range from 110 to 240V.
- Only use the power cord supplied with the microscope.
Maintenance
- Wipe the lens gently with a soft lens tissue. After every use with oil or fingerprints clean the lens surfaces with lens paper or lens paper moistened with a little rubbing alcohol. If you do not have lens paper make sure to use a lint-free towel or cloth.
- CAUTION: Alcohol is flammable. Do not place this chemical near fire or fire-causing source. You should use this chemical in a ventilated place.
- Don’t use an organic solution to wipe the surfaces of the other components.
- If the microscope comes in contact with a liquid, then you should turn off the power immediately and wipe it dry.
- Never disassemble or service the microscope yourself.
- After using, cover the microscope with a dust cover.
Diagram
Microscope Diagram
Assembly
Install the Objectives
- Install the objective into the microscope nosepiece from the lowest magnification to the highest, in a clockwise direction.
Insert the Eyepieces
- Remove the eyepiece tube covers.
- Insert the eyepiece into the tube completely.
Install plug
Insert one side of the plug into the back pocket of the microscope.
Operation
Turn on
- Insert the plug into an outlet and turn it on.
- Adjust the light adjustment knob until you get the suitable brightness. (See fig.1)
Set the specimen slide
- Push the arm of the specimen holder back.
- Insert the slide with the cover glass facing up and allow the arm of the specimen holder forward (See Fig. 2)
- Rotate the X and Y-axis knobs on the mechanical stage to move the specimen to the center (the center of the objective).
Operation
Adjust focus
- Move the objective 4X into the light path.
- Rotate the coarse-focusing knob until the image appears.
- Turn the fine-focusing knob to make the specimen clearer. (See fig.3)
- When using the 4X and 10X objectives, open the aperture diaphragm and field diaphragm to the widest position.
Adjust the tension of the focus
- If the course focus knob is difficult to turn when focusing or the stage drifts downwards by itself, you can solve the problem by adjusting the tension adjustment ring.
- Turn clockwise to tighten the controls or counterclockwise to loosen the controls (See Fig. 4)
Adjust the interpupillary distance
- When observing with both eyes hold the bases of the base of the eyepieces and rotate them around the axis until there is only one field of view. (See Fig. 5).
- On the left and right eyepiece base should point to the same number.
- The number is the interpupillary distance.
Adjust diopters
- Rotate one eyepiece to the “0” position using the white thread. This should be done to your dominant eye, then using that same (one side only) eyepiece focus on a specimen clearly.
- Now observe the other eyepiece and rotate its diopter adjusting the ring until the image is clear in that eyepiece. (See fig.6)
Adjust diaphragm
- The diaphragm can prevent other light from entering and strengthen the image by limiting the beam of light entering the condenser. When the image of the diaphragm is just on the edge of the field of view, you can obtain the clearest image.
Adjust aperture diaphragm ②
- The aperture diaphragm decides the numerical aperture of the illumination. If the N.A. of illum i nation matches with the N.A. of the objective②, you can obtain better resolution and contrast.
- If the contrast is low, you can adjust the N.A. of the condenser to 70%-80% of the N.A. of the objective when using. Adjust the upper diaphragm ring until you see the image above (See Fig. 7).
- Use of a diaphragm and put the N.A. of condenser ② to 80% of the objective. e.g.: objective 40X (N. A. =0.65), put the aperture diaphragm to about 0.65X0.8=0.52. (See fig.8)
Use the oil objective
- Use the objective 40X to focus the specimen.
- Put a drop of oil on the specimen. (See fig.9)
- Rotate the nosepiece to place the 100X objective (oil) into the light path. Then use the fine focusing knob to focus us.
- Make sure there is no air bubble in the oil.
- A. Take out the eyepieces to examine the air bubble. Open the aperture diaphragm and field diaphragm fully and observe the edge of the objective from the tube without the eyepiece.
- B. You can rotate the nosepiece slightly and swing the oil objective sometimes to remove the air bubble.
- After using oil, clean the 100X lens with clean lens paper using rubbing alcohol if the oil does not come off easily.
Specification
Microscope e general specifications
Troubleshooting
| Symptom | Cause | Remedy |
|---|
Optics
(1)The lamp is bright, but the light in the field of view is not bright
enough.| The field diaphragm is not large enough.| Enlarge the field
diaphragm.
The condenser is too low.| Adjust the condenser.
The condenser is not centered.| Center the condenser.
(2)The side of the field of view is dark or not even.| The nosepiece is not in
the right position.| Turn the nosepiece into the right position.
Stain or dust has accumulated on the condenser, objective, eyepieces, and base
lens.| Clean the lens.
