MECAN MBP01-XZ2 Bio Pyramid User Manual

MECAN MBP01-XZ2 Bio Pyramid

Standard Accessories

Camera Installation

Camera

Please attach in following order

1. Attach CLA‐12 to XZ‐2 lens part
2. Attach RA5255B to CLA‐12
3. Attach Φ52mm sleeve to RA5255B
4. Attach all of above to hood body

Filter

When using a glass filter, install onto φ52mm sleeve. For gelatin filters, slide it into the filter tray.
** 75mm square size can be used.

Accessories

Please connect to camera when you use AC adapter or remote cable.

• AC adapter and remote cable cannot be used simultaneously

Instruction

mark is important

Following XZ‐2 User’s Guide, set your camera as described below.
Gel documentation takes place in a darkroom environment and it is difficult to capture quality images with full automatic mode. Please select M (manual) mode for documentation.
Followings are setting instruction on manual mode.

Camera Setting (1)

(This color indicate corresponding page in XZ‐2 user guide )

1. Select M (manual) mode by using Mode Dial

** Manual P18

Image Stabilizer

Press OK button and display live control. Select the image stabilizer item using ▲▼.
Select an“IS1”with ◄► and press OK button.
** Manual P36

Monotone (black and white)

Press OK button and display live control. Select the picture mode item using ▲▼.
Select an“ Monotone” with ◄► and press OK button.
** Manual P37

ISO Sensitivity ★

Press OK button and display live control. Select the ISO sensitivity item using ▲▼.
Select an“1600”with ◄► and press OK button.
** Manual P44

Flash Off

Keep strobe inside the camera and flash won’t go off

Camera Setting (2)

adjusting aperture and shutter speed ★

1. Setting aperture

Press ▲ on sub dial and display ◄► mark on screen. Select an“F8.0”F value with sub dial ◄►.
** Manual P18

1. Press ▲
2. adjust with ◄►

Setting shutter speed

Press ▲ on sub dial and display ▲▼ mark. Select an shutter speed “2 (1/2) sec”with sub dial
◄►. The shutter speed can be set to minimum 1/2000 sec to maximum 16 minutes [BULB].
** Manual P18

1. Press ▲
2. adjust with ▲▼

Camera Setting (3)

** Manual P46

1. Long Exposure Noise Reduction

Press MENU button and select a “Custom Menu “with sub dial ▼ and press OK button.
Select an “Expo/Metering/ISO“ with sub dial ▼ and press OK button.
Select a “Noise Redact“ with sub dial ▼, and set it ON and press OK button.
** Manual P61

High ISO Noise Reduction

② High ISO Noise Reduction
Press MENU button and select a “Custom Menu “with sub dial ▼ and press OK button.
Select an “Expo/Metering/ISO“ with sub dial ▼ and press OK button.
Select a “Noise Filter“ with sub dial ▼, and set it Standard and press OK button.
** Manual P61

Sleep Setting

Press MENU button and select a “Custom Menu “with sub dial ▼ and press OK button.
Select an “Display“ with sub dial ▼ and press OK button.
Select a “Sleep“ with sub dial ▼, and set it OFF and press OK button.
** Manual P60

Custom mode Settings (Restore above preselected settings)

Press MENU button and select a “Shooting Menu 1“ with sub dial ► and press OK button.
Select an “Reset/C Mode Setup “ with sub dial ▼ and press OK button.
From the menu, select a “Custom Mode 1“ with sub dial ▼, and press OK button.
Settings are registered to Mode dial “C 1“
** Manual P48

Meme on battery charging and remote control

1. AC adapter［F‐3AC］and remote cable［RM‐UC1］has same connector shape. Therefore, camera
   can be operated while using［F‐3AC］but unable to use［RM‐UC1］simultaneously.

2. Please fully charge battery before connecting remote cable to camera.

Setting Up Transilluminator

1. Place focusing sheet on the Transilluminator.

2. Place Hood on the ①
   ⇒ Place Hood on the Transilluminator after installing camera.
   ⇒ place Hood in a way that it covers entire emission surface.

3. Focusing sheet will become luminous once Transilluminator is turned on.
   **image is MBP‐UVCR

Camera Focus and Zoom Setting ★

Focusing

Choosing focus target
Press sub dial ◄ and display the AF target.
Select the AF target to which you want to focus on with sub dial and press OK button.
** Manual P26

⇒ Focus Lock setting.

