HANNA instruments HI5221 pH/mV Temperature Bench Meters Instruction Manual
- June 11, 2024
- HANNA instruments
Table of Contents
HI5221 pH/mV Temperature Bench Meters
Product Information: HI5221 & HI5222 pH/mV/ISE/Temperature
Bench Meters
The HI5221 and HI5222 are pH/mV/ISE/Temperature bench meters
manufactured by Hanna Instruments. These meters are designed to
provide accurate measurements of pH, mV, ISE, and temperature in
various laboratory applications. The meters come with a liquid
crystal display (LCD) and capacitive touch keypad for easy
operation. They also have USB connectivity for data transfer and
recording.
Specifications:
-
Range: -2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000
pH -
Resolution: 0.1 pH / 0.01 pH / 0.001 pH, 0.1 mV
-
Relative mV offset range pH Calibration
-
ISE Calibration
-
Temperature User Calibration Input Channel
-
Temperature Compensation GLP
-
Logging Record Logging Interval Logging Type
-
pH Electrode Temperature Probe
-
PC Interface Input Impedance
-
Display Power Supply Enviroment Dimensions Weight
-
Up to five points calibration, 8 standard buffers available (pH
1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45), and 5 custom
buffers -
Up to five points calibration, seven fixed standard solutions
available, for each measurement unit, and five custom
solutions -
1 pH/mV, 2 pH/mV/ISE
-
Manual or Automatic
Product Usage Instructions:
-
Remove the instrument from the packing material and examine it
carefully to make sure that no damage has occurred during
shipping. -
If there is any damage, please contact your local Hanna
Instruments Office. -
Connect the power adapter to the power socket of the
meter. -
Connect the BNC electrode connector for pH/ORP/ISE measurements
to the appropriate channel (Channel 1 for HI5221, Channel 1 or 2
for HI5222). -
Connect the temperature probe socket to the appropriate channel
(Channel 1 for HI5221, Channel 1 or 2 for HI5222). -
Turn on the meter using the ON/OFF switch.
-
Select the desired measurement mode (pH, mV, ISE, or
temperature) using the functional keys on the keypad. -
Perform calibration if necessary using the virtual keys
assigned to the highlighted channel. -
Obtain measurements by placing the electrode/probe in the
sample and reading the values on the LCD display. -
Record and transfer data using the USB connectivity
feature.
INSTRUCTION MANUAL
HI5221 & HI5222
pH/mV/ISE/Temperature Bench Meters
Dear Customer
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument.
This manual will provide you with the necessary information for correct use of
this instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us at
tech@hannainst.com or view our worldwide contact list for a Hanna Instruments
representative near you at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
TABLE OF CONTENTS
PRELIMINARY EXAMINATION ………………………………………………………………………………….. 4 GENERAL
DESCRIPTION …………………………………………………………………………………………. 5 FUNCTIONAL DESCRIPTION
…………………………………………………………………………………….. 6 SPECIFICATIONS
………………………………………………………………………………………………….. 9 OPERATIONAL GUIDE
……………………………………………………………………………………………. 10 DISPLAYING
MODES…………………………………………………………………………………………….. 11 SYSTEM SETUP
……………………………………………………………………………………………………. 15 pH SETUP
………………………………………………………………………………………………………….. 21 mV SETUP
…………………………………………………………………………………………………………. 35 ISE SETUP (HI5222 only)
…………………………………………………………………………………….. 37 pH CALIBRATION
…………………………………………………………………………………………………. 45 pH MEASUREMENT
………………………………………………………………………………………………. 49 mV & Relative mV MEASUREMENTS
…………………………………………………………………………. 51 ISE CALIBRATION (HI5222 only)
…………………………………………………………………………….. 53 ISE MEASUREMENT (HI5222 only)
…………………………………………………………………………. 56 TEMPERATURE CALIBRATION
………………………………………………………………………………….. 63 LOGGING
……………………………………………………………………………………………………………. 64 PC INTERFACE
…………………………………………………………………………………………………….. 68 ADDITIONAL INFORMATION
……………………………………………………………………………………. 69 pH BUFFER TEMPERATURE DEPENDENCE
………………………………………………………………….. 72 ELECTRODE CONDITIONING AND MAINTENANCE
………………………………………………………….. 73 TROUBLESHOOTING GUIDE
……………………………………………………………………………………. 75 TEMPERATURE CORRELATION FOR pH SENSITIVE
GLASS ……………………………………………….. 76 ACCESSORIES
……………………………………………………………………………………………………… 77
3
PRELIMINARY EXAMINATION
Remove the instrument from the packing material and examine it carefully to
make sure that no damage has occurred during shipping. If there is any damage,
please contact your local Hanna Instruments Office. The meters are supplied
complete with:
· HI1131B Glass body Combination pH Electrode · HI7662-W Temperature probe ·
HI7082S Electrolyte Solution · HI76404W Electrode Holder · pH Calibration
Solutions Kit · Capillary dropper pipette · 12 Vdc Power Adapter · Instruction
Manual and Quick Reference Guide · Certificate HI5221-01 and HI5222-01 are
supplied with 12 Vdc/120 Vac adapter. HI5221-02 and HI5222-02 are supplied
with 12 Vdc/230 Vac adapter. Note: Save all packing material until you are
sure that the instrument works properly. Any defective item must be returned
in the original packing with the supplied accessories.
4
GENERAL DESCRIPTION
HI5221 and HI5222 are professional bench meters with a color graphic LCD for
pH (with Calibration Check), ORP (Oxidation Reduction Potential), ISE (HI5222
only) and Temperature measurements. The display can be configured as a single
channel or dual channel display (HI5222 only). Each channel can be configured
as pH, mV, Relative mV or ISE (HI5222 only). The main features of the
instruments are: · Single (HI5221) or two (HI5222) input channels; ·
Capacitive touch keypad; · pH calibration using up to five pH buffers: choose
from the list of Hanna Instruments buffers
(pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) or custom buffers (up
to 5 custom buffers); · ISE calibration using up to five standards: choose
from the list of nominal standard values (e.g. for ppm: 0.010, 0.100, 1.00,
10.0, 100, 1000, 10000 ppm) and/or from 5 user-supplied custom standard values
(up to 5 custom solutions); · AutoHold feature to freeze first stable reading
on the LCD; · Two selectable alarm limits; · User Selectable logging modes:
Automatic logging, Manual logging with or without AutoHold; · Continuous Lot
logging directly on meter, with selectable log interval: Store up to 100,000
total data points; · Up to 100 logging lots; a single lot can have up to
50,000 data points; HI5222 (same for each measurement channel). Log files
include GLP information; · Basic Measurement can be viewed with detailed GLP
information, or with a Graph or a Log History; · User-friendly interface on
large color graphic LCD (240 x 320 pixels) with user selectable color palette;
· PC interface via USB; download logged data to PC or use for Real time
logging (HI92000 PC application required); · Dedicated Help key with
contextual message; · Profile feature: store up to ten different user setup
(HI5222 – five on each channel).
5
FUNCTIONAL DESCRIPTION
HI5221 DESCRIPTION FRONT PANEL
REAR PANEL
- Liquid Crystal Display (LCD) 2) Capacitive touch keypad 3) ON/OFF switch 4) Power adapter socket 5) USB connector 6) BNC electrode connector for pH/ORP measurements 7) Temperature probe socket 8) Reference input socket 6
FUNCTIONAL DESCRIPTION
HI5222 DESCRIPTION FRONT PANEL
REAR PANEL
- Liquid Crystal Display (LCD) 2) Capacitive touch keypad 3) ON/OFF switch 4)
Power adapter socket 5) USB connector 6) BNC electrode connector for
pH/ORP/ISE measurements (Channel 1) 7) Temperature probe socket (Channel 1) 8)
Reference input socket (Channel 1) 9) Reference input socket (Channel 2) 10)
Temperature probe socket (Channel 2) 11) BNC electrode connector for
pH/ORP/ISE measurements (Channel 2)
7
FUNCTIONAL DESCRIPTION
KEYBOARD DESCRIPTION FUNCTION KEYS
To enter/exit calibration mode.
To select the desired measurement mode, pH, mV, Rel mV (or ISE HI5222 only).
To enter Setup (System, pH, mV or ISE) and to access Log Recall function.
To obtain general information about the selected option/operation.
VIRTUAL KEYS
The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow
you to perform the displayed function, depending on the current menu (e.g. for HI5222
,
and
in Measure mode).
Note: All the virtual keys are assigned to the highlighted channel (HI5222 only).
LCD GENERAL DESCRIPTION
8
SPECIFICATIONS
Range
Resolution
Accuracy @25 °C / 77 °F
Relative mV offset range pH Calibration
ISE Calibration
Temperature User Calibration Input Channel
Temperature Compensation GLP
Logging Record Logging Interval Logging Type
pH Electrode Temperature Probe
PC Interface Input Impedance
Display Power Supply Enviroment Dimensions
Weight
HI5221
HI5222
-2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000 pH
±2000.0 mV
–
e.g. 10-7 to 10 M, 0.005 to 105 ppm, 5·107 to 5·107 conc.
-20.0 to 120.0 °C / -4.0 to 248.0 °F / 253.2 to 393.2 K
0.1 pH / 0.01 pH / 0.001 pH
0.1 mV
–
1, 2, 3 significants digit
0.1 °C / 0.1 °F / 0.1 K
±0.1 pH / ±0.01 pH / ±0.002 pH ±1 last significant digit
±0.2 mV ±1 last significant digit
–
±0.5% (monovalent ions) ±1% (divalent ions)
±0.2 °C / ±0.4 °F / ±0.2 K
±2000.0 mV
Up to five points calibration, 8 standard buffers available (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45), and 5 custom buffers
Up to five points calibration,
–
seven fixed standard solutions available, for each measurement unit,
and five custom solutions
3 points (0, 50, 100 °C)
1 pH/mV
2 pH/mV/ISE
Manual or Automatic
Electrode offset / slope, calibration points, calibration time stamp, date, time and buffers/standards used
Up to 100 lots, 50,000 records max/lot / maximum 100,000 data points/channel
14 selectable between 1 second and 180 minutes
Automatic, Manual and Auto Hold
HI1131B
HI7662-W
Opto-isolated USB 1012
Color Graphic LCD (240 x 320 pixels)
12 Vdc adapter
0 – 50 °C / 32 – 122 °F / 273 – 323 K max. 95% RH non-condensing
160 x 231 x 94 mm (6.3 x 9.1 x 3.7″)
1.2 Kg (2.6 lbs.)
9
OPERATIONAL GUIDE
POWER CONNECTION Plug the 12 Vdc adapter into the power supply socket.
Notes: These instruments use non-volatile memory to retain the pH, ISE
calibrations and all other settings, even when unplugged. Make sure a fuse
protects the main line. ELECTRODE AND PROBE CONNECTIONS For pH or ORP
measurements, connect a pH/ORP electrode with internal reference to the BNC
connector located on the rear panel of the instrument (to the desired channel
– HI5222 only). For ISE measurements, (HI5222 only) connect, to the desired
channel, an ISE electrode with internal reference to the BNC connector located
on the rear panel of the instrument. For electrodes with a separate reference,
connect the electrode’s BNC to the BNC connector and the electrode’s reference
to the reference input socket. For temperature measurement and automatic
temperature compensation, connect the temperature probe to the appropriate
socket (to desired channel – HI5222 only). INSTRUMENT START UP · Please ensure
that the capacitive keypad is not covered by hand or other objects at the
meter power on. · Turn the instrument on from the power button located on the
rear panel of the instrument. · Please wait until the instrument finishes the
initialization process. Note: It is normal for the loading process to take a
few seconds. If the instrument doesn’t display the next screen, restart the
meter using the power button. If the problem persists, contact your local
Hanna Instruments Office.
10
OPERATIONAL GUIDE
CHANNEL SELECTION (HI5222 only)
· Press
while in Measure mode to access channel
selection menu.
Four available options will be displayed: Channel
1, Channel 2, or multi-channel with the first or
the second channel focused. The “Choose Channel
Configuration” message is displayed in the Reminder
messages area.
· Select the desired option by pressing the appropriate
key:
,
,
or
.
The instrument will display in the selected option Measure mode.
For each measurement mode (pH, mV, Rel mV or ISE) the following display configurations are available: Basic, Good Laboratory Practice (GLP) (pH, ISE only), Graph and Log History.
Basic
The main measured value and it’s units are displayed on the LCD, along with the temperature
value, temperature probe status and basic calibration information when this option is accessed.
To choose the Basic display mode:
· Press
while in Measure mode. The “Choose Display Configuration” message will be
displayed in the Reminder messages area.
