QSONICA Q125 Sonicator Ultrasonic Cell Disrupter User Manual

QSONICA Q125 Sonicator Ultrasonic Cell Disrupter

WARRANTY

Your Ultrasonic Processor is warranted and backed by the manufacturer for a period of two years from the date of shipment against defects in material and workmanship under normal use as described in this instruction manual. During the warranty period, the manufacturer will, at its option, as the exclusive remedy, either repair or replace without charge for material and labor, the part(s) which prove to be defective, provided the unit is returned to us properly packed with all transportation charges prepaid.

Ultrasonic probes are guaranteed against defects for a period of one year from date of shipment. A defective probe will be replaced once without charge, if failure occurs within the warranty period. Wear resulting from cavitation erosion is a normal consequence of ultrasonic processing, and is not covered by this warranty.

The manufacturer neither assumes nor authorizes any person to assume for it any other obligations or liability in connection with the sale of its products. The manufacturer hereby disclaims any warranty of either merchantability or fitness for a particular purpose. No person or company is authorized to change, modify, or amend the terms of this warranty in any manner or fashion whatsoever. Under no circumstances shall the manufacturer be liable to the purchaser or any other person for any incidental or consequential damages or loss of goodwill, production, or profit resulting from any malfunction or failure of its product.

This warranty does not apply to equipment that has been subject to unauthorized repair, misuse, abuse, negligence or accident. Equipment which, shows evidence of having been used in violation of operating instructions, or which has had the serial number altered or removed, will be ineligible for service under this warranty.

All probes are manufactured to exacting specifications and are tuned to vibrate at a specific frequency. Using an out-of-tune probe will cause damage to the equipment and may result in warranty nullification. The manufacturer assumes no responsibility for probes fabricated by another party or for consequential damages resulting from their usage.
The aforementioned provisions do not extend the original warranty period of any product that has either been repaired or replaced by the manufacturer.

WARNINGS
Please read the manual in its entirety. Necessary instruction and guidance are provided to help ensure the successful operation of this device. Observe the following:

• High voltage is present in the power supply, converter and high frequency cable. There are no user-serviceable parts inside any of these devices. Do NOT attempt to remove the power supply cover or converter case.
• Do NOT touch any open cable connections on the unit while the power is turned ON.
• Do NOT operate power supply with converter disconnected from high voltage cable. High voltage is present in the cable and may pose a shock hazard.
• Do NOT attempt to disconnect the converter high voltage cable while the unit is running.
• The power supply must be properly grounded with a 3-prong plug. Test electrical outlet for proper grounding prior to plugging in unit.
• Install the Sonicator in an area free from excessive dust, dirt, explosive or corrosive fumes and protected from extremes in temperature and humidity. Do not place the power supply within a Fume Hood.
• Hearing protection is highly recommended. It is recommended that a sound abating enclosure or ear protection be used when operating the Ultrasonic Processor
• NEVER immerse the converter in liquids of any kind, or let condensed moisture or liquid drip into the converter.
• NEVER grasp an activated horn or touch the tip of a vibrating probe. It can cause severe burns and tissue damage.
• NEVER allow a horn/probe to vibrate in air.
• NEVER hold or clamp the converter by the front driver or by the horn itself. This can cause permanent damage to the system. Support the converter by only clamping around the converter housing (upper portion).
• Do NOT allow the tip of a vibrating horn or probe to touch the counter top or any other hard surface. It could damage the probe, overload the power supply, or damage the surface.
• Avoid touching the bottom or sides of a sample vessel with an activated probe. It may crack or shatter the glass or melt the plastic. Use glassware that is free from cracks or chips.
• Turn OFF the power switch, unplug the power supply and disconnect the power cord from the back of the power supply before attempting to replace the fuses.
• Inspect high frequency cable for cracks in the protective outer jacket.
• Do not operate unit with a damaged cable. Doing so may cause serious injury.
• In case of AC power loss, wait 3 minutes minimum before reapplying power.
• Do not turn off Main power switch while running a probe. Stop sonication by lowering intensity setting knob to 0 or release thumb switch.

