PASCO PS-2600 Wireless Spectrometer (VIS) User Guide

PASCO PS-2600 Wireless Spectrometer (VIS)

Spectrometry Resources

More 21st-century science lab resources are available online! http://pasco.com/spectrometer/

• Software Download
• Video Tutorials & User Guide
• Replacement Parts
• Accessories
• Sample Labs

About PASCO Spectrometry

The PASCO Spectrometry software works with the PASCO wireless Spectrometer as well as Ocean Optics Red Tide Spectrometers*. Version 1.0 is freely available to download for Macs & PCs. The free app for iPads is available in the App Store®.

Application Tools

Tool Icon States
Icons indicate state as follows:

Application Tool Descriptions
Application Toolbar Functions:

Analyze Light

Procedure

1. Attached a Fiber Optics Cable to your Spectrometer.
2. Point the Fiber Optics Cable at the Light Source.
3. Start Data Recording
4. Autoset the Integration Time in the Left-Hand Tools panel
5. Stop Data Recording
6. Select the Source to either Rename or Delete
7. Analyze the Graph with the Options Below

Graph Tools
Analyze Light Graphing Functions:

Light Analysis Tools
Left-Hand Tools Slider can be Revealed or Hidden:

Analyze Solution

Note: You MUST select an Analysis Wavelength with the Coordinates Tool to perform subsequent Concentration and Time based Experiments for the solution you are studying.

Procedure

1. Insert a cuvette containing the blank (solvent)
2. Perform a Dark Calibration
3. Allow the Light source to warm up (~1-2 minutes)
4. Perform a Light Calibration
5. Start Data Recording
6. Insert the cuvette containing the sample
   • Adjust the concentration of the solution if the spectrum is too low or too high
7. Stop Data Recording
8. Select the Solution to either Rename or Delete
9. Analyze the Graph with the Options Below

Details

• Analyze Solution Absorbance / Transmittance Details
  • Select “Absorbance” on the Graph to Switch from Absorbance to Transmittance
• Analyze Solution Fluorescence Details
  • A Calibration is not required for this Measurement
  • After Data Collection is Started, Auto Set the Integration time to obtain the best spectrum.

Solution Types
Solution Analysis types in “Analyze Solution”, “Concentration” and “Time” Experiments.

Graph Tools
Analyze Solutions Graphing Functions:

Solution Analysis Tools
Left-Hand Tools Slider can be Revealed or Hidden:

Analyze Concentration

Procedure

1. Select the Concentration Units in the Table Header to modify (if necessary)
2. Select the Concentration Values in the Table to modify (if necessary)
3. Select the empty Absorbance cell next to the appropriate Concentration value
4. Start Data Recording
5. Press “Check” to Keep the Spectrometer Data
6. Continue for each of the known standard concentration values
7. Stop Data Recording
8. Analyze the Graph with the Options Below

Note: You can always edit the concentration value in a cell. If you suspect the Absorbance value is incorrect, simply select the cell again while the data is being recorded and press “check” with the new Absorbance value.

Unknown Concentration Analysis Details
After multiple known concentration samples have been recorded, an unknown can be analyzed

1. Select the empty Absorbance cell in the Determine Unknown Concentration Table
2. Start Data Collection
3. Press “Check” to Keep the Spectrometer Data
4. Enter a value for the concentration of the Unknown based on the Absorbance Data and a graphical analysis of the known samples.
5. Verify a correct determination of the unknown based on the Graph
6. Reassess if necessary.

Solution Types
Solution Analysis types in “Analyze Solution”, “Concentration” and “Time” Experiments.

Graph Tools
Analyze Concentration Graphing Functions:

Analyze Time

Procedure

1. Start Data Recording
2. Allow the desired amount of time to pass
3. Stop Recording
4. Analyze the Graph with the Options Below.

Analyze Time Details

• Select “Absorbance” on the Graph to Switch from Absorbance to Transmittance or Concentration (Concentration data will only be meaningful if the Absorbance and Concentration relationship is established in a Concentration experiment or by manually adding the slope and intercept.)
• Select the “Calculator” button on the Graph to switch through QiuckCalcs of Y, Y-1 or lnY.

Solution Types
Solution Analysis types in “Analyze Solution”, “Concentration” and “Time” Experiments.

Graph Tools
Analyze Time Graphing Functions:

Time Analysis Tools
Left-Hand Tools Slider can be Revealed or Hidden:

Teacher and Technical Support
For help with Spectrometry and other PASCO products, you can contact PASCO Teacher and Technical Support staff by phone, email, or on the Web.
Phone:
1-800-772-8700 (in the U.S.)
+1 916 786 3800 (worldwide)
Email: [email protected]
Web: www.pasco.com/support/
Tech Notes: www.pasco.com/support/technical- support/technote/

References

• Wireless Spectrometer (Vis) - PS-2600 - Products | PASCO
• Wireless Spectrometer (Vis) - PS-2600 - Products | PASCO
• PASCO scientific | Science Lab Equipment and Teacher Resources
• Support Center | PASCO
• Knowledge Base - Support | PASCO

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