Leica M125 B Stereo Microscope User Manual
- June 16, 2024
- Leica
Table of Contents
M125 B Stereo Microscope
Product Information
Specifications
-
Model: Leica M125 B
-
Model: Leica M165 C B
-
Designed for routine examinations of cell and tissue cultures,
liquids, and sediments -
Complies with Council Directive 98/79/EC concerning in vitro
diagnostics -
Can be used in clean rooms
Product Usage Instructions
Function of the Microscopes
The Leica M125 B and Leica M165 C B microscopes are designed for
routine examinations of cell and tissue cultures, liquids, and
sediments. This includes examining specimens taken from the human
body for the purpose of gaining information about physiological or
pathological conditions or inborn anomalies, or testing for safety
and compatibility for potential recipients, or for monitoring
therapeutic measures.
It is important to use the microscopes only for their intended
purposes and within the specifications provided by Leica
Microsystems CMS GmbH. Any other use may render the Declaration of
Conformity invalid.
The microscopes are suitable for use by trained laboratory
personnel and comply with EN 60601-1 for electrical safety when
connected to medical instruments in accordance with 60601-1.
However, they are not suitable for examining potentially infectious
specimens.
General Notes
Use in clean rooms: The Leica M series can be
used in clean rooms without any problems.
Cleaning: When cleaning the microscopes, do not
use any unsuitable cleaning agents, chemicals, or techniques. Avoid
using chemicals on colored surfaces or accessories with rubberized
parts to prevent damage or contamination. Refer to the brochure
“Cleaning of Microscope Optics” for specific instructions on
cleaning glass surfaces and objectives. The brochure can be
downloaded from http://www.leica-microsystems.com/products/.
Servicing: Repairs should only be carried out
by Leica Microsystems-trained service technicians using original
Leica Microsystems spare parts.
Responsibilities of person in charge of
instrument: Ensure that the Leica stereomicroscope is
operated, maintained, and repaired by authorized and trained
personnel only. Special solutions and accessories for use in clean
rooms may be available upon request.
FAQ
Can the microscopes be used for examining infectious
specimens?
No, the microscopes are not suitable for examining potentially
infectious specimens.
Where can I find instructions for cleaning the glass surfaces
and objectives?
You can download the brochure “Cleaning of Microscope Optics”
from http://www.leica-microsystems.com/products/.
Select your product and go to the Download page.
Who should carry out repairs on the microscopes?
Repairs should only be carried out by Leica Microsystems-trained
service technicians using original Leica Microsystems spare
parts.
Living up to Life
User Manual
Leica M125 B Leica M165 C B
Copyright
All rights to this documentation are held by Leica Microsystems CMS GmbH.
Reproduction of text or illustrations (in whole or in part) by print,
photocopy, microfilm or other method (including electronic systems) is not
allowed without express written permission from Leica Microsystems CMS GmbH.
The instructions contained in the following documentation reflect state-of-
the-art technology. We have compiled the texts and illustrations as accurately
as possible. Still, we are always grateful for comments and suggestions
regarding potential mistakes within this documentation.
The information included in this manual may be changed without prior notice.
Revision 1.0, published March 14, 2013 by:
Leica Microsystems Wetzlar GmbH Ernst Leitz-Straße 17-37 D-35578 Wetzlar
(Germany) http://www.leica-microsystems.com
Responsible for contents: Marketing CMS
Leica M series
User Manual
2
Function of the Microscopes
The Leica M125 B and Leica M165 C B microscopes, for which this User Manual has been written, are designed for routine examinations of cell and tissue cultures, liquids, and sediments. This includes examining specimens taken from the human body for the purpose of gaining information about physiological or pathological conditions or inborn anomalies, or testing for safety and compatibility for potential recipients, or for monitoring therapeutic measures.
other purposes than those for which they are intended or not using them within
the specifications of Leica Microsystems CMS GmbH. In such cases, the
Declaration of Conformity shall be invalid.
· These (IVD) instruments are not intended for use in the patient environment
defined by DIN VDE 0100-710. Nor are they designed to be combined with medical
instruments in accordance with EN 60601-1. If a microscope is electrically connected to a medical instrument in accordance with 60601-1, the requirements listed in EN 60601-1-1 shall apply. Not suitable for examining potentially infectious specimens. This type of instrument may be operated by trained laboratory personnel only.
IVD
The above-named microscopes comply with the Council Directive 98/79/EC
concerning in vitro diagnostics.
