ACCURIS Quadcount Automated cell counter Instruction Manual
- June 3, 2024
- ACCURIS
Table of Contents
Quadcount Automated cell counter
Automatic Cell Counter
Instruction manual
Accuris Instruments
A division of Benchmark Scientific
PO Box 709, Edison, NJ 08818
Tel: 908-769-5555
E-mail: info@accuris-usa.com
Website www.accuris-usa.com
Copyright © 2020, Benchmark Scientific.
All rights reserved.
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Package contents
QuadCount™ Automatic cell counter package includes the following items.
Item Quantity
QuadCount™ main device 1
USB Memory Stick 1
Quick manual (PDF on Memory Stick) 1
Instruction manual (PDF on Memory Stick) 1
Main Power Cable 1
QuadCount™ Slides (Optional) 50 ea. per box
Keypad (Optional) 1
Barcode scanner (Optional) 1
Thermal printer (Optional) 1
When receiving the package,
• Check that all items listed above are included in your package.
• Examine the device carefully for any damage during shipping.
• Contact your local distributor or info@accuris-usa.com if any items are
missing or damaged.
• Any loss or damage claims must be filed with the carrier.
4
Safety instruction
READ ALL INSTRUCTION BEFORE USE
Caution
• Check the power supply input voltage and make sure it matches the wall
outlet voltage.
• Check that the power cable is connected to a grounded, 3-pin wall outlet.
• Check that the power cable is properly grounded to avoid potential
electrical shock.
• Check that the main power switch is off when plugging in the power cable to
the wall outlet or
when unplugging the power cable.
• Power on using the main switch on the rear panel, wait about 2-3 minutesfor
the device to reboot.
• Do not insert any metallic objects into the device through backside air vent
to avoid electrical shock
causing personal injury or device damage.
• Place the device in an area where there is 10 cm clearance from other
objects to allow for proper
air-cooling.
• Do not disassemble the device. If service is needed, contact Accuris
Instruments or an authorized
distributor.
• Use authorized accessories only.
• Operator should have the general knowledge of laboratory techniques and cell
counting
procedures as well as safe handling of biological samples.
• Operate the device carefully as described in this manual.
Warning
• Battery
There is a Lithium battery is inside the device. Replacing it with an
incorrect type can cause risk of
explosion. This battery should not be replaced by the user; contact Accuris an
authorized service
center if required.
• Sample handling
Samples may contain infectious biohazardous substances. Operator should wear
gloves while
handling all samples.
• Waste
Dispose used QuadCount™ Slides as biohazardous waste and do not reuse them.
5
Product specifications
QuadCountTM
Voltage AC 100~240 V, 50~60 Hz
Current Max. 1.0 A, 50 W
Objective lens 4 x
Light source 4 W Green LED
Camera
5Mega pixels high resolution
monochrome CMOS image
sensor
Weight 5 Kg
Size (W × L × H) 163 × 293 × 216 mm
Measuring
concentration range
1 x 104 ~ 1 x 107
cells/mL
Detectable cell
diameter 5 ~ 60µm
Measuring speed*
Quick mode: ≈ 20s per test
Normal mode: ≈ 30s per test
Precise mode: ≈ 100s per test
Counting area
Quick mode: ≈ 0.15 µL
Normal mode: ≈ 0.9 µL
Precise mode: ≈ 3.6 µL
QuadSlides™
(Cat. No.
E7500-S1
(Order
separately)
Quantity 50 slides per box (for 200 tests)
Sample loading
volume 20 µL
Accessories
Power cable 1.5 m
USB memory stick Supports USB 2.0
(Optional)
Keypad, Barcode
scanner, thermal
printer
USB type
*Cell Counting time can vary depending on cell type and concentration.
6
Device overview
Front view
• Slide holder door – Slide holder is ejected from / inserted into the device.
• Touch LCD display – Preview, automatic cell counting processes and the
results are displayed.
• 3 control buttons
Slide holder door
Touch screen LCD display
Button 3(Setting screen)
Button 1(Insert/Eject slide holder)
Button 2(Home screen)
7
Rear view
• 3 USB ports – Keypad, Barcode scanner, Thermal printer (optional), or USB
memory are
connected to these ports.
• Ethernet port – LAN cable is connected to this port for PC interface.
• Power switch – Main device power ON/OFF control.
• Power cable socket – Power cable is connected to this socket.
