NANOPORE PromethION A100 User Guide

NANOPORE PromethION A100 User Guide

This Quick start guide contains everything you need to set up your Promotion™ 24/48 (A100) and to check the device is
ready for use.

For detailed information and troubleshooting, view the User Manual.

Pre-installation
Before using the device, familiarise yourself with the following

Promotion 24/48 (A100) User Manual

community.nanoporetech.com/to/promethioncommunity.nanoporetech.com/to/safety

Safety and regulatory information

**What’s in the box:***

• Peripherals not supplied: monitor (Display port or VGA compatible), USB keyboard, USB mouse, Ethernet cable.

1 Set up and power on your device

Data Acquisition Unit A100
Device connections

1. 10G Ethernet ports
2. PCIe cable ports
3. USB-B port
4. USB-Mini ports
5. USB-A ports
6. USB-C port
7. Power sockets
8. Power on/off- Sequencing Unit
9. Power on/off- Data Acquisition Unit
10.  Fibre module cages
11. Mini display ports
12. VGA port

Sequencing Unit

1. Unbox your Prom ethion
   Place the Sequencing Unit and Data Acquisition Unit on a bench less than 2 m from each other. Ensure 30 cm clearance around each unit.

2. Attach network cables
   Connect the Ethernet port 1 of the Data Acquisition Unit to an Ethernet port running a DHCP service using a single copper Ethernet cable. For fibre optic
   installations, refer to Promotion User Manual.

3. Attach PCIe cables
   Connect the Sequencing and Data Acquisition units using the supplied PCIe cables (x2 for P24; x3 for P48) 2 according to the port-to-port colour coding shown in the diagram ( ).

4. Attach USB cables
   Connect x1 USB-B 3 and x2 (P24) or x3 (P48) Mini USB 4 cables to the Sequencing Unit. Connect the other end of the cables to the USB-A ports 5 on the Data Acquisition Unit.

5. Attach peripherals
   Connect a monitor to a Mini display port 11 or the VGA port 12 (do not connect both VGA and Mini display at the same time) on the Data Acquisition Unit. Switch
   on the power to the monitor. Connect a mouse and keyboard via the USB inputs 5 on the Data Acquisition Unit.

6. Attach power cables and power on Important: This step must be performed in the order described below.
   a. Attach the power cable to the Sequencing Unit 7 and toggle the power switch at the rear of the unit 8 .
   b. Wait 3 minutes.
   c. Connect the two power cables to the Data Acquisition Unit 7 . This unit will power on automatically once the power cables are connected. Alternatively, the Data Acquisition Unit can be turned on with the power switch 9 .

2. Log in and activate your device

1. Log in to your Promotion Password: prom
2. Open Minnow™ Click the wheel icon on the desktop and log in using your Nanopore Community account
3. Activate your device following the on-screen instructions

Tip: View the pop-up tutorials to learn how to navigate the user interface.

3 Perform hardware check

To ensure the device is working as expected, load your Configuration Test Cells (CTCs) and follow the workflow below to initiate the hardware check. On- screen instructions will guide you through the process.

Hardware check overview:

1. Load the CTCs using the instructions in the User Manual
2. Wait until the flow cell positions initialise with a green light and for the flow cell positions in Min KNOW to turn white
3. Press Select all available to darken the flow cell positions in Min KNOW
4. Press Start in the bottom right
5. Check the flow cell positions show a to pass the hardware check

For more information, refer to the User Manual (community.nanoporetech.com/to/promethion)

4 Check for updates

New software updates will be displayed automatically when opening Min KNOW. You can also manually check and install updates following the workflow below.

5 Power down the device

Promotion must be shut down according to the steps below to prevent potential device errors. Please place the Powering on and off your Promotion 24/48 (A100) flyer that accompanies this Quick start guide next to your Promotion device to support other users.

1. Ensure that no experiments are running and remove all flow cells or Configuration Test Cells (CTCs).
2. Select Host settings in the side bar, then click the Shutdown button in the main window. Confirm shutdown in the pop-up box.
3. Important: Only turn off the Sequencing Unit (using the power switch on the back of the device) once the Data Acquisition Unit has fully shut down.
4. Turn off both devices at the main power supply.

6 Discover the Nanopore Community

https://qrco.de/bbTj9w

community.nanoporetech.com

Ensure the success of your nanopore sequencing project and stay up-to-date with the latest technology and protocol updates.
Use the web browser on your Promotion to navigate to the Nanopore Community.

Additional information

community.nanoporetech.com/to/warranty
community.nanoporetech.com/support/returns
store.nanoporetech.com
community.nanoporetech.com/docs
support@nanoporetech.com community.nanoporetech.com/support

Oxford Nanopore Technologies
phone +44 (0)845 034 7900
email support@nanoporetech.com
twitter @nanopore

www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, Min KNOW, and Promotion are registered trademarks of Oxford Nanopore Technologies plc in various countries. All other brands and names contained are the property of their respective owners. © 2022 Oxford Nanopore Technologies plc. All rights reserved. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition. ONT-08-00749-00-2. BR_1100(EN)_V3_01May2022

Place these instructions next to your Promotion device.

It is important to power on and off the Promotion device according to the instructions below to minimise the potential for device error.

Powering on

1. Ensure there are no flow cells or Configuration Test Cells (CTCs) in the Sequencing Unit.
2. Press the power switch at the back of the Sequencing Unit and wait for 3 minutes.
3. Press the power button on the top of the Data Acquisition Unit.

Powering off

1. Ensure that no experiments are running An indication that no experiments are running is the lack of an Experiment status bar underneath the flow cell on the Sequencing Overview page in the Min KNOW™ user interface. All flow cells or Configuration Test Cells (CTCs) should be removed from the device before powering off.
2. Select Host settings in the side bar, then click the Shutdown button in the main window. Confirm shutdown in the pop-up box.
3. Important: Only turn off the Sequencing Unit (using the power switch on the back of the device) once the Data Acquisition Unit has fully shut down.
4. Turn off both devices at the main power supply.

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