Attogene B1 Lateral Flow Test Kit Instructions
- June 1, 2024
- Attogene
Table of Contents
Aflatoxin Lateral Flow Kit
Catalog Number: AU2051
For Research Use Only. Not for use in Diagnostic Procedures.
Intended Use
For the screening of Aflatoxin at or above 2 ppb in grain samples.
Introduction
Attogene’ s Aflatoxin B1 Lateral Flow Kit can be used to detect Aflatoxin in
extracted samples.
Format: – Run Time: 15 Minutes
Kit Contents
Component Name | Volume | Storage |
---|---|---|
Aflatoxin B1 Cassette | 10 each | RT |
Sample Extraction/Running Buffer | 50 mL | RT |
Sample Extraction Tube | 10 each | RT |
200ul fixed volume pipette | 10 each | RT |
Storage and Stability
- The kit should be stored at room temperature until ready to use.
- The test must remain in the sealed pouch until use.
Required Materials Not Supplied
- Timer – For timing use
- Marker – for labeling
Extraction
- Collect the sample using the Sample Collection Bottle
- Remove 1gram of ground sample and add into the extraction tube
- Apply 2ml of sample extraction/running buffer.
- Vortex or shake for 3 minutes.
- Let the solution settle for 2 minutes.
- Use 200ul for the assay as described below.
Precautions
- The Aflatoxin B1 Lateral Flow Kit provides preliminary qualitative test results. Using a lateral flow reader can add quantitative determination and numerical readouts. Instrumental analysis such as HPLC can also be used to obtain a confirmed quantitative analytical result.
- Prior to use, ensure that the product has not expired by verifying that the date of use is prior to the expiration date on the label.
- The test cassettes are individually packaged in a foil pouch with a desiccant.
- Avoid cross-contamination of samples by using a new bottle for each sample.
- Use only Aflatoxin Lateral Flow Kit reagents from one kit lot, as they have been adjusted in combination.
- It is good laboratory practice to use positive and negative controls to ensure proper test performance. Samples which do not contain Capsaicin (negative controls) as well as samples containing known quantities of Aflatoxin (positive controls) may be analyzed with each lot of test strips to provide a reference for line intensity to be expected.
Procedure
- Using gloves, remove each lateral flow cassette from the foil pouch. A marker may be used to write on the plastic cassette if desired.
Perform A and B for each sample evaluation starting with the negative control first.
A. Negative Control (perform first):
o Transfer 200ul of the Negative Control (Sample Extraction/Running Buffer) directly into
the sample port of the cassette with the 200ul fixed volume pipette.
o Set a timer for 15 minutes.
B. Sample:
o Transfer 200ul of the extracted sample directly into the sample port of the cassette.
o Set a timer for 15 minutes.
o Read the cassette in a lateral flow reader or by eye
Interpretation of Results
For samples prepared as described above, screening concentrations are
determined by comparison of the intensity of the test line to the intensity of
the control line on parallel test strips. Although control line intensity may
vary, a visible control line must be present for results to be considered
valid. Test strips with a test line which is darker than or of equal intensity
to the test line of the control indicate a result which is below the limit of
detection of the test. Test strips with a test line which is lighter than the
control strip indicates a result which is ≥ 2ppb. Test strips with no test
line visible (only the control line is visible) indicates a result which is ≥
20 ppb. Results should be determined within 15 minutes after completion of the
strip test procedure. Determination made using strips which have dried for
more or less than the required time may be inaccurate, as line intensities may
vary with drying time.
To obtain semi-quantitative results in the range of 0 – 20 ppb, solutions of
known Aflatoxin B1 concentration (control solutions are not supplied with the
kit) may be tested concurrently with samples. Sample test line intensities can
then be compared with control solution test line intensities, yielding
approximate sample concentrations. Do not use strips run previously to
determine semi-quantitative sample concentrations, as test line intensities
may vary once strips are completely dry.
Additional Analysis
If necessary, positive samples can be confirmed by ELISA, HPLC, or other
conventional methods.
A lateral flow reader may also be employed to generate numerical readings from
the visual result.
Contact us if you have any questions.