ZEISS L4.I Microscope Installation Guide
- June 13, 2024
- Zeiss
Table of Contents
L4.I Microscope
Installation Guide
L4.I Microscope
THE CONTAFLEX ON THE MICROSCOPE
The CONTAFLEX on the microscope
Instruction for use holding ring and intermediate ring
Photomicrographs can easily be made with the CONTAFLE X, since the taking lens
is also the finder lens of this single lens reflex camera. This is very
convenient in so far as there is no neces-sity for an additional observing
tube or a focus-ing ocular. Only a holding and an intermediate ring are
necessary and, of course, the bracket used for attaching of the CONTAPLE X
converter lenses.
The holding ring is slipped over the eyepiece tube (see ill.) and clamped
tight with.the milled screw (1). The attachment bracket must first be pushed
from below on to the grooves at both sides of the lens of the CONTAFLEX until
it snaps in audibly, then the intermediate ring is screwed tightly into it.
(To remove the bracket press the chromium-plated locking catches on both
sides). The microscope is focused as usual. Then attach the CONTAFLEX to it
and fasten it by means of the milled screw (2) on the holding ring. 
The camera lens
should be focused to 00 and the diaphragm opened to the full aperture (1:
2.8).
Stopping down tht’ camera lens will not alter the brightness of t,he Hnder
image but may lead to trouble in other respects.
Observing the finder image closely the holding ring is slowly moved a little
higher until the finder image or the circular image area are illuminated as
uniformly as possible. For this purpose the milled screw (1) must be loosened
and tightened once again when focusing is completed.
Fine focusing is then earried out by means of the coarse and fine adjustment
of the micro scope. This can be examined in the viewfinder of the CONTAFLEX .
If the microscopic image is bright, the ground-glars screen ring can beused.
(The split-image rangefinder is unsuitable on account of the small aperture
resulting from the use of the microscope.) If the finder image appears too
dark in the ground-glass screen ring, the correct focusing can be determined
by a comparison with the fine rings of the Fresnel
lens, which are visible in the viewfinder.
Good photomicrographs of suitable contrast are in general possible only when
the illumination is correct eccording to Kohler’s principle. Other wise
disturbing straylight which decreases contrast may occur on the specimen
slide as well as in the microscope tube.
To achieve a good and unifc rm illumination of the finder image, the diaphragm
of the camera lens must be placed on the same spot as is occupied by the pupil
of the human eye when the microscope is Ilsed for visual observation.
With regard to the measurements of the camera lens only oculars are suitable
which have been computed for a relatively great ocular distance.
Furthermore, the ocular of the microscope must yield a plane image in order to
produce a pic ture sharp up to the corners on the film, which lies plane in
the camera. For use with the CONTA FLE X we recommend the Compensation Plan
Ocular KPL 12.5 x lI f Mess!; . Zeiss (finkel, G6ttingen, if it proves
impossible to obtain a uniform illumination and sufficient marginal de
finition with the existing ocular. When the KPL 12.5 x ocular is used · the
upper ring bear ing the inscription must be unscrewed (see ill.).
It is also possible to use photographic oculars with correction adjustment.
They must be ad justed to .
The reproduction size of the fim of the CONTAFLFX is appro½ of that of the
microscope magnification (exactly 1: 5.5 ) If a CONTAFLEX photomicrograph is
enlarged 5.5 x and viewed from a distance of 250mm . the impression will bethe
same as that of visual microscopy.
For further particulars and advice consult YOll photographic dealer or the
department “Wissen schaftliche Contax- amd Contaflex-Photo graphie” of Z
EISS IKON AG , Stuttgart-S, Dorn haldenstrasse 5.
ZEIS S IKON AG. STUTTGART
lenglischl 019 / 1525
Printed in Germony 1 0855
Author : J. Kraatz