Co-Dx ZDC-K-001 Logix Smart Zika, Dengue and Chikungunya IVD Product User Guide
- June 3, 2024
- Co-Dx
Table of Contents
Co-Dx ZDC-K-001 Logix Smart Zika, Dengue and Chikungunya IVD Product
Plate the Master Mix
-
Load the Co-Dx Box software.
-
In the Clean area, clean work surfaces with water > 10% bleach > water > 70% alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids.
-
Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice.
-
Briefly vortex and centrifuge the MM and NFW reagents.
-
Add 5 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample).
-
Pipet 5 µL of NFW into only the NC well and cap the NC well.
-
Place reagents back into the freezer.
Plate Samples and Positive Control (PC)
-
Load the Co-Dx Box software.
-
In the Positive area, clean work surfaces with water > 10% bleach > water > and 70% alcohol (in that order).
-
Thaw the PC on ice.
-
Briefly vortex and centrifuge all reagents.
-
Perform sample extraction, either using RNA extraction or lysis.
-
Add 5 µL of the sample extract to the appropriate wells and cap the sample extract wells.
-
Add 5 µL PC to the PC well and cap the PC well.
-
Place capped tubes in the thermocycler, in appropriate locations and fill the rest of the carousel with blank tubes.
-
(Optional): Cap all tubes and place samples and reagents back into the freezer.
Start the Run
-
In the Co-Dx Box software, click New.
-
Select an assay template and click OK.
-
Under Run Setup, select Samples and type sample information in the appropriate order.
-
Click Information and in the Notes field, type any needed information.
-
Select the appropriate thermocycler and from the drop-down select Start Run.
-
Verify the tube clamp is in place and the appropriate reaction volume is listed, and select Start.
The run initializes and a progress bar gives an estimated time to completion.