ZEISS L4.I Microscope Installation Guide

: June 13, 2024
: Zeiss

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L4.I Microscope
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L4.I Microscope
Installation Guide

L4.I Microscope

THE CONTAFLEX ON THE MICROSCOPE

The CONTAFLEX on the microscope
Instruction for use holding ring and intermediate ring
Photomicrographs can easily be made with the CONTAFLE X, since the taking lens is also the finder lens of this single lens reflex camera. This is very convenient in so far as there is no neces-sity for an additional observing tube or a focus-ing ocular. Only a holding and an intermediate ring are necessary and, of course, the bracket used for attaching of the CONTAPLE X converter lenses.
The holding ring is slipped over the eyepiece tube (see ill.) and clamped tight with.the milled screw (1). The attachment bracket must first be pushed from below on to the grooves at both sides of the lens of the CONTAFLEX until it snaps in audibly, then the intermediate ring is screwed tightly into it. (To remove the bracket press the chromium-plated locking catches on both sides). The microscope is focused as usual. Then attach the CONTAFLEX to it and fasten it by means of the milled screw (2) on the holding ring. ZEISS
L4.I Microscope - overview The camera lens should be focused to 00 and the diaphragm opened to the full aperture (1: 2.8).
Stopping down tht’ camera lens will not alter the brightness of t,he Hnder image but may lead to trouble in other respects.
Observing the finder image closely the holding ring is slowly moved a little higher until the finder image or the circular image area are illuminated as uniformly as possible. For this purpose the milled screw (1) must be loosened and tightened once again when focusing is completed.
Fine focusing is then earried out by means of the coarse and fine adjustment of the micro scope. This can be examined in the viewfinder of the CONTAFLEX . If the microscopic image is bright, the ground-glars screen ring can beused. (The split-image rangefinder is unsuitable on account of the small aperture resulting from the use of the microscope.) If the finder image appears too dark in the ground-glass screen ring, the correct focusing can be determined by a comparison with the fine rings of the Fresnel
lens, which are visible in the viewfinder.
Good photomicrographs of suitable contrast are in general possible only when the illumination is correct eccording to Kohler’s principle. Other wise disturbing straylight which decreases contrast may occur on the specimen slide as well as in the microscope tube.
To achieve a good and unifc rm illumination of the finder image, the diaphragm of the camera lens must be placed on the same spot as is occupied by the pupil of the human eye when the microscope is Ilsed for visual observation.
With regard to the measurements of the camera lens only oculars are suitable which have been computed for a relatively great ocular distance.
Furthermore, the ocular of the microscope must yield a plane image in order to produce a pic ture sharp up to the corners on the film, which lies plane in the camera. For use with the CONTA FLE X we recommend the Compensation Plan Ocular KPL 12.5 x lI f Mess!; . Zeiss (finkel, G6ttingen, if it proves impossible to obtain a uniform illumination and sufficient marginal de finition with the existing ocular. When the KPL 12.5 x ocular is used · the upper ring bear ing the inscription must be unscrewed (see ill.).
It is also possible to use photographic oculars with correction adjustment. They must be ad justed to .
The reproduction size of the fim of the CONTAFLFX is appro½ of that of the microscope magnification (exactly 1: 5.5 ) If a CONTAFLEX photomicrograph is enlarged 5.5 x and viewed from a distance of 250mm . the impression will bethe same as that of visual microscopy.
For further particulars and advice consult YOll photographic dealer or the department “Wissen schaftliche Contax- amd Contaflex-Photo graphie” of Z EISS IKON AG , Stuttgart-S, Dorn haldenstrasse 5.