· The manufacturer assumes no liability for damage caused by, or any risks
arising from, using the microscope for
Leica M125 B nameplate
Leica M165 C B nameplate
Leica M series
User Manual
3
General Notes
Use in clean rooms The Leica M series can be used in clean rooms without any
problems.
Cleaning Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
Never use chemicals to clean colored surfaces or accessories with rubberized
parts. This could damage the surfaces, and products could be contaminated by
abraded particles.
The cleaning of glass surfaces and objectives in particular should be carried
out exclusively as outlined in the brochure “Cleaning of Microscope Optics”.
The information can be downloaded at: http://www.leica-
microsystems.com/products/ . Select your product and go to the “Download”
page.
For additional information, refer to page 56.
Servicing Repairs may only be carried out by Leica
Microsystems-trained service technicians. Only original Leica Microsystems
spare parts may be used.
Responsibilities of person in charge of instrument Ensure that the Leica
stereomicroscope
is operated, maintained and repaired by authorized and trained personnel only.
In most cases, we can provide special solutions on request. Some products can be modified, and we can offer other accessories for use in clean rooms.
Leica M series
User Manual
4
Important Safety Notes
User Manual This User Manual describes the special functions of the individual
modules of the Leica M stereomicroscopy series and contains important
instructions for their operational safety, maintenance, and accessories.
You can combine individual system articles with articles from external
suppliers (e.g. cold light sources, etc.). Please read the User Manual and the
safety instructions from the supplier.
Before installing, operating or using the instruments, read the user manuals
listed above. In particular, please follow all safety instructions.
To maintain the unit in its original condition and to ensure safe operation,
the user must follow the instructions and warnings contained in these user
manuals.
We guarantee the quality of our products. Our guarantee covers all faults in materials and manufacture. It does not, however, cover damage resulting from careless or improper handling.
Leica M series
User Manual
5
Symbols Used
Warning! Safety hazard!
· This symbol indicates especially important information that is mandatory to
read and observe.
Danger due to hot surface. This symbol warns against touching hot surfaces, e.g. those of light bulbs.
Disposal Notes on how to dispose on the microscope, its components and expend-
ables.
Failure to comply can cause the following: Hazards to personnel Functional
disturbances or damaged
instruments
Important information This symbol indicates additional information or
explanations that are intended
to provide clarity.
China RoHS 50 year EFUP (Environmentally friendly use period)
Warning of hazardous electrical voltage This symbol indicates especially
important information that is mandatory to
read and observe.
Failure to comply can cause the following: Hazards to personnel Functional
disturbances or damaged
instruments
Explanatory notes This symbol within the text stands for addi-
tional information and explanations.
Figures (1) Numbers in parentheses within the
descriptions relate to the figures and the items within those figures.
IVD labeling
I IVD nstrument for in vitro diagnostics.
IVD manufacturing date, for example 11 / 2011 for MM/YYYY November 2011.
Leica M series
User Manual
6
Safety Instructions
Description The individual modules fulfill the highest
requirements for observation and documentation of Leica stereomicroscopes of
the M series.
Intended use of instrument Leica Microsystems microscopes are opti-
cal instruments for improving the visibility of objects or specimens through
magnification. Accessories such as optical accessories, stands, illumination,
cameras etc. supplement the equipment configuration.
Non-intended use Using the instrument in any way contrary
to the specifications in the User Manual can lead to bodily harm and damage to
objects. Never use microscopes for in vivo examinations or eye surgery if they
are not expressly intended for such use. Never install any other plug or
unscrew optical systems
and mechanical parts unless expressly instructed to do so in the instructions.
The instruments and accessories described in this User Manual have been tested
for safety and potential hazards. The responsible Leica affiliate must be
consulted whenever the instrument is altered, modified or used in conjunction
with non-Leica components that are outside of the scope of this manual!
Unauthorized alterations to the instrument or noncompliant use shall void all
rights to any warranty claims as well as product liability and the Declaration
of Conformity.
Place of use Only use the instruments in closed, dust
free rooms and between +10 °C and +40 °C. Protect the devices from oil,
chemicals and extreme humidity. If using the devices outdoors, protect them
from dust and moisture. Never use electrical devices outdoors.
Electrical components must be placed at least 10 cm away from the wall and
away from flammable substances.
Avoid large temperature fluctuations, direct sunlight and vibrations. These
conditions can distort micrographic images, for example.
In warm and warm-damp climatic zones, the individual components require
special care in order to prevent the build-up of fungus.
Leica M series
User Manual
7
Safety Instructions (continued)
Responsibilities of person in charge of instrument These Safety Instructions
must be available
at the workplace.
