NANOPORE PromethION Data Acquisition Unit User Guide
- September 14, 2024
- NANOPORE
Table of Contents
PromethION Data Acquisition Unit
“`html
Specifications:
- Model: PromethION 24/48 A-Series
- Manufacturer: Oxford Nanopore Technologies
- Contact: Phone +44 (0)845 034 7900, Email
support@nanoporetech.com
Product Usage Instructions:
Powering On:
-
Ensure no flow cells or Configuration Test Cells (CTCs) are in
the Sequencing Unit. -
Press the power switch at the back of the Sequencing Unit and
wait for 3 minutes. -
Press the power button on the top of the Data Acquisition
Unit.
Powering Off:
-
Ensure no experiments are running.
-
Select Host settings in the side bar of the MinKNOWTM user
interface. -
Click the Shutdown button in the main window and confirm
shutdown in the pop-up box. -
Turn off both devices at the main power supply.
-
Note: Only turn off the Sequencing Unit after the Data
Acquisition Unit has fully shut down.
FAQ:
Q: What should I do if experiments are running when I need to
power off the device?
A: Ensure to stop all experiments and confirm no activities are
ongoing before proceeding with the power-off sequence.
Q: Can I use power cables interchangeably between the
Sequencing Unit and Data Acquisition Unit?
A: No, please use the power cables that came specifically with
each unit to avoid any compatibility issues.
“`
Powering on and off your PromethION 24/48 A-Series
Place these instructions next to your PromethION device.
!
It is important to power on and off the PromethION device according to the instructions below to minimise
the potential for device error.
Powering on
Ensure there are no flow cells or Configuration Test Cells (CTCs) in the
Sequencing Unit.
Press the power switch at the back of the Sequencing Unit and wait for 3
minutes.
Press the power button on the top of the Data Acquisition Unit.
Powering off
Ensure that no experiments are running An indication that no experiments are
running is the lack of an Experiment status bar underneath the flow cell on
the Sequencing Overview page in the MinKNOWTM user interface. All flow cells
or Configuration Test Cells (CTCs) should be removed from the device before
powering off.
Select Host settings in the side bar, then click the Shutdown button in the
main window. Confirm shutdown in the pop-up box.
Important: Only turn off the Sequencing Unit (using the power switch on the
back of the device) once the Data Acquisition Unit has fully shut down.
Turn off both devices at the main power supply.
Oxford Nanopore Technologies phone +44 (0)845 034 7900 email support@nanoporetech.com twitter @nanopore
A-Series Data Acquisition Unit
Device connections
9
1. 10G Ethernet ports
7
2. PCIe cable ports
3. USB-B port
4. Mini USB ports
6
5
5. USB-A ports
1
6. USB-C port
12
7. Power sockets
8. Power on/off
2
11
– Sequencing Unit
10
9. Power on/off
– Data Acquisition Unit
10. Fibre Optic SFP Connector
11. Mini display ports
12. VGA port
Sequencing Unit
78
2
Sequencing Unit
78
2
3
4
3
4
www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, MinKNOW, and PromethION are
registered trademarks of Oxford Nanopore Technologies plc in various
countries. All other brands and names contained are the property of their
respective owners. © 2023 Oxford Nanopore Technologies plc. All rights
reserved. Oxford Nanopore Technologies products are not intended for use for
health assessment or to diagnose, treat, mitigate, cure, or prevent any
disease or condition. ONT-08-00762-00-3. FL_1101(EN)_V3_08AUG2023
A-Series Data Acquisition Unit Quick start guide
This Quick start guide contains everything you need to set up your
PromethIONTM 24/48 A-Series and to check the device is ready for use.
For detailed information and troubleshooting, view the User Manual.
Pre-installation
Before using the device, familiarise yourself with the following
PromethION 24/48 A-Series User Manual community.nanoporetech.com/to/promethion
Safety and regulatory information community.nanoporetech.com/to/safety
What’s in the box:*
PromethION Sequencing Unit
PromethION Data Acquisition Unit
Configuration Test Cells
(P24 x24 | P48 x48)
Power cable** (x3)
USB-A/USB-B cable (x1)
Mini DP/ Display port
cable (x1)
USB-A/ Mini USB cables (P24 x2 | P48 x3)
PCIe cables (P24 x2 | P48 x3)
USB-A Stick (ISO reset image)
USB-C/USB-A adapter
- Peripherals not supplied: monitor (Display port or VGA compatible), USB keyboard, USB mouse, Ethernet cable.
** The Data Acquisition Unit ships with 10 power cables (2 x US, 2 x UK, 2 x EU, 2 x CN, 2 x AUS), the Sequencing Unit ships with 5 power cables (1 x US, 1 x UK, 1 x EU, 1 x CN, 1 x AUS). Only those shipped with each unit must be used with that unit.
Set up and power on your device
A-Series Data Acquisition Unit
Device connections
9
1. 10G Ethernet ports
7
2. PCIe cable ports
3. USB-B port
4. USB-Mini ports
6
5
5. USB-A ports
1
6. USB-C port
12
7. Power sockets
8. Power on/off
2
11
– Sequencing Unit
9. Power on/off
10
– Data Acquisition Unit
10. Fibre module cages
11. Mini display ports
12. VGA port
Sequencing Unit
78
2
Sequencing Unit
78
2
3
4
3
4
1. Unbox your PromethION Place the Sequencing Unit and Data Acquisition Unit on a bench less than 2 m from each other. Ensure at least 30 cm clearance around both the Sequencing Unit and the upright Data Acquisition Unit. The devices must be placed away from direct sources of heat or sunlight. Without sufficient airflow, the devices will encounter problems with temperature regulation, which will have a detrimental effect on performance.
