DLAB DM0506 Clinical Centrifuge User Manual

DM0506 Clinical Centrifuge
User Manual

This user manual needs to be read before operating this product.
Please follow the safety instructions provided in this manual.

Safety Warnings

The symbol is an internationally accepted safety mark. Please carefully read and fully understand the following safety rules:

• Comply with the operation requirements contained herein and ensure safe operation.

• Carefully read all safety information and reminders provided in this manual.

• Safety information is marked as follows. The safety symbol is combined with the “Warning” and “Caution” respectively, reminding users of the potential danger. These two combinations and the “reminder” symbols are defined as follows:
  Warning: personal danger.
  Warning against potential dangers, which might result in injury or death if the requirements contained herein are not strictly complied.
  Caution: Potential danger to instrument
  Ensure to comply with all the safety requirements as mentioned to avoid potential dangers of damage to the instrument
  Reminder: matters that generally call for attention.

• Do not use this centrifuge in any manner not mentioned in this user manual.

• In case of any problem, please contact the vendor/supplier.

• This user manual provides complete details of the potential dangers, however users are advised to stay alert against unpredictable circumstances and use this centrifuge with care.

Warning:

• This centrifuge is non-explosion proof and may not be used for separation of flammable or explosive samples.
• Do not install this centrifuge in the vicinity of any flammable gas or chemical substances.
• Do not place anything that causes danger within 30cm radius of this centrifuge.
• Take appropriate safety measures before centrifugation of any toxic, radioactive or pathogenic organisms. If the microorganism sample being centrifuged is secondary hazard (as defined in the WHO’s “Laboratory Biosafety Manual”), ensure to use biological sealing devices.
• Ensure to sterilize it according to decontamination procedures that are mentioned under sterilization section.
• If there is any need for onsite assistance, sterilize and decontaminate the centrifuge in advance and notify the service representative about the details.
• Never touch the power cable/switch with wet hands and avoid electric shocks.
• As a safety measure, ensure to maintain at least 30cm distance from the centrifuge while in operation.
• For safety reasons, when the centrifuge is running, the personnel should maintain a 30cm distance from the centrifuge.
• Never open the lid while the rotor is in operation.
• The centrifuge should be opened for repair/ dismantling by trained staff only.

**Caution**

• Ensure that the centrifuge is stably placed on level and solid work bench before any operation.

• Ensure that the angle between the outer lid and housing is larger than 70° while opening the lid.

• Never place hands/any other things in between the outer lid and the housing.

• Don’t move or lean against the centrifuge if it is in operation mode.

• If there is any liquid found in the centrifuge, ensure to wipe it OFF with a cloth in time to prevent sample contamination.

• Ensure that the centrifuge chamber is left clean and free of any foreign objects/ tube fragments before each and every operation

• Reminders about rotor:

• Check and ensure that the rotor surface is free of corrosions/ damages before its operation.
  (1) The set rotation speed of the centrifuge should not exceed the allowed minimum speed of the rotor assembly and accessories (rotor and adapter) and ensure to run the centrifuge under minimum allowed speed.
  (2) Don’t exceed the allowed amount of imbalance.
  (3) The centrifuge tubes used should be within their allowed capacity.
  (4) If the rotor has a lid, ensure to tighten it before operation.
  (5) Use genuine accessories only.

• If there is any abnormality or strange noise observed in operation, please stop the centrifuge operation and contact the service center and intimate the failure code immediately.

• Earthquake might cause damage to the centrifuge. If any abnormality occurs, please contact the service center.

Performance indicators

deceleration; predefined and customizable programs.

Conformity to standards

The centrifuge’s structure conforms to the following safety standards:
EN 61010-1
EN 61010-2-10
UL 3101-1
The centrifuge’s structure conforms to the following electromagnetic compatibility standard:
EN 61326-1
Compliant with the following EU standards:
EMC standard: 89/336/EWG
Mechanical Design: 73/023/EWG

Environmental requirements

3.1. Basic operating requirements

1. Power supply: single phase, 100-240V, 50/60Hz, 2A, sinusoidal waveform.
2. Ambient temperature: 2~40℃.
3. Relative humidity: ≤80%.RH
4. No vibration or air flow present nearby that might affect performance.
5. No conductive dust, explosive gas or corrosive gas exists in ambient air.

3.2. Transport and storage conditions

1. Range of ambient temperature: -40℃-55℃.
2. Range of relative humidity: ≤93%.RH
3. The centrifuge must remain upright while in transit, suitably protected using wooden Kart box
4. Lift the centrifuge by the chassis only.
5. Pay attention to the centrifuge’s weight while in transit (see “1.Performance indicators”).

