SKC UMEx 400 Passive Sampler for Aliphatic Amines Owner’s Manual

SKC UMEx 400 Passive Sampler for Aliphatic Amines Owner’s Manual

Operating Instructions

The SKC UMEx 400 Passive Sampler collects aliphatic amines in the range of 0.5 to 10 ppm. The UMEx 400 Passive Sampler contains a tape treated with 1 -Naphthyl-isothiocyanate (NITC). Each sampler incorporates a “blank/ correction” section in addition to the active sampling section. Analysis is by high-performance liquid chromatography with UV detection (HPLC-UV) for identification of aliphatic amines that may be present in the sample

Designed for single use, the SKC UMEx 400 Passive Sampler is packaged in an aluminized pouch for easy sample transport to a laboratory.

• UMEx Sampler with sliding cover in “off” position

• UMEx Sampler with sliding cover in “on” position

Performance Profile

Sampling Rates:

• Concentration Range: 0.5 to 10 ppm

• Detection Principle: Formation of a stable amine derivative, made from a reaction of the aliphatic amine with NITC

• Analysis Method: Analysis by HPLC with UV detection at 254 nm

• Accuracy: ± 25% for all aliphatic amines except methyl amine ± 35% for methyl amine

• Shelf-life: 12 months from date of manufacture in refrigerator

• Storage: Before use: ≤ 39.2 F (4 C)
  

• After use: Samples can store at ambient temperatures for 14 days For storage greater than 14 days, store at ≤ 39.2 F (4 C).
  Do not store with food.

• Temperature Effects: No effect on sampling rate between 50 and 86 F (10 and 30 C)

• Humidity Effects: No effect on sampling rate from 10 to 80% relative humidity (RH)

• Wind Velocity Effects: No effect from 5 to 100 cm/sec

• Interferences: None found; highly specifi c for amines

• Validation: Partial

• Dimensions: 3.4 x 1.1 x 0.35 in (8.6 x 2.8 x .89 cm)

• Weight: 0.38 oz (10.8 g)

• Slide Cover: Blue

Sampling Instructions

Cautions:

• Store at ≤ 39.2 F (4 C) before use.
• Do not store with food.
• Before sampling, check the expiration date on the label on the outside of the pouch. Do not use after the last day of the month indicated.
• SKC recommends using gloves when handling chemically treated media.
• UMEx samplers are designed for single use. Do NOT reuse UMEx samplers.

1. Open the pouch and remove the sampler. Do not discard the pouch; use it to send sampler to the laboratory. Store pouch away from potential amine sources.
2. Enter appropriate information on the label.
3. Position the sampler on a worker’s collar for personal sampling or in an appropriate location for area sampling.
4. Slide the sampler cover to the “on” position to begin sampling. Enter the sample start time in the space provided on the back of the sampler.
5. After sampling for the desired time, up to 8 hours, slide the sampler cover to the “off ” position to stop sampling. Enter the sample stop time in the space provided on the back of the sampler.
6. Place the sampler in the original pouch.
7. Seal the pouch. Send to an accredited laboratory for analysis.

Analysis Instructions

Calibration Standards

To prepare standards of the amine derivatives, simply add a known amount of the amine to a vial containing 3 ml of acetonitrile and a section of the NITCtreated tape.

Sample Preparation

1. Remove the sampler from the pouch and the sliding cover from the sampler. Use forceps (cleaned with acetonitrile) to lift out the reactive tape from each section. Place each in a sealed vial. This provides a sample and a blank.
   Note: The blank/correction section has an indentation for easy identifi cation.

2. Add 3.0 ml of acetonitrile to each vial.

3. Perform sample analysis on both tapes.
   

Sample Analysis

1. The amine derivative is desorbed from both the sample and control tapes by placing each in its own glass vial containing 3.0 ml of acetonitrile and shaking them for one minute by hand.
2. A 20-microliter portion of this sample is injected into a liquid chromatograph and analyzed using a C18 column and a mobile phase consisting of 60% acetonitrile and 40% water.
3. The amine derivative is detected by UV detection at 254 nanometers (nm).
4. Quantitation is performed using calibration standards prepared as described in Calibration Standards above.

References

Lindahl, R., Levin, J.O., and Andersson, K., “Determination of Volatile Amines in Air by Diff usive Sampling, Thiourea Formation, and High-performance Liquid Chromatography,” Journal of Chromatography, 643, 1993, pp. 35-41

• Limited shelf-life. Do not store with food.
  † Storage at ≤ 39.2 F (4 C) required
  ‡ UMEx samplers are designed for single use only. Do NOT reuse UMEx samplers.

SKC Limited Warranty and Return Policy

SKC products are subject to the SKC Limited Warranty and Return Policy, which provides SKC’s sole liability and the buyer’s exclusive remedy. To view the complete SKC Limited Warranty and Return Policy, go to skcinc.com/warranty.

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>