Promega AS1820 Maxwell CSC Whole Blood DNA Kit User Manual
- June 4, 2024
- Promega
Table of Contents
Promega AS1820 Maxwell CSC Whole Blood DNA Kit
The Maxwell® CSC Whole Blood DNA Kit is only available in certain countries. This product meets the essential requirements of EU Directive 98/79/EC on in vitro diagnostic medical devices.
Description
The Maxwell® CSC Whole Blood DNA Kit(a) is used with the Maxwell® Instruments specified in Table 1 to provide an easy method for efficient, automated sample preparation and purification of genomic DNA (gDNA) from human whole blood samples. The Maxwell® CSC Instruments are designed for use with predispensed reagent cartridges and preprogrammed purification procedures, maximizing simplicity and convenience. The Maxwell® method for the CSC Whole Blood DNA Kit can process from one to the maximum sample number of the Maxwell® CSC Instrument in less than 1 hour. The purified DNA can be used directly in a variety of downstream applications such as PCR.
Table 1. Supported Instruments.
Instrument
| ****
Cat.#
| ****
Technical Manual
| Maximum Sample
Number
---|---|---|---
Maxwell® CSC| AS6000| TM457| 16
Maxwell® CSC 48| AS8000| TM623| 48
The Maxwell® CSC Whole Blood DNA Kit purifies nucleic acid from samples using paramagnetic particles, which provide a mobile solid phase to optimize sample capture, washing and purification of gDNA. Maxwell® Instruments are magnetic particle-handling instruments that efficiently bind nucleic acids to the paramagnetic particle in the first well of a prefilled cartridge. The samples are processed through a series of washes before the gDNA is eluted.
Product Components, Storage Conditions and Symbols Key
Maxwell® CSC Whole Blood DNA Kit
For In Vitro Diagnostic Use. Professional use only. Contains sufficient
reagents for 48 automated isolations from 500µl of whole blood samples.
Cartridges are for single use only.
Includes:
- 48 Maxwell® CSC Cartridges (CSCH)
- 50 CSC/RSC Plungers
- 50 Elution Tubes (0.5ml)
- 20ml Elution Buffer
Storage Conditions : Store the Maxwell® CSC Whole Blood DNA Kit at +15°C
to +30°C.
Safety Information: The Maxwell® CSC Cartridges (CSCH) contain ethanol,
isopropanol, guanidine hydrochloride and guanidine thiocyanate. Ethanol and
isopropanol should be considered flammable, harmful and irritants. Guanidine
thiocyanate and guanidine hydrochloride should be considered toxic, harmful
and irritants. Refer to the Safety Data Sheet (SDS) for detailed safety
information.
Maxwell® CSC Cartridges (CSCH) are designed to be used with potentially
infectious substances. Wear appropriate protection (e.g., gloves and safety
glasses) when handling infectious substances. Bleach reacts with guanidine
thiocyanate, which is used in the Maxwell® CSC Cartridges (CSCH), and should
not be added to any sample waste from these cartridges. Adhere to your
institutional guidelines for the handling and disposal of all infectious
substances when used with this system.
Caution: Handle cartridges with care; seal edges may be sharp.
Additional Information : The Maxwell® CSC Whole Blood DNA Kit components
are qualified and quality-control tested to work together. It is not
recommended to mix kit components between different kit lots. Use only the
components provided in the kit. For additional safety information, see the
Safety Data Sheet, available at: www.promega.com.
Symbols Key
Product Intended Use
- The Maxwell® CSC Whole Blood DNA Kit is intended for use, in combination with the Maxwell® CSC Instruments and the Maxwell® CSC Whole Blood DNA purification method, as an in vitro diagnostic (IVD) medical device to perform automated isolation of genomic DNA from human whole blood samples. The purified DNA is suitable for use in amplification-based in vitro diagnostic assays.
- The Maxwell® CSC Whole Blood DNA Kit is intended to be used at temperatures between 15°C and 30°C. Use outside of this temperature range can cause suboptimal results.
- Whole blood samples collected in blood collection tubes containing EDTA, sodium citrate or heparin anticoagulants can be used with the Maxwell® CSC Whole Blood DNA Kit. The table below shows the acceptable time that samples can be stored under different conditions prior to use in the Maxwell® CSC Whole Blood DNA Kit.
Sample Storage
Temperature
| Storage Time
Before Purification
---|---
+15°C to +30°C| Up to 72 hours
+2°C to +10°C| Up to 7 days
–65°C or lower| Indefinitely
- The Maxwell® CSC Whole Blood DNA Kit is not intended for use with samples that have been collected in other types of blood collection tubes or stored outside of the conditions listed below.
- The Maxwell® CSC Whole Blood DNA Kit is intended for professional use only. Diagnostic results obtained using the genomic DNA purified with this system must be interpreted in conjunction with other clinical or laboratory data.
Product Use Limitations
- The Maxwell® CSC Whole Blood DNA Kit is not intended for use with tissue samples or samples from body fluids other than human whole blood.
- The Maxwell® CSC Whole Blood DNA Kit is not intended for use with nonhuman samples, including bacterial and viral samples, or for the purification of RNA.
