euromex Delphi-X Observer Trinocular Microscope User Manual

user manual

Introduction

Thank you for purchasing the Euromex Delphi-X Observer
The Delphi-X Observer series has been designed with all kinds of Life Sciences applications and great durability in mind. This resulted in a modern, robust, and high-level microscope for advanced use, equipped with the best optical and mechanical components. An ideal microscope for daily cytology and anatomic pathology use. The 25 mm field
of view of the eyepieces and the plan, apochromatic objectives enable observations with perfect color rendering at high resolving powers. Specific attention to production methods resulted also in an excellent price/performance ratio. Please read this manual carefully before using this product to ensure correct and safe usage

• The contents of this manual are subject to change without notice
• The appearance of the actual product can differ from the models described in this manual
• Not all equipment mentioned in this manual has to be part of the set you have purchased
• All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

General safety instructions

Intended use: a non-medical device
This microscope is intended for general observation of cells and tissues, with transmitted/reflected illumination and with the specimen fixed on a slide
Intended use: medical device class l
This microscope is intended for observation and diagnostics of cells and tissues at hospitals or by physicians and veterinaries in private practice in pathology, anatomy and cytology applications. To be used with transmitted/ reflected illumination and with the specimen fixed on a slide. Physicians and veterinaries use microscopes to identify the different types of cells and spot abnormal cells. This product helps in identifying and treating diseases

Dangers associated with the operation

• Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator informs every user of existing hazards
• Danger of electrocution. Disconnect the power to the entire lighting system before installing, adding or changing any component
• Not to be used in corrosive or explosive environments
• Avoid direct exposure of eyes to the collimated light beam or direct light from the light guides or fibers
• To avoid a hazard to children, account for all parts and keep all packing materials in a safe place

Photobiological safety LED, important safety instructions

• Avoid direct eye exposure to any LED light source while switched on
• Before looking through the eyepieces of the microscope, lower the intensity of the LED illumination
• Avoid long and high-intensity exposure to LED light because this may cause acute damage to the retina of the eye

Prevention of biological and infectious hazards
Infectious, bacterial or viral biohazard substances under observation may be a risk to the health of humans and other living organisms. Special precautions should be taken during in vitro medical procedures :

• Biological hazards: keep a logbook of all the biological substances or pathogenic microorganisms that were under observation with the microscope and show it to everybody before they use the microscope or before they do some maintenance work on the microscope! Agents can be bacterial, spores, enveloped or non­ enveloped virus parties, fungi, or protozoa
• Contamination hazard:
• A sample that is properly endorsed with a cover glass never comes in direct contact with the microscope parts. In that case prevention of contamination lies in the handling of the slides; as long as the slides are decontaminated before use and are undamaged and treated normally, there is virtually zero risk of contamination
• A sample that is mounted on a slide without cover glass, can come in contact with components of the microscope and may be a hazard to humans and/or the environment. Therefore, check the microscope and accessories for possible contaminations. Clean the microscope surfaces and their components as thoroughly as possible. Should you identify possible contamination, inform the local responsible person in your organization
• Microscope operators could be contaminated by other activities and cross-contaminate components of the microscope. Therefore, check the microscope and accessories for possible contaminations. Clean the microscope surfaces and their components as thoroughly as possible. Should you identify possible contamination, inform the local responsible person in your organization. it is recommended to wear sterile gloves when preparing the slides and handling the microscope in order to reduce contamination by the operator
• Infection hazard: direct contact with the focusing knobs, stage adjustments, stage, and eyepieces/tubes of the microscope can be a potential source of bacterial and/or viral infections. The risk can be limited by using personal eyeshades or eyepieces. You can also use personal protections such as operation gloves and/or safety goggles, which should be changed frequently to minimize the risk
• Disinfectant hazards: before cleaning or disinfecting, check if the room is adequately ventilated. If not, wear respiratory protective gear. Exposure to chemicals and aerosols can harm the human eyes, skin, and respiratory system. Do not inhale vapors. During disinfection, do not eat, drink or smoke. Used disinfectants must be disposed of according to local or national regulations for health and safety

Disinfection and decontamination:

• Exterior casing and mechanical surfaces must be wiped with a dean cloth, dampened with a disinfectant
• Soft plastic parts and rubber surfaces can be deaned by gently wiping a clean doth, and dampened with a disinfectant. Discoloration can occur if alcohol is used
• The front lens of eyepieces and objectives are sensitive to chemicals. We recommend not to use aggressive disinfectants but to use lens paper or soft fiber-free tissue, damped in a deaning solution. Cotton swabs may also be used. We recommend you use personal eyepieces without eyeshades in order to minimize risk
• Never immerse or dip the eyepiece or objective into a disinfectant liquid! This will damage the component
• Never use abrasive compounds or dealers that may damage and scratch optical coatings
• Properly clean and disinfect all possible contaminated surfaces of the microscope or contaminated accessories before storing for future use. Disinfection procedures must be effective and appropriate
• Leave the disinfectant on the surface for the required exposure time, as specified by the manufacturer. If the disinfectant evaporates before the full exposure time, reapply the disinfectant on the surface
• For disinfection against bacteria, use a 70% .aqueous solution of isopropanol (isopropyl alcohol) and apply for at least 30 seconds. Against viruses, we recommend referring to specific alcohol or non-alcohol based disinfection products for laboratories

Before returning a microscope for repair or maintenance through a Euromex dealer, an RMA (return authorization form) together with a decontamination statement must be filled in! This document – available from Euromex for any reseller- must be shipped together with the microscope at all times

Reference documents:
World Health Organisation:
https://www.who.int/ihr/publications/biosafety-video-series/en
Robert Koch Institut:
https://link.springer.com/content/pdf/10.1007/500103-013-1863-6.pdf
US Centre fo r Disease Control and prevention
https://www.cdc.gov/infectioncontrol/guidelines/disinfection/index.html

Handle with care

• This product is a high-quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can affect the precision of the instrument

Handling the LED
Note; Always disconnect the power cord from your microscope before handling the LED bulb and power unit and allow the system to cool down for approximately 35 minutes to avoid burns

• Never touch the LED with your bare hands
• Dirt or fingerprints will reduce the life span and can result in uneven illumination, lowering the optical performance
• Use only original Euromex replacement LEDs The use of other products may cause malfunctions and will void the warranty
• During the use of the microscope the power unit will get hot; never touch it while in operation and allow the system to cool down approximately 35 minutes to avoid burns

Dirt on the lenses

• Dirt on or inside the optical components, such as eyepieces, lenses, etc., affects the image quality of your system negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving fingerprints on the lenses and dean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please, read the deaning instructions further on in this manual

A model with rechargeable batteries

• Always disconnect the power cord from the microscope before you replace the rechargeable batteries
• The rechargeable batteries must not be thrown away as regular trash but should be taken to special waste collection sites, according to your local or national regulations
• Risk of explosion: when removing the rechargeable batteries, do not throw the batteries into fire or any other heat source
• Do not replace the rechargeable batteries with non-rechargeable batteries
• Avoid extreme environmental conditions and temperatures which could affect the rechargeable batteries and lead to fire, explosion or leakage of hazardous substances
• If the rechargeable batteries have leaked, avoid contact of the chemicals with skin, eyes, and mucous membranes
• When in contact with the chemicals, flush the affected areas immediately with plenty of fresh water and seek medical attention

Environment, storage, and use

• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration-free, and level surface in order to prevent this instrument to fall thereby harming the operator
• Do not place the product in direct sunlight
• The ambient temperature should be between S to +40C and humidity should be between 80% and 50%
• Although the system is anti-mold treated, installing this product in a hot, humid location may still result in the formation of mold or condensation on lenses, impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse focus knob past its farthest point as this will damage this product
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat (fre hazard)
• Keep the microscope away from walls and obstructions for at least approximately 15 cm
• Never turn the microscope on when the dust cover is in place or when items are placed on the microscope
• Keep flammable fluids, fabric, etc. well out of the way

Disconnect power
Always disconnect your microscope from power before doing any maintenance, cleaning, assembling or replacing LEDs to prevent electric shocks
Prevent contact with water and other fluids
Never allow water or other fluids to come in contact with your microscope, this can cause short-circuiting your device, causing malfunction and damage to your system

Moving and assembling

• This microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage, or head
• When needed, move the microscope to two persons instead of one

Configuration, construction and controls
This chapter describes the main parts and functions of the Delphi-X Observer

movement
2H| Filter selector| 2s| Coarse and fine focusing control knobs
2l| Light selector| 21| Care on/off button
2J| Light intensity control knob| 2U| iCare sensor
2K| Collector lens|

