HANNA instruments HI5221 pH/mV Temperature Bench Meters Instruction Manual

HI5221 pH/mV Temperature Bench Meters

Product Information: HI5221 & HI5222 pH/mV/ISE/Temperature

Bench Meters

The HI5221 and HI5222 are pH/mV/ISE/Temperature bench meters
manufactured by Hanna Instruments. These meters are designed to
provide accurate measurements of pH, mV, ISE, and temperature in
various laboratory applications. The meters come with a liquid
crystal display (LCD) and capacitive touch keypad for easy
operation. They also have USB connectivity for data transfer and
recording.

Specifications:

• Range: -2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000
  pH

• Resolution: 0.1 pH / 0.01 pH / 0.001 pH, 0.1 mV

• Relative mV offset range pH Calibration

• ISE Calibration

• Temperature User Calibration Input Channel

• Temperature Compensation GLP

• Logging Record Logging Interval Logging Type

• pH Electrode Temperature Probe

• PC Interface Input Impedance

• Display Power Supply Enviroment Dimensions Weight

• Up to five points calibration, 8 standard buffers available (pH
  1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45), and 5 custom
  buffers

• Up to five points calibration, seven fixed standard solutions
  available, for each measurement unit, and five custom
  solutions

• 1 pH/mV, 2 pH/mV/ISE

• Manual or Automatic

Product Usage Instructions:

1. Remove the instrument from the packing material and examine it
   carefully to make sure that no damage has occurred during
   shipping.

2. If there is any damage, please contact your local Hanna
   Instruments Office.

3. Connect the power adapter to the power socket of the
   meter.

4. Connect the BNC electrode connector for pH/ORP/ISE measurements
   to the appropriate channel (Channel 1 for HI5221, Channel 1 or 2
   for HI5222).

5. Connect the temperature probe socket to the appropriate channel
   (Channel 1 for HI5221, Channel 1 or 2 for HI5222).

6. Turn on the meter using the ON/OFF switch.

7. Select the desired measurement mode (pH, mV, ISE, or
   temperature) using the functional keys on the keypad.

8. Perform calibration if necessary using the virtual keys
   assigned to the highlighted channel.

9. Obtain measurements by placing the electrode/probe in the
   sample and reading the values on the LCD display.

10. Record and transfer data using the USB connectivity
    feature.

INSTRUCTION MANUAL

HI5221 & HI5222
pH/mV/ISE/Temperature Bench Meters

Dear Customer

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using this instrument. This manual will provide you with the necessary information for correct use of this instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or view our worldwide contact list for a Hanna Instruments representative near you at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ………………………………………………………………………………….. 4 GENERAL DESCRIPTION …………………………………………………………………………………………. 5 FUNCTIONAL DESCRIPTION …………………………………………………………………………………….. 6 SPECIFICATIONS ………………………………………………………………………………………………….. 9 OPERATIONAL GUIDE ……………………………………………………………………………………………. 10 DISPLAYING MODES…………………………………………………………………………………………….. 11 SYSTEM SETUP ……………………………………………………………………………………………………. 15 pH SETUP ………………………………………………………………………………………………………….. 21 mV SETUP …………………………………………………………………………………………………………. 35 ISE SETUP (HI5222 only) …………………………………………………………………………………….. 37 pH CALIBRATION …………………………………………………………………………………………………. 45 pH MEASUREMENT ………………………………………………………………………………………………. 49 mV & Relative mV MEASUREMENTS …………………………………………………………………………. 51 ISE CALIBRATION (HI5222 only) …………………………………………………………………………….. 53 ISE MEASUREMENT (HI5222 only) …………………………………………………………………………. 56 TEMPERATURE CALIBRATION ………………………………………………………………………………….. 63 LOGGING ……………………………………………………………………………………………………………. 64 PC INTERFACE …………………………………………………………………………………………………….. 68 ADDITIONAL INFORMATION ……………………………………………………………………………………. 69 pH BUFFER TEMPERATURE DEPENDENCE ………………………………………………………………….. 72 ELECTRODE CONDITIONING AND MAINTENANCE ………………………………………………………….. 73 TROUBLESHOOTING GUIDE ……………………………………………………………………………………. 75 TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ……………………………………………….. 76 ACCESSORIES ……………………………………………………………………………………………………… 77
3

PRELIMINARY EXAMINATION

Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, please contact your local Hanna Instruments Office. The meters are supplied complete with:
· HI1131B Glass body Combination pH Electrode · HI7662-W Temperature probe · HI7082S Electrolyte Solution · HI76404W Electrode Holder · pH Calibration Solutions Kit · Capillary dropper pipette · 12 Vdc Power Adapter · Instruction Manual and Quick Reference Guide · Certificate HI5221-01 and HI5222-01 are supplied with 12 Vdc/120 Vac adapter. HI5221-02 and HI5222-02 are supplied with 12 Vdc/230 Vac adapter. Note: Save all packing material until you are sure that the instrument works properly. Any defective item must be returned in the original packing with the supplied accessories.
4

GENERAL DESCRIPTION

HI5221 and HI5222 are professional bench meters with a color graphic LCD for pH (with Calibration Check), ORP (Oxidation Reduction Potential), ISE (HI5222 only) and Temperature measurements. The display can be configured as a single channel or dual channel display (HI5222 only). Each channel can be configured as pH, mV, Relative mV or ISE (HI5222 only). The main features of the instruments are: · Single (HI5221) or two (HI5222) input channels; · Capacitive touch keypad; · pH calibration using up to five pH buffers: choose from the list of Hanna Instruments buffers
(pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) or custom buffers (up to 5 custom buffers); · ISE calibration using up to five standards: choose from the list of nominal standard values (e.g. for ppm: 0.010, 0.100, 1.00, 10.0, 100, 1000, 10000 ppm) and/or from 5 user-supplied custom standard values (up to 5 custom solutions); · AutoHold feature to freeze first stable reading on the LCD; · Two selectable alarm limits; · User Selectable logging modes: Automatic logging, Manual logging with or without AutoHold; · Continuous Lot logging directly on meter, with selectable log interval: Store up to 100,000 total data points; · Up to 100 logging lots; a single lot can have up to 50,000 data points; HI5222 (same for each measurement channel). Log files include GLP information; · Basic Measurement can be viewed with detailed GLP information, or with a Graph or a Log History; · User-friendly interface on large color graphic LCD (240 x 320 pixels) with user selectable color palette; · PC interface via USB; download logged data to PC or use for Real time logging (HI92000 PC application required); · Dedicated Help key with contextual message; · Profile feature: store up to ten different user setup (HI5222 – five on each channel).
5

FUNCTIONAL DESCRIPTION

HI5221 DESCRIPTION FRONT PANEL
REAR PANEL

1. Liquid Crystal Display (LCD) 2) Capacitive touch keypad 3) ON/OFF switch 4) Power adapter socket 5) USB connector 6) BNC electrode connector for pH/ORP measurements 7) Temperature probe socket 8) Reference input socket 6

FUNCTIONAL DESCRIPTION

HI5222 DESCRIPTION FRONT PANEL
REAR PANEL

1. Liquid Crystal Display (LCD) 2) Capacitive touch keypad 3) ON/OFF switch 4) Power adapter socket 5) USB connector 6) BNC electrode connector for pH/ORP/ISE measurements (Channel 1) 7) Temperature probe socket (Channel 1) 8) Reference input socket (Channel 1) 9) Reference input socket (Channel 2) 10) Temperature probe socket (Channel 2) 11) BNC electrode connector for pH/ORP/ISE measurements (Channel 2)
   7

FUNCTIONAL DESCRIPTION

KEYBOARD DESCRIPTION FUNCTION KEYS
To enter/exit calibration mode.
To select the desired measurement mode, pH, mV, Rel mV (or ISE ­ HI5222 only).
To enter Setup (System, pH, mV or ISE) and to access Log Recall function.

To obtain general information about the selected option/operation.

VIRTUAL KEYS

The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow

you to perform the displayed function, depending on the current menu (e.g. for HI5222

,

and

in Measure mode).

Note: All the virtual keys are assigned to the highlighted channel (HI5222 only).

LCD GENERAL DESCRIPTION

8

SPECIFICATIONS

Range
Resolution
Accuracy @25 °C / 77 °F
Relative mV offset range pH Calibration
ISE Calibration
Temperature User Calibration Input Channel
Temperature Compensation GLP
Logging Record Logging Interval Logging Type
pH Electrode Temperature Probe
PC Interface Input Impedance
Display Power Supply Enviroment Dimensions
Weight

HI5221

HI5222

-2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000 pH

±2000.0 mV

–

e.g. 10-7 to 10 M, 0.005 to 105 ppm, 5·10­7 to 5·107 conc.

-20.0 to 120.0 °C / -4.0 to 248.0 °F / 253.2 to 393.2 K

0.1 pH / 0.01 pH / 0.001 pH

0.1 mV

–

1, 2, 3 significants digit

0.1 °C / 0.1 °F / 0.1 K

±0.1 pH / ±0.01 pH / ±0.002 pH ±1 last significant digit

±0.2 mV ±1 last significant digit

–

±0.5% (monovalent ions) ±1% (divalent ions)

±0.2 °C / ±0.4 °F / ±0.2 K

±2000.0 mV

Up to five points calibration, 8 standard buffers available (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45), and 5 custom buffers

Up to five points calibration,

–

seven fixed standard solutions available, for each measurement unit,

and five custom solutions

3 points (0, 50, 100 °C)

1 pH/mV

2 pH/mV/ISE

Manual or Automatic

Electrode offset / slope, calibration points, calibration time stamp, date, time and buffers/standards used

Up to 100 lots, 50,000 records max/lot / maximum 100,000 data points/channel

14 selectable between 1 second and 180 minutes

Automatic, Manual and Auto Hold

HI1131B

HI7662-W

Opto-isolated USB 1012

Color Graphic LCD (240 x 320 pixels)

12 Vdc adapter

0 – 50 °C / 32 – 122 °F / 273 – 323 K max. 95% RH non-condensing

160 x 231 x 94 mm (6.3 x 9.1 x 3.7″)

1.2 Kg (2.6 lbs.)

9

OPERATIONAL GUIDE

POWER CONNECTION Plug the 12 Vdc adapter into the power supply socket.
Notes: These instruments use non-volatile memory to retain the pH, ISE calibrations and all other settings, even when unplugged. Make sure a fuse protects the main line. ELECTRODE AND PROBE CONNECTIONS For pH or ORP measurements, connect a pH/ORP electrode with internal reference to the BNC connector located on the rear panel of the instrument (to the desired channel – HI5222 only). For ISE measurements, (HI5222 only) connect, to the desired channel, an ISE electrode with internal reference to the BNC connector located on the rear panel of the instrument. For electrodes with a separate reference, connect the electrode’s BNC to the BNC connector and the electrode’s reference to the reference input socket. For temperature measurement and automatic temperature compensation, connect the temperature probe to the appropriate socket (to desired channel – HI5222 only). INSTRUMENT START UP · Please ensure that the capacitive keypad is not covered by hand or other objects at the meter power on. · Turn the instrument on from the power button located on the rear panel of the instrument. · Please wait until the instrument finishes the initialization process. Note: It is normal for the loading process to take a few seconds. If the instrument doesn’t display the next screen, restart the meter using the power button. If the problem persists, contact your local Hanna Instruments Office.
10

OPERATIONAL GUIDE

CHANNEL SELECTION (HI5222 only)

· Press

while in Measure mode to access channel

selection menu.

Four available options will be displayed: Channel

1, Channel 2, or multi-channel with the first or

the second channel focused. The “Choose Channel

Configuration” message is displayed in the Reminder

messages area.

· Select the desired option by pressing the appropriate

key:

,

,

or

.

The instrument will display in the selected option Measure mode.

For each measurement mode (pH, mV, Rel mV or ISE) the following display configurations are available: Basic, Good Laboratory Practice (GLP) (pH, ISE only), Graph and Log History.

Basic

The main measured value and it’s units are displayed on the LCD, along with the temperature

value, temperature probe status and basic calibration information when this option is accessed.

To choose the Basic display mode:

· Press

while in Measure mode. The “Choose Display Configuration” message will be

displayed in the Reminder messages area.

· Press

. The instrument will display the basic information for the selected measurement

mode.