Ensure that: The M series stereomicroscopes and
accessories are operated, maintained and repaired by authorized and trained
personnel only.
All operators have read, understood and observe this User Manual, and
particularly the safety regulations.
Repairs, service work Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare parts may be used.
Before opening the instruments, switch off the power and unplug the power
cable.
Avoid contact with powered electrical circuits, which can lead to injury.
Transport Use the original packaging for shipping or
transporting the individual modules of the Leica M stereomicroscopy series and
the accessory components.
In order to prevent damage from vibrations, disassemble all moving parts that
(according to the user manual) can be assembled and disassembled by the
customer and pack them separately.
Integration in third-party products When installing Leica products into third-
party products, the manufacturer of the complete system or its dealer is
responsible for following all applicable safety instructions, laws and
guidelines.
Disposal Once the product has reached the end of its
service life, please contact Leica Service or Sales about disposal.
Please observe and comply with the national and federal laws and regulations
that are equivalent to EC directives such as WEEE.
Like all electronic devices, the microscope, its accessory components and
consumables must never be disposed of with general household waste.
Leica M series
User Manual
8
Safety Instructions (continued)
Legal regulations Observe the generally applicable statu-
tory and country-specific regulations for accident prevention and
environmental protection.
EC Declaration of Conformity Electrically operated accessories are
constructed based on the state of the art of technology and are provided with
an EC Declaration of Conformity. See page 55
Health risks Workplaces with stereomicroscopes facilitate and improve the
viewing task, but they also impose high demands on the eyes and holding
muscles of the user. Depending on the duration of uninterrupted work,
asthenopia and musculoskeletal problems may occur. For this reason,
appropriate measures for reduction of the workload must be taken: Optimal
arrangement of workplace, work
assignments and work flow (changing tasks frequently). Thorough training of
the personnel, giving consideration to ergonomic and organizational aspects.
The ergonomic design and construction of the Leica M stereomicroscopy series
are intended to reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential transmission path for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using personal eyepieces for each
individual or detachable eyecups.
Careful handling Exercise particular care when setting up
the instruments. If it is specified that two or more people are required for
setup, compliance with this is mandatory.
Never spill any liquids on electrical instruments. This could cause the
stereomicroscope and other equipment to become electrically live and damage
people and instruments.
Never clean instruments using corrosive cleaning agents or those containing
acetone. For detailed information about care, refer to the User Manual for the
instrument.
Check the power cables regularly. Defective power cables can cause injuries.
Wait for bulbs to cool off before changing them. Touching hot bulbs can cause
burns.
Leica M series
User Manual
9
Safety Instructions (continued)
Light sources: safety regulations Light sources pose a potential risk from
glare. Therefore, lamps have to be operated in closed housings and in
installed condition.
External power supply for the focusing column (Leica M165 C B) Permitted power supply: SINPRO SPU46-110
Never look directly into the beam path (blinding hazard).
Do not select a white, strongly reflective background for the specimen.
Specifications: Input: 100-240 V AC
47-63 Hz 1.2 A Output: 33 V DC 1.51 A For indoor use only.
External power supply for TL5000 Ergo Permitted power supply: SINPRO
SPU130-110
Specifications: Input: 100-240 V AC
47-63 Hz 3.2 A Output: 33 V DC 3.93 A max. 130 W For indoor use only.
· Use only the power supply specified above. Other power supplies must not be
used. If the original power supply fails or is damaged, it must be replaced.
Repair is not permitted. Original power supplies are available from your Leica
branch office or Leica dealer.
Leica M series
User Manual
10
Contents
Copyright
2 Quick Start Guide
Function of the Microscopes
3 The Fastest Route to Success
29
General Notes
4 Overview of an M Series Microscope
30
Important Safety Notes
5 The correct interpupillary distance
31
Symbols Used
6 Using the Eyepieces
32
Safety Instructions
7 Focusing
33
Adjusting the Resistance of the Focus Drive
34
The Leica M Series
Changing the Magnification (Zoom)
35
Congratulations!
