GE Healthcare INV-907 Motor Valve Instructions

GE Healthcare INV-907 Motor Valve

Product Information

Specifications

• Product Name: Valve INV-907
• Manufacturer: GE Healthcare
• Compliance: CE Certified
• Product Class: Class A

FAQ

• What is the purpose of Valve INV-907?
• The Valve INV-907 can be used for various purposes, including loading a sample loop, injecting the sample onto the column, washing the system pump, and switching between upflow and downflow in a column.
• How should I ensure my safety when using hazardous chemicals with Valve INV-907?
• When using hazardous chemicals, it is important to take suitable protective measures, such as wearing protective glasses and gloves resistant to the chemicals used. Users should also follow local regulations and instructions for safe operation and maintenance of the system.
• How should I install Valve INV-907?
• Before connecting the Valve INV-907, make sure the power is switched OFF at the system pump or the complete system. Follow the installation instructions provided in the user manual, including connecting the valve with UniNet cables and connecting the tubing as per the guidelines mentioned.

Important user information

• All users must read this entire manual to fully understand the safe use of Valve INV-907.

WARNING!
The WARNING! sign highlights instructions that must be followed to avoid personal injury.
It is important not to proceed until all stated conditions are met and clearly understood.
CAUTION!
The Caution! sign highlights instructions that must be followed to avoid damage to the product or other equipment. It is important not to proceed until all stated conditions are met and clearly understood.
Note
The Note sign is used to indicate information important for trouble-free and optimal use of the product.

CE Certifying
This product meets the requirements of applicable CE directives. A copy of the corresponding Declaration of Conformity is available on request.
The CE symbol and corresponding declaration of conformity is valid for the instrument when it is:

• used as a stand-alone unit, or
• connected to other CE-marked GE Healthcare instruments, or
• connected to other products recommended or described in this manual, and
• used in the same state as it was delivered from GE Healthcare except for alterations described in this manual.

Recycling
This symbol indicates that the waste of electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment.

WARNING!
This is a Class A product. In a domestic environment, this product may cause radio interference in which case the user may be required to make adequate measures.

Introduction

Valve INV-907 is a motorised rotary 7–port valve. The valve is used in ÄKTA™design chromatography systems. It is powered by the ÄKTAdesign P-900 series system pump and is controlled by the UNICORN™ control system.
The valve has 3 positions used for:

• loading a sample loop
• injecting the sample into the column
• washing the system pump.

The valve can also be used to switch between upflow and downflow in a column.

Features

• A completely swept flow path minimises eluent or sample ”memory effect”.
• Flow rates up to 100 ml/min.
• All wetted parts are plastic PEEK.

Safety
WARNING! When using hazardous chemicals, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the chemicals used. Follow local regulations and instructions for safe operation and maintenance of the system.

Installation

CAUTION! Before connecting Valve INV-907 ensure the power is switched OFF at the system pump or the complete system.
Unpacking
Unpack the valve and check the items against the packing list. Inspect the items for obvious damage which may have occurred during transportation.
Installing the valve

1. Connect the valve with two UniNet cables as a part of the UniNet 2 chain.

2. Set the ID switch at the bottom to the required valve number 0–9. The number should correspond to that used in UNICORN.
   Note: All valves must have different numbers.

3. Mount the valve vertically, in the way it is shown in the user documentation of your ÄKTAdesign chromatography system.

4. Apply an identification number from the labels supplied. The number should be the same as that of the rotary switch at the bottom of the valve. Place the number so that it is easily readable from the front.

5. Connect the tubing.

6. When used as an injection valve connect the tubing as follows:
   Port Connect to

   • Column inlet
   • Sample loop or bottom tubing of Superloop
   • Injection port
   • Waste
   • Waste
   • Sample loop or top tubing of Superloop
   • Outlet of the pump

7. When used as an upflow/downflow valve, connect the valve as indicated in the user documentation of your ÄKTAdesign chromatography system.

Operation

The valve is controlled by UNICORN. When used as an injection valve use the instruction InjectionValve in System Control:Manual: Flowpath. The valve can be set in 3 positions:

• LOAD position 1 (home position)
• INJECT position 2
• WASTE position 3

If the valve has not been activated it is in position 1.