- The Maxwell® CSC Whole Blood DNA Kit performance has been evaluated by isolating DNA from 500μl whole blood samples with a white blood cell (WBC) count ranging from 4 × 106 to 1.1
- × 107 cells/ml whole blood and eluting the DNA in 60μl. It is not intended for use with samples outside of this range.
- The user is responsible for establishing performance characteristics necessary for downstream diagnostic applications. Appropriate controls must be included in any downstream diagnostic applications using genomic DNA purified using the Maxwell® CSC Whole Blood DNA Kit.
Before You Begin
Materials to Be Supplied by the User
- rotating tube mixer for liquid blood samples
- pipettors and pipette tips for sample transfer into prefilled reagent cartridges
Preparing Whole Blood Samples
The total yield of genomic DNA from whole blood samples depends on the sample
white blood cell count. Each cartridge supplied in the Maxwell® CSC Whole
Blood DNA Kit is designed to purify genomic DNA from 500µl of whole blood with
white blood cells in the range of 4 × 106 to 1.1 × 107 cells/ml whole blood
(values for a normal healthy adult; 1). We recommend performing a white blood
cell count on each sample prior to DNA purification to ensure the sample falls
within this range. Samples outside of this range may not provide optimal
results.
Note: This kit has been tested with human whole blood samples collected
in EDTA, sodium citrate and heparin tubes. Kit performance cannot be
guaranteed with other types of blood collection tubes. Blood samples may be
fresh (stored at +15° to +30°C for up to 72 hours), refrigerated (stored at
+2°C to +10°C for up to 7 days) or frozen (stored at
–65°C or lower) prior to DNA purification. Frozen samples must be thawed
before processing. All blood samples must be thoroughly mixed before use.
Preparing the Maxwell® CSC Whole Blood DNA Cartridge
-
Change gloves before handling cartridges, CSC/RSC Plungers and Elution Tubes (0.5ml). Cartridges are set up in the deck tray(s) outside of the instrument before transferring the deck tray(s) containing the cartridges and samples to the instrument for purification. Place each cartridge in the deck tray(s) with well #1 (the largest well in the cartridge) farthest away from the elution tubes (Figure 2). Press down on the cartridge to snap it into position. Ensure both cartridge ends are fully seated in the deck tray. Carefully peel back the seal so that the entire seal is removed from the top of the cartridge. Ensure that all sealing tape and any residual adhesive are removed from the cartridge.
-
Transfer 500µl of blood sample to well #1 of each cartridge (well #1 is the largest well).
-
Mix the blood sample in well #1 by pipetting 5–10 times to ensure all blood has been transferred. Mixing the sample by pipetting may improve chemistry performance. Change pipette tips between samples.
-
Place one plunger into well #8 of each cartridge.
-
Place an empty elution tube into the elution tube position for each cartridge in the deck tray(s). Add 60µl of Elution Buffer to the bottom of each elution tube.
Notes: -
Use only the elution tubes provided in the Maxwell® CSC Whole Blood DNA Kit. Other elution tubes may be incompatible with the Maxwell® CSC Instruments.
-
Only use the Elution Buffer provided in the Maxwell® CSC Whole Blood DNA Kit. Use of other elution buffers may affect DNA purification performance.
-
Proceed to Section 6, Maxwell® Instrument Setup and Run.
Maxwell® CSC Whole Blood DNA Cartridge Preparation Notes:
Specimen or reagent spills on any part of the deck tray should be cleaned with
a detergent-water solution, followed by a bactericidal spray or wipe and then
water. Do not use bleach on any instrument parts.
Figure 1. Maxwell® CSC Cartridge. Whole blood sample is added to well #1,
and a plunger is added to well #8.
Figure 2. Setup and configuration of the deck tray(s). Elution Buffer is
added to the elution tubes as indicated. Deck tray shown is from the Maxwell®
CSC Instrument ( Cat.# AS6000 ).
Maxwell® Instrument Run
For detailed information, refer to the Technical Manual specific to your Maxwell® CSC Instrument. See Table 1.
-
Turn on the Maxwell® Instrument and Tablet PC. Log in to the Tablet PC, and start the Maxwell® IVD-mode software by double-touching the icon on the desktop. The instrument will proceed through a self-check and home all moving parts.
-
Touch Start on the ‘Home’ screen.
-
Scan or enter the bar code in the upper right corner of the Maxwell® CSC Whole Blood DNA Kit label and press OK to automatically select the method to be run (Figure 3).
Note: The Maxwell® CSC Whole Blood DNA Kit method bar code is required for DNA purification on the Maxwell® CSC Instruments. The kit label contains two bar codes. The method bar code is indicated in Figure 3. If the bar code cannot be scanned, contact Promega Technical Services.
Figure 3.
Kit label indicating the bar code to scan.
The bar code to scan for starting a purification run is shown in the red box,
in the upper right of the kit label. -
On the ‘Cartridge Setup’ screen, touch the cartridge positions to select or deselect any positions to be used for this extraction run. Enter any required sample tracking information, and press the Proceed button to continue.