14 Click Stops and Magnifications
36
A Step Towards Infinity
15 Parfocality: More Comfort and Convenience for Your Work
37
The Electronics: Comfort, Convenience and Safety for your Experiments Iris Diaphragm
38
16
Maximum Compatibility
17 Eyepieces
On We Go
18 Magnification Factor of the Eyepieces
40
Health Notes
41
Assembly
Dioptric Correction
42
Base and Focusing Column
20 Dioptric Correction and Parfocality
43
Optics carrier
21 Graticules
44
Tube
22
Eyepieces
23 Objectives
and
Objective
24
TL5000 Ergo transmitted light base
25
Wiring: Connections (Leica M165 C B)
26
Cables: Cable Duct
27
Leica M series
User Manual
11
Optical Accessories
The Different Types of Objectives
46
Bases
Leica TL5000 Ergo Transmitted Light Base: Controls
48
Leica TL5000 Ergo Transmitted Light Base: Operation
49
Technical Data
Leica TL5000 Ergo Transmitted Light Base
52
Appendix
Calculating the Total Magnification and Field of View Diameter
54
Care, Maintenance, Contact Persons
55
Leica M series
User Manual
12
The Leica M Series
Leica M series
User Manual
13
Congratulations!
Congratulations on obtaining your new Leica M series stereomicroscope. We are
convinced it will exceed your expectations, as never before have we applied
our decades of experience in the areas of optics, mechanical engineering and
ergonomics in such an uncompromising manner.
The Leica M series embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the M series adapts
perfectly to your needs–no matter which accessories you require for your
tasks.
Though the reliability and robustness of Leica stereomicroscopes is legendary,
like any high-tech product, the Leica M series requires a certain degree of
care and attention. Therefore, we recommend that you read this manual. It
contains all the information you need regarding operation, safety and
maintenance. Simply observing a few guidelines will ensure that even after
years of intensive use, your stereomicroscope will continue to work as
smoothly and reliably as on the very first day.
We wish you the best of success in your work– after all, you are now equipped
with the best tool!
Leica M series
User Manual
14
A Step Towards Infinity
Ever since their introduction by Horatio S. Greenough, stereomicroscopes have
worked according to the optical principles based primarily on Ernst Abbe’s
research. For over a century, ingenious optics designers and engineers have
worked to push magnification, resolution and image fidelity to the limit
permitted by optics.
In doing so, they have always been constrained by the interrelation between
three factors: the higher a microscope’s resolution, the lower the available
working distance. If one increases the distance of the optical axes, the
three-dimensional image seen by the observer becomes distorted– a sphere
becomes an ellipse, a flat surface curves towards the observer.
Leica M series
User Manual
15
The Electronics: Comfort, Convenience and Safety for your Experiments
Never before have electronics been used as extensively in a Leica series as in the new M series lineup. Optics carrier, tripod, base and illuminator are all connected using electrical contacts–which provides a number of advantages.
Fewer cables A large part of the cables have been routed in the interior of the column. Data are also transferred via the interface between the column, the optics carrier and the carrier. The direct result is that you need fewer cables–this not only makes your workstation neater and more comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply the power.
Leica M series
User Manual
16
Maximum Compatibility
Leica engineers were careful to ensure that the new Leica M series–like its
predecessors– remains compatible with existing series. This means that
objectives, bases, tubes and so on can be reused.
Objectives All new objectives of the Leica M series are parfocal.
If you prefer, you can continue to use the previous Leica objective series. In
this case, parfocality is no longer guaranteed.
Tubes The interface between the optics carrier and the tube has remained the
same, so existing tubes fit the new M series. The new tubes are designed for
eyepieces with field number 23, while the predecessor models were only
designed for field number 21, resulting in a smaller object field.
Eyepieces The new M series eyepieces have an audible and tangible click to
provide immediate feedback in case of accidental adjustment.
Leica M series
User Manual
17
On We Go
If your new Leica microscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on page 28.
If, on the other hand, you are assembling the microscope yourself, continue with the “Assembly” chapter, which begins on page 19.
Leica M series
User Manual
18
Assembly
Leica M series
User Manual
19
Base and Focusing Column
The first step is to connect the focusing column of the M series to the
corresponding base.
Tools used Allen key, 3 mm
Assembling the column adapter 1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive 2. Securely screw the focusing column to the
base using the six included screws.
Leica M series
User Manual
20
Optics carrier
Tools used Allen key, 4 mm
Assembling the Optics Carrier 1. Place the optics carrier on the focus-
ing column so that the screw fits into the thread provided and the lug fits
into the groove.
2. Press the optics carrier backwards to the focusing column and screw it in place using your other hand.
Leica M series
User Manual
21
Tube
All intermediate tubes that fit between the optics carrier and the binocular
tube are fitted in the same manner.
Tools used No tools required.
Assembling the tube 2. Push the tube (for example, the inclined
binocular tube) into the dovetail ring and rotate it slightly in both
directions until the positioning screw meshes with the guide groove.
3. While holding the tube only slightly, carefully tighten the positioning screw. It is automatically brought to the correct position.