Installation

Users must strictly comply with the installation instructions contained in this chapter.
Be advised! Remove the rotator before moving the centrifuge.
Warning

• Improper power connection might damage the centrifuge.
• Before connecting the power supply, please check the power supply for compliance with the requirements.

4.1. Mounting position

1. This centrifuge must be mounted on a solid, flat and tabletop with contact between the four feet of the centrifuge and the tabletop. Don’t mount the centrifuge on any sliding tabletop, otherwise significant vibration might occur. Carefully place the centrifuge to avoid damage.
2. The ideal ambient temperature is 20°C ±5°C and the ambient temperature should not be more than 30℃. Avoid direct sunlight on this centrifuge.
3. Place the centrifuge properly and keep >30cm space from the rear and >10cm space on both sides to ensure effective air cooling.
4. Ensure that there is no water leakage/ heat loss near the centrifuge as it may cause the rise in temperature and thereby leading to centrifuge failure.

4.2. Connection between power cable and ground wire
Warning

• Don’t touch the power cable with wet hands and avoid electric shocks.
• Ensure that the centrifuge is well grounded.

1. This centrifuge uses two-core power cable and two-core flat plug, the latter of which may be directly connected to the power socket.
2. Ensure the rated current of the power socket is correct (>10A) and it complies to local electrical safety regulations before connecting the device to power supply.

Structure

Operating panel

release the door lock. When the rotor is still running, the door will remain locked.
2| | Program #1 key| Press this key load/edit the predefined program #1.
3| | Parameter knob| Rotate this knob to adjust the parameter setting of speed, RCF, timer or deceleration.
4| | Program #2 key| Press this key load/edit the predefined program #2.
5| | Start/Stop key| When the centrifuge is ready to run, press this key to start running; While the centrifuge is running, press this key to stop.

The main display is shown in Figure 6.2. At this time, the speed is set at 5,000rpm, indicating the set running time of 10 min.
When the speed icon rotates, it indicates that the machine is in run mode. The icon rotates quicker at higher centrifuge speed.
The time display icon divides the entire runnig time into 10 equal parts, displaying the ratio of elapsed time to the total time.

Rotor preparation

7.1. Prepare the samples to be separated
7.2. Place samples into the centrifuge tube

The amount of sample should not exceed the working volume capacity of the centrifuge tube.
Caution: Adding excessive samples into the centrifuge tube will result in leakage, therefore don’t add excessive samples.

7.3. Ensure the balanced centrifuge tube

• Although this centrifuge may be used with visually confirmed balance, it is suggested that samples be weighed using a balance to ensure balanced centrifuge tube in order to prolong the service life of centrifuge.
• Although the imbalance to some extent is allowed (See 11.1.2 Rotor and adaptor), don’t run this centrifuge under poor balance conditions.

7.4. Check the rotor
Check the rotor for any corrosion or scratch before use.
Caution:

• Avoid using the rotor with scratches or corrosion.
• Never use the rotor of other brands/ specifications on this centrifuge.
• Do not expose the rotor and its accessories to direct sunlight/Ultraviolet.

7.5. Insert the centrifuge tubes symmetrically onto the rotor in place without imbalance
Caution:

• Ensure to tighten the rotor to the main shaft firmly and the lid is secured properly on the rotor. Otherwise, the rotor might fall OFF while the centrifuge is in operation, resulting in centrifuge or rotor damage.
• Tighten the rotor lid and rotor firmly.

Operation

8.1. Normal operation
Turn ON the power switch, the most recent settings will be loaded and shown on the LCD display as in Fig. 8-1.

Figure 8.1 The most recent settings loaded and shown

• The speed set to 5000rpm and the timer set to 10 min.
• The lid lock is released.

8.1.1. Rotor installation and replacement

Figure 8.2 Rotor installation

• When placing the rotor, ensure the full contact between the rotor and the motor shaft.
• After placing the rotor in place, rotate the rotor gently with hands to check for normal operation of the rotor. Check and adjust the rotor position once again.
• Hold the rotor with one hand, and rotate the locking nut clockwise using the rotor wrench with the other hand. Tighten the rotor and main shaft firmly.
• Close the lid and run the centrifuge.
• Dismantle the rotor in a similar manner with opposite rotating direction (counterclockwise).

Caution

• Ensure the rotor is properly mounted on the centrifuge firmly.