USB Port
– Keypad
– Barcode Scanner
– Thermal Printer
– USB Memory
Ethernet Port
– PC Interface
Power Switch
Power Cable Socket
8
Table of contents
Package contents 3
Safety instruction 4
Product specifications 5
Device overview 6
Introduction
QuadCount™– Automatic cell counter 10
QuadSlides™ (50 slides for 200 tests per a box, Cat. No. E5750-S1)
Getting started
Pre-requirements 12
Basic installation 13
Power up and initial display 14
General operation
Sample preparation 15
Basic operation 16
Preview prior to counting 18
Stopping while counting
Set counting option 21
A. Changing user group 22
B. Setting Count mode 23
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23
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C. Creating a Preset 24
D. Editing a Preset 27
E. Selecting Channels
F. Entering a Channel ID 30
The result screen
A. Analyzing by Histogram 36
B. View results images
C. Printout Cell Count Result using Thermal Printer 40
D. Exporting the Report to a USB memory stick
E. Exporting Data (all history) to a USB memory stick 43
F. Showing channel ID names
Setting screen
A. Checking Firmware information and Updating Firmware 48
B. Bead Quality Control 50
C. Setting Date and Time
Maintenance and cleaning
Appendix
A. Trouble shooting 54
B. Examples of errors and inaccurate results
C. The contents of result data exported as a .csv file
D. Example and explanation of PDF report 58
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10
Introduction
QuadCount™– Automatic Cell Counter
The QuadCount™ is a fully automated cell counting system based on a
brightfield microscopy
technique for mammalian cell counting. The QuadCount™ utilizes a high-powered
LED light source,
CMOS image detection (5 Mega pixels), precise X-Y-Z stages and on-slide image
processing
technologies for fast and accurate cell analysis.
Cell counting using the QuadCount™ requires 3 main steps, (1) cell staining,
(2) loading
sample slide, and (3) counting. Cells are mixed with trypan blue dye to
distinguish between live and
dead cells. The stained sample is pipetted into the disposable plastic slide
(4 tests per slide) and the
slide is loaded into the QuadCount Instrument. After loading the slide, the
optic system
automatically focuses on the slide and the instrument acquires and analyzes
the images
automatically. The precise X-Y-Z stages move through the preset routes to take
multiple images for
each channel. A highly sensitive CMOS sensor acquires bright-field microscopy
images and sends
them to the integrated system for image processing and analysis. The whole
counting process takes
2 minutes (in Normal mode) and the counting results are displayed on the LCD
touch screen panel
on front of the instrument.
11
QuadSlides™ Slides (50 slides for 200 tests per a box, Cat. No. E7500-S1)
The QuadSlide™ is a disposable plastic hemocytometer that includes 4 sample
channels engraved
with Neubauer Improved pattern. Each channel has an enclosed structure of
100um depth and a
hydrophilic surface. The precise capacity and diffusible surface ensures that
cells are
evenly distributed and this ensures accurate analysis. QuadSlides™ can be used
for mammalian
cell counting with the QuadCount Instrument, but can also be used for manual
counting methods.
The measuring range of cell concentrationsis 1 x 104 ~ 1 x 107 per mL when
used with the QuadCount
Instrument.
Cell Counting: prepare a cellsuspension for counting and mix the cell
suspension with trypan
blue at a one to one ratio. Each channel of a QuadSlide™ is filled with 20 μL
of mixture and is then
loaded into the QuadCount™ Instrument. After the analysis is complete, the
results will be displayed.
Keep QuadSlide™ boxes upright and at room temperature. Each individual slide
should be
used immediately after opening the individual sealed package. Follow the exact
procedure detailed
in the Instructions for Use section.
12
Getting started
Pre-requirements
For normal and stable operation of the device, the following environmental
conditions should be
met.
• Room temperature between 20 ~ 35 °C (68 to 95 °F)
It is not recommended to operate the device at low temperature condition
(below 10 °C)
In cold conditions, warm up the device for a minimum of 10 minutes before use.
• Relative humidity between 0 ~ 95 %.
• Install in a location free of corrosive gases or other corrosive substances.
• Install in an area free of dust or other airborne particles.
• Avoid direct sunlight, vibration, and close proximity to magnetic or
electromagnetic fields.
• Do not place any heavy objects on the top of the device.
13
Basic installation
1. Unbox the QuadCount™ and place the
device on a flat, level and dry surface.
2. Plug accompanying power cable into the
power cable socket.
3. Connect any optional peripherals (keypad,
barcode scanner, or thermal printer) to the
USB port if desired.
4. Plug the power cable into an appropriate
rated wall outlet and press the power switch
to ON.
Check that the main power switch is in I (ON)
position.
14
Power up and initial display
1. Once the main power is switched on, the
boot image is displayed on the LCD touch
screen. When booting is completed, the
initializing process starts and internal
motorized stages start moving.
2. Initializing progress is displayed while
processing.
3. When initializing is finished, the slide holder
is ejected, and Home screen is displayed on the
LCD touch screen.
