BRESSER 5750600 Trinocular Transmitted Light Microcope Instruction Manual

BRESSER 5750600 Trinocular Transmitted Light Microcope Instruction Manual

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Bresser GmbH, Gutenbergstr. 2, 46414 Rheed, Germany www.bresser.de

In the event of any warranty claims or service enquiries, please refer to the “Warranty” and “Service” sections of these operating instructions. We ask for your understanding that unsolicited returns cannot be processed. Errors and technical changes reserved.
© 2024 Bresser GmbH. All rights reserved.

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VALIDITY NOTE
This instruction manual is valid for products with the following article numbers: 5750600
MANUAL VERSION: 0524
MANUAL NAME:
Manual_5750600_Bioscience-Trino_de_BRESSER_v052024a
If you have any queries, please quote this information.

ABOUT THIS INSTRUCTION MANUAL

NOTE:
THESE INSTRUCTION MANUAL ARE TO BE REGARDED AS PART OF THE DEVICE.
Read the safety instructions and the instruction manual carefully before using this device. Keep these instruction manual in a safe place for future reference. If the device is sold or passed on, the instruction manual must be handed over to the new owner/user of the product.

GENERAL WARNINGS

DANGER OF AN ELECTRIC SHOCK!
This device contains electronic parts that are powered by a power source (mains connection). Never leave children unattended when handling the device! Only use the device as described in the instruction manual, otherwise there is a RISK of an ELECTRIC SHOCK!

• Disconnect the power supply when you are not using the device, during long breaks, and before all maintenance and cleaning work (disconnect the mains plug).
• Place the device so that it can be disconnected from the power supply at any time. The power outlet should always be near the device and should be easily accessible, as the plug of the power cord serves as a part to disconnect the device from the mains supply.
• To disconnect the device from the mains, always pull the mains plug and never the cable!
• Check the device, cables and connections for damage before use.
• Do not use the device if is damaged or contains damaged live parts! Damaged parts must be replaced  immediately by an authorized service centre.
• Only operate the device in a completely dry environment and do not touch the device with wet or damp body parts.

DANGER OF SUFFOCATION!
Improper use can lead to a RISK OF SUFFOCATION, especially for children! It is therefore imperative that you observe the following safety information.

• Keep packaging materials (plastic bags, rubber bands, etc.) away from children!
• This product contains small parts that can be swallowed by children!
• If small parts are swallowed, seek medical advice immediately!

FIRE/EXPLOSION HAZARD!
Improper use of this product poses a RISK OF FIRE/EXPLOSION! Always observe the following safety instructions to avoid fires and/or explosions.

• Do not expose the device to high temperatures. Use only the supplied AC adapter. Do not short-circuit the device or dispose of it in fire! Excessive heat and improper handling can cause short circuits, fires and even explosions!
• Never cover the ventilation slots or cooling fins of the device during operation or until it has cooled down completely!

RISK OF INJURY!
This device contains components and/or accessories that can cause minor or serious body injury if used improperly. It is therefore essential that you observe the following safety information in order to prevent body injuries.

• This device often requires the use of sharp-edged and pointed tools. For this reason, keep this device together with all accessories and tools in a place inaccessible to children!
• Keep chemicals and liquids out of reach of children! Don’t drink! Clean hands thoroughly under running water after use.
  In case of accidental contact with eyes or mouth, rinse with water. In case of complaints, consult a doctor immediately and show the substances.

THIS DEVICE GENERATES HEAT!
This device contains a halogen light source (12V/30W). Halogen lamps emit a considerable amount of infrared light as heat. As a result, the surface of the lamp housing becomes very hot. Leave sufficient space around the microscope and DO NOT place it near flammable materials. Important: The heat generated can also cause the specimens to warm up! Adjust the brightness to your requirements: Set the Koehler illumination on the microscope by illuminating only the part of the sample that you want to inspect. Switch off the light when you are not using the microscope.

DANGER OF PROPERTY DAMAGE!
Improper handling can cause DAMAGE to the appliance and/or the accessories! Therefore, use the device only in accordance with the following safety information.

• Do not disassemble the device! In case of a defect, contact your dealer. The dealer will contact the service centre and send the device for repair if necessary.
• Do not expose the device to high temperatures and protect it from water and high humidity!
• Do not subject the device to shocks!
• Only use accessories and spare parts that comply with the technical specifications!