2. Attach network cables Connect the bottom Ethernet port 1 of the Data Acquisition Unit to an Ethernet port running a DHCP service using a single copper Ethernet cable. For fibre optic installations, refer to the PromethION User Manual.
3. Attach PCIe cables
Connect the Sequencing and Data Acquisition units using the supplied PCIe
cables (x2 for P24; x3 for P48) 2 according to the port-to-port colour coding
shown in the diagram (
).
4. Attach USB cables Connect x1 USB-B 3 and x2 (P24) or x3 (P48) Mini USB 4 cables to the Sequencing Unit. Connect the other end of the cables to the USB-A ports 5 on the Data Acquisition Unit.
5. Attach peripherals Connect a monitor to a Mini display port 11 or the VGA port 12 (do not connect both VGA and Mini display at the same time) on the Data Acquisition Unit. Switch on the power to the monitor. Connect a mouse and keyboard via the USB inputs 5 on the Data Acquisition Unit.
6. Attach power cables and power on Important: This step must be performed in the order described below, and use the power cables with the Data Acquisition Unit for the Data Acquisition Unit and the power cables with the Sequencing Unit for the Sequencing Unit.
a. Attach the power cable to the Sequencing Unit 7 , turn on the power at the
mains socket and then toggle the power switch at the rear of the unit 8 .
b. Wait 3 minutes.
c. Connect the two power cables to the Data Acquisition Unit 7 and turn the
mains power on. Press the power button 9 to turn on the Data Acquisition Unit.
The boot screen will appear on the monitor.
Log in and activate your device
1. Log in to your PromethION Password: prom.
2. Open MinKNOWTM Click the wheel icon on the desktop and log in using your
Nanopore Community account.
Log in with your Nanopore account Continue as guest
Tip: View the pop-up tutorials to learn how to navigate the user interface.
Check for updates
New software updates will be displayed automatically when opening MinKNOW. You
can also manually check and install updates following the workflow below.
Host settings (in side bar)
Software (in side bar)
Install update (in the MinKNOW panel)
Perform hardware check
To ensure the device is working as expected, insert all the Configuration Test
Cells (CTCs) into the device and follow the workflow below to initiate the
hardware check. On-screen instructions will guide you through the process.
My device
Start (in side bar)
Hardware check (in main panel)
Hardware check overview:
1. Insert the CTCs into the device using the instructions in the User Manual.
2. Wait until all of the LEDs on the device light up green. Then, in the
MinKNOW software, the flow cell status indicators (the 24 or 48 boxes) will
change colour from grey to white.
3. Press Select all available. This will change the colour of the flow cell
status indicators (the 24 or 48 boxes) on the MinKNOW Hardware check panel to
dark blue.
4. Press Start in the bottom right.
5. Check the flow cell positions show a to pass the hardware check.
For more information, refer to the User Manual (community.nanoporetech.com/to/promethion)
Power down the device
The PromethION must be shut down according to the steps below to prevent
potential device errors. Please place the Powering on and off your PromethION
24/48 A-Series flyer that accompanies this Quick start guide next to your
PromethION device to support other users.
1. Ensure that no experiments are running and remove all flow cells or
Configuration Test Cells (CTCs).
2. Select Host settings in the side bar, then click the Shutdown button in
the main window. Confirm shutdown in the pop-up box.
3. Important: Only turn off the Sequencing Unit (using the power switch on
the back of the device) once the Data Acquisition Unit has fully shut down.
4. Turn off both devices at the main power supply.
Discover the Nanopore Community
community.nanoporetech.com
Ensure the success of your nanopore sequencing project and stay up-to-date
with the latest technology and protocol updates.
Use the web browser on your PromethION to navigate to the Nanopore Community.
Training Protocols Forums Support
FAQs
Additional information
Storage and warranty PromethION 24/48 A-Series: Do not expose device to
temperatures outside of 540ºC. For flow cell warranty information, see
community.nanoporetech.com/to/warranty
Recycle used flow cells Oxford Nanopore is committed to environmental
sustainability. Please help us by sending back your PromethION Flow Cells for
recycling. Find out how: community.nanoporetech.com/support/returns
Place your next order Order more PromethION consumables at the Oxford Nanopore
store: store.nanoporetech.com
Documentation PromethION 24/48 A-Series IT requirements, User Manual, and an
online version of this Quick start guide are available at:
community.nanoporetech.com/docs
Support Our technical specialists will be in contact with you shortly to
ensure the device is set up correctly. If you encounter issues at any point,
please contact support@ nanoporetech.com or visit
community.nanoporetech.com/support
Oxford Nanopore Technologies phone +44 (0)845 034 7900 email
support@nanoporetech.com twitter @nanopore
www.nanoporetech.com
Oxford Nanopore Technologies, the Wheel icon, MinKNOW, and PromethION are
registered trademarks of Oxford Nanopore Technologies plc in various
countries. All other brands and names contained are the property of their
respective owners. © 2023 Oxford Nanopore Technologies plc. All rights
reserved. Oxford Nanopore Technologies products are not intended for use for
health assessment or to diagnose, treat, mitigate, cure, or prevent any
disease or condition. ONT-08-00749-00-3. BR_1100(EN)_V3_08AUG2023
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>