Attogene AU2030 Cadmium Lateral Flow Kit User Manual

: June 9, 2024
: Attogene

Table of Contents

AU2030 Cadmium Lateral Flow Kit
Product Information
Kit Contents
Product Usage Instructions
- Sample Evaluation
Additional Analysis
Intended Use
Introduction
References
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AU2030 Cadmium Lateral Flow Kit

Product Information

The Cadmium Lateral Flow Kit is a product by Attogene, a biotechnology company based in Austin, Texas. The kit is designed for the screening of Cadmium in water samples at 5 ppb. It is intended for research use only and is not to be used in diagnostic procedures. The kit comes in a format of 10 test cassettes and has a run time of 15-30 minutes.

Kit Contents

Cadmium Cassette – 10 each
Disposable Pipette – 10 each
Sample Dilution Buffer – 5 each
Negative Control – 5 each
Water Sample Bottle – 5 each
Manual – 1 each

Product Usage Instructions

Sample Evaluation

A. Sample:

Using the transfer pipet, remove 150 L of sample from the 125mL water sample bottle.
Transfer 150 L of the sample into one of the sample dilution buffer tubes.
Mix by pipetting up and down 3 times.
Using the transfer pipet, add the diluted sample directly into the sample port of the cassette.

B. Negative Control:

Use the transfer pipet to transfer 150 L of Negative Control into the sample port of the cassette.
Incubate for 15-30 minutes.
Read results.

Additional Analysis

If necessary, positive samples can be confirmed by other conventional methods. A lateral flow reader can also be employed to generate numerical readings from the visual result.

Intended Use

For the screening of Cadmium in water samples at 5 ppb. Samples requiring regulatory action should be confirmed by other conventional methods.

Introduction

Attogene’s Cadmium Lateral Flow Kit can be used to detect Cadmium.
Format: 10 test cassettes, Run Time: 15-30 minutes

Cadmium contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Cadmium accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking cadmium-contaminated food and water include numerous negative health effects. Attogene’s Cadmium Lateral Flow test gives results conforming of 5ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 15-30 minutes.

Kit Contents

Component Name	Volume	Storage
Cadmium Cassette	10 each	RT
Disposable Pipette	10 each	RT
Sample Dilution Buffer	5 each	RT
Negative Control	5 each	RT
Water Sample Bottle	5 each	RT
Manual	1 each	RT

Storage and Stability

The kit should be stored at 2℃ – 30℃ until ready to use.
The test must remain in the sealed pouch until use.

Required Materials Not Supplied

Timer – For timing use

Precautions

The Cadmium Lateral Flow Kit provide only preliminary qualitative test results. Use another, more quantitative, analytical method such as instrumental analysis to obtain a confirmed quantitative analytical result.
Prior to use, ensure that the product has not expired by verifying that the date of use is prior to the expiration date on the label.
The test cassettes are individually packaged in a foil pouch with a desiccant & disposable pipette.
Avoid cross-contamination of samples by using a new bottle and disposable pipette for each sample.
It is good laboratory practice to use positive and negative controls to ensure proper test performance. Samples which do not contain Cadmium (negative controls) as well as samples containing known quantities of Cadmium (positive controls) should be analyzed with each lot of test strips to provide a reference for line intensity to be expected.

Water Collection and Storage

Collect water samples into the Water Sample Bottle included in the kit and store refrigerated for up to 5 days. If samples must be held for greater than 5 days, samples should be stored frozen.
Allow the test cassettes, running buffer, and samples to reach room temperature before testing.
Open two sealed cassettes and remove the 150ul transfer pipets. Perform A and B for each sample evaluation.

A. Sample:

Using the transfer pipet remove 150 μL of sample from the 125mL water sample bottle
Transfer 150 μL of the sample into one of the sample dilution buffer tubes
Mix by pipetting up and down 3 times.
Using the transfer pipet add the diluted sample directly into the sample port of the cassette.

B. Negative Control:

Use the transfer pipet to transfer 150 μL of Negative Control into the sample port of the cassette.
Incubate for 15-30 minutes.
Read results.

Interpretation of Results
For samples prepared as described above, screening concentrations are determined by comparison of the intensity of the test line to the intensity of the control line on parallel test strips. Although control line intensity may vary, a visible control line must be present for results to be considered valid. Test strips with a test line which is darker than or of equal intensity to the test line of the control indicates a result which is below the limit of detection of the test. Test strips with a test line which is lighter than the control strip indicates a result which is ≥ 5 ppb. Test strips with no test line visible (only the control line is visible) indicates a result which is ≥ 50 ppb. Results should be determined within 15-30 minutes after completion of the strip test procedure. Determination made using strips which have dried for more or less than the required time may be inaccurate, as line intensities may vary with drying time.

The appearance of test strips may also be compared to the illustration to determine approximate sample concentration ranges. Please note that the illustration is intended for the demonstration of test line to control line intensity only. Results should not be determined by comparing the intensity of test lines from test strips to the test line intensity of the illustration, as the overall intensity of test strips may vary slightly with different lots of reagents. To obtain semi-quantitative results in the range of 0 – 20 ppb, solutions of known Cadmium concentration (control solutions) must be tested concurrently with samples. Sample test line intensities can then be compared with control solution test line intensities, yielding approximate sample concentrations. Do not use strips run previously to determine semi- quantitative sample concentrations, as test line intensities may vary once strips are completely dry.

Additional Analysis
If necessary, positive samples can be confirmed by other conventional methods. A lateral flow reader may also be employed to generate numerical readings from the visual result.

Who we are:
Attogene is a biotechnology company located in Austin, Texas. Our focus is to enhance health and wellness by offering and developing customer focused Life Science Products domestically and internationally.

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