SCOPE OF DELIVERY

A: Transmitted light microscope
B: 2x pcs. eyepieces (WF 10x)
C: 4x pcs. objectives (4x, 10x, 40x, 100x oil / pre-assembled)
D: Coaxial cross table with Nonius scale (pre-assembled)
E: 230V power supply unit (12V / 3.3A)
F: Color filter: transparent and blue
G: Immersion oil
H: Dust cover

CHOICE OF LOCATION & SETUP

Before setting up your microscope, choose a suitable location.
Place the microscope on a surface that meets the following criteria:

• Flat/level
• Vibration-free/stable
• Hard, non-combustible surface
• Chemically and mechanically resistant

Set up the microscope so that …

• the ventilation slots are not blocked.
• there is a distance of at least 10 cm around the device or to other appliances or the wall.
• you can disconnect it from the power supply at any time.

ATTENTION! Always use the supplied 230V power supply unit (12V/3.3A) for the halogen illumination (12V/30W) with dimmer. Using an unsuitable power supply unit can cause damage to the microscope!

PRODUCT DESIGN BIOSCIENCE 40-1000X TRINOCULAR MICROSCOPE

FRONT/SIDE VIEW

_ Fig. 1a: Components on the Bioscience 40-1000x trinocular microscope front/side view

_

EQUIPMENT

1. Eyepiece
2. Nosepiece
3. Objective
4. Stage with Nonius scale (cross table)
5. Microscope slide with sample
6. Condenser up-down knob
   • A. Condenser
   • B. Aperture diaphragm lever
   • C. Condenser auxiliary lens
7. Condenser centering screws
8. Condenser holding screw
9. Blue filter (included in the scope of delivery)
   • A. Field diaphragm with filter holder
   • B. Field diaphragm ring
10. Microscope base
11. Storage compartment
12. X-axis knob
13. Y-axis knob
14. Tension adjustment ring
15. Right-hand coarse focusing knob (coarse drive)
16. Right-hand fine focusing knob (fine drive)
17. Carrying handle on the microscope body
18. Microscope head holding screw
19. Trinocular tube
20. Connection of an external camera possible via suitable adapter (not included)
    **BACK VIEW

Fig. 1b:Components on the Bioscience 40-1000x trinocular microscope front/side view.
EQUIPMENT**

21. ON/OFF switch, dimmer
22. mains connection socket
23. Connection cable from the power supply unit (see 5. A)
24. Limit knob (up-stop)
25. Left-hand coarse focusing knob (coarse drive)
26. Left-hand fine focusing knob (fine drive)
27. Holder for the eyepieces

MICROSCOPE SETTINGS

A ESTABLISH MAINS CONNECTION

To start up the microscope, first insert the hollow plug (1) of the supplied power supply unit (12V/3.3A) (2) into the mains connection socket (3) on the back of the microscope. The mains plug (4) of the power supply unit is then plugged into a suitable mains socket (5) (max. 230V).
ADJUST THE LIGHTING
Turn the ON/OFF switch, dimmer (6) down towards the wide bar mark to switch on the lighting.
The dimmer is also used to control the brightness of the halogen illumination (down = brighter, up = darker).
WARNING: Take care not to leave the dimmer at maximum brightness for long periods of time. This shortens the lifespan of the lamp.

B POSITION THE SPECIMEN

The clamp holder (1) on the stage (2) is part of the cross table and designed to hold a microscope slide (3). The Y-axis knob (4) (for movement in the longitudinal direction) and the X-axis knob (5) (for movement in the transverse direction) are coaxial. The stage can therefore be moved according to your requirements.
Swivel the objective (6) (starting with the 4x objective) into the working position by turning the nosepiece (7).
NOTE: The objectives engage with an audible “click” when they are positioned in the beam path.

C ADJUSTING THE OBSERVATION TUBE

Adjusting the interpapillary distance (A) (distance between the eyepieces)
Set the correct interpapillary distance for binocular observation by swiveling the eyepiece tubes (1) on the trinocular microscope head so that the right and left fields of view completely overlap or combine to form a circular image.
Setting the visual acuity correction (diopter compensation)
Turn the 40x objective to the working position. First, look only through the right eyepiece with your right eye, and then use the coarse and fine focusing knobs to focus the image. Next, look only through the left eyepiece with your left eye, and then use the diopter adjustment ring (2) to focus the image.