Preparations 1. Unscrew the positioning screw and remove
the protective cover.
Leica M series
User Manual
22
Eyepieces
Tools used No tools required.
Magnification range You can extend the overall magnification range using
available 10×, 16×, 25× and 40× widefield eyepieces for persons wearing
glasses.
Preparation 1. If you want to use an optional graticule,
insert it now (page 44).
2. Remove the plastic tube guard.
Inserting the eyepieces 3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they fit tightly and accurately.
4. Securely tighten the clamping screws.
Leica M series
User Manual
23
Objective
Tools used No tools required.
Preparation 1. Remove the protective cap on the optics
carrier by turning it.
Attaching the objective
· Hold the objective firmly during assembly and disassembly so that it does
not fall onto the stage plate. This applies particularly to the 2x
planapochromatic objective, which is very heavy. Remove all specimens from the
stage plate first.
2. Screw the objective clockwise into the optics carrier.
Leica M series
User Manual
24
TL5000 Ergo transmitted light base
Unpacking the base The base is delivered with the adapter plate installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and non-slip surface.
Focusing drive and column 1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column to the bottom using the 4 Allen screws.
3. Reattach the adapter plate to its original position using the 6 Allen
screws.
Refer also to the separately provided User Manual for the TL5000 Ergo transmitted light base.
Leica M series
User Manual
25
Wiring: Connections (Leica M165 C B)
The new Leica M series features extensive encoding with which various microscope data and settings can be read out and reproduced later.
The terminals
The interface to the optics carrier is on the column.
The connection to other instruments is made using the terminals on the rear side of the column:
1
1. 2×CTL2 terminals for auxiliary equipment
such as the ring illuminator, the base and
other accessories from the Leica product
range.
2 2. Terminal for the 50-watt power supply
provided.
Leica M series
User Manual
26
Cables: Cable Duct
The integrated cable duct in the column enables a neat cable layout around the microscope. For example, the USB or FireWire cables of the camera can be stowed in the cable duct.
Feeding the cables 1. Unscrew the three screws on the cable
duct.
3. Place the cables in the cable duct and screw the cover on tightly.
2. Remove the cover of the cable duct.
Tip: Estimate the length of the cable ends you will need before screwing on the cover. For thick cables, it is difficult to change the length retroactively.
Leica M series
User Manual
27
Quick Start Guide
Leica M series
User Manual
28
The Fastest Route to Success
Your Leica stereomicroscope has been delivered in completely assembled
condition by your Leica partner, and naturally you want to get right to work.
Therefore, your next step should be to study the Quick Start Guide, which
outlines the most important steps at a glance. This manual will then
familiarize you with the finer details of your microscope. The following pages
contain important, practical information that makes using it every day easier.
Take time to read it it’s worth it!
The M series Quick Start Guide (included with instrument).
Leica M series
User Manual
29
Overview of an M Series Microscope
1 Switch click stops on/off
2 Magnification changer (zoom)
3 Coarse/fine focusing
4 Transmitted light base
5 Interchangeable objective
6 Built-in iris diaphragm
7 Fastening screw for the binocular tube (or accessories)
8 Adjustable eyepiece tubes
1
9 Fastening screws for the eyepieces
2
10 Eyepieces for spectacle wearers with dioptric correction
3
and eyecups
11 Trinocular tube
Leica M series
User Manual
11
10
9 8 7
6
5
4
30
The correct interpupillary distance
The interpupillary distance is correctly set if you see a single circular
image field when looking at a specimen.
If you are still a novice microscope user, you may need a short time to become
accustomed to this. Not to worry–after a little while, it will become
automatic.
Adjusting the interpupillary distance 1. Look into the eyepieces.
2. Hold the eyepieces with both hands. Push the eyepieces together or
separate them until you see a circular image.
3. Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting.
Reference values The distance between eye and eyepiece measures approx. 22 mm for 10/23B widefield eyepieces for persons wearing glasses.
Leica M series
User Manual
31
Using the Eyepieces
The eyepieces form the connection between the tube and the eye of the
observer. Simply push them into the tube and they are ready to use.
Each eyepiece offers a certain magnification factor that has a determinative
effect on the total magnification. Furthermore, all Leica eyepieces can be
equipped with practical graticules that enable measuring and quantifying of
specimens.
If you do not wear glasses: 1. Hold the eyepiece firmly and rotate the
eyecups forwards counterclockwise.
2. If an eyepiece is equipped with the inte-
If you wear glasses: 1. Hold the eyepiece firmly and rotate the
eyecups counterclockwise towards the rear, as otherwise the viewing distance
is too great.