· Press
. The instrument will display the basic information for the selected measurement
mode.
DISPLAYING MODES
11
DISPLAYING MODES
GLP
Detailed GLP data will be displayed on the LCD for pH Measure and ISE Measure modes only when this option is selected: Last Calibration date and time, Offset and Slope values, Calibration Buffers/Standards and general information regarding the buffers/standards: the calibration temperature, temperature compensation mode, date and time. For pH Measure, the Electrode Condition is also displayed on the LCD in percent.
Note: If a one point pH calibration is performed or the current calibration does not include at
least two consecutive standard buffers of pH 4.01, 7.01 (6.86) and 10.01 (9.18) buffers, the
Electrode Condition will be unknown. Electrode condition remains active for 24 hours after a
calibration.
To access the GLP display option:
· Press
while in Measure mode. The “Choose Display Configuration” message will be
displayed in the Reminder messages area.
· Press
. The instrument will display the detailed GLP data.
12
DISPLAYING MODES
Graph
The on-line graph with currently logged values (pH, mV, Rel mV, or ISE vs. Seconds) will be
displayed when this option is selected.
If there is no active log, the previously logged data for the selected parameter will be shown.
To access the off-line / on-line graph:
· Press
while in Measure / Logging mode. The “Choose Display Configuration” message
will be displayed in the Reminder messages area.
· Press
. Press
to begin an online graph.
To Zoom Graph
· Press
then
.
and
will appear in virtual keys.
· Use
and
to move along the X (Time) axis of the graph.
· Press
to access the zoom menu for Y axis. Use
or
for zooming Y
(parameter) axis.
· Press
to return to the main menu.
When the off-line graph is displayed:
· Use the arrow keys to move along the X (Time) and Y (parameter) axes of the graph.
· Press
to access the zoom menu for X and Y axes. Use
or
/
/
/
to switch between the active zooming axes. Press
or
to
zoom the selected axis.
Note: While in zoom graph menu the
key is not accessible.
Press
to return to the main menu.
13
DISPLAYING MODES
Log History
The measurement, along with Log History, will be visible when this option is selected:
-
The last stored logged data (Not actively logging) or
-
The last data logged from an active logging lot or
-
An empty display – NO LOTS saved, NOT currently logging
The log history list also contains the appropriate mV values, the logged temperature, the temperature probe status source, as well as the records time stamp.
To access the Log History display option:
· Press
while in Measure mode. The “Choose Display Configuration” message will be
displayed in the Reminder messages area.
· Press mode.
. The instrument will display the log history regarding the selected Measure
Notes: When an alarm condition is active, all logged records will have an exclamation mark “!” If logged in Auto Hold, logged records will have an “H”.
If another Measure mode is selected, the Log History will reset. If the
temperature unit is changed, all logged temperature values will be
automatically displayed in the new temperature unit.
14
SYSTEM SETUP
The System Setup menu allows the user to customize the user interface, view meter information, set the external serial communication interface and to restore the manufacturer settings.
Accessing System Setup
· Press
while in Measure mode.
· Press
. The system setup options will be
displayed on the LCD.
To access a System Setup option:
· Use
or
to highlight the desired
option.
· Press
to access the selected option.
The following is a detailed description of the System Setup option screen:
Beeper
This option allows the user to turn an acoustic warning signal on or off. This
function can be used to signal 4 different events: a stable signal, an alarm
state, when every key is pressed or when an incorrect key is pressed. Enable
(or disable) the Beeper for these events. Disabling the Beeper will stop
audible signals.
15
SYSTEM SETUP
Saving Confirmation
Enable this option to force confirmation of a change made to a setting in GLP
data option field or a Sample ID name. If Saving Confirmation is enabled, the
user will have to accept the change with a key stroke. If Saving Confirmation
is disabled, the changes made to these fields change automatically without
asking for confirmation
GLP Data
Use this option to customize logging GLP information with specific
identification data. When enabled, these ID tags will be included in the GLP
section of all data logs for all modes of operation and both channels
(HI5222). Each data field can use up to 10 characters. The five available
fields are:
Operator ID : used to add the name of the operator
Instrument ID : used to name an instrument with a discrete name, location or
number
Company Name : used to include the Company ID to the GLP data field.
Additional Info : two data fields are available for general notes or
notations.
To add the GLP Data:
· Press
while in Measure mode.
· Press
.
· Use
or
to select the GLP Data option.
· Press
and use
or
to highlight
the desired option.
· Press
to edit the desired information. The Text
Editor menu will be displayed on the LCD.
· Enter the desired information by accepting the
highlighted character which is added to the text bar,
using
. The
and
keys help the
user to select the desired character. It is also possible to delete the last character by positioning the
cursor on the Backspace character ( ) and pressing
.
16
SYSTEM SETUP
· Press
to return to the GLP Data options. If the Saving Confirmation is enabled, press
to accept the modified option,
to escape without saving or
to return
to the editing mode. Otherwise, the modified options are saved automatically.
Date & Time Set the current date & time and the format in which they appear.
Set Date and Time This option allows the user to set the current date (year/month/day) and time (hour/minute/second).
Notes: Only years starting with 2000 are accepted.
The time is set using the selected time format. For 12 Hour time format only, the AM/PM
can also be selected with
or
.
Set Time Format Choose between 12-Hour (AM/PM) time format or 24-Hour time format.
Set Date Format
Choose the desired date format from 7 available options: DD/MM/YYYY,
MM/DD/YYYY, YYYY/MM/DD, YYYY-MM-DD, Mon DD, YYYY, DD-Mon-YYYY or YYYY-Mon-DD.
To set the Date & Time:
· Press
while in Measure mode.
· Press
.
· Use
or
option.
to select the Date & Time
· Press
and use
or
to
highlight the desired option you want to modify.
· Press
to confirm your selection. Use
/
to select next/previous entry
to be edited. Press
and use
or
to set the desired value, then press
to save the modified value (for Set Date and Time
option). For the Set Date Format option press
to confirm your selection and select one of
the displayed options with
or
.
· Press
to confirm your selection and return to the Date & Time options or press
to cancel operation.
17
SYSTEM SETUP
Note: If the time is changed with more than one hour before last pH/ISE calibration, a pop-up warning will appear on the LCD, notifying the user that a date/time conflict has occurred and some time-dependent modes could work improperly (e.g. Measure, GLP, Log).
LCD Setup
This option allows the user to set the Contrast, the Backlight of the LCD and the Backlight Saver. The Contrast parameter can be adjusted within 7 steps, while the Backlight parameter within 8 steps. The Backlight Saver can be set from 1 to 60 minutes or it can be OFF (disabled). All the changes are visible on the LCD for each parameter.
Note: If the instrument backlight turns off after the set period of time, press any key to turn it back on.
To set the LCD Setup:
· Press
while in Measure mode.
· Press
.
· Use
or
to select the LCD Setup option.
· Press
and use
parameter.
key to highlight the desired
· Use
or
to adjust the contrast / backlight
or to set the desired backlight saver time.
· Press
to confirm the modified options and return
to the System Setup menu.
Color Palette
This option allow the user to choose desired color palette.
To select the Color Palette:
· Press
while in Measure mode.
· Press
.
· Use
or
option.
to select the Color Palette
Color 1 White background blue text
Color 2 Blue background white text
Color 3 White background black text
Color 4 Black background white text
18
SYSTEM SETUP
· Press
and use
or
to highlight the desired color.
· Press
to confirm your selection and return to the System Setup menu or press
to return to the System Setup menu without changing.
Language
This option allows the user to choose the desired language in which all information will be displayed.
To select the Language:
· Press
while in Measure mode.
· Press
.
· Use
or
option.
to select the Language
· Press
and use
or
to
highlight the desired language.
· Press
to confirm your selection and return to
the System Setup menu or press
to return to
the System Setup menu without changing.
Serial Communication
This option allows the user to set the desired speed for the serial communication (baud rate) in bps. The meter and the PC program must have the same baud rate.
To set the Serial Communication:
· Press
while in Measure mode.
· Press
.
· Use
or
to select the Serial
Communication option.
· Press
and use
or
to
highlight the desired baud rate.
· Press
to confirm your selection and return to
the System Setup menu or press
to return to
the System Setup menu without changing.
19
SYSTEM SETUP
Meter Information
This option provides general information about the instrument serial number (each instrument has a unique identification serial number), the software version and the factory calibration date and time (for mV and temperature).
Note: All instruments are factory calibrated for mV and temperature. One year after factory calibration, a warning message “Factory Calibration Expired” will be displayed when powering up the instrument. The instrument will still function, however, it should be taken to the nearest Hanna Instruments Customer Service for factory calibration.
To view the Meter Information:
· Press
while in Measure mode.
· Press
.
· Use
or
to select the Meter
Information option.
· Press
to acces the Meter Information menu.
· Press
to return to the System Setup menu.
Restore Factory Settings
This option allows the user to erase all user settings and reset the instrument to the default factory settings in pH mode.
To restore the Factory Settings:
· Press
while in Measure mode.
· Press
.
· Use
or
Settings option.
to select the Restore Factory
· Press
to confirm your selection. A pop-up
menu will be displayed, asking for confirmation.
· Press
to confirm your selection and return to
the System Setup or press
to return to the
System Setup menu without restoring defaults.
· Press
to return to Measure mode.
20
SYSTEM SETUP
Software update
This function allows the user to update instrument software. In order to start
the PC upgrade application, you need to select the proper baud rate, the
software update package and start the update.
pH SETUP
The pH Setup menu allows the user to set the parameters associated with pH measurement and calibration. These parameters can be set specifically for each channel (HI5222 only). The settings will be applied only to the active channel.
Accessing pH Setup
· Press
while in Measure mode and then
to select pH range for the desired channel.
· Press menu.
and then
to access pH Setup
To access a pH Setup option:
· Use
or
option.
to highlight the desired
· Press
to access the selected option.
The following is a detailed description of the pH Setup option screens.
21
pH SETUP
Profile
This option opens the Profile manager. Enabling Profile allows the user to
Save, Load or Delete an application Profile. The Profile option allows the
user to store up to ten separate profile applications (HI5222 – five profiles
for each channel). Each Profile can be named and recalled at a moment’s
notice. A profile is a sensor setup complete with measurement units, logging
and display preferences, calibration standards (Buffer or Standards including
custom), setup of the Display screen for measurement (i.e. single, dual,
graphing, GLP) and any other sensor configuration. Once saved, the exact same
profile can be used at another time. This is a handy feature if the meter is
used occasionally for additional applications because it saves time in the
setup of the meter and ensures that the same procedure will be used.
To save the measurement configuration for pH mode:
· Press
and use
or
to highlight Profile.
· Press
/
to enable / disable this feature.
The available options are: Save Profile: save the current profile. Save Profile As…: save current profile using a specific name. Load Profile: load from available profiles. Delete Profile: delete a profile.
Save Current Profile
To save the current profile:
· Use
or
to select Save Profile As…
· Press
. The Text Editor box will be displayed on the LCD.
· Enter the desired profile name by using
and
to highlight the desired character
and then press
to add it to the text bar. It is also possible to delete the last character by
positioning the cursor on the Backspace character( )and pressing
.
· Press
to return to the Profile options.
· Use Save Profile to save changes made to a presently used Profile. Changes will overwrite
existing configurations.
22
pH SETUP
· Select Load Profile to select a profile to use from the list of saved profiles. Highlight the desired
profile and press
.
· Select Delete Profile to remove a selected profile from the saved list.
Highlight the profile and press .
Temperature
The temperature has a direct influence on pH. This option allows the user to
choose the temperature source and units, as well as the desired manual
temperature for manual temperature compensation mode. Temperature Source
(HI5222 only)
If using a temperature probe, Automatic Temperature Compensation will be
performed relative to the displayed temperature, with the “ATC” indicator
displayed on the LCD. For the HI5222, one temperature probe can be used for
both measurement channels if desired. Select the source by selecting Manual,
Channel 1 or Channel 2 . If no temperature probe is detected, Manual
Temperature Compensation will be performed, with the “MTC” indicator on the
LCD. Temperature Unit
The desired temperature unit can be chosen (Celsius, Fahrenheit or Kelvin
degrees) and the meter will automatically make the conversion for the selected
unit.
If no temperature probe is connected, the desired temperature can be set
manually. The default setting is 25.0 °C. If the measured temperature is
different, the value can be manually adjusted to obtain an accurate pH
measurement.
To set one of the Temperature options:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Temperature
option.
· Press
and use
or
to highlight
the desired Temperature option you wish to modify.