Note : When using the Maxwell® CSC 48 Instrument, press the Front or Back button to select or deselect cartridge positions for the appropriate deck tray. -
After the door has been opened, confirm that all Extraction Checklist items have been performed. Verify that samples were added to well #1 of the cartridges, cartridges are loaded on the instrument, uncapped elution tubes are present with Elution Buffer and plungers are in well #8. Transfer the deck tray(s) containing the prepared cartridges onto the Maxwell® Instrument platform. Inserting the Maxwell® deck tray(s): Hold the deck tray by the sides to avoid dislodging cartridges from the deck tray. Ensure that the deck tray is placed in the Maxwell® Instrument with the elution tubes closest to the door. Angle the back of the deck tray downward and place into the instrument so that the back of the deck tray is against the back of the instrument platform. Press down on the front of the deck tray to firmly seat the deck tray on the instrument platform. If you have difficulty fitting the deck tray on the platform, check that the deck tray is in the correct orientation. Ensure the deck tray is level on the instrument platform and fully seated.
Note : Check the identifier on 24-position Maxwell® deck trays to determine whether they should be placed in the front or back of the instrument. -
Touch the Start button to begin the extraction run. The platform will retract, and the door will close. Warning: Pinch point hazard.
Note : If using a 48-position Maxwell® Instrument and the Vision System has been enabled, the deck trays will be scanned as the platform retracts. Any errors in deck tray setup (e.g., plungers not in well #8, elution tubes not present and open) will cause the software to return to the ‘Cartridge Setup’ screen and problem positions will be marked with an exclamation point in a red circle. Touch the exclamation point for a description of the error and resolve all error states. Touch the Start button again to repeat deck tray scanning and begin the extraction run. -
The Maxwell® Instrument will immediately begin the purification run. The screen will display the steps being performed and the approximate time remaining in the run.
Notes : -
Touching the Abort button will abandon the run. All samples from an aborted run will be lost.
-
If the run is abandoned before completion, you may be prompted to check whether plungers are still loaded on the plunger bar. If plungers are present on the plunger bar, you should perform Clean Up when requested. If plungers are not present on the plunger bar, you can choose to skip Clean Up when requested. The samples will be lost.
-
When the run is complete, the user interface will display a message that the method has ended.
End of Run -
Follow on-screen instructions at the end of the method to open the door. Verify that plungers are located in well #8 of the cartridge at the end of the run. If plungers have not been removed from the plunger bar, follow the instructions in the Technical Manual appropriate to your Maxwell® Instrument (see Table 1) to perform a Clean Up process to attempt to unload the plungers.
-
Remove the deck tray(s) from the instrument immediately following the run to prevent evaporation of the eluates. Remove elution tubes containing DNA, and cap the tubes.
Note : Following the automated purification procedure, the deck tray(s) will be warm. To remove a deck tray from the instrument platform, hold onto the deck tray by its sides.
Ensure samples are removed from the instrument before running a UV sanitation protocol to avoid damage to the nucleic acid. -
Remove the cartridges and plungers from the Maxwell® deck tray(s). Discard as hazardous waste according to your institution’s procedures. Do not reuse Maxwell® CSC Cartridges, CSC/RSC Plungers or Elution Tubes.
Troubleshooting
For questions not addressed here, please contact your local Promega Branch Office or Distributor. Contact information is available at: www.promega.com. E-mail: techserv@promega.com
Reference
Henry, J.B. (2001) Clinical Diagnosis and Management by Laboratory Methods,
20th ed., W.B. Saunders Company, 509.
Related Products
Instrument and Accessories
Product
| ****
Size
| ****
Cat.#
---|---|---
Maxwell® CSC 48 Instrument| 1 each| AS8000
Maxwell® CSC Instrument| 1 each| AS6000
Maxwell® RSC/CSC Deck Tray| 1 each| SP6019
Maxwell® RSC/CSC 48 Front Deck Tray| 1 each| AS8401
Maxwell® RSC/CSC 48 Back Deck Tray| 1 each| AS8402
Elution Tubes (0.5ml)| 50/pack| AS6201
Elution Magnet, 16 Position| 1 each| AS4017
Elution Magnet, 24 Position| 1 each| AS4018
*For In Vitro Diagnostic Use. This product is only available in certain countries.| |
Maxwell ® **CSC Reagent Kits**
Visit www.promega.comfor a list of available Maxwell® CSC purification kits.
| |
Maxwell® CSC Reagent Kits
Visit www.promega.com for a list of available Maxwell® CSC purification kits.
© 2021 Promega Corporation. All Rights Reserved. Maxwell is a registered trademark of Promega Corporation. Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information. All prices and specifications are subject to change without prior notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA800-356-9526 · 608-274-4330 · Fax608-277-2516 11 www.promega.com TM660 · 8/21
Documents / Resources
|
Promega AS1820 Maxwell CSC Whole Blood DNA
Kit
[pdf] User Manual
AS1820 Maxwell CSC Whole Blood DNA Kit, AS1820, Maxwell CSC Whole Blood DNA
Kit, Whole Blood DNA Kit, Blood DNA Kit, DNA Kit
---|---
References
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