D FOCUSING THE SPECIMEN

Next, bring the specimen into focus and adjust the image sharpness (focus).
The microscope has a coaxial coarse and fine focusing mechanism. Turn the coarse focusing knob (1) to raise or lower the stage (cross table). Use the fine focusing knob (2) to make sensitive and precise adjustments to the image sharpness. The tension adjustment ring (3) next to the coarse focusing knob (1) is used to correctly adjust the heavy or light movement of the coarse focusing knob so that the stage (cross table) does not slide down by itself. The limit knob (up-stop) (4) prevents unintentional contact between the specimen and objective when focusing. If you lock it at a certain height (by setting it upwards), you will not be able to use the coarse focusing knob to bring the objective closer to the specimen, which is thus protected against damage.
NOTE: The supplied blue filter (5) can be inserted into the filter holder of the field diaphragm. The blue filter makes the light color more neutral.
NOTE: The condenser has an auxiliary lens (6). This lens is used to adjust the condenser aperture to the objective aperture. To observe with the 4x objective, swivel the condenser auxiliary lens into the beam path. This is used to illuminate the field of view. When observing with higher magnification objectives, swivel the condenser auxiliary lens out of the beam path.
NOTE : Always start the observation with the lowest magnification: This makes it easier to center and adjust the specimen and also protects the microscope from damage.
WARNING: The halogen lamp generates a significant amount of heat and will cause the specimens to heat up! Use the ON/OFF switch, dimmer (7) on the back of the microscope to protect the specimens.

E TRINOCULAR TUBE FOR PHOTOGRAPHY

The trinocular tube (1) offers the option of connecting a Micro Cam or C-mount microscope camera. To connect them, you need the C-Mount Mikro Cam adapter (item no: 5942101). An optional adapter (article no:
5942100) for SLR or system cameras is also available. A camera-specific T2 ring is also required here.
The adapter holding screw (2) secures the matching adapter/dust cover.

ILLUMINATION PRINCIPLE ACCORDING TO KOEHLER

This special technology ensures uniformly bright, high-contrast and reflection-free illumination of the sample to be examined. In addition to making full use of the microscope optics, it also protects sensitive samples from excessive light irradiation. Illumination according to Koehler is therefore the standard for scientific and photomicrography applications, and can be adjusted with the built-in field diaphragm together with the height- adjustable and center able Abbe condenser (numerical aperture 1.25; with iris diaphragm).

CENTERING THE CONDENSER

Height-adjustable and center able Abbe condenser
The condenser is moved up or down by turning the condenser up-down knob
The condenser centering screws (2) are used to center the condenser. After loosening the condenser holding screw (3), the condenser (4) can be easily removed from its holder. The field diaphragm with filter holder (5) offers space for a filter disk (blue filter is included in the scope of delivery).
The following section describes step-by-step how to set the illumination according to Koehler using the 10x objective as an example:
a) Using the condenser up-down knob (1), bring the condenser (4) to the highest position directly under the stage.
b) Switch on the illumination with the ON/OFF switch, dimmer (6) – on the back of the microscope – and focus a high-contrast sample with the 10x objective. Make sure that the lighting is set appropriately.

NOTE: Swivel the auxiliary condenser lens (7) out of the beam path when centering with the 10x objective. Otherwise you will not be able to carry out centering.
c) Turn the field diaphragm ring (8) in the direction of the arrow so that the field diaphragm (5) is completely closed. Look through the eyepieces. Adjust the condenser up-down knob (1) until the still blurred image of the field diaphragm is in focus.
d) If the condenser is not centered, you will see an image of the field diaphragm similar to (9A).
e) To center the aperture in the image field, use the two condenser centering screws (2) on each side of the condenser holder.
f) As soon as the image is in focus and centered in the field of view (9B), open the field diaphragm until its edge has just disappeared from the field of view and the field of view is completely illuminated (9C-9D). If needed, you may be able to re-center the condenser a little.
You should also set the aperture diaphragm, which is explained in the next section.

NOTE: Bright-field microscopy is the most common of all light microscopy methods, as it allows high-contrast or stained specimens to be viewed quickly and easily. In order to fully utilize the optical performance of the microscope, especially the objective, the condenser, field diaphragm and aperture diaphragm should be set according to the Koehler illumination principle.
NOTE: The field diaphragm limits the diameter of the light beam entering the objective. This prevents the incidence of stray light and increases the image contrast. To support the performance of the objective, the diameter of the field diaphragm must be adjusted so that its image and the field of view are the same size.

