BLUE-RAY TurboCycler 2 User Guide

BLUE-RAY TurboCycler 2 User Guide

Scan the QR code on page 8 to download the Operation Manual.

Hardware operation

1. To open the lid, pull the handle upward and lift the lid backward to the upright position.

2. To close the lid, pull the lid downward and close the handle to the lock position.

3. Lid heater setting:
   PCR vessels| Compression mat| Lid heater temperature
   ---|---|---
   Regular PCR plate, tube-strips and tubes| Not necessary.The 1 mm compression mat can be used to help maintaining the sealing integrity in case the PCR plate has any imperfectionon the rim of the well.| 105°C
   Low profile PCR plate, tube-strips and tubes| The 5.2 mm compression mat must be used to ensure sufficient contact pressurefrom the heated lid| 120°C

a. Instruction for applying the compression mat:

b. For detailed instructions on the lid heater setting, please refer to section
3.4 of the TurboCycler 2 Operation Manual

4. In order to prevent the deformation of the PCR vessels, and to ensure even distribution of the pressure from the lid, when PCR tubes or tube-strips are used, please load extra empty tubes on the 4 corners of the sample block. Always make sure that all the sample tubes/ tube–strips are distributed evenly on the sample block.
5. Ventilation air flow path:
   Please make sure there is no obstacle in the air flow path. At least 10 cm of space is required at the front and the back of the instrument.
   

Software operation

1. Main Screen: Tap on the “TurboCycler” title to log in.
   

2. User folder management:
   a. On the main screen, tap on to enter the user folder management section.
   b. Tap on to create a new user folder. Input the folder name and password (optional). Tap the user icon to toggle to the icon you want to use for the new folder.

c. To modify an existing user folder, tap on the icon to select the folder. The selected folder’s icon will be highlighted.

3. To create a new protocol:
   a. On the main screen, tap on to create a new protocol.
   b. To select the Stage / Step, tap on the stage header or the step segment. The selected area will be highlighted.
   
   c. Tap on to duplicate the Stage / Step.
   d. Tap on to delete the Stage / Step.
   e. Tap on in the Stage header to modify the cycle number of the stage.
   
   f. Tap onin the Step to modify the step temperature, time or ramp rate.
   
   g. Tap on to set the optional Gradient, Temperature
   increment/decrement per cycle or the Time increment/decrement per cycle for the selected step.
   
   h. Tap on to save the protocol. Input the protocol name and tap on “OK”, then you’ll be brought to the user folder selection screen. Tap on the user folder icon to select the folder, and then tap on to save to the folder.Alternatively, you can tap on to save the protocol to the USB flash drive.

4. To edit an existing protocol:
   a. Please refer to the step c. in “Section 2. User folder management” to open the folder.
   b. Tap to select the protocol.
   
   Tap on to open the protocol.
   c. Follow the instructions of step b. to h. in “Section 3. To create a new protocol” to edit and save the protocol.

5. To run an existing protocol:
   a. Follow the instructions of step a. and b. in “Section 4. To edit an existing protocol” to open the protocol.
   b. Tap on to initiate the run.
   
   c. Specify the lid heater temperature.
   
   d. Specify the mode you want to use to run the protocol. Key in the sample volume if you select the “Sample Mode”. Tap on to start the run.

6. Tools :
   Icon| Function| Description
   ---|---|---
   **| Tm Calculator| Calculate the Tm for the input primer
   **| Incubation| Use the TurboCycler as an incubator
   **| Copy Number Convertor| Convert the DNA concentration into copy number
   **| Mastermix Preparation Wizard| Calculate the volume of thereagents needed to make the mastermix
   **| Help**| Display the quick operation guide

7. System:

   ---

Note:

1. The content of this document is subject to change without prior notice. Please visit Blue-Ray Biotech’s website at www.blue-raybio.com for update information.
2. If you have any questions about the operation of the TurboCycler, please contact sales@blue-raybio.com for assistance.
3. Sacn the QR code to access the product website and the operation manual.
   Product Website
   
   Operation Manual
   

References

• 睿贝医学科技-致力于生命科技
• Blue-Ray Biotech│PCR, Live-Cell Imaging, Spectrophotometer│ Life Science Equipment and Instruments
• Blue-Ray Biotech│PCR, Live-Cell Imaging, Spectrophotometer│ Life Science Equipment and Instruments

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