Symbols: Caution, Risk of electric shock, Hazardous voltage. Caution, Risk of danger. Refer to User Manual.

SPECIFICATIONS

Power Supply|
---|---
 | Part #Q120-100| Part #Q120-110| Part #Q120-220
Input Voltage| 90 VAC – 110 VAC

@ 50/60 Hz

| 108 VAC – 132 VAC

@ 50/60 Hz

| 198 VAC – 264 VAC

@ 50/60 Hz

Rated Current| ****

2.4 Amps max.

| ****

2.4 Amps max.

| ****

1.2 Amps max.

Fuse Rating| ****

3 Amps Slow Blow

| ****

3 Amps Slow Blow

| ****

1.6 Amps Slow Blow

Weight| ****

7 lbs. (3.2 Kg)

Dimensions| 8″W x13.75″L x 5.75″H

203mm x 349mm x 146mm

Output Voltage| 380 VRMS (max.)
Output Frequency| 20 KHz
Converter|
---|---
Weight| 0.75 lbs. (0.34Kg)
Dimensions| 6″ L x 1.25″ Dia.

(15cm x 3cm)

Materials| Aluminum Alloy
Standard 1/8” Probe|
---|---
Weight| 0.25 lbs. (0.11Kg)
Dimensions| 5.4″ L x 0.5″ Dia.

(13.8cm x 1.3cm)

Materials| Titanium Alloy
E nvironmental|
---|---
Pollution Degree| 2
Installation Category| II
Operating Limits

Shipping/Storage

| Temperature: 41 – 104ºF (5 – 40ºC) Relative Humidity 10 – 95% (Non Condensing)

Altitude: 6,651 ft. (2000 m)

Temperature: 35 -120 ºF (2 – 49 ºC) Relative Humidity 10 – 95% (Non Condensing)

Ambient Pressure Extremes: 40,000 ft. (12,192 m)

Restriction of Hazardous Substances (ROHS)| ****
Relative humidity| Maximum relative humidity 80% for temperatures up to 31ºC decreasing

linearly to 50% relative humidity to 40ºC

Other| For indoor use only

The Power Cord supplied with the ultrasonic processor must be used. If the 220V plug is not configured to match the wall receptacle, a properly grounded universal AC socket adapter must be added.

Important: Universal adapters do not convert voltage or frequency. Manufacturer is not responsible for damage caused by the use of an improper power cord or adapter. Transformers are not recommended.

WEEE Statement
This product contains electrical or electronic materials. The presence of these materials may, if not disposed of properly, have potential adverse effects on the environment and human health. Presence of this label on the product means it should not be disposed of as unsorted waste and must be collected separately. As a consumer, you are responsible for ensuring that this product is disposed of properly. To find out how to properly dispose of this product contact Customer Service.

PRINCIPLES OF OPERATION

The ultrasonic power supply transforms AC line power to a 20 KHz signal that drives a piezoelectric converter/transducer. This electrical signal is converted by the transducer to a mechanical vibration due to the characteristics of the internal piezoelectric crystals.

The vibration is amplified and transmitted down the length of the horn/probe where the tip longitudinally expands and contracts. The distance the tip travels is dependent on the amplitude selected by the user through the keypad. As you increase the amplitude setting the sonication intensity will increase within your sample.

In liquid, the rapid vibration of the tip causes cavitation, the formation and violent collapse of microscopic bubbles. The collapse of thousands of cavitation bubbles releases tremendous energy in the cavitation field. The erosion and shock effect of the collapse of the cavitation bubble is the primary mechanism of fluid processing.

The probe tip diameter dictates the amount of sample that can be effectively processed. Smaller tip diameters probes deliver high intensity sonication but the energy is focused within a small, concentrated area. Larger tip diameters can process larger volumes, but offer lower intensity.

The choices of a power supply and horns/probes are matched to the volume, viscosity and other parameters of the particular application. Horns are available for both direct and indirect sonication. The frequently asked questions (FAQ) section has more information on this subject.

