dynamic BIOSENSORS HK-SA-1 v6.1 Biotin Capture Kit User Manual
- August 28, 2024
- dynamic BIOSENSORS
Table of Contents
- dynamic BIOSENSORS HK-SA-1 v6.1 Biotin Capture Kit
- Product Specifications
- Product Description
- Contact Information
- Useful Order Numbers
- FAQ
- Key Features
- heliX® Adapter Chip Overview
- Product Description
- Workflow of a heliX® SA – capture assay
- Preparation
- Assay Setup in heliOS
- Useful Order Numbers
- Contact
- References
- Read User Manual Online (PDF format)
- Download This Manual (PDF format)
dynamic BIOSENSORS HK-SA-1 v6.1 Biotin Capture Kit
Product Specifications
- Product Name: heliX+ BIOTIN CAPTURE KIT with red dye Ra
- Order Number: HK-SA-1
- Key Features:
- 2 spots with 2 different anchor sequences for DNA-encoded addressing
- Spot 1 functionalized with the capture molecule
- Spot 2 used as real-time reference
Product Description
This product is designed for research use only. It consists of ligand strands, adapter strands, and a capture molecule with specific concentrations and storage requirements. The product has a limited shelf life and should be used within 3 months after preparation of the solution.
Preparation
To prepare for assay setup in heliOS, follow these steps:
- Go to heliOS and create a New Assay Workflow
- Add Custom Assay and load Capture with Kinetics
- Modify the parameters based on your needs and run the assay
Assay Setup in heliOS
Suggested assay parameters such as flow rate, time, and LED power are
within the heliOS assay. Streptavidin can bind multiple ligands per nanolever,
leading to avidity effects when measuring multispecific analytes. Consider
using the conjugation approach in such cases.
Contact Information
- For further questions or technical support, please contact Dynamic Biosensors at:
- Email: support@dynamic-biosensors.com
- Website: www.dynamic-biosensors.com
- Address:
- Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany
- Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA
Useful Order Numbers
- Chip with 2 detection spots: Order No ADP-48-2-0
- For passivation of chip surface: Order No SOL-PAS-1-5
- For regeneration of chip surface: Order No SOL-REG-1-5
FAQ
-
What is the shelf life of the product?
The product has a limited shelf life, and it should be used within 3 months after preparation of the ready-to-use solution. -
How can I regenerate the chip surface?
Surface regeneration can be done by injecting a high pH solution. This process restores the chip surface to its original state.
Key Features
- This kit is designed for capture of biotinylated molecules using Streptavidin.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands and Ligand strand modified with Streptavidin for 20 regenerations.
- For functionalization of Spot 1 and Spot 2.
- Adapter strands 1 and 2 carry a moderately hydrophilic red dye (Ra) with a single positive net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing. Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference.
Product Description
Order Number: HK-SA-1
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| SA – Ligand strand | Green | 500 nM | 2 x 100 µL | TE40 [ 1] | 2-8°C |
| Adapter strand 1 – Ra | Black | 400 nM | 2 x 100 µL | TE40 [ 1] |
-20°C
Adapter strand 2 – Ra – lfs| White| 200/250 nM| 2 x 200 µL| TE40 [
1]| -20°C
For research use only.
This product has a limited shelf life, please see expiry date on label.
After preparation of ready to use solution the expiry date is 3 months.
Workflow of a heliX® SA – capture assay
- The anchor strand (ssDNA) immobilized to the surface of the heliX® Adapter Chip is hybridized with complementary DNA strands modified with Streptavidin.
- The biotinylated ligand of interest is captured on the surface during the measurement run.
- Measurement of the analyte binding kinetics or conformational change upon analyte binding.
- Surface regeneration by injection of a high pH solution. Chip surface goes back to the original state. This step can be followed by a new hyridization of fresh ligand with Streptavidin.
Preparation
- Mix 100 μL SA – Ligand strand with 100 μL Adapter strand 1 – Ra.
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix 200 μL Adapter strand 2 – Ra – lfs to the sample after step 2.
- The solution (400 μL in total) is ready to use for a biochip functionalization.
- Please aliquot and store the ready to use solution at 2-8°C. Use up within 3 months.
- The kit contains material for the preparation of two separate ready to use solutions with 400 μL each.
Assay Setup in heliOS
-
Go to heliOS > create a New Assay Workflow > add Custom Assay > load Capture with Kinetics > modify the parameters based on your needs and run the assay.
-
Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.
TIP As streptavidin owns four different binding sites, multiple ligands can be captured per nanolever, leading to avidity effects when measuring multispecific analytes. If the case, consider using the conjugation approach. -
For further questions, please contact the support team at support@dynamic-biosensors.com.
Useful Order Numbers
| Product Name | Comment | Order No |
|---|---|---|
| heli X ® Adapter Chip | Chip with 2 detection spots | ADP-48-2-0 |
| 10x Passivation solution | For passivation of chip surface | SOL- |
PAS-1-5
Regeneration solution| For regeneration of chip surface| SOL-REG-1-5
Contact
-
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany -
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA -
Order Information order@dynamic-biosensors.com
-
Technical Support support@dynamic-biosensors.com
-
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>