Dioptric Correction A built-in dioptric correction is available for eyeglass wearers. For more information, refer to page 42
grated dioptric correction, turn the value to the “0” mark.
2. If an eyepiece is equipped with the integrated dioptric correction, turn the value to the “0” mark.
By the way, one benefit of viewing with eyeglasses is a drastically lower risk of bacterial transmission (see page 41). The soft material of the eyecup also ensures that your glasses will not be scratched, even if they contact the eyepiece.
Leica M series
User Manual
32
Focusing
Focusing raises or lowers the stereomicroscope using the focusing drive. The
specimen detail is brought into sharp focus as soon as it is in the focal
point of the objective.
The focusing drive can be operated either left- or right-handed.
Focusing The inner, coarse adjustment is used for
covering great distances.
The outer, fine adjustment is used for fine focusing.
Coarse/fine adjustment The sharpness is adjusted using the coarse/fine
adjustment.
· 2. The coarse/fine adjustment carries a load of up to 15 kg.
The resolution of the coarse/fine adjustment is 1 µm.
Leica M series
User Manual
33
Adjusting the Resistance of the Focus Drive
Adjusting the resistance Is the focusing drive too loose or too tight? Does
the equipment tend to slide downwards? The resistance can be adjusted
individually depending on the equipment weight and personal preferences as
follows:
1. Grip the outer drive knobs with both hands and turn them towards each
other until the desired resistance is reached during focusing.
Leica M series
User Manual
34
Changing the Magnification (Zoom)
All M series microscopes have an integrated zoom. The name indicates the zoom
range covered:
Leica M125 B = 12.5:1 Leica M165 C B = 16.5:1
The rotary knob for the zoom can be used either left or right-handed.
Zooming 1. Look into the eyepieces.
2. Focus on the specimen.
3. Rotate the magnification changer until the desired magnification is
configured.
Leica M series
User Manual
35
Click Stops and Magnifications
The zoom button can optionally be operated either with or without ratchet steps. Continuous zoom is possible when the click stops are disabled, which many users find convenient. On the other hand, when the click stops are enabled, photographs, measurement results etc. can be reproduced more accurately.
Enabling and disabling click stops 1. Push the top button downwards to disable
the ratchet steps.
2. Push the bottom button upwards to enable the ratchet steps.
Magnifications and fields of view The formula on page 54 provides additional information about the magnifications and field of view diameters, with consideration given to the position of the magnification changer and the eyepiece and objective combination used.
Leica M series
User Manual
36
Parfocality: More Comfort and Convenience for Your Work
All Leica stereomicroscopes are parfocally matched, meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus. The focus needs to be readjusted only if you want to view a specimen detail that is located higher or lower.
Parfocality 1. Enlarge the view to the maximum level.
2. Focus on the specimen.
You are done! Even if you select a smaller working distance, the specimen
remains pin-sharp.
Requirements for parfocal work If you are using an eyepiece with dioptric
correction, the procedure differs from this description. For more information,
refer to page 43
The parfocality is maintained until you focus on another level of the specimen.
Leica M series
User Manual
37
Iris Diaphragm
The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter: it regulates the available light, which changes the depth of field. The “depth of field” (or “depth of focus”) is the area of a specimen that is brought into sharp focus.
Closing the iris diaphragm Close the iris diaphragm by turning the
knob to the left. The subject appears darker and the depth of field increases.
Opening the Iris Diaphragm Open the iris diaphragm by turning the
knob to the right. The subject now appears brighter, but the depth of field
decreases.
Leica M series
User Manual
38
Eyepieces
Leica M series
User Manual
39
Magnification Factor of the Eyepieces
An eyepiece not only makes it possible to look passively into the microscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10x and 40x.
The following eyepieces are available for the M series:
Magnification Dioptric Correction
Order number
10×
± 5 diopter settings
10 450 023
16×
± 5 diopter settings
10 450 024
25×
± 5 diopter settings
10 450 025
40×
± 5 diopter settings
10 450 026
Leica M series
User Manual
40
Health Notes
Potential sources of infection
· Direct contact with eyepieces is a potential transmission method for
bacterial and viral infections of the eye. The risk can be kept to a minimum
by using individual eyepieces or detachable eyecups. Eyecups can be ordered
separately. Please contact your Leica partner.
Separate eyecups are an effective way of preventing infections.
Leica M series
User Manual
41
Dioptric Correction
All Leica eyepieces are also available with builtin dioptric correction, allowing the microscope to be used without glasses even by those with vision problems. The correction comprises ±5 diopter settings.
