SS-L02 FMCG Listeria Kit Instructions
- June 12, 2024
- FMCG
Table of Contents
TSC SS-L02 FMCG Listeria Kit
All samples should be kept cool until testing is performed. Please visit the website for Precautions/Limitations.
STEP 1
Identify the site to be sampled. It is recommended to use a template for accurate results where possible (TS/15-T).
STEP 2
Hold the swab with both hands, Pull down and twist the swab cap away from the tube; breaking the security seal in the process.
STEP 3
Remove the swab from the tube using the cap as a holder. Do not touch the swab tip or shaft and make sure that it does not touch any surface before sampling. Do not discard the tube.
STEP 4
Sample designated area using a template where possible. If the area permits, swab in 3 planes (horizontal, vertical, and diagonal) whilst rotating the swab between the forefinger and thumb.
STEP 5
When sampling is completed insert the swab back into the tube and push the cap
on tightly.
STEP 6 (DELAYED TEST)
Write sample details on the tube label. Store at 4-8°C (or cool & dry as possible) and send it to the laboratory within 24 hours to initiate the chromogenic test.
STEP 7 (IMMEDIATE TEST)
Alternatively (where permitted), Immediately insert the swab into the desired
test media tube until the breakpoint is level with the top of the vial. Pull
down on the swab shaft whilst holding the tube straight to activate the
breakpoint. Replace the Cap & agitate lightly.
STEP 8 (CHROMOGENIC TEST)
Place tubes into a rack & incubate them vertically at 25+1°C for 24-48 hours.
(Other temperatures may be used, please See the webpage for details)
STEP 9 (READING RESULTS)
Read results after 18-24 hours of incubation. If the test is negative,
continue incubation up to 48 hours before the final reading. The change of
media color from yellow to black indicates the presumptive presence of
Listeria species.
STEP 10 (CONFIRMATION when required)
Confirm using the positive (black) liquid in a validated rapid test or sub-
culture 1-5ul of liquid onto a selective agar plate such as Oxford/Palcam or
ALOA & streak to single colonies. After approx. 18-24 hours to examine for
typical Listeria colonies.
Alternatively, use
- PCR Methods (after isolation)
- ElA or Rapid Test (after isolation) For suspected Listeria positive tests perform a Catalase Test. Please see the info sheet & product webpage links for details
Have you considered it?
Sterile Sampling Sponge – TS/15-B
Technical Service
Consultants Ltd
www.tscswabs.co.uk
Tel: +44 (0)1706 620600