Please consult with a product specialist for assistance with selecting a probe for your application.
Relationship of Amplitude and Wattage

Sonication power is measured in watts. Amplitude is a measurement of the excursion of the tip of the probe (probe is also known as a horn).
Some ultrasonic processors have a wattage display. During operation, the wattage displayed is the energy required to drive the radiating face of a probe, at that specific amplitude setting against a specific load, at that particular moment. For example, the unit experiences a higher load when processing viscous samples then when compared to aqueous samples.

The speed/cruise control on an automobile, can, to a certain extent, be compared to an ultrasonic processor. The speed/cruise control is designed to ensure that the vehicle maintains a constant rate of travel. As the terrain elevations change, so do the power requirements. The cruise control senses these requirements, and automatically adjusts the amount of power delivered by the engine in order to compensate for these ever changing conditions. The greater the terrain rate of incline and greater the resistance to the movement of the vehicle, the greater the amount of power that will be delivered by the engine to overcome that resistance and maintain a constant speed.

The ultrasonic processor was designed to deliver constant amplitude to your liquid sample, regardless of these changes in load (much like the vehicle’s cruise control described above). As a liquid is processed, the load on the probe will vary due to changes in the liquid sample (i.e. viscosity, concentration, temperature, etc.). As the resistance to the movement of the probe increases (increased load on the probe), additional power will be delivered by the power supply to ensure that the excursion at the probe tip remains constant. The displayed wattage readings will vary as the load changes; however, the amplitude will remain the same.

The resistance to the movement of the probe determines how much power will be delivered to maintain amplitude. For example, a ½” probe at 100% amplitude will require approximately 5 watts to operate in air. The amplitude of this probe is approximately 120um. Insert the probe in water and the wattage reading will increase to approximately 90 watts. The wattage required to operate the probe will increase as the load increases but the amplitude remains the same.

The amplitude control allows the ultrasonic vibrations at the probe tip to be set to any desired level. Although the degree of cavitation/ultrasonic energy required to process the sample can readily be determined by visual observation, the amount of power required cannot be predetermined. A sensing network continuously monitors the output requirements, and automatically adjusts the power to maintain the amplitude at the preselected level. The greater the resistance to the movement of the probe due to higher viscosity, deeper immersion of the probe into the sample, larger probe diameter or higher pressure, the greater the amount of power that will be delivered to the probe. Setting the amplitude control to its maximum will not cause the maximum power rating of the unit to be delivered to the sample. The maximum power (125 watts) that the Sonicator is capable of delivering will only be delivered when the resistance to the movement of the probe is high enough to draw maximum wattage.

It is the intensity of cavitation that measures the effectiveness of the sonication, not the total power applied to the system. Intensity is directly related to the amplitude of the radiating face of the tip or horn. It is amplitude that must be provided, maintained, and monitored. The unit provides controlled amplitude under varying load conditions in order to give reproducible results.

DESCRIPTIONS OF COMPONENTS / FUNCTIONS OF CONTROLS

Q125 FRONT PANEL

Q125 REAR PANEL

FUNCTIONS OF KEYS CONTROLS & CONNECTORS

FRONT PANEL

LCD display

| Displays prompts and the following control parameters:

·        Amplitude selected

·        Amount of output power delivered to the probe in watts, and as a percentage of 125 watts.

·        Accumulated amount of energy in Joules delivered to the probe.

CLEAR

key

| Clears the preceding entry.
ENTER REVIEW

key

| Used to enter the amplitude selected, and view amplitude, power, and energy
START/STOP

key

| Starts or stops the ultrasonics. In the STOP mode the red indicator goes off.
I

key

| Switches the main power on.
0

key

| Switches the main power off.
AMPL| Controls the amplitude of vibration at the probe tip.

