LOLIGO SYSTEMS OmniCTRL pH CO Software User Guide

QUICK GUIDE | OmniCTRL (pH/CO₂)

FIRST TIME USE

1. Download the latest version of OmniCTRL from our website: loligosystems.com/downloads Follow the installation instructions on the screen and then restart the PC.
2. Power on each WTW meter by pressing the Power button. Press and hold the ENTER button to enter the Storage & config menu (arrow, 3c). Select System > Interface > Baud rate, and set the baud rate to 19200. Press the F1 button until you return to the start screen.

SETUP

3. Insert the Loligo® license dongle (3a) in a USB port on the PC to unlock the full software.
   OmniCTRL will run in demo mode, if the license dongle is not inserted. Running OmniCTRL in demo mode will enable virtual hardware that simulates data during an experiment (i.e., you cannot use real hardware).

4. Connect the Long range Bluetooth dongle to a USB port on your PC, and let it initialize (3b).
   Disable any other Bluetooth radios on your PC. Connect the PowerX4 power strip to a grounded wall outlet (4a).

5. Connect the WTW meter to a USB port on your PC. Power on the meter. If you are using a Witrox oxygen instrument… Connect the Witrox (4b/c) to a power source (PC USB or USB adapter). Click the red Power button. The blue connect icon will now flash blue (for 5 min.) indicating that the Witrox is in pairing mode. Open OmniCTRL > Devices > Choose Scan for new devices. When scanning is complete, press and hold the F2 button on the WTW meter until the Autom. USB Output menu appears. Change Interval rate, if necessary. Choose Continue to exit. The data shown on the WTW meter display will now show on the WTW illustration display in OmniCTRL (arrow, 5).
   Now choose the type of regulation you want each instrument to perform by clicking the regulation buttons on the left. The buttons will turn green when active:
   = No regulation
   = Decrease parameter level
   = Increase parameter level
   = Increase/Decrease parameter level
   The type of regulation will be assigned to a relay on the available PowerX4(s) (arrow, 5). Make sure to connect the required hardware (i.e. solenoid valve) to the correct relay on the PowerX4.

6. Each WTW probe and Witrox oxygen sensor must be calibrated before first time use. See step 12-14 for more information.

RUNNING AN EXPERIMENT

7. Repeat step 3-4, and open OmniCTRL. Go to Devices > Choose Use current configuration, and complete step 5 from “When scanning is complete…”

8. It may be necessary to perform a new WTW probe or Witrox oxygen sensor calibration (see step 12). If regulating CO₂, a pH -> CO₂ conversion is needed (see step 11).

9. Main menu > Experiment: See real-time data and start regulating water quality. Start regulation: Click on for each channel to change setpoint, regulation type etc. (arrow,
   Choose regulation type (Off, Manual, Automated or File), and change regulation settings, if necessary. View the protocol by clicking the button. Click Apply to start regulating. Start logging: Click Start logging (arrow, 9) > Choose file destination > Logging starts

10. Main menu > Protocol designer: Create custom protocols for special regulation requirements (e.g. simulating diurnal curves). In the settings panel to the right, click on a given graph illustration to determine the regulation pattern (arrow, 10). Additionally, change the regulation 10 settings underneath to customized the protocol to your liking. The protocol will be displayed as a graph on the left. Click Export to save the current protocol as an editable text file (arrow, 10). Load the saved protocol file during an experiment (i.e. choose File as regulation type).

CALIBRATION, CLEANING, AND MAINTENANCE

11. pH -> CO₂ conversion: Calibrate (13) the WTW SenTix HWD pH probe before conversion.
    Prepare three water samples of the same quality (i.e. buffer capacity) as the water used for experiments, and keep water temperature constant and the same.
    Use a gas mixing pump (Wösthof), mass flow cells, or ready-made gas mix to bubble the three samples at three different pCO₂ levels within the range of your experiment min/max pCO₂ level. Place the SenTix HWD pH probe in the first water sample, click the to measure pH, and then type in the corresponding pCO₂ level. Click + to add the next measurement point (11). Perform three measurements per water sample to ensure stabilized readings. The conversion is now done.

12. When to calibrate… In general, you should perform a new sensor calibration if you experience sensor performance issues (e.g. signal drift). Otherwise, a new calibration is recommended:
    • WTW Oxi 3310 (CellOx 325): When the probe symbol flashes (after the set calibration interval has expired (150 days as standard)) or when using a new probe.
    • WTW Cond 3310 (TetraCon 325): Every 6 months or when using a new probe.
    • WTW pH 3310 (SenTix HWD): When the probe symbol flashes (after the set calibration interval has expired (7 days as standard)) or when using a new probe.
    • Witrox: When the % air sat. level drifts with > +/-2 % or when using a new oxygen sensor. The temp. probe is pre-calibrated and cannot be manually calibrated. Calibrate a WTW probe: Please see the respective WTW meter user manual for more instructions. Each manual can be found at: loligosystems.com/manuals

13. Calibrate a Witrox oxygen sensor: In OmniCTRL > Main menu > Calibration > Choose Witrox instrument. Click on the relevant channel ( CH1-CH4) to open the channel calibration menu

14. Select the type of temperature input (Witrox controlled or User defined) and then perform a Manual 2-point calibration (14 and 14.1):
    a. Place the sensor tip in a mixed air-equilibrated water sample. This can be achieved by purging atmospheric air into sample water, e.g. with an air pump.
    b. Wait for the phase reading (sensor signal) to stabilize and then click Read current values to save the current value as the HIGH calibration value (100 % air saturation).
    c. Transfer the sensor to an oxygen free water sample, e.g. by purging nitrogen gas into sample water or by dissolving ~10 grams of Na2 SO3 in 500 ml of distilled water.
    d. Wait for the phase reading to stabilize and then click Read current values to save the current sensor signals as the LOW calibration value (0 % air saturation).

15. How to clean… In general, clean each instrument and sensor between uses.
    • WTW probes: Please follow the cleaning instructions provided in the manual for the CellOx 325, TetraCon 325, or SenTix HWD probe. Each manual can be found at: loligosystems.com/manual 
    • Witrox oxygen sensor: Use a mild soap solution or bleach, and rinse with demi water.
    Then dry (15).
    • WTW and Witrox meters: Wipe the outside of the measuring instrument with a damp, lint-free cloth. Disinfect the housing with isopropanol as required. Avoid contact with acetone or similar detergents that contain solvents.
    Store all sensors in a dark, dry place between trials. Avoid exposing the fluorescent dye on the Witrox oxygen sensor to UV light. UV light will bleach the sensor dye and decrease the signal strength (amplitude).

SETUP GUIDE

References

• loligosystems.com/manuals

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