DISPLAYING MODES

11

DISPLAYING MODES

GLP

Detailed GLP data will be displayed on the LCD for pH Measure and ISE Measure modes only when this option is selected: Last Calibration date and time, Offset and Slope values, Calibration Buffers/Standards and general information regarding the buffers/standards: the calibration temperature, temperature compensation mode, date and time. For pH Measure, the Electrode Condition is also displayed on the LCD in percent.

Note: If a one point pH calibration is performed or the current calibration does not include at

least two consecutive standard buffers of pH 4.01, 7.01 (6.86) and 10.01 (9.18) buffers, the

Electrode Condition will be unknown. Electrode condition remains active for 24 hours after a

calibration.

To access the GLP display option:

· Press

while in Measure mode. The “Choose Display Configuration” message will be

displayed in the Reminder messages area.

· Press

. The instrument will display the detailed GLP data.

12

DISPLAYING MODES

Graph

The on-line graph with currently logged values (pH, mV, Rel mV, or ISE vs. Seconds) will be

displayed when this option is selected.

If there is no active log, the previously logged data for the selected parameter will be shown.

To access the off-line / on-line graph:

· Press

while in Measure / Logging mode. The “Choose Display Configuration” message

will be displayed in the Reminder messages area.

· Press

. Press

to begin an online graph.

To Zoom Graph

· Press

then

.

and

will appear in virtual keys.

· Use

and

to move along the X (Time) axis of the graph.

· Press

to access the zoom menu for Y axis. Use

or

for zooming Y

(parameter) axis.

· Press

to return to the main menu.

When the off-line graph is displayed:

· Use the arrow keys to move along the X (Time) and Y (parameter) axes of the graph.

· Press

to access the zoom menu for X and Y axes. Use

or

/

/

/

to switch between the active zooming axes. Press

or

to

zoom the selected axis.

Note: While in zoom graph menu the

key is not accessible.

Press

to return to the main menu.

13

DISPLAYING MODES

Log History

The measurement, along with Log History, will be visible when this option is selected:

1. The last stored logged data (Not actively logging) or

2. The last data logged from an active logging lot or

3. An empty display – NO LOTS saved, NOT currently logging

The log history list also contains the appropriate mV values, the logged temperature, the temperature probe status source, as well as the records time stamp.

To access the Log History display option:

· Press

while in Measure mode. The “Choose Display Configuration” message will be

displayed in the Reminder messages area.

· Press mode.

. The instrument will display the log history regarding the selected Measure

Notes: When an alarm condition is active, all logged records will have an exclamation mark “!” If logged in Auto Hold, logged records will have an “H”.

If another Measure mode is selected, the Log History will reset. If the temperature unit is changed, all logged temperature values will be automatically displayed in the new temperature unit.
14

SYSTEM SETUP

The System Setup menu allows the user to customize the user interface, view meter information, set the external serial communication interface and to restore the manufacturer settings.

Accessing System Setup

· Press

while in Measure mode.

· Press

. The system setup options will be

displayed on the LCD.

To access a System Setup option:

· Use

or

to highlight the desired

option.

· Press

to access the selected option.

The following is a detailed description of the System Setup option screen:
Beeper
This option allows the user to turn an acoustic warning signal on or off. This function can be used to signal 4 different events: a stable signal, an alarm state, when every key is pressed or when an incorrect key is pressed. Enable (or disable) the Beeper for these events. Disabling the Beeper will stop audible signals.

15

SYSTEM SETUP

Saving Confirmation
Enable this option to force confirmation of a change made to a setting in GLP data option field or a Sample ID name. If Saving Confirmation is enabled, the user will have to accept the change with a key stroke. If Saving Confirmation is disabled, the changes made to these fields change automatically without asking for confirmation

GLP Data
Use this option to customize logging GLP information with specific identification data. When enabled, these ID tags will be included in the GLP section of all data logs for all modes of operation and both channels (HI5222). Each data field can use up to 10 characters. The five available fields are:
Operator ID : used to add the name of the operator
Instrument ID : used to name an instrument with a discrete name, location or number
Company Name : used to include the Company ID to the GLP data field.
Additional Info : two data fields are available for general notes or notations.

To add the GLP Data:

· Press

while in Measure mode.

· Press

.

· Use

or

to select the GLP Data option.

· Press

and use

or

to highlight

the desired option.

· Press

to edit the desired information. The Text

Editor menu will be displayed on the LCD.

· Enter the desired information by accepting the

highlighted character which is added to the text bar,

using

. The

and

keys help the

user to select the desired character. It is also possible to delete the last character by positioning the

cursor on the Backspace character ( ) and pressing

.

16

SYSTEM SETUP

· Press

to return to the GLP Data options. If the Saving Confirmation is enabled, press

to accept the modified option,

to escape without saving or

to return

to the editing mode. Otherwise, the modified options are saved automatically.

Date & Time Set the current date & time and the format in which they appear.

Set Date and Time This option allows the user to set the current date (year/month/day) and time (hour/minute/second).

Notes: Only years starting with 2000 are accepted.

The time is set using the selected time format. For 12 Hour time format only, the AM/PM

can also be selected with

or

.

Set Time Format Choose between 12-Hour (AM/PM) time format or 24-Hour time format.

Set Date Format
Choose the desired date format from 7 available options: DD/MM/YYYY, MM/DD/YYYY, YYYY/MM/DD, YYYY-MM-DD, Mon DD, YYYY, DD-Mon-YYYY or YYYY-Mon-DD.

To set the Date & Time:

· Press

while in Measure mode.

· Press

.

· Use

or

option.

to select the Date & Time

· Press

and use

or

to

highlight the desired option you want to modify.

· Press

to confirm your selection. Use

/

to select next/previous entry

to be edited. Press

and use

or

to set the desired value, then press

to save the modified value (for Set Date and Time

option). For the Set Date Format option press

to confirm your selection and select one of

the displayed options with

or

.

· Press

to confirm your selection and return to the Date & Time options or press

to cancel operation.

17

SYSTEM SETUP

Note: If the time is changed with more than one hour before last pH/ISE calibration, a pop-up warning will appear on the LCD, notifying the user that a date/time conflict has occurred and some time-dependent modes could work improperly (e.g. Measure, GLP, Log).

LCD Setup

This option allows the user to set the Contrast, the Backlight of the LCD and the Backlight Saver. The Contrast parameter can be adjusted within 7 steps, while the Backlight parameter within 8 steps. The Backlight Saver can be set from 1 to 60 minutes or it can be OFF (disabled). All the changes are visible on the LCD for each parameter.

Note: If the instrument backlight turns off after the set period of time, press any key to turn it back on.

To set the LCD Setup:

· Press

while in Measure mode.

· Press

.

· Use

or

to select the LCD Setup option.

· Press

and use

parameter.

key to highlight the desired

· Use

or

to adjust the contrast / backlight

or to set the desired backlight saver time.

· Press

to confirm the modified options and return

to the System Setup menu.

Color Palette

This option allow the user to choose desired color palette.

To select the Color Palette:

· Press

while in Measure mode.

· Press

.

· Use

or

option.

to select the Color Palette

Color 1 White background blue text

Color 2 Blue background white text

Color 3 White background black text

Color 4 Black background white text

18

SYSTEM SETUP

· Press

and use

or

to highlight the desired color.

· Press

to confirm your selection and return to the System Setup menu or press

to return to the System Setup menu without changing.

Language

This option allows the user to choose the desired language in which all information will be displayed.

To select the Language:

· Press

while in Measure mode.

· Press

.

· Use

or

option.

to select the Language

· Press

and use

or

to

highlight the desired language.

· Press

to confirm your selection and return to

the System Setup menu or press

to return to

the System Setup menu without changing.

Serial Communication

This option allows the user to set the desired speed for the serial communication (baud rate) in bps. The meter and the PC program must have the same baud rate.

To set the Serial Communication:

· Press

while in Measure mode.

· Press

.

· Use

or

to select the Serial

Communication option.

· Press

and use

or

to

highlight the desired baud rate.

· Press

to confirm your selection and return to

the System Setup menu or press

to return to

the System Setup menu without changing.

19

SYSTEM SETUP

Meter Information

This option provides general information about the instrument serial number (each instrument has a unique identification serial number), the software version and the factory calibration date and time (for mV and temperature).

Note: All instruments are factory calibrated for mV and temperature. One year after factory calibration, a warning message “Factory Calibration Expired” will be displayed when powering up the instrument. The instrument will still function, however, it should be taken to the nearest Hanna Instruments Customer Service for factory calibration.

To view the Meter Information:

· Press

while in Measure mode.

· Press

.

· Use

or

to select the Meter

Information option.

· Press

to acces the Meter Information menu.

· Press

to return to the System Setup menu.

Restore Factory Settings

This option allows the user to erase all user settings and reset the instrument to the default factory settings in pH mode.

To restore the Factory Settings:

· Press

while in Measure mode.

· Press

.

· Use

or

Settings option.

to select the Restore Factory

· Press

to confirm your selection. A pop-up

menu will be displayed, asking for confirmation.

· Press

to confirm your selection and return to

the System Setup or press

to return to the

System Setup menu without restoring defaults.

· Press

to return to Measure mode.

20

SYSTEM SETUP

Software update
This function allows the user to update instrument software. In order to start the PC upgrade application, you need to select the proper baud rate, the software update package and start the update.

pH SETUP

The pH Setup menu allows the user to set the parameters associated with pH measurement and calibration. These parameters can be set specifically for each channel (HI5222 only). The settings will be applied only to the active channel.

Accessing pH Setup

· Press

while in Measure mode and then

to select pH range for the desired channel.

· Press menu.

and then

to access pH Setup

To access a pH Setup option:

· Use

or

option.

to highlight the desired

· Press

to access the selected option.

The following is a detailed description of the pH Setup option screens.

21

pH SETUP

Profile

This option opens the Profile manager. Enabling Profile allows the user to Save, Load or Delete an application Profile. The Profile option allows the user to store up to ten separate profile applications (HI5222 – five profiles for each channel). Each Profile can be named and recalled at a moment’s notice. A profile is a sensor setup complete with measurement units, logging and display preferences, calibration standards (Buffer or Standards including custom), setup of the Display screen for measurement (i.e. single, dual, graphing, GLP) and any other sensor configuration. Once saved, the exact same profile can be used at another time. This is a handy feature if the meter is used occasionally for additional applications because it saves time in the setup of the meter and ensures that the same procedure will be used.
To save the measurement configuration for pH mode:

· Press

and use

or

to highlight Profile.

· Press

/

to enable / disable this feature.

The available options are: Save Profile: save the current profile. Save Profile As…: save current profile using a specific name. Load Profile: load from available profiles. Delete Profile: delete a profile.

Save Current Profile

To save the current profile:

· Use

or

to select Save Profile As…

· Press

. The Text Editor box will be displayed on the LCD.

· Enter the desired profile name by using

and

to highlight the desired character

and then press

to add it to the text bar. It is also possible to delete the last character by

positioning the cursor on the Backspace character( )and pressing

.

· Press

to return to the Profile options.

· Use Save Profile to save changes made to a presently used Profile. Changes will overwrite

existing configurations.

22

pH SETUP

· Select Load Profile to select a profile to use from the list of saved profiles. Highlight the desired

profile and press

.

· Select Delete Profile to remove a selected profile from the saved list.

Highlight the profile and press .

Temperature
The temperature has a direct influence on pH. This option allows the user to choose the temperature source and units, as well as the desired manual temperature for manual temperature compensation mode. Temperature Source (HI5222 only)
If using a temperature probe, Automatic Temperature Compensation will be performed relative to the displayed temperature, with the “ATC” indicator displayed on the LCD. For the HI5222, one temperature probe can be used for both measurement channels if desired. Select the source by selecting Manual, Channel 1 or Channel 2 . If no temperature probe is detected, Manual Temperature Compensation will be performed, with the “MTC” indicator on the LCD. Temperature Unit
The desired temperature unit can be chosen (Celsius, Fahrenheit or Kelvin degrees) and the meter will automatically make the conversion for the selected unit.
If no temperature probe is connected, the desired temperature can be set manually. The default setting is 25.0 °C. If the measured temperature is different, the value can be manually adjusted to obtain an accurate pH measurement.

To set one of the Temperature options:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Temperature

option.

· Press

and use

or

to highlight

the desired Temperature option you wish to modify.

· Press

and use

or

to highlight

the desired option (for Temperature Source & Unit

options) or use

or

to adjust the

temperature value between the displayed limits (for

Manual Temperature option).

