FLUIGENT Droplet Starter Pack User Guide
- June 11, 2024
- FLUIGENT
Table of Contents
FLUIGENT Droplet Starter Pack User Guide
Content in this pack
1 Droplet Kit
3 EZ Drop chip
1/32″ tubing
2 Adaptor sleeve
2 Flow EZTM
1 Flow EZTM supply kit
2 Flow Unit
2 Low flow rate kit
2 P-Cap
2 P-Cap kit
1 Tube cutter
This guide may also be used by Drolet Kit user for information purpose.
Datasheet is available at
https://www.fluigent.com/product/dropletstarterpack/
For any question: contact@fluigent.com /
www.fluigent.com
Chip overview
The EZ Drop is a microfluidic chip to generate water in oil droplets (using flow focusing). Each chip contains 3 identical devices and each one can be used independently.
Setup installation
Preparation
- Install the whole setup as shown below (Use of filtered solutions will enhance chip life).
- Power on your Flow EZs and follow the first-time use procedure (See Flow EZ’s quick start guide).
Chip connection tips
- Make sure the 1/32″ tubing you are about to insert has a straight clean cut at its end. If not so, use a tube cutter to make a straight clean cut.
- Insert the tubing gently until it touches the bottom.
- Pull the tubing up gently for about 1mm (to leave space for the flow).
- Repeat previous steps for the two other connection holes on the same device.
- If you need to achieve a specific size and rate of droplets, adjust the pressures to reac certain Oil/Water flow rate combination (flow rate is shown as Qmeas on your Flow EZ’s You can use the diagram below to quickly identify the Oil/Water flow rate combination desired diameter and generating rate.
Droplets’ rate/size diagram (part)
Only the part under 1.0 µL/min is presented here
Example of use: For a combination of 1.0 µL/min of oil and 1.0 µL/min of water, you may achieve a droplet diameter of 55 µm at a rate of 190 Hz (11400 droplets per minute).
This diagram shows only the part of low flow rate. The EZ Drop chip itself ca1.n0 achieve up to 4000 Hz under certain circumstances (See the0d.5atasheet fo0.r75more details).
Results may be different from data on this diagram, depending on experimental environment.