Assembling Delphi-X Observer
This chapter describes the steps that need to be taken to assemble the Delphi-X Observer microscope. Euromex Microscopes will always try to keep the number of assembly steps for their customers as low as possible but there are some steps that need to be taken. The steps mentioned on the following pages are not always necessary but described for your convenience nonetheless:

The diagram shows the order of each component’s installation

Attaching the LED lamp chamber
| | Step 9| Attaching the phototube

Step 1/Attaching the focus cassette

• Attach the focus cassette according to the path shown in figure 1
• The dovetail slot needs to be aligned with the slot of focusing cassette
• Slide it down until it reaches the locking pin
• Then use the hex wrench tool to tighten the screw shown as (in figure 2)

Step 2]Attaching the mechanical X/Y stage

• Tum the coarse focus knob until the elevating section is brought to the lowest position
• Attach the mechanical object stage according to fgure 3 by aligning the stage above the ring of the focus cassette
• Fix the mechanical stage into place with a screw (figure 4)

3 Step ]Attaching Nosepiece (figure 5)

• Slide the nose piece into the slot
• Fix into place with screw (II)

Step 4[Placing the microscope head (figure 6)

• Place the head by loosening the screw (Ill)
• Mount the head in its position inside the microscope arm
• Secure it by tightening the screw again

Step S[Placing the C-mount or photo port, onto the microscope head (figure7)

• loosen the screw (IV)
• Place either the C-mount or photo port and tighten the screw

Step 6[Placing and mounting the eyepieces (figure 8)

• First, remove the dustcover of the eyepiece tubes
• Insert the eyepieces into the eyepiece tubes

Step 7[Placing the condenser (figure 9)

• Use the condenser height control knob (V) to lower the condenser holder to the lowest position
• Insert the condenser into the holder as shown unfigured
• Then secure the condenser by fixing the screw indicated
• Centering the condenser is described later in this manual

Step 8]Attaching the LED Lamp HOUSING UNIT (figure 10A)

• Slide the lamp unit (Halogen or LED) into position at the back of microscope base
• Use the wrench screw tool to secure the bolt (VI)

Step ll I Connecting the power cord
The Delphi-X Observer microscopes support a wide range of operating voltages: 100 to 240 V. Please use a grounded power connection

• Make sure the power switch is off before connecting.
• Insert the connector of the power cord into the Delphi-X Observer power socket (figure 108), and make sure it connects well
• Insert the other connector into the mains socket, and make sure it connects well
• Put the power switch to ON

Do not bend or twist the power cord, it will get damaged. Use the special cord supplied by Euromex. If it is lost or damaged, choose one with the same specifications

Operation

Placing the slide
(figure 11)

• Lower the condenser slightly from the uppermost position by turning the condenser focus knob
• Open both aperture and field diaphragm entirely
• Bring the 4x objective (or lowest objective in your configuration) into the optical path by rotating the nosepiece until the right objective dicks into position
• Pull back the spring damp of the specimen holder and gently place the slide into position
• Gently release pressure from the spring clamp so it softly moves back in position securing the slide
• Use the X and Y axis control knobs of the mechanical stage to move to area of interest of the slide into the light path

Adjusting the tension of X and Y axis control knobs
(figure 12

• The degree of tension on the X and Y-axis control knobs can be adjusted
• To do that, drawdown handwheel (A) and find two adjusting rings (B, C)
• By rotating these rings the movement of the knobs can be set lighter and heavier
• Ring B is used for adjusting the X direction
• Ring Cis is used for adjusting the Y direction

Switching between light sources
(figure 13)

Next to the intensity controller, there is a button for switching between transmitted and reflected illumination. The standard brightfield configuration used for this manual does not have this option

• When the button is pushed in, the light is set to reflected mode
• When the button is pushed out, the light is set to transmitted mode (standard)
  

Getting the specimen in focus
(figure 14)

• Use the coarse control knobs to adjust the focus quickly and roughly
• Get the specimen into sight through the eyepieces
• Then use the fine focus control knob to adjust the focus in detail

Adjusting the coarse focus tension
(figure 15)
Next to the right side coarse focus know there is a ring for adjusting the coarse focus tensions This can be used to make the coarse control move lighter or heavier, according to user preference

Setting the focus lock
(figure 16
Next to the left side coarse focus know there is a ring setting the focus lock. The focus lock can be used to limit the maximum position of the stage at a certain height. This is ideal for preventing objectives to get damaged, slides from breaking, or setting the stage at a reference height

