DLAB Scientific Co Ltd SP-UV10 UV Visible Spectrophotometer User Manual

UV Visible Spectrophotometer
User Manual

SP-UV1000 SCI-UV1000
SP-V1000 SCI-V1000
SP-V1100 SCI-V1100
SP-UV1100 SCI-UV1100
SP-UV3101 SCI-3101UV
SP-UV2102 SCI-2102UV
SP-UV3102 SCI-3102UV
SP-UV2101 SCI-2101UV
SP-XUV5102 SCI-5102XUV
SP-XUV5101 SCI-5101XUV

Introduction

I. Safety
The instrument design complies with the safety specifications set forth by the National Standard of the People’s Republic of China GB4793.1-2007/IEC610101:2001 (i.e., Safety Requirements for Measuring, Control and Lab Electrical Equipment: Part 1) and the National Standard of the People’s Republic of China GB9706.1-2007/IEC60601-1:21988) (i.e., Medical Electrical Equipment: Part 1).

II. About the device
SP-UV1000/SP-V1000 serial model visible spectrophotometer is an electronic laboratory testing equipment.
□ Instrument use mode: Intermittent use
□ Presence or absence of overvoltage (current): No
□ Class of pollution: 2

III. Description of related symbols

| General Warning, danger
---|---
| Warning, electricity hazard
| Warning, hot surface
| Mandatory, Connect an earth terminal to the ground
| Fuse

Crossed out recycle bin, this device is to be recycled by the designated electronic equipment disposal department or vendor after use.

Overview

SP-UV1000/SP-V1000 visible spectrophotometer is characterized by wide wavelength range, high sensitivity, powerful functions, ease of use, simple structure and acceptable appearance, especially the application of large- screen LCD, highprecision A/D converter and nonvolatile memory, which makes the instrument uniquely advantageous among the products of the same grade and widely applicable chemical, pharmaceutical, biochemical, metallurgical, light industry, textile, materials, environmental protection, medical assay and education industries, as one of the important quality control instruments in the analytical testing industries and an essential instrument in conventional labs.

I. Instrument rationale and structure
The rationale of spectrophotometric analysis involves using the selective absorption of light of different wavelengths by samples to perform qualitative and quantitative analysis of the samples and judging the structure and chemical composition of substances through analysis of absorption spectrum.
This instrument works on the principle of relative measurement, i.e., selecting a certain solvent (distilled water, air or test sample) as the reference solution and setting its transmittance (i.e., transmission rate T) as 100%, while the transmittance of the testing sample is derived with reference solution as reference standard. The changes in transmittance (transmission rate T) will have a certain functional relation with the concentration of the test sample and it complies with the Lambert-Beer’s Law within a certain range.
T=I/Io
A=KCL=-log I/Io
Wherein T transmittance (transmission rate)
C solution concentration
K absorption coefficient of solution
L length of liquid layer in light path
I intensity of light irradiated on the photoelectric converter through tested sample
Io intensity of light irradiated on the photoelectric converter through reference test sample.
SP-UV1000/SP-V1000 ultraviolet/visible spectrophotometer is a domestically leading new-generation UV-VIS spectrophotometer. These are developed based on this rationale, in conjunction with the modern precision optics, the latest microelectronics and other high-end new technologies.

II. Key technical indicators
SP-UV1000/SP-V1000 serial model key technical indicators are as follows:

III. Key functions
SP-UV1000/SP-V1000 serial model visible spectrophotometer has three major functions.

• Photometric measurement
  **** The measurement modes are switchable (absorbance, transmission rate and energy) and the measurement results (up to 200 groups) can be saved in the instrument’s memory and printed.

• Quantitative measurement
  Standard curve can be established using either coefficient method or standard sample calibration method, and the regression equation (up to 200 groups) and measurement results (up to 200 groups) can be saved in the instrument’s memory or printed.

• System application
  Turn ON and OFF deuterium and tungsten lamps, calibrate dark current, calibrate wavelength, export data and select measurement mode and restore factory settings.

Instrument Installation

I. Environmental requirements
SP-UV1000/SP-V1000 serial model visible spectrophotometer is designed in response to the effects of environmental factors on the instrument. To ensure normal operation of the instrument and achieve an extended service life, check installation environment condition before installation.
The environmental requirements that ensure normal operation of the instrument are as follows:

• Avoid high-temperature and high-humidity environments
  The instrument shall be installed away from high-temperature and high-humidity environments. The instrument shall be used at 16~35℃ and 45-80% RH.