· Press
and use
or
to highlight
the desired option (for Temperature Source & Unit
options) or use
or
to adjust the
temperature value between the displayed limits (for
Manual Temperature option).
23
pH SETUP
· Press
to confirm your selection (for Temperature Source & Unit options) or press
to save the current value (for Manual Temperature option). Otherwise, press
to cancel
operation.
Calibration This option allows the user to setup desired buffers and calibration reminder.
Buffer Entry Type Three settings are available for the pH buffers used for electrode calibration: Automatic – the instrument automatically selects the closest buffer to the measured pH value from the predefined buffers chosen in the option Edit Buffer Group. Semiautomatic – the instrument automatically selects the closest buffers to the measured pH value from all available buffers and you can choose the one used to be, from standard and custom buffers. Manual Selection – the desired pH buffer is manually selected from all available buffers (standard and custom).
To set the Buffer Entry Type:
· Press
while in pH Measure mode.
· Press
.
· Use
or
option.
to select the Calibration
· Press
and use
or
to
highlight the Buffer Entry Type option.
· Press
and use
or
to
highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
1st Cal. Point Two options are available for the 1st Cal. Point parameter:
Point and Offset. Point: A new buffer can be added to an existing calibration.
The electrode slope will be reevaluated with the addition of this buffer
(normal operation). Offset: The new buffer calibration point can create a
constant offset to all existing pH calibration data (existing calibration must
have a minimum of two pH buffers).
24
pH SETUP
To set the 1st Cal. Point:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Calibration
option.
· Press
and use
or
to
highlight the 1st Cal. Point option.
· Press
/
as desired.
· Press
to return to previous menu.
Edit Custom Buffers
If special custom pH buffers are required during calibration, the Edit Custom Buffers option is available. Up to five pH custom buffers can be added. If a custom buffer is used, the user must verify it’s value at temperature of calibration.
To edit/set the Custom Buffers:
· Press
while in pH Measure mode.
· Press
.
· Use
or
option.
to select the Calibration
· Press
and use
or
to
highlight the Edit Custom Buffers option.
· For a previous set value, press confirm the setting by pressing buffer.
to set the custom buffer value to “—-” if desired and
, otherwise press
to edit the selected custom
· While in edit custom buffer menu press
to set the custom buffer value to 7.000 pH and
then use
or
to set the desired custom buffer value.
25
pH SETUP
· Press
to exit custom buffer edit menu. If the Saving Confirmation is enabled, press
to accept the modified option,
to escape without saving or
to return
to the editing mode. Otherwise, the modified option is saved automatically.
· Use
key to select the next custom buffer to be set or press
to return to Calibration
options.
Edit Buffer Group
Accessing this option, the user can edit the desired group of five pH buffers for automatic buffer recognition (Automatic Buffer Entry Type). If the Buffer Group already contains five pH buffers, at least one pH buffer has to be removed in order to add another buffer.
To edit/set the Buffer Group:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Calibration option.
· Press
and use
or
to highlight
the Edit Buffer Group option.
· Press
and use
and
to choose the pH buffer to be included in the buffer
group.
· Press
or
to add/remove the selected pH buffer to/from the buffer group.
· Press
to return to Calibration options and to save the changes.
Calibration Reminder
This option allows the user to select a calibration reminder schedule if desired. Three options are available for the calibration reminder: Daily, Periodic or Disabled.
To set the Calibration Reminder:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Calibration option.
26
pH SETUP
· Press · Press · Press
and use
or
to highlight the Calibration Reminder option.
and use
or
to highlight the desired option.
to confirm your selection or press
to cancel operation.
Set Reminder Period Schedule the calibration reminder timing with this option (verify Daily or Periodic is set for Calibration Reminder). If a Daily reminder is desired, set the time of day you wish the reminder to occur. If a Periodic reminder is desired, schedule time in days, hours and/or minutes after the last calibration for the reminder to occur. To set the Reminder Period:
· Press
while in pH Measure mode.
· Press
.
· Use
or
option.
to select the Calibration
· Press
and use
or
to highlight the Set Reminder Period option.
· Press
and use
/
to select next/previous entry to be edited.
· Press
and use
or
to set the desired value, then press
to save
the modified value.
· Press
to return to the Calibration options. If the Saving Confirmation is enabled, press
to accept the modified option,
to escape without saving or
to the editing mode. Otherwise, the modified option is saved automatically.
to return
Clear Calibration
This feature deletes the pH electrode calibration for the attached electrode
and channel (HI5222). A default meter calibration will replace the actual
electrode calibration until a new electrode calibration is made.
27
pH SETUP
To clear Calibration:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Calibration
option.
· Press
and use
or
to
highlight the Clear Calibration option.
· Press
to clear calibration. A pop-up menu
will be displayed asking for confirmation (when a
calibration is available).
· Press
to confirm or press
to escape
without saving and return to the Calibration options.
Sample ID
This option allows the user to assign an identification number/name. Two
Sample ID options are available: ID Increment and Edit Sample ID.
ID Increment
Two choices are available for the sample ID:
None – the sample ID will be fixed and it can be set alphanumeric (see Edit Sample ID).
Automatic – the sample ID will automatically increment by one for each new log lot.
To set the ID Increment mode:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Sample ID option.
· Press
and use
or
to highlight the ID Increment option.
· Press
/
as desired.
· Press
to return to previous menu.
28
pH SETUP
Edit Sample ID
This option allows the user to edit the sample ID. Note: The ID Increment mode must be set to None, to use this feature.
To edit the Sample ID:
· Press
while in pH Measure mode.
· Press
.
· Use
or
option.
to select the Sample ID
· Press
and use
or
to
highlight the Edit Sample ID option.
· Press
to confirm your selection.
· If the selected increment is None, the Text Editor menu will be displayed on the LCD, allowing you
to enter the desired sample number/name by accepting the highlighted character which is added
in the text bar, using
. The
and
keys help the user to select the desired
character.
It is also possible to delete the last character; position the cursor on the Backspace character and
press
.
· Press
to return to Sample ID options. If the
Saving Confirmation is enabled, press
to accept the modified option,
to escape
without saving or
to return to the editing
mode.
Otherwise, the modified options are saved
automatically.
· If the selected increment mode is Automatic, the desired
sample ID value can be set using
or
· Press
to save the current value or press
to cancel operation.
29
pH SETUP
Stability Criteria
This option allows the user to select the signal stability criteria for the measured parameter (pH, mV, ISE):
Fast – this setting will give faster results with less accuracy.
Medium – this setting will give medium speed results with medium accuracy.
Accurate – this setting will give slower results with high accuracy.
To set the Stability Criteria:
· Press
while in pH Measure mode.
· Press
.
· Use
or
Criteria option.
to select the Stability
· Press
and use
or
to
highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
Reading Mode
This option allows the user to select between Direct and
Direct/AutoHold pH reading mode. If choosing the AutoHold option, the current reading can be frozen
on the LCD when
is pressed and the stability criteria is reached.
To set the Reading Mode:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Reading Mode
option.
· Press
/
to select Direct / AutoHold
option as desired.
· Press
to cancel operation.
30
pH SETUP
Log Note: See section Logging for available types of logging.
This option allows the user to edit the logging settings: Logging Type,
Logging Data Configuration, Sampling Period and New Lot.
Logging Type
Three logging types are available: Automatic, Manual and Auto Hold.
Automatic – the measurement data is logged automatically at predefined time intervals;
Manual – a snapshot of the displayed measurement data is logged with time stamp when the user manually presses Log;
Auto Hold – this is configured along with the Direct/AutoHold Reading Mode to take a snapshot of
stable measurement data. Press
to initiate a logging session. Press
to initiate an
Auto Hold event. The log occurs automatically once measurement stability is reached. This type log
removes subjective data, as it only captures stable measurements.
To set the Logging Type:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Log option.
· Press
and use
or
to
highlight the Logging Type option.
· Press
and use
or
to
highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
Logging Data Configuration
This option allows the user to select which additional parameters will
accompany a log report: Date/Time, Calibration Data, Sample ID, Instrument ID,
Operator ID, Company Name, Additional Info 1 and Additional Info 2.
31
pH SETUP
To set the Logging Data Configuration:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Log option.
· Press
and use
or
to highlight
the Logging Data Configuration option.
· Press
and use
or
to highlight
the desired parameter to be logged in file.
· Press
to enable the parameter or
to
disable it.
· Press
to return to previous menu.
Sampling Period
This option allows the user to select the desired sampling period for automatic logging type.
To set the Sampling Period:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Log option.
· Press
and use
or
the Sampling Period option.
to highlight
· Press
and use
or
to select the
desired option.
· Press
to confirm your selection or press
to cancel operation.
New Lot This option is used to create a new lot when manual logging is used.
Note: If New Lot option is accessed and the Logging Type is Automatic, a
warning message appears on the LCD informing the user that a new lot can be
created only if the Logging Type is set as Manual.
32
pH SETUP
To generate a New Lot:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Log option.
· Press
and use
or
to highlight the
New Lot option.
· Press
to generate a new manual lot. A pop-up
menu will be displayed asking for confirmation.
· Press
to confirm or press
to escape
without saving and return to the Log options.
Alarm
This option allows the user to select the alarm settings: Alarm State and
Alarm Limits. If the Alarm option is enabled, a continuous double beep will be
heard, along with the “Alarm” indicator blinking on the LCD, each time the set
limits in Measure mode are exceeded.
Note: Alarm Beeper must be set On for audible beep to be heard. See: System
Setup > Beeper > Alarm.
Alarm State
Three settings are available for the Alarm State option:
Disabled – the alarm will be disabled.
Inside Limits – the alarm state will trigger when the measured value is inside the set limits.
Outside Limits – the alarm state will trigger when the measured value is outside the set limits.
To set the Alarm State:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Alarm option.
· Press
and use
or
to
highlight the Alarm State option.
· Press
and use
or
to highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
Alarm Limits
This option allows the user to set the alarm limits for the measured value. Note: The Alarm High value can not be lower than the Alarm Low value.
33
pH SETUP
To set the Alarm Limits:
· Press
while in pH Measure mode.
· Press
.
· Use
or
to select the Alarm option.
· Press
and use
or
to
highlight the Alarm Limits option.
· Press
and use
/
to select
next/previous entry to be edited.
· Press
and use
or
to set the
desired value, then press
to save the modified
value.
· Press
to return to the Alarm options. The
modified option is saved automatically.
Isopotential Point
This option allows the user to edit the isopotential point of the electrode used for pH measurements. The isopotential point is the mV reading for an electrode at which temperature has no effect on the measurement. The ideal electrode has an isopotential point of 0.0 mV and 7.00 pH, while an actual electrode typically deviates slightly from the ideal values.
If the actual isopotential pH for an electrode is known, it can be set by accessing this option.
Note: If the isopotential point has been modified, recalibration must be performed.
To set the Isopotential Point:
· Press
while in pH Measure mode.
· Press
.
· Use
or
Point option.
to select the Isopotential
· Press using
and set the desired isopotential pH value
or
.
· Press
to save the current value or press
to cancel operation.
34
pH SETUP
pH Resolution
Select the desired pH resolution with this option. Choose from one (X.X), two (X.XX) or three (X.XXX) digits displayed past the decimals.
To set the pH Resolution:
· Press
while in pH Measure mode.
· Press
.
· Use
or
option.
to select the pH Resolution
· Press
and use
or
to highlight
the desired option.
· Press
to confirm your selection or press
to cancel operation.
The mV Setup menu allows the user to set the parameters associated with mV and Relative mV measurements. These parameters can be set specifically for each channel (HI5222 only). The settings will be applied only to the active channel.
Accessing mV Setup
· Press
while in Measure mode and then
or
range for the desired channel.
· Press menu.
and then
to access mV Setup
To access a mV Setup option:
· Use
or
option.
to highlight the desired
· Press
to access the selected option.
The following is a detailed description of the mV Setup option screens.
to select mV / Rel mV
mV SETUP
35
mV SETUP
Profile- See pH Setup section.
Temperature ORP measurements are not temperature compensated, although ORP
values can change with temperature (e.g. reference electrode potential
changes, sample equilibrium changes). It is important to report ORP values
together with the reference electrode used and the temperature of measurement.
This option permits selection of the temperature source (HI5222 only) and
measurement units.
Temperature Source If using a temperature probe, sample temperature will be
displayed with the “ATC” indicator displayed on the LCD. For HI5222 only, the
ATC option can be selected from Channel 1 or Channel 2. If no temperature
probe is detected, a Manual set value will be displayed (and logged) with the
measurement.