LOAD, position 1

INJECT, position 2

WASTE, position 3

Manual sample loading

• The sample loop and Superloop can be loaded with a syringe. Connect the Superloop with the bottom tubing connected to port 2 and the upper tubing to port 6.
• For large volumes use a luer female/1/16” male union connected to port 3.
• For smaller volumes use an injection needle together with the injection fill port as described below:
• Check the fill port nozzle is screwed into the fill port.

Note: The thread is left-handed.

1. Loosely thread the fill port screw into valve port 3.
2. Insert the injection needle (0.7 mm o.d.) into the injection fill port.
3. Tighten the fill port until the nozzle has formed a seal around the needle tip. When the seal is adjusted correctly, it feels as if you are penetrating a septum at the end of the injection fill port. The seal should provide easy insertion and removal of the needle.
4. Mount the syringe holder on the fill port.

Two techniques can be used for filling the sample loop; partial or complete filling.

Type of filling Volume to load

• Partial filling max 50% of the sample loop volume
• Complete filling 2–5 times the sample loop volume

Partial Filling
Partial filling is used when high recovery is required. Partial filling allows the injected volume to be changed without changing the loop and does not waste the sample.
Partial filling is achieved as follows:

1. Set the injection valve to position LOAD.

2. Load the syringe with a large volume of buffer (5× the loop volume).

3. Fill the sample loop carefully with buffer.

4. Set the injection valve to position INJECT before taking out the syringe.
   Note: If the syringe is taken out when the injection valve is in position
   LOAD, self-drainage will occur and air will enter the sample loop.

5. Load the syringe with the required volume of sample. No more than half (50%) a loop volume of the sample should be loaded into the loop.

6. Insert the syringe in position 3 on the injection valve. Set the injection valve to position LOAD.
   Note: Do not load the sample before the valve is in position LOAD.

7. Gently load the syringe contents into the loop.

8. Leave the syringe in position. The sample will be injected into the column when the valve is switched to INJECT in the method.

Complete filling

• In this method a surplus of sample is used to ensure that the sample loop is filled, allowing reproducible sample volumes. In preparative applications, the sample volume should be at least 2 times the volume of the sample loop. For analytical reproducibility a sample volume 5 times the volume of the sample loop should be used.
• With complete filling the sample volume can only be changed by changing the loop size.
• Complete filling is achieved as follows:

1. Set valve to position LOAD.
2. Load the syringe with the sample (2-5 times the loop volume).
3. Gently load the syringe contents into the loop.
4. Leave the syringe in position. The sample will be injected into the column when the valve is switched to INJECT in the method.

Note: If the syringe is taken out before the sample is injected into the column, self-drainage will occur and the loop will be emptied.
Emptying the sample loop
When emptying the sample loop a buffer volume of approx. 5 times the sample loop volume should be used to flush the loop and ensure that all sample is injected into the column.
Storage
Overnight: The valve can be left filled with a buffer.
Weekend or Long-term storage: Flush the valve with water and then fill it with 20% Ethanol.

Maintenance

WARNING! When using hazardous chemicals, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the chemicals used. Follow local regulations and instructions for safe operation and maintenance of the system.
CAUTION! Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the valve.

Period Action
Every 12 months Change channel plate and or when required distribution plate

Cleaning-in-place
Pump a cleaning or sanitizing agent through the valve. The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer.

Changing channel plate and distribution plate
WARNING! NaOH is injurious to health. Avoid spillage.
A replacement kit, Valve Kit INV–907, is available, see ordering information in Reference Information.

1. Ensure that the valve is in position 1 and then disconnect it from the pump.
2. Remove the 4 screws on the front using the supplied 3 mm Allen key. Loosen each one equally in turn so the distribution plate comes off parallel to the valve body.

• Slide the screws out.
• Remove the distribution plate containing the ports.
• Remove the old channel plate and insert a new one.
• Remount a new distribution plate so that text 3 is horizontal and to the right of the central tubing connection. Using the Allen key, tighten the 4 screws in turn, a little at a time, until the distribution plate is fixed to the valve body.

This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment.

Troubleshooting

If the suggested actions do not correct the fault, call GE Healthcare.

This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment.