Condenser adjustment needed (see step 3-7)| Center the condenser.
(3)Stain or dust is observed in the field of view.| Stains have accumulated on
the specimen.| Clean the specimen.
Stains have accumulated on the lens.| Clean the lens.
(4)Unclear image| No cover glass on the specimen slide.| Add the cover glass.
The cover glass is not standard.| Use a standard cover glass with
thickness 0.17mm.
The cover glass faces down.| Put the cover glass to face up.
The immersion oil has accumulated on the dry objective.| Clean thoroughly.
The immersion oil is not used for oil objective 100XR.| Use immersion oil.
Air bubbles in the immersion.| Get rid of the air bubble.
Use the wrong immersion oil.| Use correct one.
The aperture is not opened correctly.| Adjust the iris diaphragm.
Stain or dust has accumulated on the lens in the inlet of the head.| Clean the
lens.
The condenser is not in the right position.| Adjust the condenser.
(5)One side of the field of view is dark or the image moves while focusing.|
The specimen slide is not fixed.| Fix with clips.
The nosepiece is not in the right position.| Turn the nosepiece into the right
position.
The condenser is centered incorrectly.| Center the condenser.
(6) The eyes feel tired easily. The right field of view doesn’t superpose with
the left.| The interpupillary distance is wrong.| Adjust the interpupillary
distance
The diopter adjustment is wrong.| Adjust the diopter.
The eyepiece on the right is different from the left.| Use the same eyepieces.
Symptom| Cause| Remedy
---|---|---
Mechanics
(1)Can not get the objective focused.| The cover glass faces down.| Put the
cover glass to face up.
The cover glass is not standard.| Use a standard cover glass with a thickness
of 0.17mm.
(2) The objective touches the cover glass while turning
the nosepiece.
| The cover glass faces down.| Put the cover glass to face up.
The cover glass is not standard.| Use a standard cover glass with a thickness
of 0.17mm.
(3)Coarse focusing knob is too tight.| Tension knob is too tight.| Loosen a
little.
(4)Stage declines itself and can’t stay in
the focus plane.
| The tension knob is too loose.| Tighten a little.
(5)Coarse focusing knob can’t rise.| The limit stop knob is locked.| Loosen
the knob.
(6)Coarse focusing
knob can’t decline.
| The base of the condenser is too low.| Raise the base.
__
(7)Can not move the slide smoothly.
| The slide is not fixed correctly.| Adjust it correctly.
The movable specimen holder is not fixed properly.| Adjust it correctly.
(8)The image moves obviously when touching the stage.| __
The stage is fastened incorrectly.
| __
Fasten the stage correctly.
Electrical
__
(1)The bulb does not work.
| No power supply.| Check the connection of the power cable.
The bulb is not inserted correctly.| Insert it correctly.
The bulb burnt out.| Replace it.
(2)The bulb burnt out usually.| Use the wrong bulb.| Replace with the correct
one.
(3)The field of view is not bright enough.| Use the wrong bulb.| Replace with
the correct one.
The use of a light adjustment knob is wrong.| Adjust correctly.
(4)The bulb flickers or the brightness is not stable.| The bulb will burn out
soon.| Replace with a new one.
The wire doesn’t connect all right.| Connect correctly.
- Vabiooth provides an 18-month warranty and lifetime free service.
- If you encounter any issues with the product, Please contact the Vabiooth team to promptly receive troubleshooting tips or a replacement.
Contact us
- [email protected]
- +86 17306342950
- Vabiooth (How-to videos, warranty details)
FAQ
- How to install the microscope?
- Please refer to our user manual. In addition, we have recorded an installation video for each machine. Please search for “Vabiooth” on YouTube to watch.
- What are the options for eyepieces?
- To provide a better customer experience, all eyepieces have been upgraded to WF25X eyepieces, and if you need WFIOX, WFI6X, or 2X Teleconverter, Please contact us.
- How do I prevent dirt and grime from getting into the eyepieces and objectives?
- Remind the user to use the Vabiooth Microscope Cover to keep your microscope covered all the time when not in use. This will help keep the eyepieces and objectives from water and dust. If you need the microscope cover, please contact us.
- Why are your machines divided into so many versions?
- We want to improve the display’s color restoration & clarity ability to show perfect imaging for different applications, our microscopes are mainly divided into blood version, constant temperature version, and general version (Different versions use different chips). The blood version is used to observe blood cells. The constant temperature version is suitable for breeding and farms to artificial insemination (35-36 degrees is the most active temperature for sperm), and the universal version is suitable for experimental research, and veterinarians to observe pond organisms, parasites, and soil.