Temperature Unit Select the desired temperature unit (Celsius, Fahrenheit or
Kelvin degrees) and the meter will automatically convert to the selected unit.
Manual Temperature If no temperature probe is connected, the desired
temperature can be manually entered. The default setting is 25.0 °C.
Calibration (Relative mV only) Calibration Reminder This option allows the
user to select a calibration reminder schedule if desired. See pH Setup
section > Calibration Reminder section for option access details. Set Reminder
Period See pH Setup section > Set Reminder Period section.
36
mV SETUP
Clear Calibration
This feature deletes the Relative mV calibration for the selected channel.
· Press
while in Rel mV mode.
· Press
then use
or
to access Calibration option.
· Press
and use
or
to highlight Clear Calibration option.
· Press
to clear calibration. A pop-up menu will be displayed asking for confirmation
(when a calibration is available).
· Press
to confirm or press
to escape without saving and return to the Calibration
options.
Sample ID – See pH Setup section. Stability Criteria – See pH Setup section. Reading Mode – See pH Setup section. Log – See Logging section, or pH Setup section. Alarm – See pH Setup section.
ISE SETUP (HI5222 only)
The ISE Setup menu allows the user to set the parameters regarding ISE measurement and calibration. These parameters can be set specifically for each channel. The settings will be applied only to the active channel.
Accessing ISE Setup
· Press
while in Measure mode and then
to select ISE range for the desired channel.
· Press menu.
and then
to access ISE Setup
To access an ISE Setup option:
· Use
or
option.
to highlight the desired
· Press
to access the selected option.
The following is a detailed description of the ISE Setup option screens.
37
ISE SETUP (HI5222 only)
Profile – See pH Setup section.
Reading Mode This option allows the user to select the desired reading mode:
Direct, Direct/AutoHold, Known Addition, Known Subtraction, Analyte Addition
and Analyte Subtraction. Four of these Reading Modes are collectively known as
Incremental Methods (see ISE Theory section for details). Direct measurements
and Direct/AutoHold measurements are also available.
Direct Direct measurements are analogous to taking pH measurements. The ISE is
calibrated in ion standards and sample measurements are made directly. The
ISE’s manual should be consulted for tips and practices of making Direct
measurements. The ion concentration can be read directly from the instrument.
Direct/AutoHold Direct/AutoHold measurements are made similar to Direct
measurements. The advantage of using Direct/AutoHold is a measurement that has
not reached equilibrium will not be used. Only after the chosen stability
criteria has been met will the meter go into the Direct/AutoHold mode. Using
Direct/AutoHold removes the subjective nature of stability.
Known Addition In the Known Addition method, a sample is measured with an ISE
before and after the addition of a known volume of a standard. The mV
difference is then used to calculate the concentration of the ion in the
original sample.
Known Subtraction In the Known Subtraction method, a sample is measured with
an ISE before and after the addition of a known volume of a reactant standard.
The reactant standard reacts with the measured ion in the sample, reducing
it’s concentration. The mV difference is then used to calculate the
concentration of the ion in the original sample. The stoichiometric ratio
between reactant standard and ion in the sample must be known.
Analyte Addition Analyte Addition is similar to the Known Addition method,
with the difference being that an aliquot of sample is added to a known volume
of standard. Both solutions contain the same measured ion. The standard is
measured with an ISE before and after the addition of a known volume of a
sample. The ion concentration is then calculated using the difference in mV
potential. The sample should increase the concentration of the ion being
measured.
38
ISE SETUP (HI5222 only)
Analyte Subtraction
In the Analyte Subtraction method, an aliquot of sample is added to a reactant
standard of known concentration and volume. The sample partially reacts with
the measured ion. The stoichiometric ratio between standard and sample must be
known. The ion concentration is then calculated using the difference in mV
potential.
To set the Reading Mode:
· Press
while in ISE Measure mode.
· Press
.
· Use
or
to select the Reading Mode
option.
· Press
and use
or
to
highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
Temperature This option permits the user to configure all parameters related
to ISE temperature measurements.
Temperature Source The options are: Manual, Channel 1 or Channel 2. If no
temperature probe is detected, a manually set value will be displayed (and
logged) with the measurement. If a temperature probe is connected to either
channel, it may be selected. The temperature measurement will be displayed and
logged with the measurement and may be used for temperature compensation
calculation if Temperature Compensation is enabled.
Temperature Unit Select the desired temperature unit (Celsius, Fahrenheit or
Kelvin degrees) and the meter will automatically convert to the selected unit.
Manual Temperature If no temperature probe is connected, the desired
temperature can be set manually. The default setting is 25.0 °C. If the
measured temperature is different, the value can be manually adjusted to
obtain an accurate ion measurement.
39
ISE SETUP (HI5222 only)
Temperature Compensation
ISE measurements benefit from temperature compensated corrections if: ·
standards and sample temperatures differ from each other · the Isopotential
Point of the ISE is known. If sample and standards are made at the same
temperature, leave this option disabled.
Isopotential Point
If the Temperature Compensation is enabled, the isopoten-
tial point of the ISE must be added in this parameter. Verify
the Electrode Type and Concentration Unit are configured
for the desired application. The Isopotential point will use
the selected concentration unit. Use
and
to edit the isopotential point value and press
to
save the value or press
to cancel operation.
Notes: A warning message will appear on the LCD informing the user to perform a new calibration. A minimum of two ion standards is required for the ISE calibration.
Calibration This option allows the user to view and configure all ISE
parameters related to ISE calibration.
Manual Entry Two different standard groups can be used for calibration of ISE:
All Standards – During calibration the user can select the desired standards
from a large list containing all the predefined standard values and the custom
standards. Group Standards – the user can pre-select a group of standards from
the existent group of standards to be used during sensor calibration.
40
ISE SETUP (HI5222 only)
To set the Manual Entry:
· Press
while in ISE Measure mode.
· Press
.
· Use
or
to select the Calibration option.
· Press
and use
or
to highlight the Manual Entry option.
· Press
or
to select the desired option.
Edit Custom Standards
Use Edit Custom Standards function to add additional ISE standard values. Up to five custom standard values can be added. Set Electrode Type and Concentration Unit prior to adding these standards.
To edit/set the Custom Standards:
· Press
while in ISE Measure mode.
· Press
.
· Use
or
option.
to select the Calibration
· Press
and use
or
to
highlight the Edit Custom Standards option.
· If you want to disable the custom standard, press
. A pop-up menu will be displayed
asking for confirmation. Press
to confirm (the custom standard value will turn to “—-“)
or press
to cancel the operation.
· Use
key to select the next custom standard to be set.
· Press
to return to Edit Custom Standard options.
Edit Standard Group
If a Group Standard was selected in the parameter Manual Entry, this parameter is used to create your group of standards. If the Standard Group already contains five ISE standards, at least one ISE standard has to be removed in order to add another standard.
To edit/set the Standard Group:
· Press
while in ISE Measure mode.
· Press
.
41
ISE SETUP (HI5222 only)
· Use
or
to select the Calibration option.
· Press
and use
or
the Edit Standard Group option.
to highlight
· Press
and use
and
to choose
the ISE standard to be included in the standard group.
· Press
/
to add/remove the selected ISE
standard to/from the standard group.
· Press
to return to Calibration options and to
save the changes.
Calibration Reminder – See Calibration option from pH
Setup section.
Set Reminder Period – See Calibration option from pH Setup section.
Clear Calibration – See Calibration option from pH Setup section.
Electrode Type
This option allows the user to select the desired Ion Selective Electrode used
for measurements from a list: Ammonia, Bromide, Cadmium, Calcium, Carbon
Dioxide, Chloride, Cupric, Cyanide, Fluoride, Iodide, Lead, Nitrate,
Potassium, Silver, Sodium, Sulfate, Sulfide and five custom ISE. For the
standard ISE it is possible to view the Ion constants (Name, Molar Weight and
Electric Charge/Slope), while for the custom ISE all these constants can be
manually set.
To set the Electrode Type:
· Press
while in ISE Measure mode.
· Press
.
· Use
or
option.
to select the Electrode Type
· Press
and use
or
to select
the desired standard ISE or a custom one from the list.
For standard ISE:
· Press press mode.
to visualize the Ion Constants and then at any time to exit Ion Constants view
42
ISE SETUP (HI5222 only)
· Press
to confirm your selection and return to ISE Setup options.
For custom ISE:
· Press
to edit the Ion Constants for the selected
custom ISE. Use
or
to select the
desired constant and press
to enter edit mode
or
to cancel operation.
· For the ion Name the Text Editor menu will be
displayed on the LCD. Enter the desired information by
accepting the highlighted character which is added in
the text bar, using
. The
and
keys help the user to select the desired character. It is
also possible to delete the last character by positioning
the cursor on the Backspace character ( ) and
pressing
. Press
to return to the
Ion Constants menu.If the Saving Confirmation is
enabled, press
to accept the modified option,
to escape without saving or
to return
to the editing mode. Otherwise, the modified option is
saved automatically.
· To set the appropriate Ion Molar Weight (in g/mol units)
use
or
to set the desired value and
then press
to save the current value or press
to cancel operation.
· To select the appropriate Ion Charge/Slope use
or
and then press
. If the ion electric
charge is None, its slope can be manually set by
pressing
.
A pop-up menu will be displayed on the LCD, in which the slope value can be set using
or
. Press
to save the modified value or press
to return to the previous
menu.
43
ISE SETUP (HI5222 only)
Note: If an ISE calibration was performed and a different Ion Selective Electrode is selected (standard or custom), a warning message appears on the LCD informing the user to perform a new calibration or to select the previous ISE in order to perform accurate measurements.
Concentration Unit
Select the desired concentration unit for the measured ion or chemical compound. The available
concentration units are: ppt, g/L, ppm, mg/L, µg/mL, ppb, µg/L, mg/mL, M, mol/L, mmol/L,
%w/v and User (custom unit).
To set the Concentration Unit:
· Press
while in ISE Measure mode.
· Press
.
· Use
or
to select the Concentration
Unit option.
· Press
and use
or
to
highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
Sample ID – See pH Setup section. Stability Criteria – See pH Setup section.
Log – See pH Setup section.
Note: The Logging Data Configuration option includes also the Ion Constants
parameter. If you want it to appear in the log reports, it must be enabled.
Alarm – See pH Setup section. Note: The Alarm Limits (Low and High) are set in
the selected concentration unit of the measured
44
ISE SETUP (HI5222 only)
ISE Significant Digits
Accessing this option, the number of ISE significant digits can be set, with one (X), two (XX) or three (XXX) significant digits.
To set the ISE Significant Digits:
· Press
while in ISE Measure mode.
· Press
.
· Use
or
Digits option.
to select the ISE Significant
· Press
and use
or
to
highlight the desired option.
· Press
to confirm your selection or press
to cancel operation.
pH CALIBRATION
Calibrate the instrument often, especially if high accuracy is required. The
instrument should be recalibrated: · Whenever the pH electrode is replaced. ·
At least once a week. · After testing aggressive chemicals. · When “Electrode
Cond. Unknown”, “pH x Default Calibration” or “pH x Calibration Expired”
message appears on the LCD, in the Reminder messages area. The “x” represents
channel “1” or channel “2”.
PREPARATION Pour small quantities of the buffer solutions into clean beakers.
If possible, use plastic beakers to minimize any EMC interferences. For
accurate calibration and to minimize cross-contamination, use two beakers for
each buffer solution. One for rinsing the electrode and one for calibration.
45
pH CALIBRATION
If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer
and pH 4.01, 3.00 or 1.68 as second buffer. If you are measuring in the
alkaline range, use pH 7.01 or 6.86 as first buffer and pH 10.01/9.18 or 12.45
as second buffer. For extended range measurements (acidic and alkaline),
perform a five points calibration by selecting five of the available buffers.
CALIBRATION PROCEDURE There are 8 standard pH buffers that are temperature-
compensated during pH calibrations: pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18,
10.01 and 12.45. The standard pH buffers are temperature compensated during
calibration. Custom buffers require the user to use the actual buffer value at
the temperature of use. A minimum of a two point calibration using the
standard pH buffers is required to determine the pH electrode condition. The
buffers should bracket the sample measurement pH. An extended pH measurement
range will require calibration at multiple points. The meter is able of
calibration with 5 pH buffers. For improved measurement accuracy, perform a
multiple buffer calibration bracketing and including the pH range the sample
measurements. The buffer group that will be available during calibration was
set in pH setup Calibration Buffer Entry Type. The following example
demonstrates pH electrode calibration if Manual selection was selected. In
this case all of the 8 standard buffers will be available for calibration.
pH Calibration screen description
· Press
. If the instrument was calibrated before and calibration was not cleared, the old
calibration can be cleared by pressing
. After 10 seconds,
will no longer be
available.