▲    ▼ key

| Used with the AMPL key when the unit is on stand-by to set the amplitude of vibration at the probe tip. Also used to increase or decrease the amplitude in small increments while the unit is running. To accomplish this task, depress the ENTER/REVIEW key twice to display AMPLITUDE CONTROL, then depress the ▲ or ▼ key as required.
REAR PANEL
9 pin D-sub connector (IO Port)| Connects to external actuation device, and enable power and frequency monitoring.
Footswitch Connector| Connects to the footswitch cable.
Converter Cable Connector (Output)| Connects to the converter.
Power Supply Connector| Connects to the electrical line cord and encases the fuse(s).
9-PIN D-SUB CONNECTOR
Pin No.| Description|

NOTE: To vary the intensity remotely using a variable DC power supply (0-5V) instead of a 10 K potentiometer, connect positive to pin 8 and negative to pin 6.

1| Not connected
2| Not connected
3| Not connected
4| Enables connection to a frequency counter.
5| Enables connection to external power monitor (5 mv = 1 watt)
6| Ground
7| Energizes the ultrasonics when connected to ground.
8 and 9| Enables the intensity to be remotely adjusted using an external 10k potentiometer.

CONVERTER CLAMPING

Improper clamping can damage the system and void the warranty. Using a sound enclosure (part #432A) or stand (part #460) will ensure a proper fit.

• Proper Clamping
• Improper Clamping

PREPARATION FOR USE

INSPECTION
Prior to installing the Ultrasonic Processor, perform a visual inspection to detect any evidence of damage, which might have occurred during shipment. Before disposing of any packaging material, check it carefully for small items.

The Ultrasonic Processor was carefully packed and thoroughly inspected before leaving our factory. The carrier, upon acceptance of the shipment, assumed responsibility for its safe delivery. Claims for loss or damage sustained in transit must be submitted to the carrier.
If damage has occurred, contact your carrier within 48 hours of the delivery date. DO NOT OPERATE DAMAGED EQUIPMENT. Retain all packing materials for future shipment.

ELECTRICAL REQUIREMENTS

The Ultrasonic Processor requires a fused, single phase 3-terminal grounding type electrical outlet capable of supplying 50/60 Hz at 100 volts, 115 volts, 220 volts, or 240 volts, depending on the voltage option selected. For power requirements, check the label on the back of the unit. Should it become necessary to convert the unit for different voltage operation, proceed as follows:

1. Ensure that the power cord is not connected to the electrical outlet.
2. Open the fuse holder cover using a small screwdriver.
3. Pull out the red fuse holder from its housing.
4. To convert from 100/115V to 220/240V replace the two 3 Amp slow blow fuses, with 1.6 Amp fuses.
5. To convert from 220/240V to 100/115V reverse the procedure above.
6. Rotate the fuse holder 180º from its original position, and reinsert it into its housing. For 100/115V operation the voltage displayed should be 115. For 220/240V operation the voltage display should be 220.
7. Change the electrical power cord as required.
8. Clearly note on the label or back of the unit that the voltage has changed.

WARNING: For your personal safety, do not, under any circumstances, defeat the grounding feature of the power cord by removing the grounding prong.

INSTALLING THE ULTRASONIC PROCESSOR
The Ultrasonic Processor should be installed in an area that is free from excessive dust, dirt, explosive and corrosive fumes, and extremes of temperature and humidity. If processing flammable liquids use an approved fume hood and do not place the power supply in the fume hood.

When positioning the unit, be sure to leave adequate space behind the unit so that all connections can be easily disconnected.

OPERATING INSTRUCTIONS

CAUTION

• Do not operate the power supply unless it is connected to the converter.
• Never allow liquid to spill into the converter.
• Do not allow a microtip to vibrate in air for more than 10 seconds.
• Do not allow the vibrating microtip to contact anything but the sample.
• Never place a washer between the probe and the converter.
• Never apply grease to the mating surfaces or threads of the converter or microtip.
• Should it become necessary to remove a probe, use the wrenches supplied. Never attempt to remove the probe by twisting the converter housing, as this may damage the electrical connections within the housing.

