FMCG INDUSTRY SOLUTIONS SS-L03 Swabsure Salmonella Kit Instruction Manual

GUIDANCE FOR USE
SwabSURE Salmonella – SS – L03

SS-L03 Swabsure Salmonella Kit

All samples should be kept cool until testing is performed. Please visit the website for Precautions/Limitations.
STEP 1
Identify the site to be sampled. It is recommended to use a template for accurate results where possible (TS/15-T).

STEP 2
Hold the swab with both hands, pull down and twist the swab cap away from the tube; breaking the security seal in the process.

STEP 3
Remove swab from tube using the cap as a holder. Do not touch the swab tip or shaft and make sure that it does not touch any surface before sampling. Do not discard the tube.

STEP 4
Sample designated area using a template where possible. If the area permits, swab in 3 planes (horizontal, vertical and diagonal) whilst rotating swab between forefinger and thumb.

STEP 5
When sampling is completed insert swab back into the tube and push cap on tightly.

STEP 6 (DELAYED TEST)
Write sample details on tube label. Store at 4-8°C (or cool & dry as possible) and send to the laboratory within 24 hours to initiate the colour test.

STEP 7 (IMMEDIATE TEST) Alternatively (where permitted), immediately insert swab into desired test media tube until the breakpoint is level with the top of the vial. Pull down on the swab shaft whilst holding the tube straight to activate the breakpoint. Replace Cap & agitate lightly.

STEP 8 (COLOUR TEST)
Place tubes into a suitable rack & incubate them vertically at 36±1°C.

STEP 9 (READING RESULTS)
Read results after 18-24 hours. If the test is negative, the test can be reported as negative. Change of media colour from pale purple to black indicates presumptive presence of Salmonella species.

STEP 10 (CONFIRMATION when required)
Confirm using the positive (black) liquid in a validated rapid test or subculture onto an appropriate selective agar such as XLD or a chromogenic Salmonella agar. After approx. 18-24 hours examine for typical colonies. Alternatively use:

1. PCR Methods
2. EIA or Rapid Tests (after isolation)
   Please see webpage & info sheet for further details.

Have you considered? Sterile Sampling Sponge – TS/15-B

www.tscswabs.co.uk
Tel: +44 (0)1706 620600
FMCG Industry Solutions Pty Ltd
ABN 22 135 446 007
Factory 11A, 1 – 3 Endeavour Rd,
Caringbah NSW 2229
**[www.fmcgis.com.au](http://www.fmcgis.com.au)
**sales@fmcgis.com.au
1300 628 104 or 02 9540 2288

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