DLAB HiPette Mechanical Pipette User Manual

Mechanical Pipette
HiPette
User Manual

12301725
Ver.20211213

YOUR NEW PIPETTE

Your new mechanical pipette is a general purpose pipette for the accurate and precise sampling and dispensing of liquid volumes. The pipettes operate on the air displacement principle and disposable tips.
The pipettes cover a volume range from 0.1µL to 10mL.
All pipettes have been quality tested according to ISO8655/DIN12650. The quality control according to ISO8655/DIN12650 involves gravimetric testing of each pipette with distilled water (quality 3, DIN ISO 3696) at 22℃ using the manufacturer’s original tips.
1.1Adjustable volume pipettes

1.2Fully autoclavable
HiPette can be fully autoclavable under 121℃，1atm for 20 mins, Wrap the pipette by gauze before autoclave. It must be left for 12 hours to dry before use. Greasing both sealing ring and piston after every 10 times autoclaving.

UNPACKING

The pipette packing contains the following items：

• Pipette
• Calibration/ remove tool
• User manual
• Pipette holder
• Tips
• Silicone Grease
• Quality control certificate
• Filter element（0.1ul-2.5ul、0.5ul-10ul volume pipette without filter）

INSTALLING THE PIPETTE HOLDER

For convenience and safety always keep the pipette vertically on its own holder when not in use. When installing the holder, please follow the instruction below:

1. Clean the shelf surface with ethanol.
2. Remove the protective paper from the adhesive tape.
3. Install the holder as described in Figure 2A. (Make sure the holder is pressed against the edge of the shelf.)
4. Place the pipette onto the holder as shown in Figure 2B.

PIPETTE COMPONENTS

PIPETTE OPERATION

5.1Volume setting
The volume of the pipette is clearly shown through the handle grip window. The delivery volume (variable volume pipette only) is set by turning the thumb button clockwise is anticlockwise. When setting the volume, please make sure that:

• The desired delivery volume clicks into place
• The digits are completely visible in the display window
• The selected volume is within the pipette’s specified range

Using excessive force to turn the push button outside the range may jam the mechanism and damage the pipette.
5.2Range Lock
Press the range lock to lock the range.
One Key Range Lock:
Press down the range lock to lock the range; lift up the range lock to unlock the range. Prevent pipetting errors caused by touching the adjusting knob by mistake.
5.3Sealing and ejecting tips
Before fitting a tip makes sure that the pipette tip cone is clean. Press the tip on the Fig.4
cone of the pipette firmly to ensure an airtight seal. The seal is tight when a visible sealing ring forms between the tip and the black tip cone.
Each pipette is fitted with a tip ejector to help eliminate the safety hazards associated with contamination. The tip ejector needs to be pressed firmly downwards to ensure proper tip ejection. Make sure the tip is disposed of into a suitable waster container.

PIPETTING TECHNIQUES

6.1Forward pipetting
Make sure that the tip is firmly attached to the tip cone. For best results the thumb button should be operated slowly and smoothly at all times, particularly with viscous liquids.
Hold the pipette vertically during aspiration. Make sure that the liquid and container vessel are clean and that the pipette, tips and the liquid are at the same temperature.

1. Depress the thumb button to the first stop .
2. Place the tip just under the surface of the liquid (2-3mm) and smoothly release the thumb button. Carefully withdraw the tip from the liquid, touching against the edge of container to remove excess.
3. Liquid is dispensed by gently depressing the thumb button to the first stop. After a short delay continue to depress the thumb button to the second stop . This procedure will empty the tip and ensure accurate delivery.
4. Release the thumb button to the ready position .If necessary change the tip and continue with pipetting.

6.2Reverse pipetting
The reverse technique is suitable for dispensing liquids that have a tendency to foam or have a high viscosity. This technique is also used for dispensing very small volumes when it is recommended that the tip is first primed with the liquid before pipetting. This is achieved by filling and emptying the tip.