23

pH SETUP

· Press

to confirm your selection (for Temperature Source & Unit options) or press

to save the current value (for Manual Temperature option). Otherwise, press

to cancel

operation.

Calibration This option allows the user to setup desired buffers and calibration reminder.

Buffer Entry Type Three settings are available for the pH buffers used for electrode calibration: Automatic – the instrument automatically selects the closest buffer to the measured pH value from the predefined buffers chosen in the option Edit Buffer Group. Semiautomatic – the instrument automatically selects the closest buffers to the measured pH value from all available buffers and you can choose the one used to be, from standard and custom buffers. Manual Selection – the desired pH buffer is manually selected from all available buffers (standard and custom).

To set the Buffer Entry Type:

· Press

while in pH Measure mode.

· Press

.

· Use

or

option.

to select the Calibration

· Press

and use

or

to

highlight the Buffer Entry Type option.

· Press

and use

or

to

highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

1st Cal. Point Two options are available for the 1st Cal. Point parameter: Point and Offset. Point: A new buffer can be added to an existing calibration. The electrode slope will be reevaluated with the addition of this buffer (normal operation). Offset: The new buffer calibration point can create a constant offset to all existing pH calibration data (existing calibration must have a minimum of two pH buffers).
24

pH SETUP

To set the 1st Cal. Point:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Calibration

option.

· Press

and use

or

to

highlight the 1st Cal. Point option.

· Press

/

as desired.

· Press

to return to previous menu.

Edit Custom Buffers

If special custom pH buffers are required during calibration, the Edit Custom Buffers option is available. Up to five pH custom buffers can be added. If a custom buffer is used, the user must verify it’s value at temperature of calibration.

To edit/set the Custom Buffers:

· Press

while in pH Measure mode.

· Press

.

· Use

or

option.

to select the Calibration

· Press

and use

or

to

highlight the Edit Custom Buffers option.

· For a previous set value, press confirm the setting by pressing buffer.

to set the custom buffer value to “—-” if desired and

, otherwise press

to edit the selected custom

· While in edit custom buffer menu press

to set the custom buffer value to 7.000 pH and

then use

or

to set the desired custom buffer value.

25

pH SETUP

· Press

to exit custom buffer edit menu. If the Saving Confirmation is enabled, press

to accept the modified option,

to escape without saving or

to return

to the editing mode. Otherwise, the modified option is saved automatically.

· Use

key to select the next custom buffer to be set or press

to return to Calibration

options.

Edit Buffer Group

Accessing this option, the user can edit the desired group of five pH buffers for automatic buffer recognition (Automatic Buffer Entry Type). If the Buffer Group already contains five pH buffers, at least one pH buffer has to be removed in order to add another buffer.

To edit/set the Buffer Group:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Calibration option.

· Press

and use

or

to highlight

the Edit Buffer Group option.

· Press

and use

and

to choose the pH buffer to be included in the buffer

group.

· Press

or

to add/remove the selected pH buffer to/from the buffer group.

· Press

to return to Calibration options and to save the changes.

Calibration Reminder

This option allows the user to select a calibration reminder schedule if desired. Three options are available for the calibration reminder: Daily, Periodic or Disabled.

To set the Calibration Reminder:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Calibration option.

26

pH SETUP

· Press · Press · Press

and use

or

to highlight the Calibration Reminder option.

and use

or

to highlight the desired option.

to confirm your selection or press

to cancel operation.

Set Reminder Period Schedule the calibration reminder timing with this option (verify Daily or Periodic is set for Calibration Reminder). If a Daily reminder is desired, set the time of day you wish the reminder to occur. If a Periodic reminder is desired, schedule time in days, hours and/or minutes after the last calibration for the reminder to occur. To set the Reminder Period:

· Press

while in pH Measure mode.

· Press

.

· Use

or

option.

to select the Calibration

· Press

and use

or

to highlight the Set Reminder Period option.

· Press

and use

/

to select next/previous entry to be edited.

· Press

and use

or

to set the desired value, then press

to save

the modified value.

· Press

to return to the Calibration options. If the Saving Confirmation is enabled, press

to accept the modified option,

to escape without saving or

to the editing mode. Otherwise, the modified option is saved automatically.

to return

Clear Calibration
This feature deletes the pH electrode calibration for the attached electrode and channel (HI5222). A default meter calibration will replace the actual electrode calibration until a new electrode calibration is made.

27

pH SETUP

To clear Calibration:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Calibration

option.

· Press

and use

or

to

highlight the Clear Calibration option.

· Press

to clear calibration. A pop-up menu

will be displayed asking for confirmation (when a

calibration is available).

· Press

to confirm or press

to escape

without saving and return to the Calibration options.

Sample ID
This option allows the user to assign an identification number/name. Two Sample ID options are available: ID Increment and Edit Sample ID.

ID Increment

Two choices are available for the sample ID:

None – the sample ID will be fixed and it can be set alphanumeric (see Edit Sample ID).

Automatic – the sample ID will automatically increment by one for each new log lot.

To set the ID Increment mode:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Sample ID option.

· Press

and use

or

to highlight the ID Increment option.

· Press

/

as desired.

· Press

to return to previous menu.

28

pH SETUP

Edit Sample ID

This option allows the user to edit the sample ID. Note: The ID Increment mode must be set to None, to use this feature.

To edit the Sample ID:

· Press

while in pH Measure mode.

· Press

.

· Use

or

option.

to select the Sample ID

· Press

and use

or

to

highlight the Edit Sample ID option.

· Press

to confirm your selection.

· If the selected increment is None, the Text Editor menu will be displayed on the LCD, allowing you

to enter the desired sample number/name by accepting the highlighted character which is added

in the text bar, using

. The

and

keys help the user to select the desired

character.

It is also possible to delete the last character; position the cursor on the Backspace character and

press

.

· Press

to return to Sample ID options. If the

Saving Confirmation is enabled, press

to accept the modified option,

to escape

without saving or

to return to the editing

mode.

Otherwise, the modified options are saved

automatically.

· If the selected increment mode is Automatic, the desired

sample ID value can be set using

or

· Press

to save the current value or press

to cancel operation.

29

pH SETUP

Stability Criteria

This option allows the user to select the signal stability criteria for the measured parameter (pH, mV, ISE):

Fast – this setting will give faster results with less accuracy.

Medium – this setting will give medium speed results with medium accuracy.

Accurate – this setting will give slower results with high accuracy.

To set the Stability Criteria:

· Press

while in pH Measure mode.

· Press

.

· Use

or

Criteria option.

to select the Stability

· Press

and use

or

to

highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

Reading Mode

This option allows the user to select between Direct and

Direct/AutoHold pH reading mode. If choosing the AutoHold option, the current reading can be frozen

on the LCD when

is pressed and the stability criteria is reached.

To set the Reading Mode:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Reading Mode

option.

· Press

/

to select Direct / AutoHold

option as desired.

· Press

to cancel operation.

30

pH SETUP

Log Note: See section Logging for available types of logging.
This option allows the user to edit the logging settings: Logging Type, Logging Data Configuration, Sampling Period and New Lot.

Logging Type

Three logging types are available: Automatic, Manual and Auto Hold.

Automatic – the measurement data is logged automatically at predefined time intervals;

Manual – a snapshot of the displayed measurement data is logged with time stamp when the user manually presses Log;

Auto Hold – this is configured along with the Direct/AutoHold Reading Mode to take a snapshot of

stable measurement data. Press

to initiate a logging session. Press

to initiate an

Auto Hold event. The log occurs automatically once measurement stability is reached. This type log

removes subjective data, as it only captures stable measurements.

To set the Logging Type:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Log option.

· Press

and use

or

to

highlight the Logging Type option.

· Press

and use

or

to

highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

Logging Data Configuration
This option allows the user to select which additional parameters will accompany a log report: Date/Time, Calibration Data, Sample ID, Instrument ID, Operator ID, Company Name, Additional Info 1 and Additional Info 2.

31

pH SETUP

To set the Logging Data Configuration:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Log option.

· Press

and use

or

to highlight

the Logging Data Configuration option.

· Press

and use

or

to highlight

the desired parameter to be logged in file.

· Press

to enable the parameter or

to

disable it.

· Press

to return to previous menu.

Sampling Period

This option allows the user to select the desired sampling period for automatic logging type.

To set the Sampling Period:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Log option.

· Press

and use

or

the Sampling Period option.

to highlight

· Press

and use

or

to select the

desired option.

· Press

to confirm your selection or press

to cancel operation.

New Lot This option is used to create a new lot when manual logging is used.
Note: If New Lot option is accessed and the Logging Type is Automatic, a warning message appears on the LCD informing the user that a new lot can be created only if the Logging Type is set as Manual.

32

pH SETUP

To generate a New Lot:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Log option.

· Press

and use

or

to highlight the

New Lot option.

· Press

to generate a new manual lot. A pop-up

menu will be displayed asking for confirmation.

· Press

to confirm or press

to escape

without saving and return to the Log options.

Alarm

This option allows the user to select the alarm settings: Alarm State and Alarm Limits. If the Alarm option is enabled, a continuous double beep will be heard, along with the “Alarm” indicator blinking on the LCD, each time the set limits in Measure mode are exceeded.
Note: Alarm Beeper must be set On for audible beep to be heard. See: System Setup > Beeper > Alarm.

Alarm State

Three settings are available for the Alarm State option:

Disabled – the alarm will be disabled.

Inside Limits – the alarm state will trigger when the measured value is inside the set limits.

Outside Limits – the alarm state will trigger when the measured value is outside the set limits.

To set the Alarm State:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Alarm option.

· Press

and use

or

to

highlight the Alarm State option.

· Press

and use

or

to highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

Alarm Limits

This option allows the user to set the alarm limits for the measured value. Note: The Alarm High value can not be lower than the Alarm Low value.

33

pH SETUP

To set the Alarm Limits:

· Press

while in pH Measure mode.

· Press

.

· Use

or

to select the Alarm option.

· Press

and use

or

to

highlight the Alarm Limits option.

· Press

and use

/

to select

next/previous entry to be edited.

· Press

and use

or

to set the

desired value, then press

to save the modified

value.

· Press

to return to the Alarm options. The

modified option is saved automatically.

Isopotential Point

This option allows the user to edit the isopotential point of the electrode used for pH measurements. The isopotential point is the mV reading for an electrode at which temperature has no effect on the measurement. The ideal electrode has an isopotential point of 0.0 mV and 7.00 pH, while an actual electrode typically deviates slightly from the ideal values.

If the actual isopotential pH for an electrode is known, it can be set by accessing this option.

Note: If the isopotential point has been modified, recalibration must be performed.

To set the Isopotential Point:

· Press

while in pH Measure mode.

· Press

.

· Use

or

Point option.

to select the Isopotential

· Press using

and set the desired isopotential pH value

or

.

· Press

to save the current value or press

to cancel operation.

34

pH SETUP

pH Resolution

Select the desired pH resolution with this option. Choose from one (X.X), two (X.XX) or three (X.XXX) digits displayed past the decimals.

To set the pH Resolution:

· Press

while in pH Measure mode.

· Press

.

· Use

or

option.

to select the pH Resolution

· Press

and use

or

to highlight

the desired option.

· Press

to confirm your selection or press

to cancel operation.

The mV Setup menu allows the user to set the parameters associated with mV and Relative mV measurements. These parameters can be set specifically for each channel (HI5222 only). The settings will be applied only to the active channel.

Accessing mV Setup

· Press

while in Measure mode and then

or

range for the desired channel.

· Press menu.

and then

to access mV Setup

To access a mV Setup option:

· Use

or

option.

to highlight the desired

· Press

to access the selected option.

The following is a detailed description of the mV Setup option screens.

to select mV / Rel mV

mV SETUP

35

mV SETUP

Profile- See pH Setup section.
Temperature ORP measurements are not temperature compensated, although ORP values can change with temperature (e.g. reference electrode potential changes, sample equilibrium changes). It is important to report ORP values together with the reference electrode used and the temperature of measurement. This option permits selection of the temperature source (HI5222 only) and measurement units.
Temperature Source If using a temperature probe, sample temperature will be displayed with the “ATC” indicator displayed on the LCD. For HI5222 only, the ATC option can be selected from Channel 1 or Channel 2. If no temperature probe is detected, a Manual set value will be displayed (and logged) with the measurement.
Temperature Unit Select the desired temperature unit (Celsius, Fahrenheit or Kelvin degrees) and the meter will automatically convert to the selected unit.
Manual Temperature If no temperature probe is connected, the desired temperature can be manually entered. The default setting is 25.0 °C.
Calibration (Relative mV only) Calibration Reminder This option allows the user to select a calibration reminder schedule if desired. See pH Setup section > Calibration Reminder section for option access details. Set Reminder Period See pH Setup section > Set Reminder Period section.
36

mV SETUP

Clear Calibration

This feature deletes the Relative mV calibration for the selected channel.