APERTURE DIAPHRAGM (CONDENSER DIAPHRAGM)

The aperture diaphragm (condenser diaphragm) determines the numerical aperture of the illumination system as well as the resolution of the microscope. It is used to set the contrast, image resolution and depth of field. Closing the aperture diaphragm increases the depth of field. The aperture diaphragm (condenser diaphragm) (1) can be opened or closed using the aperture diaphragm lever (2).
a) Remove one eyepiece and look into the open eyepiece tube.
b) Set the aperture diaphragm image (3) by adjusting the aperture diaphragm lever (2) to approx. 70-80% of the diameter of the lens pupil (4).
c) Then insert the eyepiece back into the eyepiece holder.
NOTE: Each time the objective is changed, the object field size and the objective aperture and possibly the centering will change slightly, so that the light field and aperture diaphragm settings should be readjusted for optimum results.
WARNING: The brightness can only be adjusted via the dimmer, and not via the aperture diaphragm.

USE OF OIL IMMERSION

a) Focus the object with the 40x objective (1).
b) Move the Y-axis knob (2) to expose the condenser lens (3). Turn the 40x objective to the side.
c) Apply a drop of immersion oil (4) to the lens of the condenser and lower the condenser slightly.
d) Move the microscope slide (5) into position using the Y-axis knob (2) and lift the condenser until the oil drop get in contact with the slide.
e) Put another drop of immersion oil (4) on the cover glass of the microscope slide and turn the 100x objective (6) into the beam path, so that the front lens is immersing in the oil.
e) Slowly refocus the image using the fine focusing knob (7).
NOTE: Oil immersion can only be performed with a suitable 100x objective. The 100x oil immersion objective is included in the scope of delivery.
NOTE: We recommend immersion oil with a refractive index (RI) of nD=1.515 in accordance with ISO 8036.
NOTE: As air bubbles in the oil impair the image quality, make sure that the oil drop is free of bubbles. To remove bubbles, defocus the oil immersion objective repeatedly and refocus it, or switch to another objective and allow the visible bubbles to burst before immersing the 100x objective again.
NOTE: Clean the 100x objective by dabbing off the oil with a soft baby paper towel (lotion-free). If you use the immersion objective regularly, the objective should only be cleaned with a damp cloth once a week. Use a solution of 70% ethanol or isopropanol (to prepare such a solution, use p.a. or pharmacy alcohol and double-distilled water).

WARNING: If the immersion oil comes into contact with skin, wash it off thoroughly with soap and water. If immersion oil gets into the eyes, rinse them under running water for at least 15 minutes.

REPLACING THE LAMP

a) Turn the ON/OFF switch, dimmer (1) in the “OFF” direction until you hear a click. Then disconnect the mains plug of the power supply unit from the mains socket. Finally, pull the hollow plug (2) out of the mains connection socket (3) on the back of the microscope.
b) Carefully tilt the microscope and loosen the mounting screw (4) on the bottom flap (5), which carries the lamp holder and is located in the middle of the underside of the microscope. Open the bottom flap (5).
c) Pull the old lamp (6) out of the lamp holder ( 7 ).
WARNING: Be careful when doing this, as the lamp may still be hot!
d) Insert a replacement lamp (6) into the lamp holder (7).
NOTE: Make sure that you do not touch the lamp directly with your fingers (to prevent the lamp from becoming dirty, which reduces its service life and intensity). For example, use the lamp`s protective cover or a handkerchief to grip the lamp.
e) Close the bottom flap (5) on the underside again with the mounting screw (4).
f) Once you have fitted the replacement lamp, reconnect the mains connection and switch on the ON/OFF switch, dimmer (1). Then swivel an objective into the beam path, set the condenser to the correct height using the condenser up-down knob (8) and illuminate the field of view. If the light spot is shifted away from the center of the field of view, loosen the other  mounting screw (9) slightly and use it to move the lamp holder (7). Guide the light spot of the lamp into the center, then immediately tighten the mounting screw (9) again

CLEANING/MAINTENANCE/TRANSPORT

During long breaks, and before carrying out any maintenance and cleaning work, disconnect the power supply by
unplugging the mains plug.
Remove all interchangeable optical elements (e.g. objectives, eyepieces, etc.) before cleaning.
The nosepiece, the coarse/fine focusing mechanism and the condenser are precisely designed and calibrated to work
together. Please avoid dismantling the components. This impairs the performance of the microscope.
CLEANING
Lens cleaning:
Wipe the lenses with a lens cleaning cloth or a soft fabric that you have previously moistened with a small amount of 70%vol. alcohol. Clean the 100x immersion objective and the condenser lens each time after the use of immersion oil. Cleaning of microscope and optical elements: Only clean the outside of the microscope with a dry cloth. First, blow loose dust off the lens surfaces.
Use high quality lens cleaning cloths or soft fabric and moisten them lightly with pure alcohol (available in the  pharmacy). Gently wipe the lens surface by cleaning the surface with circular movements from the center to the edge of the lens. Apply slight pressure to the optics.
WARNING: Do not use organic solvents (e.g. alcohol, ether, acetone, xylene or other thinners) to clean painted parts or plastic parts!
WARNING: Alcohol is highly flammable.
WARNING: To avoid damaging the electronics, do not use any cleaning fluid.