• Move the stage to the desired height then fx the ring tight to lock the mechanical stage’s maximum height
• The stage still can be lowered but the highest position is now limited to the set position
• Release the ring to undo the focus lock

Switching the fine focus knobs
(figure 17 and 18
The fine focus knobs can be switched from left to right to meet user preference

• Pull the knobs with moderate force to release the magnet which is holding the knobs onto the stand
• Attach the magnets onto the holder and let it grab the knobs again to mount them onto the holder

Adjust interpupillary distance
The Delphi-X Observer has an interpupillary distance range of 47 to 78 mm. The correct interpupillary distance is reached when one round image is seen in the field of view
This distance can be set by either pulling the tubes towards each other or pulling them from each other. This distance is different for each observer and this should be set individually. When more users are working with the microscope it is recommended to remember your interpupillary distance for a quick setup during new microscopy sessions

Adjust the diopter of the eyepieces
(figure 19
In order to compensate for human eye differences, distortion, and thickness differences in cover glasses and tune for the best parfocality between objectives, one can use the diopter to do so. Take a well-prepared slide for your reference:

• Set (both) the diopter adjustments of the eyepieces to 0

• Select the 10x objective, look for an interesting area on the specimen and focus on this area

• Select the 40x objective and focus on the specimen
  Warning: don’t change the coarse and fine adjustment anymore

• With your dominant eye open (lose your other eye), rotate the diopter adjustment from +” to” until the selected area get as sharp as possible

• If during this operation the image becomes unsharp, take your eyes from the eyepieces and turn the diopter adjustment, without looking into the eyepieces, a few divisions back from-” to +

• Look into the eyepiece again and turn the diopter adjustment from ‘+’to ‘ until the selected area on your specimen gets the optimal sharpness

• Repeat for your non-dominant eye, and with the second diopter

Verification:

• Take your eyes from the eyepieces and look for 2 seconds to a far point in the room in order to reset ” your eyes
• Look again into the eyepieces.If the adjustment is not good, repeat the operation till you reach the same sharpness for the 10x and 40x objective without touching the  coarse and micrometric adjustments

The correct eyepoint

(figure 20)
The eyepoint is the distance from the eyepiece to the user’s pupil. To obtain the correct eyepoint, move the eyes towards the eyepieces until a sharp image is reached at a full field of view

Select eyepiece and camera light throughput
(figure 21
The Delphi-X Observer gives users the option to select out of three output types, giving large flexibility when using cameras. The push/pull rod on the side of the microscope head can be set in 3 positions:
POSITION 1]The optical light path is sent to the eyepieces only. Ideal for when no camera is used
POSTI0N 2]The optical light path is sent to the eyepieces for 209% only. The ideal standard setting for when a camera is used
POSTI0N 3]The optical light path is sent to the camera only. Ideal for when the camera is used at low light imaging

These positions are indicated on the head as well for user convenience

Centering the condenser
(figure 22)

• Move the condenser to the top position (1)
• Focus on a specimen using the smallest objective (f.e. 4x or 10x objective)
• Close the field diaphragm (2)
• Use screws (figure 23) to move the field diaphragm to view center
• Open the field diaphragm carefully to the outside of the field of view to ensure the field diaphragm is in the center and so the condenser has been centered properly

The aperture diaphragm (figure 24/3) should be used to adjust the numerical aperture, not to adjust image brightness. When the aperture diaphragm is opened to the 70 80% of the objective aperture the ideal position is reached The simplest way to do this is to use the markings on the condenser
For example: when a 40x objective with N.A.0.65 is used, one can set the condenser to 70 80% 0f0.65 which is 0.45to 0.58

Using LED WITH frosted filter
(figure 25)
For LED models there is only 1 push button

• Push the button in for placing the frosted filter into the light path

LED version with frosted filter

Using HALOGEN WITH LBD, ND 6, and ND25 filters
(figure 26)
The halogen version has three filter options:

1. L8Dis a filter for increasing color temperature
2. ND2Sis a filter with 25% light transmittance
3. ND6 is a filter with 69% light transmittance

Halogen version with LBD and two ND filt ers

iCare sensor
(figure 27)
The unique iCare Sensor is developed to avoid unnecessary loss of energy. The illumination of the microscope automatically switches off shortly after the user steps away from his or her position