Note: It is very important to clear calibration history when a new electrode is used because
most errors and warning messages that appear during calibration depend on calibration
history.
46
pH CALIBRATION
· Immerse the pH electrode and the temperature probe approximately 4 cm (1.5″) into a buffer
solution of your choice (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 or a custom
buffer) and stir gently. The temperature probe should be close to the pH electrode.
· Select the pH calibration buffer used with
or
. The “Please
wait…” message will appear on the LCD until the reading is stable or the
buffer is validated.
· If the pH buffer is validated,
will appear on the LCD. Press
to update calibration. The calibration buffer will be added to the Calibrated
Buffers section.
· Immerse the pH electrode and the temperature probe into the next buffer solution and follow the
above procedure or press
to exit calibration.
Notes: The new added calibration point will replace
an old one if the difference between them is
±0.2 pH.
If the existing stored calibration is full (five calibration
points), a pop-up menu will be displayed on the LCD
in which you can select with
or
keys the buffer you want to replace with the current
buffer. Press
to delete the selected buffer
and then press
to update calibration with
the new buffer.
· If using manual temperature, after selecting the standard buffer, press
will be displayed on the LCD in which the temperature
value can be adjusted using
or
.
Press
to save the new temperature value.
· If using Custom buffers, press
after buffer
has been accepted to change actual buffer conditions.
A pop-up menu will be displayed on the LCD in which
the custom buffer and the temperature value (MTC)
can be adjusted by pressing
and then
or
keys. Press
to save
the modified value and then
/
to
select next/previous value to be adjusted.
. A pop-up menu
47
pH CALIBRATION
· If the Automatic buffer entry type has been selected for the calibration procedure, the instrument will automatically select the closest buffer to the measured pH value from the edit buffer group (see pH Setup for details).
· If the Semiautomatic buffer entry type has been selected for the calibration procedure, the
instrument will display only the closest buffers to the measured pH value from all the available
buffers and the user must select with
or
the buffer being used.
CALIBRATION MESSAGES
· Move sensor to next buffer or check buffer: this message appears when the difference between the pH reading and the value of the selected calibration buffer is significant. If this message is displayed, check if you have selected the appropriate calibration buffer.
· Wrong buffer temperature: this message appears if the buffer temperature is out of the defined buffer temperature range.
· Clean the electrode or check the buffer. Press
to update calibration: this mes-
sage alerts the user that some dirt or deposits could be on the electrode. Refer to the electrode
Cleaning Procedure.
· Slope too low. Please check the buffer / Slope too high. Please check the buffer: these messages appear if the current slope is under 80 % or over 110 % of default slope. Recalibrate the instrument using fresh buffers.
· Slope too low. Press
to clear old calibration / Slope too high. Press
to
clear old calibration: verify the correct buffer has been selected and poured.
· Unrecognized buffer. Please check the buffer or the buffer list (for Semiautomatic and Automatic buffer entry type): this message appears if the current buffer value is not close to any of the buffers from the buffer list/group. Check if the current buffer is present in the buffer list or the appropriate buffer group was selected.
· The current buffer was already calibrated: change the buffer or press
to exit
calibration mode.
48
pH MEASUREMENT
Verify the pH electrode and instrument has been calibrated before making pH measurements.
DIRECT MEASUREMENT
To measure the pH of a sample using the Direct reading
mode:
· Press
and then
to select pH Mea-
sure mode (select channel first for HI5222).
· Select the Direct reading mode (see pH Setup for
details).
· Place the electrode tip and the temperature probe
approximately 4 cm (1.5″) into the sample to be
tested. Allow time for the electrode to stabilize.
“Stable” will appear.
· The measured pH value will be displayed on the LCD,
together with a short GLP information and display preferences.
Note: If the reading is out of range, “—-” will be displayed on the LCD.
DIRECT/AUTOHOLD MEASUREMENT
To measure pH of a sample using the Direct/AutoHold
reading mode:
· Press
and then
to select pH Mea-
sure mode (for the selected channel – HI5222 only).
· Select the Direct/AutoHold reading mode (see pH
Setup for details).
· Place the electrode tip and the temperature probe approximately 4 cm (1.5″) into the sample to be tested.
· The measured pH value will be displayed on the LCD.
Press
and the “AutoHold” indicator will
start blinking on the LCD until the stability criteria is
reached. The pH value will be frozen on the LCD, along with “AutoHold” indicator.
· To return to normal Measure mode press
.
Note: If the reading is out of range, “—-” will be displayed on the LCD.
49
pH MEASUREMENT
Outside Cal Range warns the user if the current reading is out of the calibrated area. The calibrated area is that part of the pH range in which the calibration point ensures an accurate reading. If the reading is taken out of the calibration area, the “Outside Cal Range”message will start blinking on the LCD. The calibrated area is calculated in accordance with the pH resolution used during the measurement. To avoid triggering this message, the buffer values have to be well-distributed in the desired measurement range.
If measurements are taken successively in different samples, it is recommended to rinse the electrode thoroughly with deionized water or tap water and then with some of the next sample before immersing it into the next sample solution.
The pH reading is affected by temperature. In order to measure the pH accurately, the temperature effect must be compensated. To use the Automatic Temperature Compensation (ATC) feature, connect and place the HI7662-W temperature probe into the sample as close as possible to the electrode and wait for a few seconds.
If the temperature of the sample is known, Manual Temperature Compensation (MTC) can be used by disconnecting the temperature probe.
Notes: For single channel pH measurements (HI5221), “MTC” or “ATC” indicators will be displayed on the LCD, while for dual channel pH measurements (HI5222) “MTC1″/”MTC2” or “ATC1″/”ATC2” indicators will be displayed (Channel dependent).
For mV/Rel mV measurements, “NoProbe” or “TEMP” indicators will be displayed on the LCD (HI5221 only), respectively, “NoProbe 1″/”NoProbe 2” or “TEMP1″/”TEMP2” indicators (HI5222 only) for mV/Rel mV/ISE measurements, depending on the temperature probe status and channel selected.
When in MTC mode, the temperature can be modified by pressing
for pH Measure
mode and
for mV /Rel mV Measure mode (HI5221 only) if the Reading Mode option
is Direct (HI5222 only). The temperature value can be adjusted with
or
from
-20.0 °C to 120.0 °C. Press
to save the new temperature value or press
to return to Measure mode.
When in ATC mode for pH, or TEMP for mV/Rel mV, “—-” will be displayed on the LCD if the measured temperature is under or over temperature range (-20.0 °C to 120.0 °C).
50
mV & Relative mV MEASUREMENTS
mV/ORP MEASUREMENTS
Oxidation-reduction potential (ORP) measurements provide the quantification of
the oxidizing or reducing power of the tested sample. To correctly perform a
redox measurement, the surface of the ORP electrode must be clean and smooth.
DIRECT MEASUREMENT
To measure the mV of a sample using the Direct reading mode:
· Press
and then
to enter mV Measure
mode (Select channel first for – HI5222 only).
· Select the Direct reading mode (see mV Setup for details).
· Place the tip of the ORP electrode 4 cm (1.5″) into the
sample to be tested and allow a few seconds for the
reading to stabilize.
· The instrument will display the measured mV value on
the LCD.
Note: If the reading is out of range, “—-” will be displayed on the LCD.
DIRECT/AUTOHOLD MEASUREMENT
To measure mV of a sample using the Direct/AutoHold
reading mode:
· Press
and then
to select mV Measure
mode (Select channel first for – HI5222 only).
· Select the Direct/AutoHold reading mode (see mV Setup
for details).
· Place the tip of the ORP electrode approximately 4 cm
(1.5″) into the sample to be tested.
· The measured mV value will be displayed on the LCD.
Press
and the “AutoHold” indicator will start
blinking on the LCD until the stability criteria is reached. The mV value will be frozen on the
LCD, along with “AutoHold” indicator.
· To return to normal Measure mode press
.
Note: If the reading is out of range, “—-” will be displayed on the LCD.
51
mV & Relative mV MEASUREMENTS
Relative mV MEASUREMENTS
To measure the Relative mV of a sample:
· Press
then
HI5222).
(select channel first for
· Verify if a current calibration has been made.
· If required, conduct the single point Rel mV calibration. Verify the tip of the electrode is immersed into the known solution or ORP standard.
· Press
. Use
and
keys
set the standard value. Press
to store the
calibration.
· Press
then
HI5222).
(select channel first for
· Place calibrated sensor tip into the sample to be analyzed. The instrument will display the measured Relative mV value on the LCD, together with a short GLP information about the last calibration or Offset: 0.0 mV no Rel mV calibration was performed.
Notes: If the ORP sensor is not in solution or the measured mV potential is out of range, “—-” will be displayed on the LCD.
52
ISE CALIBRATION (HI5222 only)
For greater accuracy, it is recommended to calibrate the ISE sensors
frequently. The instrument should be recalibrated when “ISE x Calibration
Expired” (the “x” represents channel “1” or channel “2”) message appears on
the LCD, in the Reminder messages area. Due to electrode conditioning time,
the electrode must be kept immersed a few seconds to stabilize. The user will
be guided step by step during calibration with easy-to-follow messages on the
display. This will make the calibration a simple and error-free procedure.
PREPARATION Pour small quantities of the standard solutions into clean
beakers. If possible, use plastic beakers to minimize any EMC interferences.
For accurate calibration and to minimize cross-contamination, use two beakers
for each standard solution. One for rinsing the electrode and one for
calibration.
Note: To read concentration (not activity) ISA must be added to the standards
and samples. No corrections are needed due to dilutions. CALIBRATION PROCEDURE
The ISE calibration and measurement can be performed with or without
temperature compensation. If the temperature compensation option is enabled,
the isopotential point of the electrode must be set in ISE Setup in order to
perform correct concentration measurements. Before calibrating, make sure that
the appropriate Electrode Type has been selected in ISE Setup according to the
measured Ion/compound. ISE Calibration screen description
53
ISE CALIBRATION (HI5222 only)
2 cm (1″)
The group of calibration standards is set under ISE Setup Calibration. Select standards that are in the measurement range of the samples.
To calibrate the instrument:
· Press
. If the instrument was calibrated before and calibration was not cleared, the old
calibration can be cleared by pressing
. After 10 seconds,
will no longer be
available.
Note: It is very important to clear calibration history when a new electrode is used because most errors and warning messages that appear during calibration depend on calibration history.
· Add ISA to both standard solutions and samples.
· Immerse the Ion Selective Electrode and the temperature probe approximately 2 cm (1″) into the less concentrated standard solution and stir gently.
· Select the appropriate standard solution concentration with
or
. For All Standards manual entry mode, the standard
concentration can be selected from a list containing all the predefined and
custom standards. For Group Standard manual entry mode the standard
concentration can be selected from the predefined group of standards.
Press
to calibrate the electrode in the standard.
Note: To adjust standard value: Press
. A pop-up menu will be displayed on the LCD
in which the concentration value can be adjusted using
or
. Press
to save the new concentration value.
· The “Please wait…” message will appear on the LCD for 10 seconds. Remove ISE from first
standard, rinse tip and immerse the Ion selective electrode and the temperature probe into the
next standard solution and follow the above procedure or press
to exit calibration.
Notes: The new added calibration point will replace
an old one if the difference between them is less than
20 % of the standard solution.
If the existing stored calibration is full (five calibration
points), a pop-up menu will be displayed on the LCD
in which you can select with
or
the standard solution you want to replace with the
current one.
54
ISE CALIBRATION (HI5222 only)
Press
to delete the selected calibrated point and then press
calibration with the new standard solution.
If the isopotential point of the electrode is unknown, the ISE calibration and measurements can be performed without temperature compensation (see ISE Setup, Temperature option for details).
When in MTC mode, after selecting a standard press
, a pop-up menu will be displayed on the LCD in which the concentration and the temperature
value can be adjusted by pressing
and then
or
keys. Press
to save
the modified value and then
/
to select next/previous value to be adjusted. MTC
value will have no effect on measurement but will
be included on log data.
to update
CALIBRATION MESSAGES
· Wrong standard solution. Check the standard solution: this message appears when the difference between the reading and the value of the selected standard solution concentration is significant. If this message is displayed, check if you have selected the appropriate calibration standard.
· Standard to close. Check the standard or clear calibration: this message appears when the difference between current ISE standard and the already calibrated standard is too low.
· Slope to low/high. Check the standard solution. Recalibrate using fresh standards.
