ACCU-SCOPE ISC366 Excelis HDMI USB Microscope Camera Instructions

: June 13, 2024
: ACCU SCOPE

Table of Contents

ACCU-SCOPE ISC366 Excelis HDMI USB Microscope Camera
Product Information
Product Usage Instructions
Calibration Instructions
TOOLS
Edit Scale Line
Create Calibration File
Measurement List
CONTACT
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

ACCU-SCOPE ISC366 Excelis HDMI USB Microscope Camera

Product Information

The product is an Excelis HD microscope with CaptaVision software. It is equipped with measurement capabilities and allows for calibration to accurately measure objects.

Product Usage Instructions

Place the stage micrometer on the microscope stage under the CAPTURE tab.
Focus and adjust exposure to obtain the sharpest image.
Click on the Zoom Fit icon located on the far right screen tab. This setting should be used for all measurements.
Toggle the Show Scale Line option to display or hide the scale line on the picture.
Create a calibration file by clicking on the Calibrate option. This allows you to add, edit, and delete calibration files.
Adjust the calibration table decimal precision. The range allowed is from 0 to 7.
View all the measurement data in the Measurement List.
Create multiple layers to apply measurements and save layer information under the Layer option.
Delete all measurements and layers using the Delete All option.
Unlock or lock the measurement operation using the UnLock/Lock option. By default, it is locked.
Select a measurement or change the position of the measurement data using the Select option.
Measure the length of a line using the Line option.
Measure the distance between parallel lines using the Parallel option. You can perform multiple parallel distance measurements by double-clicking to end each measurement.
Measure the length of perpendicular lines using the Perpendicular option. You can perform multiple perpendicular length measurements by double-clicking to end each measurement.
Measure the height, width, area, and perimeter of a rectangle using the Rectangle option.
Draw a circle using the 2-points Circle, 3-points Circle, Diameter Circle, or Concentric Circle options. These options provide the radius, area, and perimeter of the circle. You can perform multiple concentric circle measurements by double-clicking to end each measurement.
Measure the area and perimeter of a polygon using the Polygon option.
Measure the angle of a curve using the Arc option. This option provides the angle, radius, and length of the curve.
Measure angles using the Angle option.
Use the Point option as a counter to count quantities.
Add remarks on the images using the Annotate option.
Delete previous measurements by selecting them and clicking on the Delete option.
Toggle the cross-ruler on or off on the images using the Cross-ruler option. The unit of the ruler depends on the applied calibration file.
Edit the properties of the scale line by double-clicking on it. You can edit its scale character, frame, length, and name.
To measure the real size of samples, you need to create a corresponding calibration file.
Take pictures of the calibration slide with all the required working objectives and resolution. If a reducing lens is used, take pictures with the calibration slide and reducing lens attached.
If only one objective and one resolution are used, one calibration slide picture is enough. The calibration slide picture must be taken with exactly the same lens or microscope settings as the target image taken.
Click on the Create Calibration File option to start creating the calibration file.

Calibration Instructions

How to Calibrate the Microscope for Measurement Using CaptaVision Software

Under CAPTURE tab, place stage micrometer on microscope stage.
Focus and exposure adjust for sharpest image.
On far right screen tab, CLICK “Zoom Fit” icon .

Use this setting for all measurments

TOOLS

Edit Scale Line

Double click on the scale to get its properties and make changes to it.

Edit scale character

Edit the frame of the scale

Edit scale line length and name

Create Calibration File

To measure the samples real size, the corresponding calibration file needs to be created first.

Take pictures of the calibration slide in all the required working objectives and resolution (if a reducing lens is also used in your application, it also requires you to take the calibration slide picture with the reduce lens attached).
- If ONLY ONE objective and ONE resolution is used in the application, one calibration slide picture is enough. The calibration slide picture MUST be taken with exactly the same lens or microscope settings as the target image taken.
Click to start to create calibration file.
Click [Load Image] to load the calibration slide picture taken in Step1.
Click [Distance scaling] and move the cursor to the slide image, draw a line to get the reference length.
- Using longer length as the reference length will give more accurate measurement results. For example, using 10 scale units as reference length will give more accurate result than using 1 scale unit.
Enter the name for the calibration file and the length of the line you draw.
- If you need more than one calibration file, using objective+reducing lens(if it is used)+resolution as the name of the calibration file is recommended. This can help to prevent using the wrong file to do the calibration.
- When keying in the length, please pay more attention to the calibration scale unit and the Measure Unit used here. For example, the calibration scale unit is 0.1mm; the Measure Unit is selected as μm; and the reference length is 10 scale units, so the length should be 10 x 0.1mm x 1000 = 1000 μm.
Click [OK] to confirm the calibration. The new calibration file named “10X” is created in the [Calibrate Table].

Calibration Table

Click [Calibrate Table] to open the calibration table.
Select the correct calibration file for current image measurement.
Using the WRONG calibration file will make the measurement result innacurate. Please make sure the calibration file is correctly corresponding to the current image. Hence, it is useful to name the calibration file with the capturing settings or objective name.