• Pushing the iCare button will re-active the light
• The iCare function is turned on by default
• To turn off the iCare function push the iCare button for4 a seconds
• The function will be deactivated and the bright LED will dim to indicate the the function has to be turned off
• Repeating this step will turn the function back on

Replacing the fuse
(figure 28)
The fuse is placed in a drawer

• To open it push the drawer aside with a screwdriver
• Take out the drawer and replace the fuse gently

Cleaning optics

Howtokeepthe optics clean?
Dust and dirt particles have a negative effect on image quality. Keeping the optical system of your microscope lean is essential for the best image quality and overall lifetime of your microscope. Dust and dirt on optical elements such as lenses, prisms, and filters that are left unattended can become difficult -or even impossible to remove and may cause mold

FIGURE A

• Place your objective or eyepiece on a secure location
• Objectives can be screwed into the cover of an objective case
• Eyepieces can be placed in the microscope box
• Condensers and collector lenses can remain in place in the microscope

FIGURE B

• To prevent scratches on coatings and optical glass try to remove dirt and dust that sticks to the optical surface first with an air-blower or with pressurized dry air (oil-free and under moderate pressure version only)

FIGURE C

• Use an absorbent lens paper or cotton swab.
• Dampen a swap or towel with a small amount of lens cleaning fluid or cleaning mixture (either pure isopropanol or a mixture of 7 parts ether and 3 parts alcohol)

FIGURED

• (lean the lens by using the tip of the cotton swap or the lens paper. Use enough lens paper so that solvents do not dissolve oils from your hands which can make their way through the paper onto the coated surface
• When deaning a large lens surface, wipe with little pressure from the center towards the periphery in a circular motion. Do not use a zig-zag motion
• Discard each lens paper or cotton swap after a single-use

FIGURE E

• Wait until the cleaning fluid is evaporated, or speed up this process by using pressurized dry air
• Check if the surface is dean by using a magnifying glass
• Place the cleaned item back on the microscope

Please note that cleaning of the optical surfaces indicated in this instruction only applies to external surfaces of objectives, eyepieces, filters, and condensers. Internal surfaces must always be done by your Euromex microscopes distributor

Troubleshooting

Proper use and maintenance ensure the best performance of your Delphi-X Observer. If problems occur this chapter explains how to resolve most issues. Please make sure this chapter is read and checked before contacting your Euromex distributor for service. If a problem is not described in this list or the suggested solution does not bring the result needed, please contact your Euromex distributor

There is no light
from the lamp| No power| Check f the power cable is connected welt try other
power cable
The bulb is not inserted| Remove the Nib and place it back
The bulb is defective| Remove the bah and place it bock
The transmitted-reflected illumination switch k
in the wrong position| Change position el the switch
The fuse has blown| Replace the fuse
There is no power from the mains connection| Replace it
The lamp burns out
suddenly| Poor bulb quatty| Use S specified  lamp to replace t f the problem is
not solved, contact your a reseller
The bulb flickers a the brightness k  vertiginous| The bolo k a the end of its We span| Replace the ‘nib
The but* is not inserted entirely into the holder| Remove S bulb and replace it
Care sensor does not turn off the illumination| There ere other things in front of the microscope
(within 1 meter)| Clear dr object if a 1-meter radius
The Cate function k turned off| Press and had S iCare button 42r3 seconds b
activate the function