· Press

while in Rel mV mode.

· Press

then use

or

to access Calibration option.

· Press

and use

or

to highlight Clear Calibration option.

· Press

to clear calibration. A pop-up menu will be displayed asking for confirmation

(when a calibration is available).

· Press

to confirm or press

to escape without saving and return to the Calibration

options.

Sample ID – See pH Setup section. Stability Criteria – See pH Setup section. Reading Mode – See pH Setup section. Log – See Logging section, or pH Setup section. Alarm – See pH Setup section.

ISE SETUP (HI5222 only)

The ISE Setup menu allows the user to set the parameters regarding ISE measurement and calibration. These parameters can be set specifically for each channel. The settings will be applied only to the active channel.

Accessing ISE Setup

· Press

while in Measure mode and then

to select ISE range for the desired channel.

· Press menu.

and then

to access ISE Setup

To access an ISE Setup option:

· Use

or

option.

to highlight the desired

· Press

to access the selected option.

The following is a detailed description of the ISE Setup option screens.

37

ISE SETUP (HI5222 only)

Profile – See pH Setup section.
Reading Mode This option allows the user to select the desired reading mode: Direct, Direct/AutoHold, Known Addition, Known Subtraction, Analyte Addition and Analyte Subtraction. Four of these Reading Modes are collectively known as Incremental Methods (see ISE Theory section for details). Direct measurements and Direct/AutoHold measurements are also available.
Direct Direct measurements are analogous to taking pH measurements. The ISE is calibrated in ion standards and sample measurements are made directly. The ISE’s manual should be consulted for tips and practices of making Direct measurements. The ion concentration can be read directly from the instrument.
Direct/AutoHold Direct/AutoHold measurements are made similar to Direct measurements. The advantage of using Direct/AutoHold is a measurement that has not reached equilibrium will not be used. Only after the chosen stability criteria has been met will the meter go into the Direct/AutoHold mode. Using Direct/AutoHold removes the subjective nature of stability.
Known Addition In the Known Addition method, a sample is measured with an ISE before and after the addition of a known volume of a standard. The mV difference is then used to calculate the concentration of the ion in the original sample.
Known Subtraction In the Known Subtraction method, a sample is measured with an ISE before and after the addition of a known volume of a reactant standard. The reactant standard reacts with the measured ion in the sample, reducing it’s concentration. The mV difference is then used to calculate the concentration of the ion in the original sample. The stoichiometric ratio between reactant standard and ion in the sample must be known.
Analyte Addition Analyte Addition is similar to the Known Addition method, with the difference being that an aliquot of sample is added to a known volume of standard. Both solutions contain the same measured ion. The standard is measured with an ISE before and after the addition of a known volume of a sample. The ion concentration is then calculated using the difference in mV potential. The sample should increase the concentration of the ion being measured.
38

ISE SETUP (HI5222 only)

Analyte Subtraction
In the Analyte Subtraction method, an aliquot of sample is added to a reactant standard of known concentration and volume. The sample partially reacts with the measured ion. The stoichiometric ratio between standard and sample must be known. The ion concentration is then calculated using the difference in mV potential.

To set the Reading Mode:

· Press

while in ISE Measure mode.

· Press

.

· Use

or

to select the Reading Mode

option.

· Press

and use

or

to

highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

Temperature This option permits the user to configure all parameters related to ISE temperature measurements.
Temperature Source The options are: Manual, Channel 1 or Channel 2. If no temperature probe is detected, a manually set value will be displayed (and logged) with the measurement. If a temperature probe is connected to either channel, it may be selected. The temperature measurement will be displayed and logged with the measurement and may be used for temperature compensation calculation if Temperature Compensation is enabled.
Temperature Unit Select the desired temperature unit (Celsius, Fahrenheit or Kelvin degrees) and the meter will automatically convert to the selected unit. Manual Temperature If no temperature probe is connected, the desired temperature can be set manually. The default setting is 25.0 °C. If the measured temperature is different, the value can be manually adjusted to obtain an accurate ion measurement.
39

ISE SETUP (HI5222 only)

Temperature Compensation
ISE measurements benefit from temperature compensated corrections if: · standards and sample temperatures differ from each other · the Isopotential Point of the ISE is known. If sample and standards are made at the same temperature, leave this option disabled.

Isopotential Point

If the Temperature Compensation is enabled, the isopoten-

tial point of the ISE must be added in this parameter. Verify

the Electrode Type and Concentration Unit are configured

for the desired application. The Isopotential point will use

the selected concentration unit. Use

and

to edit the isopotential point value and press

to

save the value or press

to cancel operation.

Notes: A warning message will appear on the LCD informing the user to perform a new calibration. A minimum of two ion standards is required for the ISE calibration.

Calibration This option allows the user to view and configure all ISE parameters related to ISE calibration.
Manual Entry Two different standard groups can be used for calibration of ISE: All Standards – During calibration the user can select the desired standards from a large list containing all the predefined standard values and the custom standards. Group Standards – the user can pre-select a group of standards from the existent group of standards to be used during sensor calibration.

40

ISE SETUP (HI5222 only)

To set the Manual Entry:

· Press

while in ISE Measure mode.

· Press

.

· Use

or

to select the Calibration option.

· Press

and use

or

to highlight the Manual Entry option.

· Press

or

to select the desired option.

Edit Custom Standards

Use Edit Custom Standards function to add additional ISE standard values. Up to five custom standard values can be added. Set Electrode Type and Concentration Unit prior to adding these standards.

To edit/set the Custom Standards:

· Press

while in ISE Measure mode.

· Press

.

· Use

or

option.

to select the Calibration

· Press

and use

or

to

highlight the Edit Custom Standards option.

· If you want to disable the custom standard, press

. A pop-up menu will be displayed

asking for confirmation. Press

to confirm (the custom standard value will turn to “—-“)

or press

to cancel the operation.

· Use

key to select the next custom standard to be set.

· Press

to return to Edit Custom Standard options.

Edit Standard Group

If a Group Standard was selected in the parameter Manual Entry, this parameter is used to create your group of standards. If the Standard Group already contains five ISE standards, at least one ISE standard has to be removed in order to add another standard.

To edit/set the Standard Group:

· Press

while in ISE Measure mode.

· Press

.

41

ISE SETUP (HI5222 only)

· Use

or

to select the Calibration option.

· Press

and use

or

the Edit Standard Group option.

to highlight

· Press

and use

and

to choose

the ISE standard to be included in the standard group.

· Press

/

to add/remove the selected ISE

standard to/from the standard group.

· Press

to return to Calibration options and to

save the changes.

Calibration Reminder – See Calibration option from pH

Setup section.

Set Reminder Period – See Calibration option from pH Setup section.

Clear Calibration – See Calibration option from pH Setup section.

Electrode Type
This option allows the user to select the desired Ion Selective Electrode used for measurements from a list: Ammonia, Bromide, Cadmium, Calcium, Carbon Dioxide, Chloride, Cupric, Cyanide, Fluoride, Iodide, Lead, Nitrate, Potassium, Silver, Sodium, Sulfate, Sulfide and five custom ISE. For the standard ISE it is possible to view the Ion constants (Name, Molar Weight and Electric Charge/Slope), while for the custom ISE all these constants can be manually set.

To set the Electrode Type:

· Press

while in ISE Measure mode.

· Press

.

· Use

or

option.

to select the Electrode Type

· Press

and use

or

to select

the desired standard ISE or a custom one from the list.

For standard ISE:

· Press press mode.

to visualize the Ion Constants and then at any time to exit Ion Constants view

42

ISE SETUP (HI5222 only)

· Press

to confirm your selection and return to ISE Setup options.

For custom ISE:

· Press

to edit the Ion Constants for the selected

custom ISE. Use

or

to select the

desired constant and press

to enter edit mode

or

to cancel operation.

· For the ion Name the Text Editor menu will be

displayed on the LCD. Enter the desired information by

accepting the highlighted character which is added in

the text bar, using

. The

and

keys help the user to select the desired character. It is

also possible to delete the last character by positioning

the cursor on the Backspace character ( ) and

pressing

. Press

to return to the

Ion Constants menu.If the Saving Confirmation is

enabled, press

to accept the modified option,

to escape without saving or

to return

to the editing mode. Otherwise, the modified option is

saved automatically.

· To set the appropriate Ion Molar Weight (in g/mol units)

use

or

to set the desired value and

then press

to save the current value or press

to cancel operation.

· To select the appropriate Ion Charge/Slope use

or

and then press

. If the ion electric

charge is None, its slope can be manually set by

pressing

.

A pop-up menu will be displayed on the LCD, in which the slope value can be set using

or

. Press

to save the modified value or press

to return to the previous

menu.

43

ISE SETUP (HI5222 only)

Note: If an ISE calibration was performed and a different Ion Selective Electrode is selected (standard or custom), a warning message appears on the LCD informing the user to perform a new calibration or to select the previous ISE in order to perform accurate measurements.

Concentration Unit

Select the desired concentration unit for the measured ion or chemical compound. The available

concentration units are: ppt, g/L, ppm, mg/L, µg/mL, ppb, µg/L, mg/mL, M, mol/L, mmol/L,

%w/v and User (custom unit).

To set the Concentration Unit:

· Press

while in ISE Measure mode.

· Press

.

· Use

or

to select the Concentration

Unit option.

· Press

and use

or

to

highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

Sample ID – See pH Setup section. Stability Criteria – See pH Setup section. Log – See pH Setup section.
Note: The Logging Data Configuration option includes also the Ion Constants parameter. If you want it to appear in the log reports, it must be enabled.
Alarm – See pH Setup section. Note: The Alarm Limits (Low and High) are set in the selected concentration unit of the measured

44

ISE SETUP (HI5222 only)

ISE Significant Digits

Accessing this option, the number of ISE significant digits can be set, with one (X), two (XX) or three (XXX) significant digits.

To set the ISE Significant Digits:

· Press

while in ISE Measure mode.

· Press

.

· Use

or

Digits option.

to select the ISE Significant

· Press

and use

or

to

highlight the desired option.

· Press

to confirm your selection or press

to cancel operation.

pH CALIBRATION

Calibrate the instrument often, especially if high accuracy is required. The instrument should be recalibrated: · Whenever the pH electrode is replaced. · At least once a week. · After testing aggressive chemicals. · When “Electrode Cond. Unknown”, “pH x Default Calibration” or “pH x Calibration Expired”
message appears on the LCD, in the Reminder messages area. The “x” represents channel “1” or channel “2”.
PREPARATION Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences. For accurate calibration and to minimize cross-contamination, use two beakers for each buffer solution. One for rinsing the electrode and one for calibration.
45

pH CALIBRATION

If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer and pH 4.01, 3.00 or 1.68 as second buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as first buffer and pH 10.01/9.18 or 12.45 as second buffer. For extended range measurements (acidic and alkaline), perform a five points calibration by selecting five of the available buffers.
CALIBRATION PROCEDURE There are 8 standard pH buffers that are temperature- compensated during pH calibrations: pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45. The standard pH buffers are temperature compensated during calibration. Custom buffers require the user to use the actual buffer value at the temperature of use. A minimum of a two point calibration using the standard pH buffers is required to determine the pH electrode condition. The buffers should bracket the sample measurement pH. An extended pH measurement range will require calibration at multiple points. The meter is able of calibration with 5 pH buffers. For improved measurement accuracy, perform a multiple buffer calibration bracketing and including the pH range the sample measurements. The buffer group that will be available during calibration was set in pH setup Calibration Buffer Entry Type. The following example demonstrates pH electrode calibration if Manual selection was selected. In this case all of the 8 standard buffers will be available for calibration.
pH Calibration screen description

· Press

. If the instrument was calibrated before and calibration was not cleared, the old

calibration can be cleared by pressing

. After 10 seconds,

will no longer be

available.