8.1.2. Set operating parameters

The parameter knob is used to adjust the parameter settings. Press the knob gently to select the setting, make it blinking on the display and ready to be adjusted. Rotate the knob clockwise to increase and counterclockwise to decrease the parameter. Rotate the knob quicker to adjust the parameter more dramatically. The minimum rotation speed increment is 10rpm, the minimum RCF increment is 10g and the minimum timer increment is 1 second.

1. Set the rotation speed
   ◼ Press the parameter knob to choose the rotation speed parameter value in rpm. The speed parameter should blink on the display.
   ◼ Rotate the know to adjust the rotation speed value to desired setting.
   ◼ The minimum speed is 300rpm, and the minimum increment is 10rpm.
   ◼ The parameter increases or decreases in a cyclic way. Turn the knob clockwise or anticlockwise to increase or decrease the adjusting parameter values. If you continue rotating the knob when the parameter hits the upper limt, it will start from the lower  limit again.

2. Set the run time
   ◼ Press the parameter key to select the time parameter and wait until its value blinks.
   ◼ Rotate the knob and set the timer to desired setting within the range of 30s-99min.
   ◼ Set the timer to HD to disable it and run the centrifuge in a mode without time limit.

3. Modify the predefined program
   ◼ Press the program #1 key (or program #2 key ) to load the settings of this predefined program. Press it again and hold it for 5 seconds to edit the settings. Press it again and again to cycle between speed, RCF and timer setting. Rotate the knob to adjust the parameter of current setting. Wait for 7 seconds and the settings will be updated for the corresponding predefined program.

8.1.3. Start Run

1. Press the key to start running.
   ◼ The rotor will start rotating only when the lid is locked.
   ◼ Timer starts to count down as soon as the speed reaches the set value.

2. Inquire and change the settings
   ◼ The setting can be modified when the centrifuge is running at the set speed.
   ◼ Press the knob to go back to settings screen. Follow the instructions in 8.1.2 to review and modify the settings. Wait for 7 seconds, the settings will be updated, the display will go to the running screen again and the centrifuge will run on the new settings.
   ◼ The timer will continue to count down without reset.

3. Error message
   ◼ The centrifuge will automatically stop if any failure occurs while running, with the error code shown in the timer section on the LCD display. Please look up the cause of failure and take appropriate action in Table 10.1.

8.1.4. Stop running

1. When the time is up or you press the Start/Stop key while running, the centrifuge will stop running.
   ◼ The centrifuge will beep to notify the user when the rotor fully stops.

2. The lid lock will be released when the rotor fully stops.
   ◼ Press the key to release the lock if the lid is still locked.
   ◼ The current settings will be stored. It will be loaded automatically as soon as the centrifuge is switched ON next time.

3. Retrieve the rotor and samples.

8.2. RCF operation
Turn power switch ON and set the centrifuge to RCF (relative centrifugal force) mode.
Caution

• The relative centrifugal force set should not exceed the maximum relative centrifugal force allowed by the centrifuge tube and its adaptor.
• The relative centrifugal acceleration is calculated based on the maximum centrifugal radius and operating speed of the rotor. (See 12 RCF calculation).
• Press the parameter knob to choose the rotation speed unit as xg. If the RCF value flashes it indicates that the RCF value can be set as it is in adjustment mode.
• Turn the knob to adjust the relative centrifugal acceleration, in increments of 10xg.
• Wait for 7 seconds to update the settings.
  See Section 8.1 for operation instructions.

8.3. Set deceleration level
Press and hold the knob for 5 seconds, after a beep sound, you can adjust the deceleration between 2 levels by rotating the knob. Level 1 is the default, and it is suitable for most applications. Set it to Level 2 for slower deceleration.

Maintenance and servicing

9.1. Cleaning

Caution

• Possible damage to the centrifuge if you clean or sterilize it not following the instruction in this manual.
• Disconnect the power supply before cleaning the centrifuge.

1. Centrifuge
   ◼ The color of housing might change and the label thereon might fall OFF if the centrifuge is exposed to ultraviolet for a prolonged period of time and hence cover the centrifuge with cloth to avoid exposure to light.
   ◼ Clean the centrifuge using a cloth/ sponge soaked with neutral cleansing agent in case if it is dirty after use.
   ◼ The centrifuge can be sterilized using cloth soaked with 70% alcohol solvent.

2. Centrifugal chamber
   Caution
   • Never pour water or other solvents directly into the centrifugal chamber as they might enter the drive unit and cause corrosion or damage to the bearings.

3. Drive shaft
   ◼ It is suggested that the drive shaft be subjected to periodical maintenance by wiping it using soft cloth and applying a thin layer of silicone grease on it.