OPTICAL SYSTEM

The edge of the
field of view is dark
or the brightness is
not uniform| The nosepieces are not in the located position
(objective and light path are rot coaxial)| Locate the nosepiece properly where It clicks
The age of the lamp is not centered| Center the lamp
The lens (objective, condenser, eyepiece a collector) is dirty| Clean t thoroughly
Find dust and stain in the field of view| Fist rotate the eyepieces if the dust moves:| Clean the eyepieces
Next more the stage with side, if the dust moves:| Clean the side a replace the slide
Next move the condenser up and down,if the dust moves (using 4 a 10x objective):| Clean the top of the condenser
Next change objective, if the dirt is no longer visible:| Clean S bottom lens ci the objective
If the problem remains:| Cleat the collector In
Image quality is not optimal (resolution a contrast)| There is ro cove sap on the specimen| Add coverslip
The cover sip is too thick a to thin| Use the standard coverslip (0.17mm)
The specimen is placed upside down| Turn side around
There is of on a non-of lens, this often happens b the 40x objective| Clem the objective
There are stains m the lens (including
condenser, objective, eyepiece, and collector)| Clean the optical elements
No of is used fa the 100x of objective| Use Euromex immersion al (P65255)
There ere bubbles h the of| Try to remove S bubbles a. create a new slide
Wrong of is used| Use Eurornex immersion a (P83255)
The size cf the aperture S.aphragm is too large| Close the diaphragm
The size of the aperture diaphragm is too small| Open S diaphragm
| The position of the condenser is too low| Adjust the position
---|---|---
The periphery of the image is dark/ unclear (unevenly
illuminated)| For low magnification objectives (4x, 2x) the
the swing-out condenser was not used correctly| Swing out the top lens of the condenser
Diaphragm(s) closed too far| Open the diaphragms
The lamp unit is not placed correctly| Take out lamp unit and re-install
Incorrect position of the light path switching lever| Set to the right position
The nosepiece is not in the right position| Turn the nosepiece until it ‘clicks’ into position
One side of the
image is dark| The condenser is not centered correctly| Center the condenser
The condenser is placed inclined in it’s holder| Install the condenser again and center it
The nosepiece is not in the right position| Turn the condenser until it ‘clicks “into position
The diaphragm is not centered| Center diaphragm
One part of the
image is not in focus. Part of the image becomes out of focus while moving the specimen| The condenser is placed inclined in it’s holder| Install the condenser again and center it
The stage is tilted| Re-install the stage making sure it is leveled
The specimen slide is not placed flat on the stage| Replace the slide on the stage
The nosepiece is not in the right position| Turn the nosepiece until it ‘dicks” into position
The specimen slide is not prepared well| Try a specimen of known quality and confirm
Image can not be focused while the stage is in the highest position| The focus lock system Is secured at the wrong position| Release the focus lock, focus and lock again
The stage is not installed correctly| Re-install the stage making sure it is leveled
The image through
the eyepieces is
shown as a double
image or half
moons appear| Interpupillary distance has not been set correctly| Perform interpupillary adjustment
Dioptre adjustment has not been done correctly| Perform diopter adjustment
Eyes are getting
tired| Interpupillary distance has not been set correctly| Perform interpupillary adjustment
Dioptre adjustment has not been done correctly| Perform diopter adjustment
Brightness is not correct| Adjust the brightness using the intensity control knob or
filters
The image is too dark| Too low intensity set on intensity controller| Increase the intensity by rotating the controller
The size of the aperture diaphragm is too small| Adjust again
The position of the condenser is too low| Adjust the position
Poor bulb quality| Use the specified lamp
Diaphragm(s) closed too far| Open the diaphragms
Lightpath selector set in the wrong position| Select the 100:0 or 20:80 position
The bulb is at the end of it’s life span| Replace the bulb
The Kohler incident light is not in the center| Adjust the bolt of the Kohler incident light
The image is too bright| Too high intensity set on intensity controller| Decrease the intensity by rotating the controller
The size of the aperture diaphragm is too large| Adjust again
The position of the condenser is too high| Adjust the position
The image appears blue-ish, yellow-ish or orange-ish| Too low or too high intensity set on the intensity
controller (Halogen illumination only)| Increase or decrease the intensity by rotating the
controller, and use the ND filters
The bulb is at the end of its life span| Replace the bulb
The image cannot be focused when using high magnification objectives| The coverslip is too thick| Use the standard coverslip (0.17 mm)
The specimen is placed upside down| Turn slide around
The focus lock system is secured in the wrong position| Release the focus lock, focus and lock again
The objective touches the
specimen when the magnification is being changed| The cove slip is too thick| Eke the standard coverslip (017 min)
---|---|---
Focus lock system k seared at the wrong position| Release S focus lock, focus, and Jock again
Large focus deviation while changing objectives| Prime objective is placed incorrectly, not screwed in all the way| Make sure the right objective is used and screw t dl
tie way into the revolving nosepiece
The tension cf the X/Y controls d the stage are set to tight| Adjust tension to the proper setting
Diopter adjustment has not been done correctly| Perform copter adjustment
The slide does not
move, a moves too
heavily| The spearmen h not placed between the specimen holder| Place specimen between the holder
The tension cf the X/Y controls cf the stage are set to tight| Adjust tension to the proper setting

Euromex Microscopen bv+ Papenkamp 20+6836 BD Arnhem Th e Netherlands
T+31(0)26323221+[email protected] + wwweuromex.com

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