Note: It is very important to clear calibration history when a new electrode is used because

most errors and warning messages that appear during calibration depend on calibration

history.

46

pH CALIBRATION

· Immerse the pH electrode and the temperature probe approximately 4 cm (1.5″) into a buffer

solution of your choice (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 or a custom

buffer) and stir gently. The temperature probe should be close to the pH electrode.

· Select the pH calibration buffer used with

or

. The “Please

wait…” message will appear on the LCD until the reading is stable or the

buffer is validated.

· If the pH buffer is validated,

will appear on the LCD. Press

to update calibration. The calibration buffer will be added to the Calibrated

Buffers section.

· Immerse the pH electrode and the temperature probe into the next buffer solution and follow the

above procedure or press

to exit calibration.

Notes: The new added calibration point will replace

an old one if the difference between them is

±0.2 pH.

If the existing stored calibration is full (five calibration

points), a pop-up menu will be displayed on the LCD

in which you can select with

or

keys the buffer you want to replace with the current

buffer. Press

to delete the selected buffer

and then press

to update calibration with

the new buffer.

· If using manual temperature, after selecting the standard buffer, press

will be displayed on the LCD in which the temperature

value can be adjusted using

or

.

Press

to save the new temperature value.

· If using Custom buffers, press

after buffer

has been accepted to change actual buffer conditions.

A pop-up menu will be displayed on the LCD in which

the custom buffer and the temperature value (MTC)

can be adjusted by pressing

and then

or

keys. Press

to save

the modified value and then

/

to

select next/previous value to be adjusted.

. A pop-up menu

47

pH CALIBRATION

· If the Automatic buffer entry type has been selected for the calibration procedure, the instrument will automatically select the closest buffer to the measured pH value from the edit buffer group (see pH Setup for details).

· If the Semiautomatic buffer entry type has been selected for the calibration procedure, the

instrument will display only the closest buffers to the measured pH value from all the available

buffers and the user must select with

or

the buffer being used.

CALIBRATION MESSAGES

· Move sensor to next buffer or check buffer: this message appears when the difference between the pH reading and the value of the selected calibration buffer is significant. If this message is displayed, check if you have selected the appropriate calibration buffer.

· Wrong buffer temperature: this message appears if the buffer temperature is out of the defined buffer temperature range.

· Clean the electrode or check the buffer. Press

to update calibration: this mes-

sage alerts the user that some dirt or deposits could be on the electrode. Refer to the electrode

Cleaning Procedure.

· Slope too low. Please check the buffer / Slope too high. Please check the buffer: these messages appear if the current slope is under 80 % or over 110 % of default slope. Recalibrate the instrument using fresh buffers.

· Slope too low. Press

to clear old calibration / Slope too high. Press

to

clear old calibration: verify the correct buffer has been selected and poured.

· Unrecognized buffer. Please check the buffer or the buffer list (for Semiautomatic and Automatic buffer entry type): this message appears if the current buffer value is not close to any of the buffers from the buffer list/group. Check if the current buffer is present in the buffer list or the appropriate buffer group was selected.

· The current buffer was already calibrated: change the buffer or press

to exit

calibration mode.

48

pH MEASUREMENT

Verify the pH electrode and instrument has been calibrated before making pH measurements.

DIRECT MEASUREMENT

To measure the pH of a sample using the Direct reading

mode:

· Press

and then

to select pH Mea-

sure mode (select channel first for HI5222).

· Select the Direct reading mode (see pH Setup for

details).

· Place the electrode tip and the temperature probe

approximately 4 cm (1.5″) into the sample to be

tested. Allow time for the electrode to stabilize.

“Stable” will appear.

· The measured pH value will be displayed on the LCD,

together with a short GLP information and display preferences.

Note: If the reading is out of range, “—-” will be displayed on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure pH of a sample using the Direct/AutoHold

reading mode:

· Press

and then

to select pH Mea-

sure mode (for the selected channel – HI5222 only).

· Select the Direct/AutoHold reading mode (see pH

Setup for details).

· Place the electrode tip and the temperature probe approximately 4 cm (1.5″) into the sample to be tested.

· The measured pH value will be displayed on the LCD.

Press

and the “AutoHold” indicator will

start blinking on the LCD until the stability criteria is

reached. The pH value will be frozen on the LCD, along with “AutoHold” indicator.

· To return to normal Measure mode press

.

Note: If the reading is out of range, “—-” will be displayed on the LCD.

49

pH MEASUREMENT

Outside Cal Range warns the user if the current reading is out of the calibrated area. The calibrated area is that part of the pH range in which the calibration point ensures an accurate reading. If the reading is taken out of the calibration area, the “Outside Cal Range”message will start blinking on the LCD. The calibrated area is calculated in accordance with the pH resolution used during the measurement. To avoid triggering this message, the buffer values have to be well-distributed in the desired measurement range.

If measurements are taken successively in different samples, it is recommended to rinse the electrode thoroughly with deionized water or tap water and then with some of the next sample before immersing it into the next sample solution.

The pH reading is affected by temperature. In order to measure the pH accurately, the temperature effect must be compensated. To use the Automatic Temperature Compensation (ATC) feature, connect and place the HI7662-W temperature probe into the sample as close as possible to the electrode and wait for a few seconds.

If the temperature of the sample is known, Manual Temperature Compensation (MTC) can be used by disconnecting the temperature probe.

Notes: For single channel pH measurements (HI5221), “MTC” or “ATC” indicators will be displayed on the LCD, while for dual channel pH measurements (HI5222) “MTC1″/”MTC2” or “ATC1″/”ATC2” indicators will be displayed (Channel dependent).

For mV/Rel mV measurements, “NoProbe” or “TEMP” indicators will be displayed on the LCD (HI5221 only), respectively, “NoProbe 1″/”NoProbe 2” or “TEMP1″/”TEMP2” indicators (HI5222 only) for mV/Rel mV/ISE measurements, depending on the temperature probe status and channel selected.

When in MTC mode, the temperature can be modified by pressing

for pH Measure

mode and

for mV /Rel mV Measure mode (HI5221 only) if the Reading Mode option

is Direct (HI5222 only). The temperature value can be adjusted with

or

from

-20.0 °C to 120.0 °C. Press

to save the new temperature value or press

to return to Measure mode.

When in ATC mode for pH, or TEMP for mV/Rel mV, “—-” will be displayed on the LCD if the measured temperature is under or over temperature range (-20.0 °C to 120.0 °C).

50

mV & Relative mV MEASUREMENTS

mV/ORP MEASUREMENTS
Oxidation-reduction potential (ORP) measurements provide the quantification of the oxidizing or reducing power of the tested sample. To correctly perform a redox measurement, the surface of the ORP electrode must be clean and smooth.

DIRECT MEASUREMENT

To measure the mV of a sample using the Direct reading mode:

· Press

and then

to enter mV Measure

mode (Select channel first for – HI5222 only).

· Select the Direct reading mode (see mV Setup for details).

· Place the tip of the ORP electrode 4 cm (1.5″) into the

sample to be tested and allow a few seconds for the

reading to stabilize.

· The instrument will display the measured mV value on

the LCD.

Note: If the reading is out of range, “—-” will be displayed on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure mV of a sample using the Direct/AutoHold

reading mode:

· Press

and then

to select mV Measure

mode (Select channel first for – HI5222 only).

· Select the Direct/AutoHold reading mode (see mV Setup

for details).

· Place the tip of the ORP electrode approximately 4 cm

(1.5″) into the sample to be tested.

· The measured mV value will be displayed on the LCD.

Press

and the “AutoHold” indicator will start

blinking on the LCD until the stability criteria is reached. The mV value will be frozen on the

LCD, along with “AutoHold” indicator.

· To return to normal Measure mode press

.

Note: If the reading is out of range, “—-” will be displayed on the LCD.

51

mV & Relative mV MEASUREMENTS

Relative mV MEASUREMENTS

To measure the Relative mV of a sample:

· Press

then

HI5222).

(select channel first for

· Verify if a current calibration has been made.

· If required, conduct the single point Rel mV calibration. Verify the tip of the electrode is immersed into the known solution or ORP standard.

· Press

. Use

and

keys

set the standard value. Press

to store the

calibration.

· Press

then

HI5222).

(select channel first for

· Place calibrated sensor tip into the sample to be analyzed. The instrument will display the measured Relative mV value on the LCD, together with a short GLP information about the last calibration or Offset: 0.0 mV no Rel mV calibration was performed.

Notes: If the ORP sensor is not in solution or the measured mV potential is out of range, “—-” will be displayed on the LCD.

52

ISE CALIBRATION (HI5222 only)

For greater accuracy, it is recommended to calibrate the ISE sensors frequently. The instrument should be recalibrated when “ISE x Calibration Expired” (the “x” represents channel “1” or channel “2”) message appears on the LCD, in the Reminder messages area. Due to electrode conditioning time, the electrode must be kept immersed a few seconds to stabilize. The user will be guided step by step during calibration with easy-to-follow messages on the display. This will make the calibration a simple and error-free procedure. PREPARATION Pour small quantities of the standard solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences. For accurate calibration and to minimize cross-contamination, use two beakers for each standard solution. One for rinsing the electrode and one for calibration.
Note: To read concentration (not activity) ISA must be added to the standards and samples. No corrections are needed due to dilutions. CALIBRATION PROCEDURE The ISE calibration and measurement can be performed with or without temperature compensation. If the temperature compensation option is enabled, the isopotential point of the electrode must be set in ISE Setup in order to perform correct concentration measurements. Before calibrating, make sure that the appropriate Electrode Type has been selected in ISE Setup according to the measured Ion/compound. ISE Calibration screen description
53

ISE CALIBRATION (HI5222 only)
2 cm (1″)

The group of calibration standards is set under ISE Setup Calibration. Select standards that are in the measurement range of the samples.

To calibrate the instrument:

· Press

. If the instrument was calibrated before and calibration was not cleared, the old

calibration can be cleared by pressing

. After 10 seconds,

will no longer be

available.

Note: It is very important to clear calibration history when a new electrode is used because most errors and warning messages that appear during calibration depend on calibration history.

· Add ISA to both standard solutions and samples.

· Immerse the Ion Selective Electrode and the temperature probe approximately 2 cm (1″) into the less concentrated standard solution and stir gently.

· Select the appropriate standard solution concentration with

or

. For All Standards manual entry mode, the standard

concentration can be selected from a list containing all the predefined and

custom standards. For Group Standard manual entry mode the standard

concentration can be selected from the predefined group of standards.

Press

to calibrate the electrode in the standard.

Note: To adjust standard value: Press

. A pop-up menu will be displayed on the LCD

in which the concentration value can be adjusted using

or

. Press

to save the new concentration value.

· The “Please wait…” message will appear on the LCD for 10 seconds. Remove ISE from first

standard, rinse tip and immerse the Ion selective electrode and the temperature probe into the

next standard solution and follow the above procedure or press

to exit calibration.

Notes: The new added calibration point will replace

an old one if the difference between them is less than

20 % of the standard solution.

If the existing stored calibration is full (five calibration

points), a pop-up menu will be displayed on the LCD

in which you can select with

or

the standard solution you want to replace with the

current one.

54

ISE CALIBRATION (HI5222 only)

Press

to delete the selected calibrated point and then press

calibration with the new standard solution.

If the isopotential point of the electrode is unknown, the ISE calibration and measurements can be performed without temperature compensation (see ISE Setup, Temperature option for details).

When in MTC mode, after selecting a standard press

, a pop-up menu will be displayed on the LCD in which the concentration and the temperature

value can be adjusted by pressing

and then

or

keys. Press

to save

the modified value and then

/

to select next/previous value to be adjusted. MTC

value will have no effect on measurement but will

be included on log data.

to update

CALIBRATION MESSAGES

· Wrong standard solution. Check the standard solution: this message appears when the difference between the reading and the value of the selected standard solution concentration is significant. If this message is displayed, check if you have selected the appropriate calibration standard.

· Standard to close. Check the standard or clear calibration: this message appears when the difference between current ISE standard and the already calibrated standard is too low.

· Slope to low/high. Check the standard solution. Recalibrate using fresh standards.

· Difference between standards temperature is too high. Press or clear old calibration.

to update calibration

55

ISE MEASUREMENT (HI5222 only)

Make sure the instrument and ISE sensor have been calibrated before making ISE measurements.
When using one of the incremental methods for measurement, at least a two point ISE calibration must be performed to establish the electrode slope. For accurate measurements, add the appropriate ISA (Ionic Strength Adjuster) to both samples and standards. Consult ISE manual for sensor preparation details.