• Keep the instrument away from outside magnetic field interferences
  Ensure to place the instrument away from magnetic/ electric field and highfrequency waves emittingf electronic devices as much as possible.

• Keep the instrument away from corrosive gases
  Please don’t install the instrument in the presence of corrosive gases such as chlorine, hydrochloric acid gas, hydrogen sulfide gas and sulfurous acid gas in air significantly exceeds the applicable standards.

• Instrument must be placed on a steady surface
  Place the instrument on a steady and vibration free surface. The instrument’s fan should be sufficient to allow it vent smoothly.

• Supply voltage
  The power supply for normal operation of the instrument is AC 220±22V@ 50±1Hz or 110±11V@60±1 Hz.

• Power supply must be well grounded
  The instrument should preferably use a standalone power socket and the power supply should be well grounded in order to prevent abnormal operation of the instrument.

• Regulated power supply
  When the local voltage is unstable, please equip the instrument with regulated power supply.

• Instrument should be protected from direct sunlight.

• Maintain dust-free environment.

II. Check supply voltage
Supply voltage varies greatly from one country to another. The input power supply to any devices sold by DLAB in mainland China is 220V/50Hz.
Ensure to check the supply voltage before installation, in order to avoid damages to the instrument.

III. Installation
The installation of SP-UV1000/SP-V1000 serial model visible spectrophotometer is very simple. Please follow the following steps:

Step 1. Check the contents of package
Open the package and carefully and examine its contents against the packing list. Please contact us or our dealer immediately if any item inside the package is found missing or damaged.

Step 2. Place the instrument
Take out the instrument and gently place it on a steady surface.

Step 3. Install the printer (an optional accessory)
Check and ensure that the power switch on the printer is OFF and connect the data cable of the printer in the printer port of the instrument.

Step 4. Install the power cable
Ensure to switch power OFF the instrument. Insert one end of the power cable into the power port and the other end into the power socket of the instrument.

Step 5. Connect the power supply
Ensure whether all connecting points are well-connected and switch power ON the instrument. The instrument is now ready for normal use after self-test.

Instrument Description

I. Instrument description

① Sample chamber cover
② Sample rack
③ Operating panel
④ Pull rod
⑤ Cooling fan
⑥ Louver cover| ⑦ Printer port software
⑧ USB(B) for
⑨ USB interface
⑩ Power socket
○11 Power switch
---|---

II. Operating panel
The operating panel of UV-1000/V-1000 serial model visible spectrophotometers is shown as follows:

① LCD display
② Keypad

III. Key description

| Set the parameters
---|---
| Set the wavelength
| Calibrate 100%T/0Abs
| Print the data
| Function key, promoting functions according to display screen
| Roll down the menu options or turn datasheet pages
| Roll up the menu options or turn datasheet pages

Instrument Use

I. Software system
The instrument’s software system is shown as follows:

II. Basic operations

1. Measurement mode selection
   On the main interface, press the corresponding function key to enter the corresponding measurement mode.

2. Wavelength Setting
   On the key pad, press to enter the wavelength setting interface, press or to change the entered wavelength value and press (left) to proceed to the set wavelength value and automatically calibrate 100%T/0Abs.

3. Parameter Setting
   Press key to enter the parameter setting interface, press  or to select the settings or input parameters and press (left) to confirm. 4.

4. Delete the measurement results and stored data
   If the local memory is full and you want to clear all saved data, you can long press the button to delete all data in the interface of date view.

5. Calibrating 100%T/0Abs
   Place the reference into the light path and press key to calibrate 100%T/0Abs.

6. Measuring samples
   Place the sample into the light path and press (left) to measure the sample.

7. Measurement results printing
   On the measurement interface, short press to enter print setting, input the data number you want to print, press (left) to print the corresponding test results.
   Instrument host and Bluetooth printer online tutorial:

1. Turn on the printer switch, the green light continues to flash;
2. Open the instrument host, after the instrument self-test is completed, it can automatically connect with the Bluetooth printer.
3. If the connection is successful, the green indicator light of the printer will be long bright.
4. The operating instrument can be printed!
   The specific operation process of the Bluetooth printer can be viewed in its user manual!! 
   