4. Outer Lid
   ◼ Clean or sterilize the outer lid in the same manner as mentioned under subsection (1) centrifuge.

5. Rotor
   ◼ If the rotor is left unused for a prolonged period of time, remove the rotor and its lid (if there is any rotor lid) from the centrifugal chamber, and place the rotor upside down to dry the rotor hole and prevent corrosion.
   ◼ Use clean water to rinse the rotor cavities if there is sample leakage from the centrifuge tubes. Dry the rotor thoroughly and apply a thin layer of silicone grease on it.
   ◼ Clean the rotor regularly (every 3 months recommended). Ensure the cavities and the drive shaft hole are clean. Apply a thin layer of silicone grease.

Common failures and solutions

10.1. List of common failures
This centrifuge is capable of onboard diagnosis. When the centrifuge fails, the time display window will indicate the failure code, leading to the immediate identification of possible failure causes.

the failure and reconnect the power supply.
Abnormal vibration| • Misfitting between the rotor and the drive shaft.
• The tubes are not balanced.| • Mount the rotor properly again.
• Balance the tubes with scale and load them again.
Alarm code indicated on the time display| E-02
Lid failure| • The lid is opened while running.
• is pressed when the lid is opened.| • Immediately close the cover.
Close the outer lid before operation.
E-09
Imbalanced rotor| • The tubes are not balanced.| • Balance the tubes with scale and load them again.
Others| Check the service manual.| Contact the service center.

Table 10.1 Common failures and solutions

◼ Error code E-01～E-09 are related to erroneous operation. The centrifuge may continue running after elimination of the failure.

10.2. How to open the outer lid
10.2.1. When turned ON
Reminder: When the centrifuge is switch power ON, open the outer lid only when the rotor is not running.

1. When the centrifuge is turned ON, the outer lid opens automatically.
2. At the end of centrifuge operation, the outer lid remains locked.
3. When the rotor stops, press key and unlock the outer lid and one can observe that the lid can be opened now.

10.2.2. When power is OFF
When the lid cannot be opened in case of unexpected power failure, the outer lid may be opened as follows:

1. Check whether the rotor is in run mode.
   ◼ Look at the rotor through the transparent lid and ensure that it is fully stopped.

2. Release the lock manually by the lever.
   ◼ The lever is hidden under the right bottom of the centrifuge.
   ◼ Pull the lever to the right and release the lock. Then open the lid.

Introduction to rotor and centrifuge tube

Caution

• Carefully read the user manual and correctly install and use the rotor correctly.
• Don‘t exceed the maximum allowed speed of the rotor, test tube and adaptor. The maximum allowed speed by certain adaptors is lower than the maximum speed of the rotor and check before operation.

11.1.Rotor description
11.1.1. Rotor structure Figure 11.1 Rotor structure

11.1.2. Rotor and adaptor
The rotors and adaptors which are suitable for use with this centrifuge are listed as follows:

Rotor Model| Rotor No.| Centrifuge tube| Adaptor| Max speed (peed)| Max RCF (xg)| Balance Tolerance(*)
---|---|---|---|---|---|---
Mass| Volume(**)
A6-ISP| 1| 1.5/2.0m1 micro centrifuge tube| A2P17| 5000| 1817| 2.0g/tube|
(p13x75 Blood collection tube| A5P17| 5000| 2236| 2.0W tube|
15ml tube| | 5000| 2600| 2.0W tube|
(p13x100 mm tube| | 5000| 2600| 2.0W tube|
φ16x100mm tube| | 5000| 2600| 2.0g/ tube|

Table 11.1 List of rotors and adaptors
* : The tolerance is applied to tube pairs placed symmetrically in the rotor.
**: The volume tolerance is a rough estimate. There is no strict match to mass tolerance.
11.1.3. Precautions

1. The density of sample that the centrifuge rotor can separate is less than 2.0g/ml. If the density of sample to be separated exceeds 2.0g/ml, please calculate the allowed rotation speed using the following formula: Allowed rotation speed (rpm) = maximum rotation speed × (2.0 (g/ml) /sample density (g/ml))½
2. If the rotor is left unused for long time, please remove the rotor from the centrifugal chamber, remove the rotor lid and place the rotor upside down to dry the rotor hole and prevent corrosion.
3. If any sample leaks into the rotor hole, flush the rotor hole with clean water and apply a thin layer of silicone grease on the rotor surface after it dries up.
4. It is suggested that the rotor to be cleaned once in every three months to ensure cleanliness of the tube hole and main shaft hole before applying a thin layer of silicone grease.

