dynamic BIOSENSORS AS-1-Rb v5.1 Adapter Strand User Manual
- August 28, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-1-Rb v5.1 Adapter Strand
Specifications
- Product Name: heliX+ ADAPTER STRAND 1 with red dye Rb
- Manufacturer: Dynamic Biosensors GmbH & Inc.
- Order Number: AS-1-Rb
- Key Features: 2 spots with 2 different anchor sequences for DNA-encoded addressing
- Concentration: 400 nM
- Storage: Black cap, limited shelf life, avoid freeze-thaw cycles, aliquot the nanolever
Product Usage Instructions
Preparation | MIX &RUN
- Mix Adapter strand 1 – Rb (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution from step 1 at room temperature at 600 rpm for 30 minutes for complete hybridization.
- Mix the solution from step 2 with Adapter strand 1 – Rb – lfs (200 nM) at 1:1 ratio (v/v).
- The solution is now ready for biochip functionalization.
Note: Stability of the solution is dependent on the stability of the ligand molecule.
FAQs
-
Q: What is the concentration of Adapter strand 1 – Rb – lfs?
A: The concentration is 200/250 nM as per the product description. -
Q: Can Adapter strand 2 be used interchangeably with Adapter strand 1?
A: No, Adapter strand 2 with red dye Rb is prehybridized with ligand-free strand and has a different order number (HK-NHS-1 AS-2-Rb-lfs).
heliX+
User Manual
ADAPTER STRAND 1
with red dye Rb
Dynamic Biosensors GmbH & Inc.
AS-1-Rb v5.1
Key Features
- Adapter strand 1 – Rb for functionalization of heliX® Adapter Chip Spot 1.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 1 carries a moderately hydrophilic red dye (Rb) with a neutral net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-1-Rb
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Adapter strand 1 – Rb | Black | 400 nM | 5 x 100 µL | TE40 [ 1] |
-20°C
For research use only.
- This product has a limited shelf life, please see expiry date on label.
- To avoid many freeze thaw cycles please aliquot the nanolever
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Rb (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 1 – Rb – lfs (200 nM) at 1:1 ratio (v/v). Solution is ready to use for biochip functionalization. Stability of the solution is related to the stability of the ligand molecule
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
|---|
Ligand strand
carrying the conjugated ligand
| 500 nM| P 2 ] E40 [| heli X ® Amine Coupling Kit 1| HK-NHS-1
Adapter strand 1 – Rb – lfs| 200/250 nM| TE40 [ 1 ]| Adapter
strand 2 with red dye Rb prehybridized with ligand-free strand| AS-2
-Rb-lfs
Example
Required volume for 3 functionalizations: 100 μL with a final concentration of
100 nM.
| Vial 1 | Vial 2 |
|---|
Adapter strand 1 – Rb
(400 nM)
| Conjugated Ligand strand (500 nM)| Adapter strand 1 – Rb – lfs
(200/250 nM)
25 µL| 25 µL| 50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
- Perchtinger Str. 8/10
- 81379 Munich
- Germany
Dynamic Biosensors, Inc.
- 300 Trade Center, Suite 1400
- Woburn, MA 01801
- USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2024
Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
- TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
- If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
References
Read User Manual Online (PDF format)
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