III. Preparations before measurement

1. Power-ON self-test
   Ensure that the light path is clear and close the sample chamber cover. Turn ON the power switch on the instrument to start self-test. The start window is like this:
   Self-test
   It can self-check the following items: color filter, lamp switch, detector, deuterium lamp, tungsten lamp, wavelength calibration and dark current calibration.
   Self-test error
   If any item has error during self-checking, it will beep and show the error item. You can press any key to continue the process. After the self-test is done, you can go to the system application for further inspections and calibrations. If errors continue, please contact the service team.

2. Preheating
   After self-test, the instrument enters into the preheating state. For accurate measurement, preheating time needs to be 20-30 minutes.

3. Confirm colorimeter cell
   Before moving the sample into the colorimeter cell, confirm the cell is clean and free of residues. If the wavelength to be tested is less than 400nm, please use quartz cell.

IV. Measurement

1. Photometric measurement
   Photometric measurement is used to test the absorbance or transmittance at a fixed wavelength.
   Step 1. Enter the photometric measurement interface
   After the self-checking and preheating, the device will go to the main interface of photometric measurement automatically as follows.
   You can select the working mode by pressing key and (left) to enter the working mode interface. Then press or to choose working
   mode, like absorbance, transmittance or energy. Then confirm and press (right) to back to main interface.
   On the “main interface”, press (left) to select “Basic”
   Step 2. Set the wavelength to be tested
   Press to enter the wavelength setting interface and  or to input the wavelength value, and press (left) to proceed to the set wavelength value.
   The valid WL range is 200-1000nm, if invalid number is entered, it will beep three times and go back to the main interface of photometric measurement.
   Step 3. Calibrate 100%T/0Abs
   Place the reference into the light path and wait until the stability indicator shows up, then press to calibrate 100%T/0Abs.
   Note: remember to put in the reference sample before doing blanking, otherwise the test results will not be accurate.
   Step 4. Measure the samples
   Place the sample into the light path, wait until the stability indicator shows up, and press (left) to enter measurement interface, then press (test)
   again to have the test results, and the readings will be displayed in the data list.
   Repeat this operation to complete the sample measurements
   Each page can show three data and you can see other pages via pressing or . In the measurement interface, you can set the wavelength too, but need to do the zero again, otherwise the results will not be accurate.
   Step 5. Print the data
   On the measurement interface, short press to enter print setting, input the data number you want to print, press (left) to print the corresponding test results.
   Step 6. Delete the data
   If the local memory is full and you want to clear all saved data, you can long press the button to delete all data in the interface of date view.

2. Quantitative measurement
   You can create the curve via standard samples with known concentration and use this curve to do quantitative measurement for similar samples. If the K and B are known in advance, you can also set up the curve via coefficient method.
   Step 1. Enter the quantitative measurement interface
   On the “main interface”, press (right) to select “quantitative measurement”.
   Step 2. Establish the standard curve or load the stored standard curve
   There are two ways to establish standard curve available for users to choose, depending upon the actual situation.
   Coefficient method:
   This method is a simple way of quantitative measurement, it is realized by a formula with the absorbance value tested by the spectrophotometer.
   
   1) Select the coefficient method.
   Press or to select “create curve” and press  or to select “coefficient method”.
   2) Set the wavelength to be measured.
   Press  or to enter the measured wavelength value and pres (left) to confirm and back to previous interface.
   3) Set the measurement coefficient K.
   Press  or to enter the K value and press (left) to confirm  and back to previous interface.
   4) Set the measurement coefficient B.
   Press  or to enter the B value and press (left) to confirm and back to previous interface.
   Continue to press  or until Keypad become OK, press (left) to confirm and complete the coefficient method setting. Now instrument displays the newly established curve. Then you can do the measurements.
   Note: before do the tests, make sure do the blanking with your reference sample first, otherwise the results will not be accurate.
   Standard Sample Method
   1) Select the standard sample method.
   Press  or to select “create curve”, press press  or to select “standard Curve” and press (left) to enter standard sample method.
   2) Build new standard curve.
   Enter the wavelength and number of samples (up to 9), press (left) to confirm and enter the next interface. Press  or to input the concentration of each sample via keypad. Then press to do blanking, after that, press (left) read the absorbance value of each sample. The details are as follows.
   The standard curves established after successive completion of calibration of all standard samples will be automatically saved in the instrument’s memory.
   Note: the number range of the samples is 1-9. If invalid number is entered, it will beep three time and back to setting interface.
   Invoke the stored standard curve:
   The newly built curves will be saved to the local memory automatically and uses can invoke the directly. It can save up to 50 curves and the latest curve is at the end of the list.
   1) Select the saved curve.
   Press  or to select “Load Curve”, press  or to select the corresponding curve equation and then press (left) to confirm
   2) Enter the sample measurement interface
   Press (left) to enter the measurement interface after invoking a curve.
   3) Calibrate 100%T/0Abs.
   Place the reference into the light path and press to calibrate 100%T/0Abs.
   4) Measure the sample using the standard curve.
   Place the sample into the light path and press (left) to measure it. The results will be displayed in the data list. Repeat this operation to complete measurement of all samples.
   5) Print the data
   On the measurement interface, short press to enter print setting, input the data number you want to print, press (left) to print the corresponding test results.
   6) Delete the data
   If the local memory is full and you want to clear all saved data, you can long press the button to delete all data in the interface of date view.
   Remove curve:
   This is used to delete the saved curves. You can go to this menu and select the curve needs to be removed, and press (left) to delete the curves.
   

