Co-Dx MPXA-R-001 Logix Smart Monkeypox RUO Product User Guide
- June 3, 2024
- Co-Dx
Table of Contents
Quick Start Guide for Using the
Logix Smart™ Monkeypox RUO Product
(MPXA-R-001)
Plate the Master Mix
Perform in Clean Area
a. Load the Co-Dx Box software.
b. In the Clean area, clean work surfaces with water > 10% bleach > water
70% alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids. c. Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice. d. Briefly vortex and centrifuge the MM and NFW reagents. e. Add 5 or 10 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample). f. Pipet 5 µL of NFW into only the NC well and cap the NC well. g. Place reagents back into the freezer.
Plate Samples and Positive Control (PC)
Perform in Positive Area
a. Load the Co-Dx Box software.
b. In the Positive area, clean work surfaces with water > 10% bleach >
water > and 70% alcohol (in that order). c. Thaw the PC on ice. d.
Briefly vortex and centrifuge all reagents.
e. Perform sample extraction, either using RNA extraction or lysis.
f. Add 5 or 10 µL of the sample extract to the appropriate wells and cap
the sample extract wells.
g. Add 5 or 10 µL PC to the PC well and cap the PC well.
h. Place capped tubes in the thermocycler, in appropriate locations and fill
the rest of the carousel with blank tubes.
i. (Optional): Cap all tubes and place samples and reagents back into the
freezer.
Start the Run
Perform in Co-Dx Box Software
a. In the Co-Dx Box software, click New. b. Select an assay
template and click OK c. Under Run Setup, select Samples and type sample
information in the appropriate order.
d. Click Information and in the Notes field, type any needed information.
e. Select the appropriate thermocycler and from the drop-down select Start
Run. f. Verify the tube clamp is in place and the appropriate reaction
volume is listed, and select Start. The run initializes and a progress bar
gives an estimated time to completion.
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