· Difference between standards temperature is too high. Press or clear old calibration.
to update calibration
55
ISE MEASUREMENT (HI5222 only)
Make sure the instrument and ISE sensor have been calibrated before making ISE
measurements.
When using one of the incremental methods for measurement, at least a two
point ISE calibration must be performed to establish the electrode slope. For
accurate measurements, add the appropriate ISA (Ionic Strength Adjuster) to
both samples and standards. Consult ISE manual for sensor preparation details.
DIRECT MEASUREMENT
To measure the concentration of a sample using the Direct reading mode:
· Press
and then
to select ISE Measure
mode for the selected channel.
· Select the Direct reading mode (see ISE Setup for details).
· Add ISA to the sample solution.
· Submerge the Ion Selective Electrode tip and the temperature probe approximately 2 cm (1″) into the sample. Allow time for the electrode to stabilize.
· The measured concentration value will be displayed on the LCD in the selected units.
Note: If the reading is out of range, “—-” will be displayed on the LCD.
DIRECT/AUTOHOLD MEASUREMENT
To measure the concentration of a sample using the
Direct/AutoHold reading mode:
· Press
and then
to select ISE Measure
mode for the selected channel.
· Select the Direct/AutoHold reading mode (see ISE Setup
for details).
· Add ISA to the sample solution.
· Dip the Ion Selective Electrode tip and the temperature
probe approximately 2 cm (1″) into the sample to be
tested.
· The measured concentration value will be displayed on the LCD. Press
, the “AutoHold”
indicator will blink on the LCD until the stability criterion is reached. The concentration value will
be frozen on the LCD, along with “AutoHold” indicator.
56
ISE MEASUREMENT (HI5222 only)
· To return to normal Measure mode press
.
Note: If the reading is out of range, “—-” will be displayed on the LCD.
KNOWN ADDITION
To measure the concentration of a sample using the Known Addition incremental method:
· Press
and then
to select ISE
Measure mode for the selected channel.
· Select the Known Addition method (see ISE Setup for details).
· Prior to starting a KA procedure, the ISE sensor must be calibrated with a minimum of two standards containing ISA. The slope of the electrode will be used in all calculations involved in KA.
· If following an established procedure: Press
,
Edit the method variables and follow the procedure
below.
· Press
to set the method parameters. Press
/
to select next/previous parameter
to edit, then press
and use
or
to set the desired parameter value. Press
to save the modified value and then press
to exit method parameters edit menu.
· If developing a procedure: Before attempting Known Addition analysis it is important to determine what sample volume, standard concentration and standard volume will produce the best results. As a general rule, the addition of standard should change the mV value of the sample by 15 – 20 mV. For a positively charged ion (i.e. Sodium, Potassium, Calcium), the standard addition should increase the mV. For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the standard addition should decrease the mV. Start with a small trial. For example: Measure 50 mL of sample, add a magnetic stir bar, place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the highest ISE standard available (i.e. 0.1M or 1000 ppm).
57
ISE MEASUREMENT (HI5222 only)
Start by adding 500 µL at a time (for example). Watch the change in mV. When you have
observed approximately a 15 mV change from the original sample, calculate the total volume
added. Adjust sample and standard volumes proportionally to standard volumes that can be
measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.
· Press
: Edit the procedure variables to the volumes determined in the prior step.
Procedure:
· Press
to enter Known Addition mode.
· Volumetrically add sample to a clean beaker. Add a magnetic stir bar and place on a stir plate.
Stir sample. The method will prompt user to add ISA. Place ISE sensor tip into the solution and
a mV value will show on the display.
· Press
to take the first mV reading.
· When the reading is stable, press
to store the first mV reading. The second step of
the method will be displayed on the LCD in which the user is notified to add the Volume of
Standard to the sample.
· Press
to take the second mV reading.
· When the reading is stable, press
to store
the second mV reading. The ISE measurement results
will be displayed on the LCD.
· Press
to log the current results into a ISE
Method Report. Press
to return to ISE
Measure mode. Press
to measure additional
samples. Rinse ISE sample between samples.
· Press
to modify the method parameters.
Note: Press
at any time to stop the
measurement and return to ISE Measure mode.
KNOWN SUBTRACTION
To measure the concentration of a sample using the Known Subtraction method:
· Press
and then
to select ISE Measure mode for the selected channel.
· Select the Known Subtraction method (see ISE Setup Reading Mode).
· Prior to starting a KS procedure, the ISE sensor must be calibrated with a minimum of two
standards containing ISA. The slope of the electrode will be used in all calculations involved in
KS.
58
ISE MEASUREMENT (HI5222 only)
· If following an established procedure: Press
then edit the method variables and follow
the procedure below.
· Press
to set the method parameters. Press
/
to select next/previ-
ous parameter to edit, then press
and use
or
to set the desired
parameter value. Press
to save the modified value and then press
to exit
method parameters edit menu.
· If developing a procedure: Before attempting Known Subtraction analysis it is important to
determine what sample volume, standard reactant concentration and standard volume will
produce the best results and the way the reagent will react with the measured ion on a molar
basis (stoichiometric factor). As a general rule, the addition of standard should change the mV
value of the sample by 15-20 mV.
For a positively charged ion (i.e. Calcium), the reactant addition should decrease the mV. For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the reactant addition should increase the mV. Start with a small trial. For example: Measure 50 mL of sample, add a magnetic stir bar, place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip in to the sample. Put instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the reactant standard. Start by adding 500 µL at a time (for example). Watch the change in mV. When you have observed approximately a 15 mV change from the original sample, calculate the total volume added. Adjust sample and standard volumes proportionally to standard volumes that can be measured with accuracy. Use volumetric pipettes for standard, ISA and reagent addition.
· Press
then edit the procedure variables to the volumes determined in the prior step.
Procedure:
· Press
to enter Known Subtraction mode.
· Volumetrically add sample to a clean beaker. Add a magnetic stir bar and place on a stir plate. Stir sample. The method will prompt user to add ISA. Place ISE sensor tip into the solution and a mV value will show on the display.
· Press
to take the first mV reading.
· When the reading is stable, press
to store the first mV reading. The second step of the
method will be displayed on the LCD in which the user is notified to add the volume of reagent
to the sample.
· Press
to take the second mV reading.
59
ISE MEASUREMENT (HI5222 only)
· When the reading is stable, press
to store the second mV reading. The ISE measurement
results will be displayed on the LCD.
· Press
to log the current results into an ISE Method Report. Press
to return to
ISE Measure mode. Press
to start another measurement. Rinse ISE sensor between
samples.
· Press
, to modify parameters.
Note: Press
at any time to stop the measurement and return to ISE Measure mode.
ANALYTE ADDITION
To measure the concentration of a sample using Analyte Addition method:
· Press
and then
to select ISE Measure mode for the selected channel.
· Select the Analyte Addition method (see ISE Setup Reading Mode).
· Prior to starting an AA procedure, the ISE sensor must be calibrated with a minimum of two
standards containing ISA. The slope of the electrode will be used in all calculations involved in
AA.
· If following an established procedure: Press
then edit the method variables and follow
the procedure below.
· Press
to set the method parameters. Press
/
to select next/previ-
ous parameter to edit, then press
and use
or
to set the desired
parameter value. Press
to save the modified value and then press
to exit
method parameters edit menu.
· If developing a procedure: Before attempting Analyte Addition analysis, it is important to
determine which standard volume, concentration and sample size will produce the best results.
As a general rule, the standard must be less concentrated than the sample so the addition of
sample will increase the total ion content of the solution from the beaker and change the mV
value by at least 10 mV. For a positively charged ion (i.e. Sodium), the AA increases the mV.
For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the AA should decrease the mV.
Start with a small trial. For example: Measure 50 mL of standard, add a magnetic stir bar and
place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put
instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the
sample. Start by adding 500 µL at a time (for example). Watch the change in mV. When you
have observed approximately a 10 mV change from the original standard, calculate the total
volume added. Adjust sample and standard volumes proportionally to standard volumes that
can be measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.
60
ISE MEASUREMENT (HI5222 only)
· Press
then edit the procedure variables to the volumes determined in the prior step.
Procedure:
· Press
to enter Analyte Addition mode.
· Volumetrically add standard to a clean beaker. Add a magnetic stir bar and place on a stir plate.
Stir standard. The method will prompt user to add ISA. Place ISE sensor tip into the solution
and a mV value will show on the display.
· Press
to take the first mV reading.
· When the reading is stable, press
to store the first mV reading. The second step of the
method will be displayed on the LCD, in which the user is notified to add the Sample Volume
to the standard solution. The method parameters are also displayed on the LCD.
· Press
to take the second mV reading.
· When the reading is stable, press
to store the second mV reading. The ISE measurement
results will be displayed on the LCD.
· Press
to log the current results into an ISE Method Report. Press
to return to
ISE Measure mode.
· Press
to start another measurement. Rinse ISE sensor between samples.
· Press
, to modify the method parameters.
Note: Press
at any time to stop the measurement and return to ISE Measure mode.
ANALYTE SUBTRACTION
To measure the concentration of a sample using Analyte Subtraction method:
· Press
and then
to select ISE Measure mode for the selected channel.
· Select the Analyte Subtraction method (see ISE Setup Reading Mode).
· Prior to starting an AS procedure, the ISE sensor must be calibrated with a minimum of two standards containing ISA. The slope of the electrode will be used in all calculations involved in AS.
· If following an established procedure: Press the procedure below.
then edit the method variables and follow
· Press
to set the method parameters. Press
/
to select next/previ-
ous parameter to edit, then press
and use
or
to set the desired
parameter value. Press
to save the modified value and then press
to exit
method parameters edit menu.
61
ISE MEASUREMENT (HI5222 only)
· If developing a procedure: Before attempting Analyte Subtraction analysis, it is important to
determine which sample volume, reactant volume and concentration, will produce the best results
and the way the reagent will react with the measured ion on a molar basis (stoichiometric fac-
tor). As a general rule, the reactant should contain the measured ion so the sample addition
will react with the ion and reduce the measured concentration of the sample. The change of the
mV value, before and after the sample addition, should be at least 10 mV. Start with a small
trial. For example: Measure 50 mL of reactant, add a magnetic stir bar and place on a stirrer,
add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put instrument in
mV mode and record the observed mV. Using a micropipette, add a volume of the sample.
Start by adding 500 µL at a time (for example). Watch the change in mV. When you have
observed approximately a 10 mV change from the original value, calculate the total volume
added. Adjust sample and standard volumes proportionally to standard volumes that can be
measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.
· Press
then edit the procedure variables to the volumes determined in the prior step.
Procedure:
· Press
to enter Analyte Subtraction mode.
· Volumetrically add reactant to a clean beaker. Add a magnetic stir bar and place on a stir plate. Stir standard. The method will prompt user to add ISA. Place ISE sensor tip into the solution and a mV value will show on the display.
· Press
to take the first mV reading.
· When the reading is stable, press
to store the first mV reading. The second step of the
method will be displayed on the LCD in which the user is notified to add the Sample Volume
to the standard solution.
· Press
to take the second mV reading.
· When the reading is stable, press
to store the second mV reading. The ISE measurement
results will be displayed on the LCD.
· Press
to log the current results into an ISE Method Report. Press
to return
to ISE Measure mode. Press
to start another measurement. Rinse sensor between
samples.
· Press
to modify the method parameters.
Note: Press
at any time to stop the measurement and return to ISE Measure mode.
62
TEMPERATURE CALIBRATION
The user temperature calibration menu can be accessed during meter startup by simultaneously pressing three keys as shown in the drawing below. Press the keys after the short beep is heard at the meter power on. Keep all three keys pressed until Temp. Calibration menu appears.
Note: The user temperature calibration is performed at three points: around 0 °C, 50 °C and 100 °C.
To perform the user temperature calibration:
· Select the desired temperature channel by pressing
(HI5222 only).
· Press
to start the temperature calibration.
Adjust the temperature preset value using
or
when necessary.
· Insert the temperature probe into the beaker with water
at 0 °C.
· Wait for measurement to stabilize and then press
to confirm the calibration point.
· Repeat the previous steps for 50 °C and 100 °C.
· Save the calibration.
· Press
to return to measure mode.
Note: Press
if you want to clear the temperature user calibration.
63
LOGGING
There are 5 ways the Reading Mode and Log may be configured together. The table below shows the combinations and indicates where the completed log will be stored.
Reading Mode
Log Automatic (1)
Direct
Manual (2)
Auto Hold (NA)
Direct/AutoHold
Automatic (3) Manual (4) Auto Hold (5)
log Recall Automatic Log Manual Log Not Applicable Automatic Log Manual Log Manual Log
- Direct Reading Mode and Automatic Log:
Real time continuous measurements are on display with
continuous logging to meter memory. Press
.