Press OK button and set focus with sub dial ▲▼.
Select “S‐AF“ with sub dial ►, and press OK button.
** Manual P14

⇒ Keeping the shutter button pressed halfway, and make sure AF target position.
⇒ Adjust zoom with Zoom Lever as it requires.

MF (Manual Focus) Setting
⇒ Switching to MF after procedure

1. would fix focus position.

⇒ Press OK button and change focus setting with sub dial ▲▼.
Set MF with sub dial ► and press OK button. Readjust with sub dial when out of focus.
Press and hold OK button for a while changes adjusting of F value and manual focus.

Gel Documentation

Turn on the Camera

⇒ Place the gel right in center and turn on the Transilluminator.
⇒ Turning the zoom lever to the right and adjusts to appropriate shooting range.
** Manual P10

Adjusting Brightness ★

Adjusting brightness with“ Shutter Speed”

⇒ Shutter speed means exposure time. Slow shutter speeds let more amounts of light reaches
the image sensor and thus are used to brighten images. Please adjust accordingly.
(Tip: start with rough estimates and narrow a target down for precise adjustment)
** Manual P18

Manual Focus

When it is difficult to focus with AF (e.g. gel is too thick, or unable to use focusing sheet), use manual focus instead.

1. Press OK button and display the Live Control, and select the MF mode.
2. Adjust focusing position with sub dial

** Manual P43

Transfer Picture data to PC.

You can transfer images to a computer by connecting the camera to the computer with the provided USB cable and software (CD‐ROM).,
** Manual P74

Saving Image / Viewing Photograph / Erasing / Printing

1. Captured images will be save in SD card ** Manual P83
2. To see captured images, please select picture from［playback mode］** Manual P11
3. To find images from the list (Index display) ** Manual P11
4. To enlarge images (Close‐up playback) ** Manual P11
5. Erasing Images ** Manual P12
6. Printing Images ** Manual P70

Other settings … Please refer XZ‐2 Instruction manual for detail.
The guide is one of the standard settings for documentation and for reference purpose only.
Please adjust details in accordance with your particular observing environment.
White Balance – When you shoot with color, please adjust white balance in accordance with color tone of light source. You will be able to capture image without any color bias. ** Manual P38
Image Size – Setting image (pixel) size. Smaller data (pixel) size is easy to handle.
Large data (pixel) size is preferable for printing and enlarging. Manual P62
Date – Able to verify captured date. ** Manual**  P28

Large size observing window (with UV shield filter)
UV transmittance is less than 0.1％.
It eliminates most UV rays.
★ Even with the shield, there is still a small amount of transmittance.
Therefore, please avoid observing continuously for long time.

**Photo Printer * sold separately ‐ Epson Picture Mate PM225 etc.
Able to print images in just 3 easy steps. manual P71

• operation may vary depending on the model. Please refer to manual of your printer model
• Image on the right is BP‐UVOR.

Filters:

The 3 sharp‐cut filters cut excitation wave length and transmit only emission wavelength. SC‐58 cuts wavelength below 580nm and SC‐60 cuts below 600nm. These can be used for EtBr. ALSC‐56 cuts wavelength below 560nm, and can be used for SYBR Green/SYPRO Orange etc. Please switch filters for appropriate regent. Also, any other filters with size 75mm square can be set onto filter‐tray.

Bio-Pyramid : Specification

◆  Maximum shooting area| 265mm×205mm（= interior dimension

• May vary, depends on the camera.
  ◆  Shooting range

Include φ52mm sleeve With super mount

| 310mm 350mm～360mm
★NOTICE Please select the camera that can be
focused to shooting area within these ranges.
◆  Filter     Glass filter

Gelatin filter

| Use φ52mm
Use 3 inch square filter
◆   Viewer window UV shield filter

Transmittance range (220~450nm)

| MAX 0.09%    Average 0.02%
(measured, not guaranteed number)
◆  Size| W287 ×D227mm（=Hood External Dimensions） H 310mm (include camera mounting part)
H 415mm (include super-mount)
◆  Weight Body/Super-mount| 1040g / 200g

References

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