DIRECT MEASUREMENT

To measure the concentration of a sample using the Direct reading mode:

· Press

and then

to select ISE Measure

mode for the selected channel.

· Select the Direct reading mode (see ISE Setup for details).

· Add ISA to the sample solution.

· Submerge the Ion Selective Electrode tip and the temperature probe approximately 2 cm (1″) into the sample. Allow time for the electrode to stabilize.

· The measured concentration value will be displayed on the LCD in the selected units.

Note: If the reading is out of range, “—-” will be displayed on the LCD.

DIRECT/AUTOHOLD MEASUREMENT

To measure the concentration of a sample using the

Direct/AutoHold reading mode:

· Press

and then

to select ISE Measure

mode for the selected channel.

· Select the Direct/AutoHold reading mode (see ISE Setup

for details).

· Add ISA to the sample solution.

· Dip the Ion Selective Electrode tip and the temperature

probe approximately 2 cm (1″) into the sample to be

tested.

· The measured concentration value will be displayed on the LCD. Press

, the “AutoHold”

indicator will blink on the LCD until the stability criterion is reached. The concentration value will

be frozen on the LCD, along with “AutoHold” indicator.

56

ISE MEASUREMENT (HI5222 only)

· To return to normal Measure mode press

.

Note: If the reading is out of range, “—-” will be displayed on the LCD.

KNOWN ADDITION

To measure the concentration of a sample using the Known Addition incremental method:

· Press

and then

to select ISE

Measure mode for the selected channel.

· Select the Known Addition method (see ISE Setup for details).

· Prior to starting a KA procedure, the ISE sensor must be calibrated with a minimum of two standards containing ISA. The slope of the electrode will be used in all calculations involved in KA.

· If following an established procedure: Press

,

Edit the method variables and follow the procedure

below.

· Press

to set the method parameters. Press

/

to select next/previous parameter

to edit, then press

and use

or

to set the desired parameter value. Press

to save the modified value and then press

to exit method parameters edit menu.

· If developing a procedure: Before attempting Known Addition analysis it is important to determine what sample volume, standard concentration and standard volume will produce the best results. As a general rule, the addition of standard should change the mV value of the sample by 15 – 20 mV. For a positively charged ion (i.e. Sodium, Potassium, Calcium), the standard addition should increase the mV. For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the standard addition should decrease the mV. Start with a small trial. For example: Measure 50 mL of sample, add a magnetic stir bar, place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the highest ISE standard available (i.e. 0.1M or 1000 ppm).

57

ISE MEASUREMENT (HI5222 only)

Start by adding 500 µL at a time (for example). Watch the change in mV. When you have

observed approximately a 15 mV change from the original sample, calculate the total volume

added. Adjust sample and standard volumes proportionally to standard volumes that can be

measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.

· Press

: Edit the procedure variables to the volumes determined in the prior step.

Procedure:

· Press

to enter Known Addition mode.

· Volumetrically add sample to a clean beaker. Add a magnetic stir bar and place on a stir plate.

Stir sample. The method will prompt user to add ISA. Place ISE sensor tip into the solution and

a mV value will show on the display.

· Press

to take the first mV reading.

· When the reading is stable, press

to store the first mV reading. The second step of

the method will be displayed on the LCD in which the user is notified to add the Volume of

Standard to the sample.

· Press

to take the second mV reading.

· When the reading is stable, press

to store

the second mV reading. The ISE measurement results

will be displayed on the LCD.

· Press

to log the current results into a ISE

Method Report. Press

to return to ISE

Measure mode. Press

to measure additional

samples. Rinse ISE sample between samples.

· Press

to modify the method parameters.

Note: Press

at any time to stop the

measurement and return to ISE Measure mode.

KNOWN SUBTRACTION

To measure the concentration of a sample using the Known Subtraction method:

· Press

and then

to select ISE Measure mode for the selected channel.

· Select the Known Subtraction method (see ISE Setup Reading Mode).

· Prior to starting a KS procedure, the ISE sensor must be calibrated with a minimum of two

standards containing ISA. The slope of the electrode will be used in all calculations involved in

KS.

58

ISE MEASUREMENT (HI5222 only)

· If following an established procedure: Press

then edit the method variables and follow

the procedure below.

· Press

to set the method parameters. Press

/

to select next/previ-

ous parameter to edit, then press

and use

or

to set the desired

parameter value. Press

to save the modified value and then press

to exit

method parameters edit menu.

· If developing a procedure: Before attempting Known Subtraction analysis it is important to

determine what sample volume, standard reactant concentration and standard volume will

produce the best results and the way the reagent will react with the measured ion on a molar

basis (stoichiometric factor). As a general rule, the addition of standard should change the mV

value of the sample by 15-20 mV.

For a positively charged ion (i.e. Calcium), the reactant addition should decrease the mV. For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the reactant addition should increase the mV. Start with a small trial. For example: Measure 50 mL of sample, add a magnetic stir bar, place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip in to the sample. Put instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the reactant standard. Start by adding 500 µL at a time (for example). Watch the change in mV. When you have observed approximately a 15 mV change from the original sample, calculate the total volume added. Adjust sample and standard volumes proportionally to standard volumes that can be measured with accuracy. Use volumetric pipettes for standard, ISA and reagent addition.

· Press

then edit the procedure variables to the volumes determined in the prior step.

Procedure:

· Press

to enter Known Subtraction mode.

· Volumetrically add sample to a clean beaker. Add a magnetic stir bar and place on a stir plate. Stir sample. The method will prompt user to add ISA. Place ISE sensor tip into the solution and a mV value will show on the display.

· Press

to take the first mV reading.

· When the reading is stable, press

to store the first mV reading. The second step of the

method will be displayed on the LCD in which the user is notified to add the volume of reagent

to the sample.

· Press

to take the second mV reading.

59

ISE MEASUREMENT (HI5222 only)

· When the reading is stable, press

to store the second mV reading. The ISE measurement

results will be displayed on the LCD.

· Press

to log the current results into an ISE Method Report. Press

to return to

ISE Measure mode. Press

to start another measurement. Rinse ISE sensor between

samples.

· Press

, to modify parameters.

Note: Press

at any time to stop the measurement and return to ISE Measure mode.

ANALYTE ADDITION

To measure the concentration of a sample using Analyte Addition method:

· Press

and then

to select ISE Measure mode for the selected channel.

· Select the Analyte Addition method (see ISE Setup Reading Mode).

· Prior to starting an AA procedure, the ISE sensor must be calibrated with a minimum of two

standards containing ISA. The slope of the electrode will be used in all calculations involved in

AA.

· If following an established procedure: Press

then edit the method variables and follow

the procedure below.

· Press

to set the method parameters. Press

/

to select next/previ-

ous parameter to edit, then press

and use

or

to set the desired

parameter value. Press

to save the modified value and then press

to exit

method parameters edit menu.

· If developing a procedure: Before attempting Analyte Addition analysis, it is important to

determine which standard volume, concentration and sample size will produce the best results.

As a general rule, the standard must be less concentrated than the sample so the addition of

sample will increase the total ion content of the solution from the beaker and change the mV

value by at least 10 mV. For a positively charged ion (i.e. Sodium), the AA increases the mV.

For a negatively charged ion (i.e. Sulfide, Fluoride, Chloride), the AA should decrease the mV.

Start with a small trial. For example: Measure 50 mL of standard, add a magnetic stir bar and

place on a stirrer, add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put

instrument in mV mode and record the observed mV. Using a micropipette, add a volume of the

sample. Start by adding 500 µL at a time (for example). Watch the change in mV. When you

have observed approximately a 10 mV change from the original standard, calculate the total

volume added. Adjust sample and standard volumes proportionally to standard volumes that

can be measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.

60

ISE MEASUREMENT (HI5222 only)

· Press

then edit the procedure variables to the volumes determined in the prior step.

Procedure:

· Press

to enter Analyte Addition mode.

· Volumetrically add standard to a clean beaker. Add a magnetic stir bar and place on a stir plate.

Stir standard. The method will prompt user to add ISA. Place ISE sensor tip into the solution

and a mV value will show on the display.

· Press

to take the first mV reading.

· When the reading is stable, press

to store the first mV reading. The second step of the

method will be displayed on the LCD, in which the user is notified to add the Sample Volume

to the standard solution. The method parameters are also displayed on the LCD.

· Press

to take the second mV reading.

· When the reading is stable, press

to store the second mV reading. The ISE measurement

results will be displayed on the LCD.

· Press

to log the current results into an ISE Method Report. Press

to return to

ISE Measure mode.

· Press

to start another measurement. Rinse ISE sensor between samples.

· Press

, to modify the method parameters.

Note: Press

at any time to stop the measurement and return to ISE Measure mode.

ANALYTE SUBTRACTION

To measure the concentration of a sample using Analyte Subtraction method:

· Press

and then

to select ISE Measure mode for the selected channel.

· Select the Analyte Subtraction method (see ISE Setup Reading Mode).

· Prior to starting an AS procedure, the ISE sensor must be calibrated with a minimum of two standards containing ISA. The slope of the electrode will be used in all calculations involved in AS.

· If following an established procedure: Press the procedure below.

then edit the method variables and follow

· Press

to set the method parameters. Press

/

to select next/previ-

ous parameter to edit, then press

and use

or

to set the desired

parameter value. Press

to save the modified value and then press

to exit

method parameters edit menu.

61

ISE MEASUREMENT (HI5222 only)

· If developing a procedure: Before attempting Analyte Subtraction analysis, it is important to

determine which sample volume, reactant volume and concentration, will produce the best results

and the way the reagent will react with the measured ion on a molar basis (stoichiometric fac-

tor). As a general rule, the reactant should contain the measured ion so the sample addition

will react with the ion and reduce the measured concentration of the sample. The change of the

mV value, before and after the sample addition, should be at least 10 mV. Start with a small

trial. For example: Measure 50 mL of reactant, add a magnetic stir bar and place on a stirrer,

add ISA (consult ISE manual) and place ISE electrode tip into the sample. Put instrument in

mV mode and record the observed mV. Using a micropipette, add a volume of the sample.

Start by adding 500 µL at a time (for example). Watch the change in mV. When you have

observed approximately a 10 mV change from the original value, calculate the total volume

added. Adjust sample and standard volumes proportionally to standard volumes that can be

measured with accuracy. Use volumetric pipettes for standard, ISA and sample addition.

· Press

then edit the procedure variables to the volumes determined in the prior step.

Procedure:

· Press

to enter Analyte Subtraction mode.

· Volumetrically add reactant to a clean beaker. Add a magnetic stir bar and place on a stir plate. Stir standard. The method will prompt user to add ISA. Place ISE sensor tip into the solution and a mV value will show on the display.

· Press

to take the first mV reading.

· When the reading is stable, press

to store the first mV reading. The second step of the

method will be displayed on the LCD in which the user is notified to add the Sample Volume

to the standard solution.

· Press

to take the second mV reading.

· When the reading is stable, press

to store the second mV reading. The ISE measurement

results will be displayed on the LCD.

· Press

to log the current results into an ISE Method Report. Press

to return

to ISE Measure mode. Press

to start another measurement. Rinse sensor between

samples.

· Press

to modify the method parameters.

Note: Press

at any time to stop the measurement and return to ISE Measure mode.

62

TEMPERATURE CALIBRATION

The user temperature calibration menu can be accessed during meter startup by simultaneously pressing three keys as shown in the drawing below. Press the keys after the short beep is heard at the meter power on. Keep all three keys pressed until Temp. Calibration menu appears.

Note: The user temperature calibration is performed at three points: around 0 °C, 50 °C and 100 °C.

To perform the user temperature calibration:

· Select the desired temperature channel by pressing

(HI5222 only).

· Press

to start the temperature calibration.

Adjust the temperature preset value using

or

when necessary.

· Insert the temperature probe into the beaker with water

at 0 °C.

· Wait for measurement to stabilize and then press

to confirm the calibration point.

· Repeat the previous steps for 50 °C and 100 °C.

· Save the calibration.

· Press

to return to measure mode.

Note: Press

if you want to clear the temperature user calibration.

63

LOGGING

There are 5 ways the Reading Mode and Log may be configured together. The table below shows the combinations and indicates where the completed log will be stored.