1. Depress the thumb button all the way to the second stop. Place the tip just under the surface of the liquid (2-3mm) and smoothly release the thumb button.
2. Withdraw the tip from the liquid touching against the edge of the container to remove excess.
3. Deliver the preset volume by smoothly depressing thumb button to the first stop. Hold the thumb button at the first stop. The liquid that remains in the tip should not be included in the delivery.
4. The remaining liquid should now be discarded with the tip or delivered back into the container vessel.

PIPETTING RECOMMENDATIONS

• Hold the pipette vertically when aspirating the liquid and place the only a few millimeters into the liquid
• Prerinse the tip before aspirating the liquid by filling and emptying the tip 5 times. This is important especially when dispensing liquids which have a viscosity and density different from water
• Always control the push button movements with the thumb to ensure consistency
• When pipetting liquids at a temperature different from ambient, prerinse the tip several times before use.

STORAGE

When not in use it is recommended that your pipette is stored in a vertical position.

PERFORMANCE TEST AND RECALIBRATION

Each pipette has been factory-tested and certified at 22℃ according to ISO8655/DIN12650. Note: Pipette specifications are guaranteed only with manufacturer’s tips.
9.1Performance test

• Weighting should take place at 20-25℃, constant to ±0.5℃.
• Avoid drafts.

1. Set the desired testing volume of your pipette.
2. Carefully fit tip onto the tip cone.
3. Prerinse tip with distilled water by pipetting the selected volume 5 times.
4. Carefully aspirate the liquid, keeping the pipette vertical.
5. Pipette distilled water into a tared container read the weight in mgs. Repeat at least five times and record each result. Use an analytical balance with a readability of 0.01 mgs. To calculate the volume, divide the weight of the water by its density (at 20℃: 0.9982). This method is based on ISO8655/DIN12650.
6. Calculate the F-value by using the following equation:
   F=∣inaccuracy (μL) ∣+2×imprecision (μL)
   Compare the calculated F-value to the corresponding Fmax user. If it falls within the specifications, the pipette is ready for use. Otherwise check both your accuracy and, when necessary, proceed to recalibration procedure.

9.2Recalibration procedure

1. Pull out the ejector button, use the calibration tool to push off the ejector button cover, and then install the ejector button back to the handle;
2. Insert the calibration tool into the button hole of the ejector, and press the calibration tool down and hold;
3. At this time, it can be calibrated by turning the adjusting knob (the counter is unchanged at this time); turning clockwise to decrease the volume of liquid, and turning counterclockwise to increase the volume of liquid；
4. Retest after adjustment, and the pipetting volume can be within the allowable system error range.

MAINTENANCE

To maintain the best results from your pipette each unit should be checked every day for cleanliness. Particular attention should be paid to the tip cone (s).
The pipettes have been designed for easy in-house service. However, we also provide complete repair and calibration service including a service report and performance certificate(s). Please return your pipette to your local representative for repair or recalibration. Before returning please make sure that it is free from all contamination. Please advise our Service Representative of any hazardous materials which may have been used with your pipette.
Note: Check the performance of your pipette regularly e.g. every 3 months and always after in-house service or maintenance.
10.1Cleaning your pipette
To clean your pipette using ethanol and a soft cloth or lint-free tissue. It is recommended to clean the tip cone regularly.
10.2In-house maintenance

1. Pull down the ring tube；

2. Unscrew the nozzle connector directly counterclockwise；

3. Remove the piston and spring；

4. Wipe the piston and nozzle connector with alcohol and a lint-free cloth；

5. Before installing nozzle connector it is recommended to grease the Y-shaped sealing ring slightly using the silicone grease provided.
   Note: Excessive use of grease may jam the piston.