64
LOGGING
- Direct Reading Mode and Manual Log:
Real time continuous measurements are on display and
snapshots of measurement data are stored in the Manual
log when the user presses
. Subsequent snapshots
will be added to the same Manual Lot every time the
is depressed unless New Lot is selected under
Log options.
Note: When the
is pressed the lot ID along
with the current record number will appear for
short time on the selected channel window on the
top/left corner (e.g. L033_MV 8 – this means lot
ID L033_mV and record number 8).
- Direct/AutoHold Reading Mode and Automatic Log
Press
and then
keys must be pressed
on front display to initiate this function. Real time continuous
measurements are on display with “AutoHold” flashing
and real time continuous logging into meter memory,
until the meter reaches the stability criteria to go into
AutoHold mode. The stored sample logs will be marked
with an “H” to indicate the AutoHold mode. The virtual
key
returns operation to real time continuous
measurements and
stops the logging session.
- Direct/AutoHold Reading Mode and Manual Log
Press
in order to add one new record in the log report. The manual log is working even
if it is in AutoHold or Continuous Reading mode. Press
to initiate the AutoHold event.
“AutoHold” will flash until the stability criteria is reached and then the screen freezes in Auto
Hold mode, the data is marked with an “H”.
65
LOGGING
- Direct/AutoHold Reading Mode and Auto Hold Log
Press
and then
keys initiate and automate the capture of stable data which is
stored in the Recall Manual Log file. During the process, “AutoHold” will flash until the stability
criteria is reached and then the screen freezes in AutoHold mode, the data is logged and marked
with an “H”. The virtual key
returns operation to Real time continuous measurement.
Press
again to log a second stable data point. The lot ID along with the record index will
appear for short time on the top/left corner on the selected channel window, every time a record
will be added to the lot.
LOG RECALL
This feature allows the user to view all stored data. If
no data was logged, the “No records were found”
message will be displayed on the LCD in the Log Recall
screen. Otherwise, the instrument will display all the
memorized lots in accordance with the selected option:
Automatic Log, Manual Log or ISE Method Report
(HI5222 only).
To view the memorized data:
· Press
while in Measure mode.
· Press
. The “Choose Log Report Type”
message will be displayed in the Reminder
messages area.
· Press
,
or
to select the
desired Log Report type. All logged lots for the selected
Log Report type will be displayed on the LCD.
· To filter the displayed lots, press
and then
the desired parameter
,
or
-HI5222 only. Only the selected measurement
parameter lots will be displayed on the LCD.
66
LOGGING
· Select the desired lot with
or
and
press
to display the logged data from the
highlighted lot. The “Please wait…” message will
be displayed on the LCD for one second. The selected
Logging Data Configuration options will be displayed
on the LCD, together with GLP information (last
calibration date and calibrated buffers/standards)
if a calibration has been performed on the selected
mode and the logged values (measured value, mV
value, temperature value, temperature compensation
mode and the logging time).
Note: For automatic logging only, it is possible to view the plotted graph.
Press
to display the graph.
By pressing
it is possible to move the
graph along the X or Y axis with the arrow keys.If
pressing
while the graph is displayed, the
zoom menu for the X and Y axes will be accessed.
Press
or
/
/
/
to switch between the
active zooming axes and then zoom in or out
on the selected axis by pressing the appropriate
virtual key.
· Press
to return to the previous menu at
any time.
67
LOGGING
To delete lots:
· Press
while in Log Recall mode.
· Press
or
to access delete or delete
all mode. Otherwise, press
to return to Log
Recall view mode.
· After selecting one of the deleting modes, use
or
to select one lot and then press
or
to delete the selected lot or all lots. The
“Please wait…” message will be displayed on the
LCD until the selected lot or all lots are deleted.
· Press
and then press
to exit deleting
mode and return to Log Recall view mode.
· Press
to exit Log Recall mode and return
to Measure mode.
Note: Logged lots should also be deleted whenever “Please Delete Old Log Files” or “Low Data
Logging Space” message appears on the LCD, in the Reminder messages area.
Data transmission from the instrument to the PC can be done with the HI92000
Windows® compatible software (optional). HI92000 also offers graphing and on-
line help features. Data logged on the HI5221 and HI5222 meters can be
exported to the most popular spreadsheet programs for further analysis. HI5221
and HI5222 instruments have an USB interface available.
Use a standard USB cable to connect your instrument to the PC. Make sure that
the instrument and the HI92000 software have the same baud rate and the
appropriate communication port. The PC software may also be used for real time
logging. To allow our users access to the latest version of Hanna Instruments
PC compatible software, we made the products available for download at
http://software.hannainst.com. Select the product code and click Download Now.
After download is complete, use the setup.exe file to install the software.
PC INTERFACE
68
ADDITIONAL INFORMATION
ISE THEORY
An Ion Selective Electrode (ISE) is an electrochemical sensor that changes
voltage with the activity or concentration of ions in solutions. The change in
voltage is a logarithmic relationship with concentration, and is expressed by
the Nernst equation:
E = Eo + S log(a)
where: E – the measured voltage; Eo – standard voltage and other standard system voltages; a – the activity of the ion being measured;
S
=
2.303RT nF
S – the Nernst slope factor and is derived from thermodynamic principles: R – the universal gas constant (8.314 J/(Kmol)); T – the temperature in degrees Kelvin; F – the Faraday’s constant (96,485 C/mol); n – the ion charge. The slope may be positive or negative depending upon the Ion charge (n).
SPECIES
Monovalent cation Monovalent anion Divalent cation Divalent anion
SLOPE (mV/decade)
+59.16 59.16 +29.58 29.58
Activity and concentration are related by an “activity coefficient”, expressed
as:
a = · C
where: a – the activity of the ion being measured; – the activity coefficient;
C – the concentration of the ion being measured.
69
ADDITIONAL INFORMATION
In very dilute solutions approaches 1 so activity and concentration are the
same. Actual samples that are more concentrated have much smaller activity
coefficients ( < 1). The addition of an inert background salt to standards and
samples stabilizes the activity coefficient so that concentration measurements
may be made directly. Some of Hanna Instruments’s Ionic Strength Adjuster
formulations also may optimize pH and complex interferences, in addition to
standardizing the ionic strength.
The Nernst equation can be written: E = Eo + S log(C)
ION SELECTIVE ANALYSIS METHODS
Direct Analysis
This method is a simple procedure for measuring multiple samples. It should
only be used in the linear working regions of the sensor. A direct reading
instrument such as the HI5222 determines concentration of the unknown by a
direct reading after calibrating the instrument with the standards. The
instrument is calibrated as described in “ISE CALIBRATION & MEASUREMENTS”
section, with two or more freshly made standards that are in the measurement
range of the unknowns. Ionic strength adjustment is made to samples and
standards. Unknowns are measured directly by the instrument. At lower
concentrations, in non-linear regions of the electrode response, multiple
calibration points will extend measurements to a practical detection limit.
Calibrations must be performed more frequently in these cases.
Incremental Methods
Incremental methods are useful for the measurement of samples whose constituents are variable or concentrated. Incremental techniques can reduce errors from such variables as temperature, viscosity, or pH extremes and will provide indirect analysis of ions for which there is no ISE sensor for a direct measurement. There are four commonly used different incremental methods for sample measurement. They are Known Addition, Known Subtraction, Analyte Addition and Analyte Subtraction. HI5222 allows the analyst to use these techniques as a simple routine procedure, thus eliminating calculations or tables. The method once set up can be used for repetitive measurements on multiple samples. Known Addition and Known Subtraction
With Known addition, standard is added to a sample being measured. The standard and sample contain the same ion. mV are taken before and after the standard addition. From the change in mV, the sample concentration is determined.
C ·V
(V + V )
CSAMP = (V
STD STD
E
+ V + V )·10 S- (V
· SAMP
ISA
+V ) V
SAMP
STD
ISA
SAMP
ISA
SAMP
70
ADDITIONAL INFORMATION
With Known subtraction, a known standard is added to an ionic sample being measured. The standard reacts with the measured ion in the sample in a known manner, thus removing measured ions from the solution. From the change in mV, the concentration of the sample is determined.
C ·V ·f
(V + V )
CSAMP
=
(VSAMP
VISA)
–
STD STD
(VSAMP +
VSTD
·E VISA)·10 S
SAMP
ISA
VSAMP
where: CSAMP – the sample concentration; CSTD – the standard concentration; VSAMP – the sample volume;
E – the difference of potential from the electrode;
S – the electrode slope, determined in a previous calibration;
VSTD – the standard volume;
f – the stoichiometric ratio between sample
VISA – ISA volume
and standard;
Example 1
You have sulfide samples and you are adding Ag+. The reaction is:
S2- + 2Ag+ Ag2S One mole sulfide sample reacts with 2 moles silver standard (f = 1/2).
Example 2
You have sulfide samples and you are adding Pb2+. The reaction is:
S2- + Pb2+ PbS One mole sulfide sample reacts with 1 mole lead standard (f = 1).
Analyte Addition and Analyte Subtraction
Analyte Addition and Subtraction are variations of the previous two methods.
With Analyte Addition, sample (analyte) is added to an ion standard being measured. The
standard and sample contain the same ion. mV are taken before and after the sample addition.
From the mV the analyte concentration is determined.
E
C · V (V + V + V )·10
CSAMP
=
STD
(VSTD
VSTIDSA·)
STD
SAMP
ISA
VSAMP
–
(SVSTD
V) ISA
With Analyte Subtraction, sample (analyte) is added to an ion standard being measured. The
analyte reacts with the measured ion in a known manner thus removing measured ions from the
solution. From the change in mV the concentration of the analyte is determined.
{ [ ] } ( ) C =f · SAMP
(VSTD + VISA) V
1 + (VSTD + VISA)
E
·10 S
V
· CSTD·VSTD V +V
SAMP
SAMP
STD
ISA
71
where: CSAMP – the sample concentration; E- the difference of potential from the electrode;
C STD
– the standard concentration; S
– the electrode slope, determined in a previous
VSAMP – the sample volume; VSTD – the standard volume; V – ISA volume;
calibration;
f – the stoichiometric ratio between sample and standard;
ISA
pH BUFFER TEMPERATURE DEPENDENCE
Temperature has an effect on pH. The calibration buffer solutions are affected
by temperature changes to a lower degree than normal solutions.
During calibration the instrument will automatically calibrate to the pH value
corresponding to the measured or set temperature.
TEMP
pH BUFFERS
°C
K °F 1.679 3.000 4.010 6.862 7.010 9.177 10.010 12.454
0 273 32 1.670 3.072 4.007 6.982 7.130 9.459 10.316 13.379
5 278 41 1.670 3.051 4.002 6.949 7.098 9.391 10.245 13.178
10 283 50 1.671 3.033 4.000 6.921 7.070 9.328 10.180 12.985
15 288 59 1.673 3.019 4.001 6.897 7.046 9.273 10.118 12.799
20 293 68 1.675 3.008 4.004 6.878 7.027 9.222 10.062 12.621
25 298 77 1.679 3.000 4.010 6.862 7.010 9.177 10.010 12.450
30 303 86 1.683 2.995 4.017 6.851 6.998 9.137 9.962 12.286
35 308 95 1.688 2.991 4.026 6.842 6.989 9.108 9.919 12.128
40 313 104 1.693 2.990 4.037 6.837 6.983 9.069 9.881 11.978
45 318 113 1.700 2.990 4.049 6.834 6.979 9.040 9.847 11.834
50 323 122 1.707 2.991 4.062 6.834 6.978 9.014 9.817 11.697
55 328 131 1.715 2.993 4.076 6.836 6.979 8.990 9.793 11.566
60 333 140 1.724 2.995 4.091 6.839 6.982 8.969 9.773 11.442
65 338 149 1.734 2.998 4.107 6.844 6.987 8.948 9.757 11.323
70 343 158 1.744 3.000 4.123 6.850 6.993 8.929 9.746 11.211
75 348 167 1.755 3.002 4.139 6.857 7.001 8.910 9.740 11.104
80 353 176 1.767 3.003 4.156 6.865 7.010 8.891 9.738 11.003
85 358 185 1.780 3.002 4.172 6.873 7.019 8.871 9.740 10.908
90 363 194 1.793 3.000 4.187 6.880 7.029 8.851 9.748 10.819
95 368 203 1.807 2.996 4.202 6.888 7.040 8.829 9.759 10.734
During calibration the instrument will display the pH buffer value at 25 °C.
