dynamic BIOSENSORS HK-NTA-1 v4.1 HIS Capture Kit User Manual
- August 28, 2024
- dynamic BIOSENSORS
Table of Contents
- dynamic BIOSENSORS HK-NTA-1 v4.1 HIS Capture Kit
- Product Specifications
- Product Description
- Contact Information
- FAQ
- Key Features
- heliX® Adapter Chip Overview
- Product Description
- Workflow of a heliX® His – capture assay
- Preparation
- Assay Setup in heliOS
- Useful Order Numbers
- Contact
- References
- Read User Manual Online (PDF format)
- Download This Manual (PDF format)
dynamic BIOSENSORS HK-NTA-1 v4.1 HIS Capture Kit
Product Specifications
- Product Name: heliX+ HIS CAPTURE KIT with red dye Ra
- Order Number: HK-NTA-1
- Key Features:
- 2 spots with 2 different anchor sequences for DNA-encoded addressing
- Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference
Product Description
Material| NTA – Ligand strand| Adapter strand 1 – Ra| Adapter strand 2 – Ra|
Loading Solution (NiCl2)| Imidazole Solution
---|---|---|---|---|---
Color| Red| Black| White| Transparent| Transparent
Concentration| 500 nM| 400 nM| 200/250 nM| 10 mM| 250 mM
Buffer: TE40 [1] For research use only. This product has a limited shelf life, please see expiry date on label. After preparation of ready to use solution, the expiry date is 6 months.
Preparation:
- Assay Setup in heliOS: Go to heliOS > create a New Assay Workflow > add Custom Assay > load His Capture with Kinetics > modify the parameters based on your needs and run the assay.
- Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.
- If the stability of his capture is affected by the protein, in case of long dissociations, consider using the conjugation approach.
Contact Information
-
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich Germany -
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801 USA -
Order Information:order@dynamic-biosensors.com
Technical Support:support@dynamic-biosensors.com
FAQ
-
What is the shelf life of the product?
The shelf life of the product is limited. Please check the expiry date on the label. After preparing the ready-to-use solution, the expiry date is 6 months. -
How can I regenerate the chip surface?
The chip surface can be regenerated either by injection of a high pH solution followed by a new hybridization of fresh DNA-NTA or by simple removal of the his-tagged protein via imidazole wash.
Key Features
- This kit is designed for capture of histidine-tagged proteins (His6 or His10) using Tris-NTA.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands and Ligand strand modified with Tris-NTA for 20 regenerations.
- For functionalization of Spot 1 and Spot 2.
- Adapter strands 1 and 2 carry a moderately hydrophilic red dye (Ra) with a single positive net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing. Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference.
Product Description
Order Number: HK-NTA-1
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| NTA – Ligand strand | Red | 500 nM | 2 x 100 µL | TE40 [ 1] |
-20°C
Adapter strand 1 – Ra| Black| 400 nM| 2 x 100 µL| TE40 [ 1]|
-20°C
Adapter strand 2 – Ra – lfs| White| 200/250 nM| 2 x 200 µL| TE40 [
1]| -20°C
Loading Solution (NiCl 2)| Transparent| 10 mM| 5 x 1500 µL| TE40
[ 1]| -20°C
Imidazole Solution| Transparent| 250 mM| 10 x 2000 µL| T 2]E140 [|
-20°C
- For research use only.
- This product has a limited shelf life, please see expiry date on label.
- After preparation of ready to use solution the expiry date is 6 months.
Workflow of a heliX® His – capture assay
- The anchor strand (ssDNA) immobilized to the surface of the heliX® Adapter Chip is hybridized with complementary DNA strands modified with Tris-NTA (Nitrilotriacetate).
- NTA-DNA surface is activated by loading with divalent nickel ions. His-tagged protein (ligand) is captured on the surface.
- Measurement of the analyte binding kinetics or conformational change upon analyte binding.
- Surface regeneration either by injection of a high pH solution followed by a new hybridization of fresh DNA-NTA or by simple removal of the his-tagged protein via imidazole wash.
Preparation
- Mix 100 µL NTA – Ligand strand with 100 µL Adapter strand 1 – Ra.
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix 200 µL Adapter strand 2 – Ra – lfs to the sample after step 2.
- The solution (400 µL in total) is ready to use for a biochip functionalization.
- Please aliquot and store the ready to use solution at -20°C. Use up within 6 months.
- The kit contains material for the preparation of two separate ready to use solutions with 400 µL each.
Assay Setup in heliOS
-
Go to heliOS > create a New Assay Workflow > add Custom Assay > load His Capture with Kinetics > modify the parameters based on your needs and run the assay.
-
Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.
TIP As the stability of his capture is affected by the protein, in case of long dissociations, consider using the conjugation approach. -
For further questions, please contact the support team at support@dynamic-biosensors.com.
Useful Order Numbers
| Product Name | Comment | Order No |
|---|---|---|
| heli X ® Adapter Chip | Chip with 2 detection spots | ADP-48-2-0 |
| 10x Passivation solution | For passivation of chip surface | SOL- |
PAS-1-5
Regeneration solution| For regeneration of chip surface| SOL-REG-1-5
Contact
-
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany -
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA -
Order Information order@dynamic-biosensors.com
-
Technical Support support@dynamic-biosensors.com
-
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>