6. After reassembling use the pipette (without liquid) several times to make sure that he grease is spread evenly.

7. Check the pipette calibration.

TROUBLE SHOOTING

Leakage or
pipetted volume too small| Tip incorrectly attached| Attach firmly
Unsuitable tip| Use original tips
Foreign particles between tip and tip cone| Clean the tip cone, attach new tip
Instrument contaminated or insufficient amount of grease on Y-shaped sealing ring| Clean and grease Y-shaped sealing ring, clean the tip cone Grease accordingly
Incorrect operation| Follow instruction carefully
Calibration altered or
unsuitable for the liquid| Recalibrate according to instructions
Instrument damaged| Send for service
Push button jammed or moves erratically| Piston contaminated
Penetration of solvent vapours| Clean and grease Y- shaped sealing ring, clean the tip cone
Clean and grease Y- shaped sealing ring, clean the tip cone
Pipette blocked aspirated volume too small| Liquid has penetrated tip cone and dried| Clean and grease Y- shaped sealing ring, clean the tip cone
Tip ejector jammed or moves erratically| Tip cone and/or ejector collar contaminated| Clean the tip cone and the ejector collar

WARRANTY INFORMATION

The pipettes are warranted for one year against defects in materials and workmanship. Should it fail to function in any period of time, please contact your local representative immediately. The warranty will not cover defects caused by normal wear or by using the pipette against the instructions gives in this manual.
Each pipette is tested before shipping by the manufacturer. The Quality Assurance Procedure is your guarantee that the pipette you have purchased is ready for use.

SPECIFICATIONS

Single-channel Adjustable Volume Pipettes (10 models, covering 0.14-10mL.)

VoLume Range| Increment| Test VoLume| Systematic Error| Random Error
%| jiL| %| la
0.1 -2.5uL| 0.002uL| 2.5uL| 1.%| 0.04| 0.7%| 0.02
1.25uL| 3.%| 0.03| 2.%| 0.02
0.25uL| 12.%| 0.03| 6.%| 0.02
0.5-10uL| 0.01uL| lOuL| 1.%| 0.10| 0.4%| 0.04
5uL| 2.%| 0.08| 0.8%| 0.04
luL| 3.%| 0.03| 2.%| 0.02
2-20uL| 0.02uL| 20uL| 1.%| 0.20| 0.3%| 0.06
1 OuL| 1.%| 0.12| 0.6%| 0.06
?AIL| 5.%| 0.10| 2.%| 0.03
5-50uL| | 50uL| 0.9%| 0.45| 0.3%| 0.15
0.05uL| 25uL| 1.%| 0.35| 0.8%| 0.2
| 5uL| 3.%| 0.15| 2.%| 0.08
10-100uL| 0.1uL| 100uL| 0.8%| 0.80| 0.2%| 0.20
50uL| 1.%| 0.50| 3%| 0.15
lOuL| 3.%| 0.30| 1.%| 0.10
20-200uL| 0.2uL| 200uL| 0.6%| 1.20| 0.2%| 0.40
100uL| 1.%| 1.00| 0.3%| 0.30
20uL| 3.%| 0.50| 0.7%| 0.14
30-300uL| 0.2uL| 300uL| 0.6%| 1.80| 0.2%| 0.60
150uL| 1.%| 1.50| 0.3%| 0.45
30uL| 3.%| 0.75| 0.7%| 0.21
100-1000uL| 1 uL| 1000uL| 0.6%| 6.00| 0.2%| 2.00
500uL| 1%| 5.00| 0.2%| 1.00
100uL| 3%| 3.00| 0.6%| 0.60
1000-5000uL| 5uL| 5000uL| 0.6%| 30.00| 0.2%| 10.00
2500uL| 0.6%| 15.00| 0.3%| 7.50
| | 1000uL| 1.20%| 12.00| 0.30%| 3.00
2000-10000uL| 10uL| 10000uL| 0.60%| 60.00| 0.20%| 20.00
5000uL| 0.80%| 40.00| 0.20%| 10.00
2000uL| 1.50%| 30.00| 0.30%| 6.00

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