Co-Dx VZDC-R-001 Vector Smart Zika Dengue and Chikungunya RUO User Guide
- June 3, 2024
- Co-Dx
Table of Contents
Co-Dx VZDC-R-001 Vector Smart Zika Dengue and Chikungunya RUO
Plate the Master Mix
- Load the Co-Dx Box software.
- In the Clean area, clean work surfaces with water > 10% bleach > water > 70%alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids.
- Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice.
- Briefly vortex and centrifuge the MM and NFW reagents.
- Add 5 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample).
- Pipet 5 µL of NFW into only the NC well and cap the NC well. Place reagents back into the freezer.
Plate Samples and Positive Control (PC)
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Load the Co-Dx Box software.
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In the Positive area, clean work surfaces with water > 10% bleach > water > and 70% alcohol (in that order).
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Thaw the PC on ice.
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Briefly vortex and centrifuge all reagents.
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Perform sample extraction, either using RNA extraction or lysis.
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Add 5 µL of the sample extract to the appropriate wells and cap the sample extract wells.
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Add 5 µL PC to the PC well and cap the PC well.
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Place capped tubes in the thermocycler, in appropriate locations and fill the rest of the carousel with blank tubes.
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(Optional): Cap all tubes and place sam-ples and reagents back into the freezer.
Start the Run
- In the Co-Dx Box software, click New. Select an assay template and click OK.
- Under Run Setup, select Samples and type sample information in the appropriate order.
- Click Information and in the Notes field, type any needed information.
- Select the appropriate thermocycler and from the drop-down select Start Run.
- Verify the tube clamp is in place and the appropriate reaction volume is listed, and select Start.
The run initializes and a progress bar gives an estimated time to completion.
Read User Manual Online (PDF format)
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