Reading Mode

Log Automatic (1)

Direct

Manual (2)

Auto Hold (NA)

Direct/AutoHold

Automatic (3) Manual (4) Auto Hold (5)

log Recall Automatic Log Manual Log Not Applicable Automatic Log Manual Log Manual Log

1. Direct Reading Mode and Automatic Log:

Real time continuous measurements are on display with

continuous logging to meter memory. Press

.

64

LOGGING

2. Direct Reading Mode and Manual Log:

Real time continuous measurements are on display and

snapshots of measurement data are stored in the Manual

log when the user presses

. Subsequent snapshots

will be added to the same Manual Lot every time the

is depressed unless New Lot is selected under

Log options.

Note: When the

is pressed the lot ID along

with the current record number will appear for

short time on the selected channel window on the

top/left corner (e.g. L033_MV 8 – this means lot

ID L033_mV and record number 8).

3. Direct/AutoHold Reading Mode and Automatic Log

Press

and then

keys must be pressed

on front display to initiate this function. Real time continuous

measurements are on display with “AutoHold” flashing

and real time continuous logging into meter memory,

until the meter reaches the stability criteria to go into

AutoHold mode. The stored sample logs will be marked

with an “H” to indicate the AutoHold mode. The virtual

key

returns operation to real time continuous

measurements and

stops the logging session.

4. Direct/AutoHold Reading Mode and Manual Log

Press

in order to add one new record in the log report. The manual log is working even

if it is in AutoHold or Continuous Reading mode. Press

to initiate the AutoHold event.

“AutoHold” will flash until the stability criteria is reached and then the screen freezes in Auto

Hold mode, the data is marked with an “H”.

65

LOGGING

5. Direct/AutoHold Reading Mode and Auto Hold Log

Press

and then

keys initiate and automate the capture of stable data which is

stored in the Recall Manual Log file. During the process, “AutoHold” will flash until the stability

criteria is reached and then the screen freezes in AutoHold mode, the data is logged and marked

with an “H”. The virtual key

returns operation to Real time continuous measurement.

Press

again to log a second stable data point. The lot ID along with the record index will

appear for short time on the top/left corner on the selected channel window, every time a record

will be added to the lot.

LOG RECALL

This feature allows the user to view all stored data. If

no data was logged, the “No records were found”

message will be displayed on the LCD in the Log Recall

screen. Otherwise, the instrument will display all the

memorized lots in accordance with the selected option:

Automatic Log, Manual Log or ISE Method Report

(HI5222 only).

To view the memorized data:

· Press

while in Measure mode.

· Press

. The “Choose Log Report Type”

message will be displayed in the Reminder

messages area.

· Press

,

or

to select the

desired Log Report type. All logged lots for the selected

Log Report type will be displayed on the LCD.

· To filter the displayed lots, press

and then

the desired parameter

,

or

-HI5222 only. Only the selected measurement

parameter lots will be displayed on the LCD.

66

LOGGING

· Select the desired lot with

or

and

press

to display the logged data from the

highlighted lot. The “Please wait…” message will

be displayed on the LCD for one second. The selected

Logging Data Configuration options will be displayed

on the LCD, together with GLP information (last

calibration date and calibrated buffers/standards)

if a calibration has been performed on the selected

mode and the logged values (measured value, mV

value, temperature value, temperature compensation

mode and the logging time).

Note: For automatic logging only, it is possible to view the plotted graph.

Press

to display the graph.

By pressing

it is possible to move the

graph along the X or Y axis with the arrow keys.If

pressing

while the graph is displayed, the

zoom menu for the X and Y axes will be accessed.

Press

or

/

/

/

to switch between the

active zooming axes and then zoom in or out

on the selected axis by pressing the appropriate

virtual key.

· Press

to return to the previous menu at

any time.

67

LOGGING

To delete lots:

· Press

while in Log Recall mode.

· Press

or

to access delete or delete

all mode. Otherwise, press

to return to Log

Recall view mode.

· After selecting one of the deleting modes, use

or

to select one lot and then press

or

to delete the selected lot or all lots. The

“Please wait…” message will be displayed on the

LCD until the selected lot or all lots are deleted.

· Press

and then press

to exit deleting

mode and return to Log Recall view mode.

· Press

to exit Log Recall mode and return

to Measure mode.

Note: Logged lots should also be deleted whenever “Please Delete Old Log Files” or “Low Data

Logging Space” message appears on the LCD, in the Reminder messages area.

Data transmission from the instrument to the PC can be done with the HI92000 Windows® compatible software (optional). HI92000 also offers graphing and on- line help features. Data logged on the HI5221 and HI5222 meters can be exported to the most popular spreadsheet programs for further analysis. HI5221 and HI5222 instruments have an USB interface available.
Use a standard USB cable to connect your instrument to the PC. Make sure that the instrument and the HI92000 software have the same baud rate and the appropriate communication port. The PC software may also be used for real time logging. To allow our users access to the latest version of Hanna Instruments PC compatible software, we made the products available for download at http://software.hannainst.com. Select the product code and click Download Now. After download is complete, use the setup.exe file to install the software.

PC INTERFACE

68

ADDITIONAL INFORMATION

ISE THEORY
An Ion Selective Electrode (ISE) is an electrochemical sensor that changes voltage with the activity or concentration of ions in solutions. The change in voltage is a logarithmic relationship with concentration, and is expressed by the Nernst equation:

E = Eo + S log(a)

where: E – the measured voltage; Eo – standard voltage and other standard system voltages; a – the activity of the ion being measured;

S

=

2.303RT nF

S – the Nernst slope factor and is derived from thermodynamic principles: R – the universal gas constant (8.314 J/(Kmol)); T – the temperature in degrees Kelvin; F – the Faraday’s constant (96,485 C/mol); n – the ion charge. The slope may be positive or negative depending upon the Ion charge (n).

SPECIES
Monovalent cation Monovalent anion Divalent cation Divalent anion

SLOPE (mV/decade)
+59.16 ­59.16 +29.58 ­29.58

Activity and concentration are related by an “activity coefficient”, expressed as:
a = · C
where: a – the activity of the ion being measured; – the activity coefficient; C – the concentration of the ion being measured.

69

ADDITIONAL INFORMATION

In very dilute solutions approaches 1 so activity and concentration are the same. Actual samples that are more concentrated have much smaller activity coefficients ( < 1). The addition of an inert background salt to standards and samples stabilizes the activity coefficient so that concentration measurements may be made directly. Some of Hanna Instruments’s Ionic Strength Adjuster formulations also may optimize pH and complex interferences, in addition to standardizing the ionic strength.
The Nernst equation can be written: E = Eo + S log(C)
ION SELECTIVE ANALYSIS METHODS

Direct Analysis
This method is a simple procedure for measuring multiple samples. It should only be used in the linear working regions of the sensor. A direct reading instrument such as the HI5222 determines concentration of the unknown by a direct reading after calibrating the instrument with the standards. The instrument is calibrated as described in “ISE CALIBRATION & MEASUREMENTS” section, with two or more freshly made standards that are in the measurement range of the unknowns. Ionic strength adjustment is made to samples and standards. Unknowns are measured directly by the instrument. At lower concentrations, in non-linear regions of the electrode response, multiple calibration points will extend measurements to a practical detection limit. Calibrations must be performed more frequently in these cases.

Incremental Methods

Incremental methods are useful for the measurement of samples whose constituents are variable or concentrated. Incremental techniques can reduce errors from such variables as temperature, viscosity, or pH extremes and will provide indirect analysis of ions for which there is no ISE sensor for a direct measurement. There are four commonly used different incremental methods for sample measurement. They are Known Addition, Known Subtraction, Analyte Addition and Analyte Subtraction. HI5222 allows the analyst to use these techniques as a simple routine procedure, thus eliminating calculations or tables. The method once set up can be used for repetitive measurements on multiple samples. Known Addition and Known Subtraction

With Known addition, standard is added to a sample being measured. The standard and sample contain the same ion. mV are taken before and after the standard addition. From the change in mV, the sample concentration is determined.

C ·V

(V + V )

CSAMP = (V

STD STD
E
+ V + V )·10 S- (V

· SAMP

ISA

+V ) V

SAMP

STD

ISA

SAMP

ISA

SAMP

70

ADDITIONAL INFORMATION

With Known subtraction, a known standard is added to an ionic sample being measured. The standard reacts with the measured ion in the sample in a known manner, thus removing measured ions from the solution. From the change in mV, the concentration of the sample is determined.

C ·V ·f

(V + V )

CSAMP

=

(VSAMP

VISA)

–

STD STD
(VSAMP +

VSTD

·E VISA)·10 S

SAMP

ISA

VSAMP

where: CSAMP – the sample concentration; CSTD – the standard concentration; VSAMP – the sample volume;

E – the difference of potential from the electrode;
S – the electrode slope, determined in a previous calibration;

VSTD – the standard volume;

f – the stoichiometric ratio between sample

VISA – ISA volume

and standard;

Example 1

You have sulfide samples and you are adding Ag+. The reaction is:

S2- + 2Ag+ Ag2S One mole sulfide sample reacts with 2 moles silver standard (f = 1/2).

Example 2

You have sulfide samples and you are adding Pb2+. The reaction is:

S2- + Pb2+ PbS One mole sulfide sample reacts with 1 mole lead standard (f = 1).

Analyte Addition and Analyte Subtraction

Analyte Addition and Subtraction are variations of the previous two methods.

With Analyte Addition, sample (analyte) is added to an ion standard being measured. The

standard and sample contain the same ion. mV are taken before and after the sample addition.

From the mV the analyte concentration is determined.

E

C · V (V + V + V )·10

CSAMP

=

STD
(VSTD

VSTIDSA·)

STD

SAMP

ISA

VSAMP

–

(SVSTD

V) ISA

With Analyte Subtraction, sample (analyte) is added to an ion standard being measured. The

analyte reacts with the measured ion in a known manner thus removing measured ions from the

solution. From the change in mV the concentration of the analyte is determined.

{ [ ] } ( ) C =f · SAMP

(VSTD + VISA) V

1 + (VSTD + VISA)

E
·10 S

V

· CSTD·VSTD V +V

SAMP

SAMP

STD

ISA

71

where: CSAMP – the sample concentration; E- the difference of potential from the electrode;

C STD

– the standard concentration; S

– the electrode slope, determined in a previous

VSAMP – the sample volume; VSTD – the standard volume; V – ISA volume;

calibration;
f – the stoichiometric ratio between sample and standard;

ISA

pH BUFFER TEMPERATURE DEPENDENCE

Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lower degree than normal solutions.
During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature.

TEMP

pH BUFFERS

°C

K °F 1.679 3.000 4.010 6.862 7.010 9.177 10.010 12.454

0 273 32 1.670 3.072 4.007 6.982 7.130 9.459 10.316 13.379

5 278 41 1.670 3.051 4.002 6.949 7.098 9.391 10.245 13.178

10 283 50 1.671 3.033 4.000 6.921 7.070 9.328 10.180 12.985

15 288 59 1.673 3.019 4.001 6.897 7.046 9.273 10.118 12.799

20 293 68 1.675 3.008 4.004 6.878 7.027 9.222 10.062 12.621

25 298 77 1.679 3.000 4.010 6.862 7.010 9.177 10.010 12.450

30 303 86 1.683 2.995 4.017 6.851 6.998 9.137 9.962 12.286

35 308 95 1.688 2.991 4.026 6.842 6.989 9.108 9.919 12.128

40 313 104 1.693 2.990 4.037 6.837 6.983 9.069 9.881 11.978

45 318 113 1.700 2.990 4.049 6.834 6.979 9.040 9.847 11.834

50 323 122 1.707 2.991 4.062 6.834 6.978 9.014 9.817 11.697

55 328 131 1.715 2.993 4.076 6.836 6.979 8.990 9.793 11.566

60 333 140 1.724 2.995 4.091 6.839 6.982 8.969 9.773 11.442

65 338 149 1.734 2.998 4.107 6.844 6.987 8.948 9.757 11.323

70 343 158 1.744 3.000 4.123 6.850 6.993 8.929 9.746 11.211

75 348 167 1.755 3.002 4.139 6.857 7.001 8.910 9.740 11.104

80 353 176 1.767 3.003 4.156 6.865 7.010 8.891 9.738 11.003

85 358 185 1.780 3.002 4.172 6.873 7.019 8.871 9.740 10.908

90 363 194 1.793 3.000 4.187 6.880 7.029 8.851 9.748 10.819

95 368 203 1.807 2.996 4.202 6.888 7.040 8.829 9.759 10.734

During calibration the instrument will display the pH buffer value at 25 °C.