Cleaning the 100x oil objective/condenser lens
The immersion oil should be removed from the objective/condenser lens if it has not been used for a long time.
Clean the 100x objective (oil) and the condenser lens
by dabbing off the oil with a soft baby paper towel (lotion free). If you use the 100x lens with oil immersion regularly, cleaning once a week with a solution of 70 % ethanol or isopropanol is sufficient (to prepare such a solution, use p.a. or pharmacy-grade alcohol and double-distilled water).
Dust cover:
When not in use, cover the microscope with the dust cover and place it in a dry and mold-free place. We recommend storing all objectives and eyepieces in a closed container with desiccant.
Transport:
Remove all loose components and the specimen to be observed from the cross table. Please pack the microscope carefully (if possible in the original packaging). The nosepiece, the coarse/fine focusing mechanism and the condenser are precisely designed and calibrated to work together. Take particular care to ensure that they are not damaged during transportation.

TECHNICAL DATA

• Illumination according to Koehler
• External power supply unit for increased safety (230 V power supply unit (12V / 3.3A))
• Stage: coaxial cross table with Nonius scale
• The movement range of the cross table is 50 mm longitudinally and 75 mm transversely
• Coarse and fine focusing
• Fine focusing knob: Smallest scale division: 0.002 mm
• Adjustment range for interpupillary distance: 53 mm – 75 mm
• Trinocular tube offers the option of directly connecting a MicroCam or C-mount eyepiece camera
• 2x eyepieces: both wide field eyepieces 10x, field of view number (FN): 18 mm, plug-in diameter: 23 mm
• 4x objectives: with DIN 4x, 10x, 40x, 100x (oil) magnification, (objective type: achromatic)
• Condenser: Abbe condenser, height-adjustable and centerable (numerical aperture 1.25 with iris diaphragm)
• Illumination: 12V / 30W halogen with dimmer
• Replacement halogen lamp (12V, 30W)

DISPOSAL

Do not dispose of electrical appliances with household waste! In accordance with Directive 2002/96/EC of the European Parliament on waste electrical and electronic equipment and its transposition into German law, used electrical appliances must be collected separately and recycled in an environmentally friendly manner.
Please observe the current legal regulations when disposing of the microscope and all accessories! Information on proper disposal can be obtained from municipal waste disposal service providers or the Environmental Agency.
Dispose of the packaging materials according to type. Information on proper disposal can be obtained from municipal waste disposal service providers or the Environmental Agency.

WARRANTY

The regular warranty period is 2 years and begins on the day of purchase. To benefit from an extended voluntary warranty period as indicated on the gift box, registration on our website is required.
You can view the full warranty conditions and information on warranty extensions and services at www.bresser.de/warranty_terms

www.bresser.de/warranty_terms

EC DECLARATION OF CONFORMITY

Bresser GmbH has prepared a “ CE Declaration of Conformity” in accordance with the applicable guidelines and corresponding standards. The full text of the CE Declaration of Conformity is available at the following website.
www.bresser.de/download/5750600/CE/5760100_CE.p

Please contact the service centre first for any questions regarding the product or claims, preferably by e-mail

e-mail: service@bresseruk.com
*Telephone: +44 1342 837 098
BRESSER UK Ltd**.
Suite 3G, Eden House Enterprise Way Edenbridge, Kent TN8 6HF Great Britain
Number charged at local rates in the UK (the amount you will be charged per phone call will depend on the tariff of your phone provider); calls from abroad will involve higher costs.

Bresser GmbH
Gutenbergstraße 2
46414 Rhede · Germany
www.bresser.de

Bresser UK Ltd.
Suite 3G, Eden House Enterprise Way
Edenbridge, Kent TN8 6HF Great Britain

@BresserEurope

References

• Bresser | Startseite | Expand Your Horizon mit BRESSER

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