Using the Dioptric Correction 1. Set the dioptric correction of both
eyepieces to the mid position (“0” diopter settings).
2. While wearing your glasses, look through the eyepieces and focus on the
specimen.
Note that when using dioptric correction, the advantage of parfocality is lost – thus you have to manually refocus each time you change the zoom level. To also use parfocality with dioptric correction, refer to the instructions on page 43
3. Rotate both eyepieces to the maximum value of “+5”.
4. Hold one eye closed and rotate the other eyepiece in the “-” direction until the specimen appears sharp.
5. Then, open the other eye and correct the diopter settings until the image is uniformly sharp.
Leica M series
User Manual
42
Dioptric Correction and Parfocality
Leica stereomicroscopes are parfocally matched. The prerequisite for this is
the correct setting of the diopters and the parfocality. The following
adjustments have to be carried out only once by each user.
Preparations Move the lever of the video/phototube to
the “observation” position and open the diaphragm.
Adjusting 1. Set the dioptric correction for both
eyepieces to “0”.
2. Select the lowest magnification and focus on a flat specimen.
3. Select the highest magnification and readjust the sharpness.
4. Select the lowest magnification again, but do not look into the eyepieces.
6. Rotate the eyepieces counterclockwise in the “+” direction as far as they
will go (+5 diopter settings).
7. Look into the eyepieces.
8. Slowly rotate each eyepiece individually in the “” direction until each
eye sees the object sharply imaged.
9. Select the highest magnification and refocus if necessary.
Now, if you adjust the magnification from the lowest to the highest level, the
specimen is always brought into sharp focus. If not, repeat the process.
Leica M series
User Manual
43
Graticules
Use Leica graticules make counting easier, particularly for workstations that are not equipped with a digital camera.
2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the graticule fits tightly.
4. You can now align the graticule by rotating the eyepiece in the tube and then tightening it using the clamping screw.
The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces.
1. Screw the insert off of the eyepiece.
3. Screw the insert and graticule firmly into place and replace the eyepiece in the tube.
Leica M series
User Manual
44
Objectives and Optical Accessories
Leica M series
User Manual
45
The Different Types of Objectives
To meet the various requirements regarding imaging properties, there is a
choice of highquality interchangeable planachromatic and planapochromatic
objectives and also lowerpriced interchangeable achromatic objectives.
Achromatic objectives are particularly suited for specimens with high-contrast
structures.
Flat-field (planachromatic) objectives are particularly well suited for
studying flat objects such as wafers and thin sections.
Achromatic objectives The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2× achromatic
objectives offer countless variants for selecting the object field diameter,
magnification ranges and working distances (see page 54).
Planachromatic objective 1× For the highest requirements for overall image
quality, we recommend equipping the microscope with the 1× plan (flat-field)
objective, which returns sharp, contrast-rich object fields all the way to the
border.
Achromatic objectives with a long focal length For special applications, achromatic objectives with long working distances and focal lengths of f=100 mm to 400 mm are available.
With planapochromatic objectives, the finest structures are visible with high contrast. The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity.
Leica M series
User Manual
46
Bases
Leica M series
User Manual
47
Leica TL5000 Ergo Transmitted Light Base: Controls
12 Rear side of the TL5000 Ergo
1 2 3 45 Left side of the TL5000 Ergo
1 Power switch for the base 2 Power supply connection
Refer also to the separately provided User Manual for the TL5000 Ergo
transmitted light base.
1 LED display for opening and positioning the aperture or for balance in DF
mode.
2 Control for the size of the aperture/ balance
3 “BF” button for bright field (press and hold for 2 seconds to (de)activate
the automatic aperture)
4 “RC” button for Rottermann Contrast / in the RC mode, press and hold the
button to define the aperture size
5 “DF” button for dark field
1
2
Right side of the TL5000 Ergo
1 On/Off switch for the light source / Press and hold for 5 seconds to reset
the base to factory default settings
2 Controls for controlling the brightness intensity
Leica M series
User Manual
48
Leica TL5000 Ergo Transmitted Light Base: Operation
The transmitted light base must only be connected to a grounded socket with a faultless power cable! Failure to observe these warnings may result in serious personal injury or even death!
Switching the transmitted light base on and off 1. Switch on the transmitted
light base with
the power switch on the rear side.
2. Press the on/off switch on the right side once to turn on the illumination.
· The LED illumination can be very bright! Check and adjust the intensity of the illumination to a suitable brightness before looking through the eyepieces.