Note: The temperature of the converter front area (where the probe/horn attaches) should never exceed 60oC (140oF). If this temperature is reached, shut the system off and allow to cool. Do not allow the system to operate for more than 1 minute without a rest or pulse to prevent overheating.

SETUP

1. Plug the electrical line cord into the electrical outlet. If the unit is already on, depress the OFF key.
2. If the optional foot switch is used, insert the plug into the jack located on the rear panel.
3. Probes must be properly tightened. If the convertor and probe assembly is not already assembled; screw the probe onto the convertor and using the wrenches provided and tighten securely. Check the tightness of an already assembled probe by using the wrench set. Please refer to images in the Maintenance section of this manual for tightening instructions. A loose probe may cause damage to the power supply circuitry or parts of the converter and probe. Always use the wrenches supplied with the unit.
4. Sample processing should be used in pulse mode to prevent overheating – which could potentially crack the tip and damage the converter.
5. Probe tips wear after normal use. Using a severely worn probe tip can damage internal power supply components.
6. If using a laboratory stand, mount the convertor/probe assembly using a clamp. Be sure to secure the clamp to the upper section of the convertor housing only (see page 10).
7. Connect the converter cable to the power supply.
8. If the application requires long processing times we recommended chilling the sample and pulsing sonication.

OPERATION

Press the ON key. The screen will display the power rating of the Ultrasonic Processor and the following control parameters.

AMPLITUDE: Desired amplitude must be set in order for the Ultrasonic Processor to be operational. The other control parameters – Time and Pulse, do not have to be set for continuous operation. AMPL displays the amplitude selected (e.g. 40%). To set the amplitude at 40% when the ultrasonics is off, press the AMPL key and the numeric keys for a 40% reading on the screen, and then press the ENTER/REVIEW key.

Note: The minimum amplitude setting is 20%. The screen will display:

1. Immerse the probe approximately halfway into the sample. If the probe is immersed to an insufficient depth, air will be injected into the sample, causing the sample to foam. Also ensure that the probe tip is not touching the wall of the sample vessel.
2. The Ultrasonic Processor is now ready for continuous operation. To energize the ultrasonics, press the START key or the footswitch. To de-energize the ultrasonics, press the STOP key or release the footswitch. If the Time or Pulse functions must be used, refer to the appropriate paragraphs below.

Note: The START key and footswitch are mutually exclusive. If the process is initiated by the START key, the footswitch becomes inoperative. If the process is initiated by the footswitch, the STOP key becomes inoperative.

• To increase or decrease the amplitude in small increments when the ultrasonics is on, depress the AMPL to display Amplitude Setting on the screen, then depress the ▲ or ▼ key, as required. Since the amplitude required is application dependent and subject to the volume and composition of the sample, it is recommended that the amplitude be selected through experimentation, by increasing or decreasing the level of intensity as needed to properly process the sample to achieve desired results.

TIMER: In the pulsed mode the processing time will be different from the elapsed time because the processing time function monitors and controls only the ON portion of the duty cycle. For example, for 1 hour processing time, the elapsed time will be 2 hours if the ON and OFF cycle are set for 1 second.

1. To set the processing time, press the TIMER key.
   The screen will display:

2. Using the numeric keys, set the processing time as required, for example:

3. Press the ENTER/REVIEW key. The screen will display:

PULSER: Ultrasonics generates heat. Pulsing ultrasonics on and off helps to prevent heat build-up in temperature sensitive samples. In addition, pulsing may enhance processing by allowing the material to settle back under the probe after each burst. The ON and OFF pulse duration can be set independently from 01 second to 59 seconds. During the OFF portion of the cycle, the red indicator on the PULSE key will illuminate. If the OFF portion of the cycle exceeds three seconds, a cautionary message – Sonics in OFF Cycle – will warn the operator against touching the ultrasonic probe.

1. To set the pulser, press PULSE key.
   The screen will display:

2. Using the numeric keys, set the ON portion of the cycle, and press the ENTER/REVIEW key.
   The screen will display:

3. Using the numeric keys set the OFF portion of the cycle.
   The screen will display:

REVIEW: The REVIEW function provides a “window” on the process by displaying various operating parameters without process interruption. Pressing the ENTER/REVIEW key repeatedly during processing will consecutively display the following information.

