Attogene AU2029 Mercury Lateral Flow Kit Instruction Manual

: June 5, 2024
: Attogene

Table of Contents

Intended Use
Introduction
Kit Contents
Storage and Stability
Required Materials Not Supplied
Precautions
Water Collection and Storage
Procedure
Interpretation of Results
Additional Analysis
References
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AU2029 Mercury Lateral Flow Kit
Instruction Manual
Mercury Lateral Flow Kit
Catalog Number: AU2029

For Research Use Only. Not for use in Diagnostic Procedures.

Intended Use

For the screening of Mercury in water samples at 10 ppb. Samples requiring regulatory action should be confirmed by other conventional methods.

Introduction

Attorney’s Mercury Lateral Flow Kit can be used to detect Mercury.
Format: 10 test cassettes, Run Time: 5 Minutes
Mercury contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Mercury ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking Mercury-contaminated food and water include brain, heart, kidney, lungs, and immune system problems for adults and physical and mental development delays in infants and children. Attorney’s Mercury Lateral Flow test gives results conforming of 10ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 5 minutes.

Kit Contents

Component Name	Volume	Storage
Mercury Cassette	10 each	RT
Disposable Pipette	10 each	RT
Sample Dilution Buffer	5 each	RT
Negative Control	5 each	RT
Water Sample Bottle	5 each	RT
Manual	1 each	RT

Storage and Stability

The kit should be stored at 2 – 30 until ready to use.
The test must remain in the sealed pouch until use.

Required Materials Not Supplied

Timer – For timing use

Precautions

The Mercury Lateral Flow Kit provides only preliminary qualitative test results. Use another, more quantitative, an analytical method such as instrumental analysis to obtain a confirmed quantitative analytical result.
Prior to use, ensure that the product has not expired by verifying that the date of use is prior to the expiration date on the label.
The test cassettes are individually packaged in a foil pouch with a desiccant & disposable pipette.
Avoid cross-contamination of samples by using a new bottle and disposable pipette for each sample.
It is good laboratory practice to use positive and negative controls to ensure proper test performance. Samples that do not contain Mercury (negative controls), as well as samples containing known quantities of Mercury (positive controls), should be analyzed with each lot of test strips to provide a reference for line intensity to be expected.

Water Collection and Storage

Collect water samples into the Water Sample Bottle included in the kit and store refrigerated for up to 5 days. If samples must be held for greater than 5 days, samples should be stored frozen.
Allow the test cassettes, running buffer, and samples to reach room temperature before testing.

Procedure

Open two sealed cassettes and remove the 200ul transfer pipets. Perform A and B for each sample evaluation.

A. Sample:
o Using the transfer pipet remove 200 L of sample from the 125mL water sample bottle o Transfer 200 L of the sample into one of the sample dilution buffer tubes o Mix by pipetting up and down 3 times.
o Using the transfer pipet add the diluted sample directly into the sample port of the cassette.
B. Negative Control:
o, Use the transfer pipet to transfer 200 L of Negative Control into the sample port of the cassette.
o, Set a timer for 5 minutes.
o, Read results.

Interpretation of Results

For samples prepared as described above, screening concentrations are determined by comparison of the intensity of the test line to the intensity of the control line on parallel test strips. Although control line intensity may vary, a visible control line must be present for results to be considered valid. Test strips with a test line that is darker than or of equal intensity to the test line of the control indicate a result that is below the limit of detection of the test. Test strips with a test line that is lighter than the control strip indicate a result that is 10 ppb. Test strips with no test line visible (only the control line is visible) indicate a result that is 50 ppb. Results should be determined within 5 minutes after completion of the strip test procedure. A determination made using strips that have dried for more or less than the required time may be inaccurate, as line intensities may vary with drying time.

The appearance of test strips may also be compared to the illustration to determine approximate sample concentration ranges. Please note that the illustration is intended for the demonstration of the test line to control line intensity only. Results should not be determined by comparing the intensity of test lines from test strips to the test line intensity of the illustration, as the overall intensity of test strips may vary slightly with different lots of reagents. To obtain semi-quantitative results in the range of 0 20 ppb, solutions of known Mercury concentration (control solutions) must be tested concurrently with samples. Sample test line intensities can then be compared with control solution test line intensities, yielding approximate sample concentrations. Do not use strips run previously to determine semi-quantitative sample concentrations, as test line intensities may vary once strips are completely dry.

Additional Analysis

If necessary, positive samples can be confirmed by other conventional methods. A lateral flow reader may also be employed to generate numerical readings from the visual result.
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