Continued on next page.
Leica M series
User Manual
49
Leica TL5000 Ergo Transmitted Light Base: Operation (Continued)
3. Look through the eyepiece and adjust the light intensity using the control on the right side. The intensity of the illumination is visualized by the LED scale.
The following assumes that the power switch on the rear side of the device is always switched on. This switch is not mentioned in the remaining part of this User Manual.
4 Press the on/off switch on the right side once again to turn off the illumination on the base.
Leica M series
User Manual
50
Technical Data
Leica M series
User Manual
51
Leica TL5000 Ergo Transmitted Light Base
Light source Light source Illuminated field: Bright field Illuminated field: Dark field Relief Contrast System (RCTM) Internal shutter/lamp control Integrated filter holder Matching of high num. aperture Remote control options AntiShockTM Pads Dimensions (W×H×D) Power supply Input Output Connections Power supply
LED 65 mm 40 mm Yes Yes Yes Yes Yes Yes 412×341×46 mm
100240 VAC 47 63 Hz 3.2 A 33 VDC 3.93 A 130 W MAX
1
Leica M series
User Manual
52
Appendix
Leica M series
User Manual
53
Calculating the Total Magnification and Field of View Diameter
Parameter
MO Magnification of objective
ME Magnification of eyepiece
z
Magnification changer position
q
Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45°
ErgoTubeTM
r
Factor 1.25× if the planachromatic and planapochromatic
objectives of the MZ125/MZ16 are used on the MS5, MZ6,
MZ75 or MZ95
NFOV Field number of the eyepiece. Field numbers are printed on the eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.
Example
MO 1× objective
ME 25×/9.5 eyepiece
z
Zoom position 4
q
Coaxial incident light 1.5×, tube factor
r
Factor 1.25×
Calculation example: magnification in the binocular tube
MTOT VIS = MO × ME × z × q × r or
1 × 25 × 4 × 1.5 × 1.25 = 187.5×
Calculation example: field of view diameter in the specimen
OF:
NFOV MO × z ×q ×r
Leica M series
User Manual
54
Care, Maintenance, Contact Persons
We hope you enjoy using your high-performance microscope. Leica microscopes
are renowned for their robustness and long service life. Observing the
following care and cleaning tips will ensure that even after years and
decades, your Leica microscope will continue to work as well as it did on the
very first day.
Warranty benefits The guarantee covers all faults in materials and
manufacture. It does not, however, cover damage resulting from careless or
improper handling.
EC Declaration of Conformity To download the EC Declaration of Conformity, use
this link
http://www.leica-microsystems.com/ products/stereo-microscopes-macroscopes/
research-manual/
Select the microscope type and go to the “Download” page.
Care Protect your microscope from moisture,
fumes and acids and from alkaline, caustic and corrosive materials and keep
chemicals away from the instruments.
Plugs, optical systems and mechanical parts must not be disassembled or
replaced, unless doing so is specifically permitted and described in this
manual.
Protect your microscope from oil and grease.
Do not grease guide surfaces or mechanical parts.
Leica M series
User Manual
55
Care, Maintenance, Contact Persons (continued)
Protection from dirt Dust and dirt will affect the quality of your results.
Put a dust cover over the microscope when it will not be used for a long time.
Use dust caps to protect tube openings, tubes without eyepieces, and
eyepieces.
Cleaning polymer components Some components are made of polymer or are polymer-coated. They are, therefore, pleasant and convenient to handle. The use of unsuitable cleaning agents and techniques can damage polymers.
Permitted measures Clean the microscope (or parts of it)
using warm soapy water, then wipe using distilled water.
For stubborn dirt, you can also use ethanol (industrial alcohol) or
isopropanol. When doing so, follow the corresponding safety regulations.
Keep accessories in a dust-free place when not in use.
Remove dust with a bellows and a soft paintbrush.
Clean eyepieces and objectives with special optics cleaning cloths and pure alcohol.
Leica M series
User Manual
56
www.leica-microsystems.com
Copyright © Leica Microsystems CMS GmbH · Ernst-Leitz-Straße · 35578 Wetzlar
Germany 2013 · Phone +49 6441-29-0 · Fax +49 6441-29-2599
LEICA and the Leica logos are registered trademarks of Leica IR GmbH. Printed
on chlorine-free bleached paper. III/13/M.H. Revision 1.0, issued March 14th,
2013
References
- Microscopes and Imaging Systems | Leica Microsystems
- Microscopes and Imaging Systems | Leica Microsystems
- Manual-Hub.com – Free PDF manuals!
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>