11.1.4. Autoclaving
Warning

• The high strength plastic rotor CANNOT be autoclaved.

11.2. Centrifuge tube
11.2.1. Please clean and sterilize the centrifuge tube by reference to the following table.

[Table 11.2 Conditions for cleaning and sterilization of centrifuge tube| 0: Yes| X: No
---|---|---
Condition Material| PA| PC| PP
Cleaning| Fluid cleaning| Acidic cleaning agent (pH5 or lower)| X| X| X
Acidic cleaning agent (above pH5)| 0| 0| 0
Alkaline cleaning agent (above pH9)| 0| X| 0
Alkaline cleaning agent (pH9 or lower)| 0| 0| 0
Neutral cleaning agent (pH7)| 0| 0| 0
70°C hot water| 0| 0| 0
Ultrasonic cleaning| Neutral cleaning agent (pH7)| 0| 0| 0
Sterilization| Autoclaving| 115°C (0.7kg/cm²) 30min| 0| 0| 0
121°C (1.0kg/cm²) 20min| X| 0| 0
126°C (1.4kg/cm²) 15min| X| X| X
Boiling sterilization| 15-30min| 0| 0| 0
Ultraviolet sterilization| 200-300nm| X| X| X
Gas sterilization| Ethylene oxide| 0| X| 0
Formaldehyde| 0| 0| 0

PA: polyallomer PC: polycarbonate PP: polypropylene
11.2.2. PC centrifuge tube cleaning
PC material has relatively low chemical stability to alkaline solvent, therefore use of cleaning agent with pH value of over 9 should be avoided. Some neutral cleaning agents still have pH value of over 9 after being diluted as recommended by the vendor, therefore use of cleaning agent with pH value of 7-9 only is recommended.
11.2.3. Autoclaving of PA, PC and PP centrifuge tube
PA begins softening at the temperature of 120℃, while PC and PP begin softening at 130℃.
Generally, PA may be sterilized for 30 min at 115℃（0.7 kg/cm²), while PC and PP may be sterilized for 20 min at 121℃（1.0 kg/cm²). Too high temperature would result in deformation of centrifuge tube.
When autoclave is used, take the following steps:

1. Place the centrifuge tube upright with opening facing upward. If the centrifuge tube is placed in an inclined or horizontal manner, it will deform due to the effect of gravity.
2. Remove the threaded cover and inner cover to prevent deformation or crack of the centrifuge tube.
3. Take the centrifuge tube only when the autoclave cools down to the room temperature.

11.2.4. Service life of centrifuge tube
The service life of plastic centrifuge tube depends upon the nature of sample, rotor speed and centrifugation temperature. When the plastic centrifuge tube is used for centrifugation of conventional neutral samples (pH5-pH9), its estimated service life at the maximum rotation speed is as follows:
High-quality centrifuge tube (PA, PC, PP): 30-50 times.
Conventional centrifuge tube: about 10 times (frequency of use may be increased in case of low-speed application use)
The service life of centrifuge tube is also related to the cleaning and sterilization conditions.
Note: Never use any centrifuge tube with cracks.

RCF calculation

Relative centrifugal force (RCF）can be calculated using the following formula:
RCF=1.118×r×n²×10-⁵
r-rotation radius, unit: cm; n-rotation speed, unit- rpm

Warranty

13.1. Unit warranty
The entire unit will have one-year warranty period commencing from delivery date under the conditions of normal maintenance.
13.2. Rotor warranty
The rotor will have 5-year warranty period from the date of delivery. Don’t use any rotor damaged due to corrosion or fatigue. The damage to the entire unit or rotor due to any of the following reasons is outside the scope of warranty:

1. Damage due to improper installation;
2. Damage due to brutal or improper operation;
3. Damage due to relocation or transport after completion of installation;
4. Damage due to dismantling or modification by any unauthorized entity or individual;
5. Damage due to use any parts not supplied by our company, such as rotor and adaptor;
6. Damage due to natural disasters, including fire and earthquake;
7. Wearing parts and parts with warranty period.

After-sales services

To ensure safe and efficient operation of the centrifuge, periodical maintenance is required. If the centrifuge fails, don’t attempt to repair it. Please contact the service center.

DLAB Scientific Co., Ltd.

Documents / Resources

| DLAB DM0506 Clinical Centrifuge [pdf] User Manual
DM0506, DM0506 Clinical Centrifuge, Clinical Centrifuge, Centrifuge
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