V. System Setting
Users may set the system configuration of the instrument in the “system application” depending upon actual use. On the “main interface”, press key to enter the setting parameter interface.

1. Measurement mode
   Press  or to select “Test Mode”, press (left) to enter, press  or to select “absorbance”, “transmittance” or “energy” and then press (left) to confirm.

2. Turn ON and OFF the deuterium lamp
   When measuring with testing wavelength within the range of 3401000nm, users can turn OFF the deuterium lamp to extend the service life of deuterium lamp.
   Press or to select “D2 Lamp”, press (left) to enter, press  or to select “ON” or “Off” and then press (left) to  confirm.
   

3. Turn ON and OFF the tungsten lamp
   When measuring with testing wavelength within the range of 200339nm, users can turn OFF the tungsten lamp to extend the service life of tungsten lamp.
   Press  or  to select “D2 Lamp”, press (left) to enter, press  to select “ON” or “Off” and then press  or (left) to confirm.
   

4. Dark Current Calibration
   When the working environment of the instrument changes, users may recalibrate the dark current after the instrument preheating and before sample measurement.
   Press  or to select “Dark Current”, press (left) to calibrate.
   The sample chamber cover must be closed throughout the entire calibration process.
   Return after completion.
   

5. Wavelength calibration
   Sudden power failures while the instrument is in use, abnormal power OFF and attenuation of light source energy will all result in wavelength deviation and deviation of measurement results, where wavelength recalibration is required.
   Make sure that the light path is clear and the sample chamber cover is closed. Press or to select “Reset WL” and press (left) to calibrate. The sample chamber cover may not be opened throughout the calibration process. Return after completion.
   

6. Version information
   Press or to select “version”, press (left) to view the version information interface and then press any key to return.
   

7. Export data to USB
   You can transfer the saved data on the spectrophotometer to the USB. Press or to select “Export”, press (left) to transfer data.
   Before export the data, you must insert the USB to the USB port on the back of the device, otherwise it will give error information.
   

8. Date and Time Setting
   Press or to select “Time Setting”, press (left) to enter the menu and set the correct date and time.
   

FCC Statement
This device complies with part 15 of the FCC rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
NOTE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to par t 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and receiver.
• Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.
• Consult the dealer or an experienced radio/TV technician for help.

Important Note:
Radiation Exposure Statement
This equipment complies with FCC radiation exposure limits set forth for an uncontrolled environment. This equipment should be installed and operated with minimum distance 20cm between the radiator and your body.
This transmitter must not be co-located or operating in conjunction with any other antenna or transmitter.

ISED Statement
This device complies with Industry Canada license‐exempt RSS standard(s).
Operation is subject to the following two conditions: (1) This device may not cause interference, and (2) This device must accept any interference, including interference that may cause undesired operation of the device.
The digital apparatus complies with Canadian CAN ICES‐3 (B)/NMB‐3(B).
This radio transmitter has been approved by Industry Canada to operate with the antenna types listed with the maximum permissible gain indicated. Antenna types not included in this list, having a gain greater than the maximum gain indicated for that type, are strictly prohibited for use with this device.

Radiation Exposure Statement
This equipment complies with Canada radiation exposure limits set forth for an uncontrolled environment. This equipment should be installed and operated with minimum distance 20cm between the radiator & your body.

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