Loligo Systems Witrox pH 3310 1 Oxygen Meter User Guide

: June 17, 2024
: Loligo Systems

Table of Contents

FIRST TIME USE
SETUP
RUNNING AN EXPERIMENT
CALIBRATION, CLEANING, AND MAINTENANCE
Omni CTRL system
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

Loligo Systems Witrox pH 3310 1 Oxygen Meter

Loligo Systems Witrox pH 3310 1 Oxygen Meter

FIRST TIME USE

Download the latest version of Omni CTRL from our website: loligosystems.com/downloads Follow the installation instructions on the screen and then restart the PC
Power on each WTW meter by pressing the Power button. Press and hold the ENTER button to enter the Storage & config menu (arrow, 3c). Select System > Interface > Baud rate, and set the baud rate to 19200. Press the F1 button until you return to the start screen.

SETUP

Insert the Loligo® license dongle (3a) in a USB port on the PC to unlock the full software.
Omni CTRL will run in demo mode, if the license dongle is not inserted. Running Omni CTRL in demo mode will enable virtual hardware that simulates data during an experiment (i.e., you cannot use real hardware).
Connect the Long range Bluetooth dongle to a USB port on your PC, and let it initialize (3b). Disable any other Bluetooth radios on your PC.
Connect the PowerX4 power strip to a grounded wall outlet (4a).
Connect the WTW meter to a USB port on your PC. Power on the meter.
If you are using a Witrox oxygen instrument… Connect the Witrox (4b/c) to a power source (PC USB or USB adapter). Click the red Power button. The blue connect icon will now flash blue (for 5 min.) indicating that the Witrox is in pairing mode.
Open Omni CTRL > Devices > Choose Scan for new devices. When scanning is complete, press and hold the F2 button on the WTW meter until the Atom. USB Output menu appears.
Change Interval rate, if necessary. Choose Continue to exit. The data shown on the WTW meter display will now show on the WTW illustration display in Omni CTRL (arrow, 5).

Now choose the type of regulation you want each instrument to perform by clicking the regulation buttons on the left. The buttons will turn green when active:
= No regulation
= Increase parameter level
= Decrease parameter level
= Increase/Decrease parameter level
The type of regulation will be assigned to a relay on the available PowerX4(s) (arrow, 5). Make sure to connect the required hardware (i.e. solenoid valve) to the correct relay on the PowerX4.
Each WTW probe and Witrox oxygen sensor must be calibrated before first time use. See step 6 12-14 for more information.

RUNNING AN EXPERIMENT

Repeat step 3-4, and open Omni CTRL. Go to Devices > Choose Use current configuration , 7 and complete step 5 from “When scanning is complete… ”
It may be necessary to perform a new WTW probe or Witrox oxygen sensor calibration (see step 12). If regulating CO₂, a pH -> CO₂ conversion is needed (see step 11).
Main menu > Experiment: See real-time data and start regulating water quality.
Start regulation: Click on for each channel to change setpoint, regulation type etc. (arrow, 9). Choose regulation type (Off, Manual, Automated or File), and change regulation settings, if necessary. View the protocol by clicking the button. Click Apply to start regulating.
Start logging: Click Start logging (arrow, 9) > Choose file destination > Logging starts
Main menu > Protocol designer: Create custom protocols for special regulation requirements (e.g. simulating diurnal curves). In the settings panel to the right, click on a given graph illustration to determine the regulation pattern (arrow, 10). Additionally, change the regulation settings underneath to customized the protocol to your liking. The protocol will be displayed as a graph on the left. Click Export to save the current protocol as an editable text file (arrow, 10).
Load the saved protocol file during an experiment (i.e. choose File as regulation type).

CALIBRATION, CLEANING, AND MAINTENANCE

pH - > CO₂ conversion: Calibrate (13) the WTW SenTix HWD pH probe before conversion.
Prepare three water samples of the same quality (i.e. buffer capacity) as the water used for experiments, and keep water temperature constant and the same.
Use a gas mixing pump (Wösthof), mass flow cells, or ready-made gas mix to bubble the three samples at three different pCO₂ levels within the range of your experiment min/max pCO₂ level.
Place the SenTix HWD pH probe in the first water sample, click the to measure pH, and then type in the corresponding pCO₂ level. Click to add the next measurement point (11). Perform three measurements per water sample to ensure stabilized readings. The conversion is now done.
When to calibrate… In general, you should perform a new sensor calibration if you experience sensor performance issues (e.g. signal drift). Otherwise, a new calibration is recommended:

  *  **WTW Oxi 3310 (CellOx 325)** : When the probe symbol flashes (after the set calibration interval has expired (150 days as standard)) or when using a new probe.
  *  **WTW Cond 3310 (TetraCon 325)** : Every 6 months or when using a new probe.
  *  **WTW pH 3310 (SenTix HWD)** : When the probe symbol flashes (after the set calibration interval has expired (7 days as standard)) or when using a new probe.
  *  **Witrox:** When the % air sat. level drifts with > +/-2 % or when using a new oxygen sensor. The temp. probe is pre-calibrated and cannot be manually calibrated.

Calibrate a WTW probe : Please see the respective WTW meter user manual for more 13 instructions. Each manual can be found at: loligosystems.com/manuals
Calibrate a Witrox oxygen sensor : In Omni CTRL > Main menu > Calibration > Choose Witrox instrument. Click on the relevant channel ( CH1-CH4) to open the channel calibration menu (14). Select the type of temperature input (Witrox controlled or User defined) and then perform a Manual 2-point calibration (14 and 14.1):
How to clean… In general, clean each instrument and sensor between uses.

  *  **WTW probes** : Please follow the cleaning instructions provided in the manual for the CellOx 325, TetraCon 325, or SenTix HWD probe. Each manual can be found at: loligosystems.com/manual
  *  **Witrox oxygen sensor** : Use a mild soap solution or bleach, and rinse with demi water. Then dry (15).
  *  **WTW and Witrox meters** : Wipe the outside of the measuring instrument with a damp, lint-free cloth. Disinfect the housing with isopropanol as required. Avoid contact with acetone or similar detergents that contain solvents.

Calibration, Cleaning, And Maintenance

Store all sensors in a dark, dry place between trials. Avoid exposing the fluorescent dye on the Witrox oxygen sensor to UV light. UV light will bleach the sensor dye and decrease the signal strength (amplitude).