• a) Selected amplitude:

  • e. g. Amplitude 40%

• b) Selected processing time and elapsed processing time:

  • e. g. Set 0:10:00 Time 0:05:00

• c) Selected pulsing cycle and actual pulsing cycle:

  • e. g. Pulse 05 05 / 05 00

• d) Amount of power in watts, and accumulated amount of energy in JOULES delivered to the probe (Note: The amount of energy displayed will be only for one cycle.
  Initiating a new cycle will reset the display to zero.)

  • e. g. 20 watts 0000000 Joules

• e) Elapsed time since processing was initiated:

  • e. g. Elapsed time 0:05:00

MAINTENANCE

It is recommended to periodically inspect the unit, both visually and physically, to ensure optimum and safe performance. This inspection should be scheduled as a routine maintenance procedure, done with the unit power OFF and with the unit unplugged from the AC power source.
Long exposure to acids or caustics results in corrosion of metal parts or components. Check the power supply, converter, and cables periodically for any signs of rust or discoloration. If discoloration is found, move the unit away from the source of the contaminant.
Examine the condition of the high voltage cable that attaches the converter to the power supply. Inspect the wire insulation for damage, such as wear, burning from hot plate contact or breakage from extended use or rough handling. In general use, the cable assembly should not be used to carry the converter or pull it toward the user. Make certain the cable always has slack and is never tensioned. If necessary, move the power supply or converter assembly closer to one another to accomplish this. WARNING: Do not use a cable with broken end connections, exposed wires or frayed insulation. High voltage is present in the cable and will pose a shock hazard. Do not touch the converter assembly until the power switch is off and the unit is unplugged.

PROBE MAINTENANCE

Ultrasonic processors create high intensity vibration which puts stress on the converter and horn assembly. The sides and end of the probe must never be allowed to come in contact with anything but the solution. When using a probe, the stress resulting at the point of contact with the vessel could cause the probe to fracture.

Proper care of the probe is essential for dependable operation. The intense cavitation will, after usage for period of time, cause the tip to erode, and the power output to decrease. The smoother and flatter the tip, the more power will be transmitted into the sample. The cavitation may also cause the probe to loosen over time or the threaded connection to accumulate debris. Note: A loose probe will usually generate a loud piercing or squealing sound.

For that reason, it is recommended that a preventative maintenance schedule be adopted to examine the unit at regular intervals. The schedule should depend on frequency of use. Weekly maintenance schedules are recommended for units used frequently or monthly for those used infrequently. The tip must be examined for excessive wear and to ensure that the threaded connection is clean and attached properly to the convertor. Use a cotton swab and alcohol (i.e. ethanol, isopropyl, etc.) to clean the threaded mating surfaces.

When excessive wear (corrosion/pitting of the probe tip) is detected the probe should be replaced with a new one.
WARNING: Probes must be properly tightened with the appropriate Wrench Set.

ATTACHING & DETACHING MICROTIPS

1. Disconnect probe from convertor. Use the wrench set provided with the system.
2. Clean threaded stud. Use alcohol and a cotton swab to remove any debris on the threading of the connecting stud. Allow the alcohol to dry completely.
3. Clean threading in converter. Use alcohol and a cotton swab to remove any debris on the threading. Do not allow liquid to drip into converter. Allow the alcohol to dry completely.
4. Re-attach probe to converter. Screw the probe back onto the converter and tighten with the wrench set provided.

SYSTEM CLEANING INSTRUCTIONS

The power supply and converter may be cleaned using an acid-free cleaning solution (i.e. glass cleaner).
Probes should be cleaned using isopropyl alcohol. Probes are made from titanium and can be autoclaved (the converter is an electrical part and cannot be sterilized in this manner). Before each procedure place the probe tip in water or alcohol and turn the power on for a few seconds to remove residue. The tip also can be sterilized using alcohol with the power on.