72
ELECTRODE CONDITIONING AND MAINTENANCE
PREPARATION PROCEDURE Remove the protective cap off the pH electrode. SALT
DEPOSITS MAY BE PRESENT. This is normal with electrodes. They will disappear
when rinsed with water. During transport, tiny bubbles of air may form inside
the glass bulb affecting proper functioning of the electrode. These bubbles
can be removed by “shaking down” the electrode as you would do with a glass
thermometer. If the bulb and/or junction is dry, soak the electrode in HI70300
or HI80300 Storage Solution for at least one hour.
For refillable electrodes: If the filling solution (electrolyte) is more than
2 cm (1″) below the fill hole, add HI7082 or HI8082 3.5M KCl Electrolyte
Solution for double junction or HI7071 or HI8071 3.5M KCl+AgCl Electrolyte
Solution for single junction electrodes. Unscrew the fill hole screw during
measurements. This will allow electrolyte to flow out of the junction.
73
ELECTRODE CONDITIONING AND MAINTENANCE
For AmpHel® electrodes:
If the electrode does not respond to pH changes, the battery may have run down
and the electrode or battery (if replaceable) should be replaced.
MEASURE Rinse the pH electrode tip with distilled water. Immerse the tip
bottom 4 cm (1.5″) in the sample and stir gently for a few seconds. For a
faster response and to avoid cross-contamination of the samples, rinse the
electrode tip with a few drops of the solution to be tested, before taking
measurements.
STORAGE PROCEDURE To minimize clogging and ensure a quick response time, the
glass bulb and the junction of pH electrode should be kept moist and not
allowed to dry out. Replace the solution in the protective cap with a few
drops of HI70300 or HI80300 Storage Solution or, in its absence, Filling
Solution (HI7071 or HI8071 for single junction and HI7082 or HI8082 for double
junction electrodes). Follow the Preparation Procedure before taking
measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE Inspect the electrode and the cable. The cable used for
connection to the instrument must be intact and there must be no points of
broken insulation on the cable or cracks on the electrode stem or bulb.
Connectors must be perfectly clean and dry. If any scratches or cracks are
present, replace the electrode. Rinse off any salt deposits with water.
pH PROBE MAINTENANCE
For refillable electrodes: Refill the reference chamber with fresh electrolyte
(HI7071 or HI8071 for single junction or HI7082 or HI8082 for double junction
electrodes). Allow the electrode to stand upright for 1 hour. Follow the
Storage Procedure above.
pH CLEANING PROCEDURE
· General Soak in Hanna Instruments HI7061 or HI8061 General Cleaning Solution
for approximately 30 minutes.
· Protein Soak in Hanna Instruments HI7073 Protein Cleaning Solution for 15
minutes. · Inorganic Soak in Hanna Instruments HI7074 Inorganic Cleaning
Solution for 15 minutes.
This is good at cleaning a black ceramic junction. · Oil/grease Rinse with
Hanna Instruments HI7077 or HI8077 Oil and Fat Cleaning Solution.
74
IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least 1 hour before taking measurements.
TROUBLESHOOTING GUIDE
SYMPTOMS Slow response/excessive drift
Readings fluctuate up and down (noise).
The LCD displays “_” during measurements (pH, mV, mV Rel or ISE).
Out of range in the mV scale.
The instrument does not work with the temperature probe. The meter fails to
calibrate or gives faulty readings. Explicit warnings are displayed during
calibration. The electrode condition is not displayed after calibration. The
instrument does not start up.
PROBLEM
SOLUTION
Dirty pH electrode. Dirty reference junction.
Soak the electrode tip in HI7061 solution for 30 min. and then clean the electrode. Soak in HI7074.
Clogged/dirty junction. Low electrolyte level (refillable electrodes only).
Out of range in the appropriate scale.
Dry reference junction. Out order temperature probe.
Clean the electrode. Refill with fresh solution (for refillable electrodes
only).
Check to see if the pH bulb and reference junction are in the solution. Make
sure the sample is in the specified range.Check the electrolyte level and the
general state of the pH/ORP or ISE electrode.
Soak in HI70300 Storage solution for at least one hour.
Replace the probe.
Broken or out of order electrode.
Dirty/broken electrode, contaminated buffers.
Only one-point calibration has been performed.
Internal or software error.
Replace the electrode.
Follow displayed instructions.
Perform at least a two-point calibration. Restart the instrument using the
power switch or unplug adapter.If the error persists, contact your local Hanna
Instruments Office.
75
TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS
The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher.
Since the resistance of the pH electrode is in the range of 50 200 M, the
current across the membrane is in the pico Ampere range. Large currents can
disturb the calibration of the electrode for many hours.
The pH electrode’s life also depends on the temperature. If constantly used at
high temperatures, the electrode life is drastically reduced.
Typical Electrode Life Ambient Temperature 90 °C (194 °F) 120 °C (248 °F)
1 3 years Less than 4 months Less than 1 month
Alkaline Error
High concentrations of sodium ions interfere with readings in alkaline
solutions. The pH at which the interference starts to be significant depends
upon the composition of the glass. This interference is called alkaline error
and causes the pH to be underestimated.
76
ACCESSORIES
pH BUFFER SOLUTIONS
HI6016
pH 1.679 Buffer Solution, 500 mL bottle
HI6003
pH 3.000 Buffer Solution, 500 mL bottle
HI8004L
pH 4.01 Buffer Solution in FDA approved bottle, 500 mL
HI6004
pH 4.010 Buffer Solution, 500 mL bottle
HI8006L
pH 6.86 Buffer Solution in FDA approved bottle, 500 mL
HI6068
pH 6.862 Buffer Solution, 500 mL bottle
HI8007L
pH 7.01 Buffer Solution in FDA approved bottle, 500 mL
HI6007
pH 7.010 Buffer Solution, 500 mL bottle
HI8009L
pH 9.18 Buffer Solution in FDA approved bottle, 500 mL
HI8010L
pH 10.01 Buffer Solution in FDA approved bottle, 500 mL
HI6010
pH 10.010 Buffer Solution, 500 mL bottle
HI6124
pH 12.450 Buffer Solution, 500 mL bottle
ELECTRODE STORAGE SOLUTIONS
HI70300L
Storage Solution, 500 mL bottle
HI80300L
Storage Solution in FDA approved bottle, 500 mL
ELECTRODE CLEANING SOLUTIONS
HI70000P
Electrode Rinse Sachets, 20 mL, 25 pcs
HI7061L
General Purpose Solution, 500 mL bottle
HI7073L
Protein Cleaning Solution, 500 mL bottle
HI7074L
Protein Cleaning Solution, 500 mL bottle
HI7077L
Oil & Fat Cleaning Solution, 500 mL bottle
HI8061L
General Purpose Solution in FDA approved bottle, 500 mL
HI8073L
Protein Cleaning Solution in FDA approved bottle, 500 mL
HI8077L
Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL
ELECTRODE REFILL ELECTROLYTE SOLUTIONS
HI7071
3.5M KCl + AgCl Electrolyte, 4×30 mL, for single junction electrodes
HI7072 HI7082
1M KNO3 Electrolyte, 4×30 mL 3.5M KCl Electrolyte, 4×30 mL, for double junction electrodes
77
ACCESSORIES
HI8071
3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4×30 mL, for single junction electrodes
HI8072 HI8082
1M KNO3 Electrolyte in FDA approved bottle, 4×30 mL 3.5M KCl Electrolyte in FDA approved bottle, 4×30 mL, for double junction electrodes
HI8093
1M KCl + AgCl Electrolyte in FDA approved bottle, 4×30 mL
ORP SOLUTIONS
HI7021L
Test Solution 240 mV, 500 mL bottle
HI7022L
Test Solution 470 mV, 500 mL bottle
HI7091L
Reducing Pretreatment Solution
HI7092L
Oxidizing Pretreatment Solution, 500 mL
pH ELECTRODES
All electrodes part numbers ending in B are supplied with a BNC connector and
1 m (3.3′) cable, as shown below:
HI1043B Glass body, double junction, refillable, combination pH electrode. Use: strong acid/alkali.
HI1053B Glass body, triple ceramic, conical shape, refillable, combination pH
electrode. Use: emulsions.
78
ACCESSORIES
HI1083B Glass body, micro, Viscolene, non refillable, combination pH
electrode. Use: biotechnology, micro titration.
HI1131B Glass body, refillable, double junction, combination pH electrode.
Use: general purpose.
HI1330B Glass body, semimicro, single junction, refillable, combination pH
electrode. Use: laboratory, vials.
HI1331B Glass body, semimicro, single junction, refillable, combination pH
electrode. Use: flasks.
HI1230B Plastic body (PEI), double junction, gel filled, combination pH
electrode. Use: general, field.
79
ACCESSORIES
HI2031B Glass body, semimicro, conical, single junction, refillable,
combination pH electrode. Use: semisolid products.
HI1332B Plastic body (PEI), double junction, refillable, combination pH
electrode. Use: general purpose.
HI1413B Glass body, single junction, flat tip, Viscolene, non refillable,
combination pH electrode. Use: surface measurement.
FC100B Plastic body (PVDF), double junction, refillable, combination pH
electrode. Use: general purpose for food industry.
FC200B Plastic body (PVDF), single junction, conical, Viscolene, non
refillable, combination pH electrode. Use: meat & cheese.
80
ACCESSORIES
FC210B Glass body, double junction, conical, Viscolene, non refillable,
combination pH electrode. Use: milk, yogurt.
FC220B Glass body, triple ceramic, single junction, refillable, combination pH
electrode. Use: food processing.
FC911B Plastic body (PVDF), double junction, refillable with built-in
amplifier, combination pH electrode. Use: very high humidity.
ORP ELECTRODES HI3131B Glass body, refillable, combination platinum ORP
electrode. Use: titration.
HI3230B Plastic body (PEI), gel filled, combination platinum ORP electrode.
Use: general purpose.
81
ACCESSORIES
HI4430B Plastic body (PEI), gel filled, combination gold ORP electrode. Use: general purpose.
Consult the Hanna Instruments General Catalog for more electrodes with screw- type or BNC connectors.
EXTENSION CABLE FOR SCREW-TYPE ELECTRODES (SCREW TO BNC ADAPTER)
HI7855/1 HI7855/3
Extension cable 1 m (3.3′) long Extension cable 3 m (9.9′) long
HI 7855 SERIES CABLE CONNECTORS CONNECTOR AND 3.0 mm (0.12″) CABLE WITH BNC
CONNECT TO SCREW TYPE ELECTRODES
CONNECT TO THE BNC SOCKET OF THE METER
OTHER ACCESSORIES
HI710005/8 Voltage adapter from 120 Vac / 12 Vdc 800 mA (USA plug)
HI710006/8 Voltage adapter from 230 Vac / 12 Vdc 800 mA (European plug)
HI76404W Electrode holder
HI8427
pH and ORP electrode simulator with 1 m (3.3′) coaxial cable ending in female BNC connectors
HI931001
pH and ORP electrode simulator with LCD and 1 m (3.3′) coaxial cable ending in female BNC connectors
HI7662-W Temperature probe with 1 m (3.3′) cable
HI92000
Windows® compatible software
HI920013 USB cable
MAN5222 05/19
82
Certification All Hanna Instruments conform to the CE European Directives.
Disposal of Electrical & Electronic Equipment. The product should not be
treated as household waste. Instead hand it over to the appropriate collection
point for the recycling of electrical and electronic equipment which will
conserve natural resources. Disposal of waste batteries. This product contains
batteries, do not dispose of them with other household waste. Hand them over
to the appropriate collection point for recycling. Ensuring proper product and
battery disposal prevents potential negative consequences for the environment
and human health. For more information, contact your city, your local
household waste disposal service, the place of purchase or go to
www.hannainst.com.
Recommendations for Users
Before using this product, make sure that it is entirely suitable for your specific application and for the environment in which it is used. Any variation introduced by the user to the supplied equipment may degrade the instrument’s performance. For yours and the instrument’s safety, do not use or store the instrument in hazardous environments.
Warranty
HI5221 and HI5222 are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to instructions. Electrodes and probes are warranted for six months. This warranty is limited to repair or replacement free of charge. Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number and the nature of the problem. If the repair is not covered by the warranty, you will be noticed of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization (RGA) number from the Technical Service department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection.
Hanna Instruments reserves the right to modify the design, construction or appearance of it’s products without advance notice.
World Headquarters
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive
Woonsocket, RI 02895 USA www.hannainst.com
MAN5222
Printed in ROMANIA
References
- PC Compatible Software Versions | Hanna Instruments
- pH Meters, Photometers, Titrators, Controllers - Hanna Instruments