Co-Dx VZDC-R-001 Vector Smart Zika Dengue and Chikungunya RUO User Guide

June 3, 2024
Co-Dx

Co-Dx VZDC-R-001 Vector Smart Zika Dengue and Chikungunya RUO

Plate the Master Mix

  • Load the Co-Dx Box software.
  • In the Clean area, clean work surfaces with water > 10% bleach > water > 70%alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids.
  • Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice.
  • Briefly vortex and centrifuge the MM and NFW reagents.
  • Add 5 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample).
  • Pipet 5 µL of NFW into only the NC well and cap the NC well. Place reagents back into the freezer.

Plate Samples and Positive Control (PC)

  • Load the Co-Dx Box software.

  • In the Positive area, clean work surfaces with water > 10% bleach > water > and 70% alcohol (in that order).

  • Thaw the PC on ice.

  • Briefly vortex and centrifuge all reagents.

  • Perform sample extraction, either using RNA extraction or lysis.

  • Add 5 µL of the sample extract to the appropriate wells and cap the sample extract wells.

  • Add 5 µL PC to the PC well and cap the PC well.

  • Place capped tubes in the thermocycler, in appropriate locations and fill the rest of the carousel with blank tubes.

  • (Optional): Cap all tubes and place sam-ples and reagents back into the freezer.

Start the Run

  • In the Co-Dx Box software, click New. Select an assay template and click OK.
  • Under Run Setup, select Samples and type sample information in the appropriate order.
  • Click Information and in the Notes field, type any needed information.
  • Select the appropriate thermocycler and from the drop-down select Start Run.
  • Verify the tube clamp is in place and the appropriate reaction volume is listed, and select Start.

The run initializes and a progress bar gives an estimated time to completion.

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>

Related Manuals