Reference information

Description
The valve consists of two main parts:

• Housing which encloses the motor and electronics.
• Valve body with a rotating central core.

• As the channel plate is turned by the motor, different ports are connected.
• Valve switching is controlled from UNICORN by reading the actual position of the channel plate.
• The geometry of the valve assures that the flow path is completely swept so that the solvent or sample ”memory effect” is virtually non-existent.
• The valve rotates the shortest way to the next position (max. 180°). The material used in the switching parts ensures both long mechanical and chemical lifetimes.
• The valve housing contains no user-replaceable items.

Technical specifications

Operating data

• Max Flow rate: 100 ml/min
• Max Pressure: 25 MPa (250 bar, 3600 psi)
• Back pressure: <50 kPa at 100 ml/min with water
• Leakage: <0.1 μl/min at 25 MPa
• pH stability range: 1–13, 1–14 (< 1-day exposure)
• Viscosity: Max. 5 cP
• Switch time: <260 ms between two adjacent positions
• Operating lifetime: >50 000 cycles, two adjacent positions
• Environment: +4 to +40 °C 20–95% relative humidity 84–106 kPa (840–1060 mbar) atmospheric pressure

Physical data

Accessories and spare parts

Valve INV-907 including one UniNet cable

(fill port, needle and syringe holder are not included)

| __

__

__

1

| __

__

__

18-1108-40

Injection kit INV-907 including fill port needle and syringe holder| __

__

1

| __

__

18-1110-89

Valve kit INV-907|  |
including channel plate and distribution plate| __

1

| __

18-1109-05

Sample loops 100 μl| __

1

| __

18-1113-98

500 μl| 1| 18-1113-99
1 ml| 1| 18-1114-01
2 ml| 1| 18-1114-02
Superloop, 10 ml| 1| 18-1113-81
Superloop, 50 ml| 1| 18-1113-82
Superloop, 150 ml| 1| 18-1023-85
Cable UniNet, 0.7 m| 1| 18-1109-74
Mounting bracket| 1| 18-1109-11
Teflon tubing, i.d. 1/8”,

o.d. 3/16”

| __

3 m

| __

18-1112-47

Tubing connector for 3/16”|  |
o.d. tubing| 10| 18-1112-49
Ferrule for 3/16” o.d.tubing| 10| 18-1112-48
Stop plug, 5/16”| 5| 18-1112-50
Stop plug, 1/16”| 5| 18-1112-52
Union Luer female/1/16” male| 2| 18-1112-51
Union 1/16” female/M6 male| 6| 18-1112-57
Union M6 female/1/16” male| 8| 18-1112-58
PEEK tubing, i.d. 0.75 mm, o.d. 1/16”| 2 m| 18-1112-53
Teflon tubing, i.d. 0.75 mm, o.d. 1/16”| 2 m| 18-1112-54
PEEK tubing, i.d. 1.0 mm, o.d. 1/16”| 2 m| 18-1115-83
Fingertight connector 1/16”| 10| 18-1112-55

• ÄKTA and UNICORN are trademarks of GE Healthcare companies. GE, imagination at work and GE monogram are trademarks of General Electric Company.
• All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. GE Healthcare reserves the right, subject to any regulatory and contractual approval, if required, to make changes in specifications and features shown herein, or discontinue the product described at any time without notice or obligation.
• Contact your local GE Healthcare representative for the most current information.
• UNICORN Any use of this software is subject to GE Healthcare Standard Software End-User License Agreement for Bio-Sciences Software Products.
• © 2005 General Electric Company – All rights reserved.
• GE Healthcare Bio-Sciences AB, a General Electric company.
• GE Healthcare Bio-Sciences AB
• Björkgatan 30, SE-751 84 Uppsala, Sweden
• GE Healthcare Europe GmbH
• Munzinger Strasse 5, D-79111 Freiburg, Germany
• GE Healthcare UK Ltd
• Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
• GE Healthcare Bio-Sciences Corp
• 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
• GE Healthcare Bio-Sciences KK
• Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan

CONTACT

• www.gehealthcare.com
• GE Healthcare Bio-Sciences AB
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• 751 84 Uppsala
• Sweden
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References

• GE HealthCare | GE HealthCare (United States)

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