TROUBLESHOOTING

The most probable causes for malfunction are listed below and should be investigated.

• A connector or cable is damaged.
• The unit was plugged into an electrical outlet that provides a different voltage from that required. See Electrical Requirements.
• The probe is not tightened properly with the wrenches provided.
• The convertor and/or probe has been dropped.
• A probe being operated is damaged or worn past its useful life.
• A fuse(s) has failed. If a fuse(s) has failed, proceed as follows:

1. Turn the unit off by depressing the OFF key, and disconnect the line cord from the electrical outlet.
2. Open the fuse holder cover using a small screwdriver, and pull out the red fuse holder from its housing.
3. Replace the fuse(s).
4. Reconnect the line cord to the electrical outlet, press the ON key and set the amplitude to 100. With the probe in air (out of the sample), the wattmeter should read below 10 watts. If the reading exceeds 10 watts, press the OFF key, and disconnect the probe from the converter.
5. Press the ON key. If the wattmeter reads below 10 watts, the probe has failed or is out of tune due to excessive erosion, and should be replaced, if the wattmeter reads above 20 watts, either the converter or power supply has failed and the complete Ultrasonic Processor should be returned for repair.

OVERLOAD CONDITION

If the Ultrasonic Processor stops working, and an OVERLOAD indication is displayed on the screen, check for possible causes as outlined in the above paragraph, then press the OFF key to switch the unit off, and the ON key to switch the unit back on.

If the problem persists after inspecting all of these, please contact Customer Service for additional assistance or to replace a worn microtip or damaged part.

RETURN OF EQUIPMENT

• It is suggested that an Ultrasonic Processor in need of repair be sent back to the factory.
• In order to receive prompt service, contact your Customer Service Representative before returning any instrument.
• You must obtain a Return Authorization Number (RMA) prior to returning the instrument.
• Care should be exercised to provide adequate packing to insure against possible damage in shipment. The Ultrasonic Processor should be sent to the address below with all transportation charges prepaid and return of shipment indicated.
  • RMA # ——-
  • Qsonica
  • 53 Church Hill Rd. Newtown, CT 06470

Important
The user must certify that the ultrasonic processor and/or the accessories returned for repair are free of any biohazardous or radioactive material and are safe for handling. Please complete the “Safety Certification” form on the next page and send it in with your equipment.

Do not return any equipment unless such a certification can be made.

SAFETY CERTFICATION FORM

Items being returned:
Please check only one item below:

• The equipment was never used or exposed to any radiological, biological or chemical agents and is safe to handle, use or dispose of.
• The equipment was used but not in conjunction with or exposed to any radiological, geological or chemical agents and is safe to handle, use, or dispose of.
• The equipment was used in conjunction with or exposed to radiological, biological, or chemical agents and has been decontaminated, rendering it safer for handling, use, or disposal.

Authorization
By accepting authorization to return the equipment listed above, the undersigned assumes all responsibility and liability for radiological, biological and chemical decontamination. Delivery of the equipment can be refused if necessary documentation is not provided or where it is determined that the equipment has not been properly decontaminated. If it is determined that the equipment was not properly decontaminated, the Authorized Repair Facility reserves the right to bill the customer for any and all costs associated with the decontamination and/or appropriate disposal of the equipment. In the event the equipment has been exposed to radiological contamination, the signature of the Radioactive Safety Officer is required.

• Print name: _____
• RA # _____
• Signature: _____
• Date: ____

FREQUENTLY ASKED QUESTIONS

Probe size vs. Sample volume
Selecting the proper size probe is a critical factor when sonicating a sample. The sample volume to be processed must correlate with the tip diameter. Each probe has a recommended sample volume range. This range may overlap with other probes. For example, the 1/8” probe is recommended for approximately 0.5-15ml. Depending on the application and type of sample, you may be able to process a volume outside of our recommended range.