72

ELECTRODE CONDITIONING AND MAINTENANCE

PREPARATION PROCEDURE Remove the protective cap off the pH electrode. SALT DEPOSITS MAY BE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode. These bubbles can be removed by “shaking down” the electrode as you would do with a glass thermometer. If the bulb and/or junction is dry, soak the electrode in HI70300 or HI80300 Storage Solution for at least one hour.
For refillable electrodes: If the filling solution (electrolyte) is more than 2 cm (1″) below the fill hole, add HI7082 or HI8082 3.5M KCl Electrolyte Solution for double junction or HI7071 or HI8071 3.5M KCl+AgCl Electrolyte Solution for single junction electrodes. Unscrew the fill hole screw during measurements. This will allow electrolyte to flow out of the junction.
73

ELECTRODE CONDITIONING AND MAINTENANCE

For AmpHel® electrodes:
If the electrode does not respond to pH changes, the battery may have run down and the electrode or battery (if replaceable) should be replaced.
MEASURE Rinse the pH electrode tip with distilled water. Immerse the tip bottom 4 cm (1.5″) in the sample and stir gently for a few seconds. For a faster response and to avoid cross-contamination of the samples, rinse the electrode tip with a few drops of the solution to be tested, before taking measurements.
STORAGE PROCEDURE To minimize clogging and ensure a quick response time, the glass bulb and the junction of pH electrode should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI70300 or HI80300 Storage Solution or, in its absence, Filling Solution (HI7071 or HI8071 for single junction and HI7082 or HI8082 for double junction electrodes). Follow the Preparation Procedure before taking measurements.
Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.
PERIODIC MAINTENANCE Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse off any salt deposits with water.
pH PROBE MAINTENANCE
For refillable electrodes: Refill the reference chamber with fresh electrolyte (HI7071 or HI8071 for single junction or HI7082 or HI8082 for double junction electrodes). Allow the electrode to stand upright for 1 hour. Follow the Storage Procedure above.
pH CLEANING PROCEDURE
· General Soak in Hanna Instruments HI7061 or HI8061 General Cleaning Solution for approximately 30 minutes.
· Protein Soak in Hanna Instruments HI7073 Protein Cleaning Solution for 15 minutes. · Inorganic Soak in Hanna Instruments HI7074 Inorganic Cleaning Solution for 15 minutes.
This is good at cleaning a black ceramic junction. · Oil/grease Rinse with Hanna Instruments HI7077 or HI8077 Oil and Fat Cleaning Solution.
74

IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled electrodes) and soak the electrode in HI70300 or HI80300 Storage Solution for at least 1 hour before taking measurements.

TROUBLESHOOTING GUIDE

SYMPTOMS Slow response/excessive drift
Readings fluctuate up and down (noise).
The LCD displays “_” during measurements (pH, mV, mV Rel or ISE).
Out of range in the mV scale.
The instrument does not work with the temperature probe. The meter fails to calibrate or gives faulty readings. Explicit warnings are displayed during calibration. The electrode condition is not displayed after calibration. The instrument does not start up.

PROBLEM

SOLUTION

Dirty pH electrode. Dirty reference junction.

Soak the electrode tip in HI7061 solution for 30 min. and then clean the electrode. Soak in HI7074.

Clogged/dirty junction. Low electrolyte level (refillable electrodes only). Out of range in the appropriate scale.
Dry reference junction. Out order temperature probe.

Clean the electrode. Refill with fresh solution (for refillable electrodes only).
Check to see if the pH bulb and reference junction are in the solution. Make sure the sample is in the specified range.Check the electrolyte level and the general state of the pH/ORP or ISE electrode.
Soak in HI70300 Storage solution for at least one hour.
Replace the probe.

Broken or out of order electrode.
Dirty/broken electrode, contaminated buffers.
Only one-point calibration has been performed.
Internal or software error.

Replace the electrode.
Follow displayed instructions.
Perform at least a two-point calibration. Restart the instrument using the power switch or unplug adapter.If the error persists, contact your local Hanna Instruments Office.

75

TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS

The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher.

Since the resistance of the pH electrode is in the range of 50 ­ 200 M, the current across the membrane is in the pico Ampere range. Large currents can disturb the calibration of the electrode for many hours.
The pH electrode’s life also depends on the temperature. If constantly used at high temperatures, the electrode life is drastically reduced.

Typical Electrode Life Ambient Temperature 90 °C (194 °F) 120 °C (248 °F)

1 ­ 3 years Less than 4 months Less than 1 month

Alkaline Error
High concentrations of sodium ions interfere with readings in alkaline solutions. The pH at which the interference starts to be significant depends upon the composition of the glass. This interference is called alkaline error and causes the pH to be underestimated.

76

ACCESSORIES

pH BUFFER SOLUTIONS

HI6016

pH 1.679 Buffer Solution, 500 mL bottle

HI6003

pH 3.000 Buffer Solution, 500 mL bottle

HI8004L

pH 4.01 Buffer Solution in FDA approved bottle, 500 mL

HI6004

pH 4.010 Buffer Solution, 500 mL bottle

HI8006L

pH 6.86 Buffer Solution in FDA approved bottle, 500 mL

HI6068

pH 6.862 Buffer Solution, 500 mL bottle

HI8007L

pH 7.01 Buffer Solution in FDA approved bottle, 500 mL

HI6007

pH 7.010 Buffer Solution, 500 mL bottle

HI8009L

pH 9.18 Buffer Solution in FDA approved bottle, 500 mL

HI8010L

pH 10.01 Buffer Solution in FDA approved bottle, 500 mL

HI6010

pH 10.010 Buffer Solution, 500 mL bottle

HI6124

pH 12.450 Buffer Solution, 500 mL bottle

ELECTRODE STORAGE SOLUTIONS

HI70300L

Storage Solution, 500 mL bottle

HI80300L

Storage Solution in FDA approved bottle, 500 mL

ELECTRODE CLEANING SOLUTIONS

HI70000P

Electrode Rinse Sachets, 20 mL, 25 pcs

HI7061L

General Purpose Solution, 500 mL bottle

HI7073L

Protein Cleaning Solution, 500 mL bottle

HI7074L

Protein Cleaning Solution, 500 mL bottle

HI7077L

Oil & Fat Cleaning Solution, 500 mL bottle

HI8061L

General Purpose Solution in FDA approved bottle, 500 mL

HI8073L

Protein Cleaning Solution in FDA approved bottle, 500 mL

HI8077L

Oil & Fat Cleaning Solution in FDA approved bottle, 500 mL

ELECTRODE REFILL ELECTROLYTE SOLUTIONS

HI7071

3.5M KCl + AgCl Electrolyte, 4×30 mL, for single junction electrodes

HI7072 HI7082

1M KNO3 Electrolyte, 4×30 mL 3.5M KCl Electrolyte, 4×30 mL, for double junction electrodes

77

ACCESSORIES

HI8071

3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4×30 mL, for single junction electrodes

HI8072 HI8082

1M KNO3 Electrolyte in FDA approved bottle, 4×30 mL 3.5M KCl Electrolyte in FDA approved bottle, 4×30 mL, for double junction electrodes

HI8093

1M KCl + AgCl Electrolyte in FDA approved bottle, 4×30 mL

ORP SOLUTIONS

HI7021L

Test Solution 240 mV, 500 mL bottle

HI7022L

Test Solution 470 mV, 500 mL bottle

HI7091L

Reducing Pretreatment Solution

HI7092L

Oxidizing Pretreatment Solution, 500 mL

pH ELECTRODES
All electrodes part numbers ending in B are supplied with a BNC connector and 1 m (3.3′) cable, as shown below:

HI1043B Glass body, double junction, refillable, combination pH electrode. Use: strong acid/alkali.

HI1053B Glass body, triple ceramic, conical shape, refillable, combination pH electrode. Use: emulsions.
78

ACCESSORIES

HI1083B Glass body, micro, Viscolene, non refillable, combination pH electrode. Use: biotechnology, micro titration.
HI1131B Glass body, refillable, double junction, combination pH electrode. Use: general purpose.
HI1330B Glass body, semimicro, single junction, refillable, combination pH electrode. Use: laboratory, vials.
HI1331B Glass body, semimicro, single junction, refillable, combination pH electrode. Use: flasks.
HI1230B Plastic body (PEI), double junction, gel filled, combination pH electrode. Use: general, field.
79

ACCESSORIES

HI2031B Glass body, semimicro, conical, single junction, refillable, combination pH electrode. Use: semisolid products.
HI1332B Plastic body (PEI), double junction, refillable, combination pH electrode. Use: general purpose.
HI1413B Glass body, single junction, flat tip, Viscolene, non refillable, combination pH electrode. Use: surface measurement.
FC100B Plastic body (PVDF), double junction, refillable, combination pH electrode. Use: general purpose for food industry.
FC200B Plastic body (PVDF), single junction, conical, Viscolene, non refillable, combination pH electrode. Use: meat & cheese.
80

ACCESSORIES

FC210B Glass body, double junction, conical, Viscolene, non refillable, combination pH electrode. Use: milk, yogurt.
FC220B Glass body, triple ceramic, single junction, refillable, combination pH electrode. Use: food processing.
FC911B Plastic body (PVDF), double junction, refillable with built-in amplifier, combination pH electrode. Use: very high humidity.
ORP ELECTRODES HI3131B Glass body, refillable, combination platinum ORP electrode. Use: titration.
HI3230B Plastic body (PEI), gel filled, combination platinum ORP electrode. Use: general purpose.
81

ACCESSORIES

HI4430B Plastic body (PEI), gel filled, combination gold ORP electrode. Use: general purpose.

Consult the Hanna Instruments General Catalog for more electrodes with screw- type or BNC connectors.

EXTENSION CABLE FOR SCREW-TYPE ELECTRODES (SCREW TO BNC ADAPTER)

HI7855/1 HI7855/3

Extension cable 1 m (3.3′) long Extension cable 3 m (9.9′) long

HI 7855 SERIES CABLE CONNECTORS CONNECTOR AND 3.0 mm (0.12″) CABLE WITH BNC

CONNECT TO SCREW TYPE ELECTRODES

CONNECT TO THE BNC SOCKET OF THE METER

OTHER ACCESSORIES

HI710005/8 Voltage adapter from 120 Vac / 12 Vdc 800 mA (USA plug)

HI710006/8 Voltage adapter from 230 Vac / 12 Vdc 800 mA (European plug)

HI76404W Electrode holder

HI8427

pH and ORP electrode simulator with 1 m (3.3′) coaxial cable ending in female BNC connectors

HI931001

pH and ORP electrode simulator with LCD and 1 m (3.3′) coaxial cable ending in female BNC connectors

HI7662-W Temperature probe with 1 m (3.3′) cable

HI92000

Windows® compatible software

HI920013 USB cable

MAN5222 05/19
82

Certification All Hanna Instruments conform to the CE European Directives.
Disposal of Electrical & Electronic Equipment. The product should not be treated as household waste. Instead hand it over to the appropriate collection point for the recycling of electrical and electronic equipment which will conserve natural resources. Disposal of waste batteries. This product contains batteries, do not dispose of them with other household waste. Hand them over to the appropriate collection point for recycling. Ensuring proper product and battery disposal prevents potential negative consequences for the environment and human health. For more information, contact your city, your local household waste disposal service, the place of purchase or go to www.hannainst.com.

Recommendations for Users

Before using this product, make sure that it is entirely suitable for your specific application and for the environment in which it is used. Any variation introduced by the user to the supplied equipment may degrade the instrument’s performance. For yours and the instrument’s safety, do not use or store the instrument in hazardous environments.

Warranty

HI5221 and HI5222 are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to instructions. Electrodes and probes are warranted for six months. This warranty is limited to repair or replacement free of charge. Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number and the nature of the problem. If the repair is not covered by the warranty, you will be noticed of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization (RGA) number from the Technical Service department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection.

Hanna Instruments reserves the right to modify the design, construction or appearance of it’s products without advance notice.

World Headquarters
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com
MAN5222

Printed in ROMANIA

References

• PC Compatible Software Versions | Hanna Instruments
• pH Meters, Photometers, Titrators, Controllers - Hanna Instruments

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