Small volumes require a small tip diameter. Small probes (¼” or less) are recommended for processing samples inside small, thin vessels such as microcentrifuge tubes. These small probe sizes are high intensity and made for short processing times. Using a small probe tip for long time periods will generate a considerable amount of heat. Pulse mode should be used to reduce heat buildup.

While there is no absolute sample volume range for any probe/horn, below is a general guideline to follow. Using a sample volume outside each tip diameter’s range is normally not recommended.

Vessel shape and size
A narrow vessel is preferable to a wide vessel. The ultrasonic energy is generated from the tip and is directed downward. As a sample is processed the liquid is pushed down and away in all directions. If the vessel is too wide, it will not mix effectively and some sample will remain untreated at the periphery. The probe should never touch the sides or bottom of the vessel.

How to prevent foaming (small sample issue)
Foaming is a problem that often occurs with samples volumes below 1ml. The cause of foaming is generally 3 issues: amplitude is too high for a small volume, tip is too large for the volume, or the tip is not inserted to a proper depth.

Tip depth
The depth of the probe within the liquid is an important issue. If the probe is too close to the surface of the liquid, it can create foam. If the probe is too deep, it may sonicate against the bottom of the vessel and not effectively processing the sample. The sample must flow freely below the tip in order to be mixed effectively. Without effective mixing you cannot ensure the entire sample volume will pass below the tip and become processed.
The probe should be submerged approximately halfway into the liquid but there are exceptions. Before processing actual samples, it is recommended to test the probe in a vessel filled with water to observe the ultrasonic energy and the flow pattern of the liquid. During this test you can adjust the probe’s depth until you see adequate mixing and movement of the water.

Power vs. intensity
Power is the measure of the electrical energy that is being delivered to the converter. It is measured in watts and displayed on the sonicator’s screen. At the convertor, the electrical energy is transformed into mechanical energy. It does this by exciting the piezoelectric crystals causing them to move in the longitudinal direction within the convertor. This change from electrical into mechanical energy causes a motion that travels through the horn/probe causing the tip to move up and down.

The distance of one movement up and down is called its amplitude. The amplitude is adjustable. Each probe has a maximum amplitude value. For example, with a 1/8” diameter probe at setting 100%, the probe will achieve an amplitude of approximately 180μm. At setting 50% the amplitude is approximately 90μm. Note: this is approximate and not perfectly linear. We measure the amplitude of each probe at 100% and these values are published in the brochure.

Amplitude and intensity have a direct relationship. If you operate at a low amplitude setting, you will deliver low intensity sonication. If you operate at a high amplitude setting, you will have high intensity sonication. In order to be able to reproduce results, the amplitude setting, temperature, viscosity and volume of the sample are all parameters that need to remain consistent. The amplitude, not the power, is most critical when trying to reproduce sonication results.

Power has a variable relationship with amplitude/intensity. For example, sonicating water requires less wattage when compared to a viscous sample (such as oil). While sonicating both samples at the same amplitude setting the power/wattage will differ because the viscous sample will require more watts in order to drive the horn. The viscous sample puts a heavier load on the probe so they system must work harder to vibrate up and down at the same amplitude setting. The oil may draw double the watts when operated at the same amplitude as the water sample.

Small fluctuation in the wattage during sonication is normal. Major swings in wattage (+/- 20 watts) may indicate a problem with the sample, setup or the sonicator itself.
Viscosity Limitations

Viscous solutions and highly concentrated liquids can be difficult to sonicate. If the liquid is so thick that it cannot be easily poured out of a vessel it is likely too viscous to be sonicated.

Keeping Samples Cool
Ultrasonic processing causes the liquid temperature to elevate especially with small volumes. Pulsed sonication is always recommended. The addition of an ice bath or recirculating chiller to cool the sample vessel is strongly suggested.

See website for more details and additional information: www.sonicator.com

References

• Sonicator Ultrasonic Homogenizers & Emulsifiers | Qsonica

Read User Manual Online (PDF format)

Read User Manual Online (PDF format)  >>

Download This Manual (PDF format)

Download this manual  >>