4. After loading a slide with sample, the device
is ready to count.
15
General Operation
Sample Preparation
Required materials: Cell suspension, 0.4% trypan blue, micro tube 1.5ml,
pipette, tips, and
QuadSlides™. Preparation should be done in a clean area to avoid dust
contamination (dust on the
slides or in the samples will greatly reduce counting accuracy).
STEP 1. Prepare necessary items.
STEP 2. Place 20 μL of trypan blue in the micro tube and add an equal volume
of the cell
suspension.
NOTE: Before sampling the cell suspension, gently resuspend the cells at least
6 times
(pay attention to avoid bubbles and check if there are any cell clumps or
agglomerates)
The sampling should be in middle of the cell suspension, not on the surface or
the bottom.
STEP 3. Mix the sample in the micro tube by pipetting the vial 3~5 times
gently.
NOTE: Be careful not to create bubbles.
STEP 4. Load 20 μL of the stained cell sample into each channel of a
QuadSlide™.
NOTE: The samples should be from the middle of the cell suspension, not from
the surface or the
bottom, and ensure that no bubbles enter the slide channesl.
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Basic Operation
STEP 1. Insert a QuadSlide™ loaded with the samples into the slide holder.
NOTE: Make sure the arrow on the slide points toward the instrument.
STEP 2. Press Start button to start counting procedures. The slide holder will
retract
automatically, and auto-focusing is performed prior to counting each sample.
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STEP 3. The counting progress is indicated as shown in following image. For
completion of each
sample, the count results (unit: x104
/mL) are displayed.
STEP 4. Once counting is complete, the slide holder is ejected automatically.
Remove the QuadSlide™
from the slide holder.
1
1
Home
260 40 15
340 140 41
500 420 84
200 100 50
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Preview prior to counting
On the screen where you can see the cells, tap the screen twice to make the
icons disappear.
To get icons back again, tap the screen twice.
STEP 1. Load a slide and press Review button.
STEP 2. Select a channel to preview.
STEP 3. Positioning and Autofocusing happens automatically
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STEP 4. See the cell image of the selected channel.
STEP 5. Press Mark , and the detection mark is displayed. Live/Dead definition
can be modified
at this stage.
STEP 6. Counting
1
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Stopping While Counting
STEP 1. To stop the instrument during counting, Press the STOP button.
STEP 2. A confirmation message box is displayed as shown the following image.
Press the Continue button to confirm stopping.
STEP 3. Once stop counting is confirmed, all remaining processes are stopped,
and the slide holder
is ejected automatically.
1
1
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Set counting options
The following operations can be performed from the Home screen.
Setting options for counting
User : 1/2/3
Auto-saved data and presets can be managed per user.
Count mode : Quick/Normal/Precise
Total counting area (number of snapshots) is different for each count mode.
Quick mode: ≈ 0.15 µL (1 Frame)
Normal mode: ≈ 0.9 µL (6 Frames)
Precise mode: ≈ 3.6 µL (24 Frames)
Presets
User-changeable parameters for cell recognition
There are 3 kinds of fixed presets
Channel
Decide channels to be measured
White box: enabled channel
Gray box: disabled channel
Press a channel to toggle between enabling and disabling.
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A. Changing User Group
The QuadCount™ provides personalized history of results to user groups (1,2
and 3).
The user group is useful to manage user presets and numerous results autosaved
after counting. The
auto-saved results (review screen) are accessible only to the user group that
was active at the time
the results were captured.
Note: Review and User preset list depends on User group. Therefore, before
selecting user preset or
pressing review, check the user group.
Step 1. Press the User button.
Step 2. Select the User 1/2/3.
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B. Setting the Count mode
The QuadCount™ provides three counting modes (Quick/Normal/Precise mode)
according to the
counting area. The QuadCount™ is designed to capture multiple frames per
channel using an XYZ
stage. Each single image frame covers a volume of 0.15 µL. The more pictures
taken, the higher the
accuracy of results.
Select the count mode depending on requirements, refer to the following table.
Count mode
the number of
frames captured
per a channel
Analyzed
volume
Counting
time
per
chamber
Application requirement
Quick mode 1 0.15µL ≤ 20s
When you want to get a result quickly and
make a rough estimate of cell numbers.
Normal mode
(default) 6 0.9µL ≤ 30s
When you want to get results with
reasonable accuracy and speed (such as
general subculture procedure)
Precise mode 24 3.6µL ≤ 100s
When you require precise results or count
cells from a low concentration sample.
NOTE: if the cell concentration is less than 5X104
cells/ml, Precise mode is recommended.
STEP 1. Press the Count Mode button.
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STEP 2. Select the Count mode.
NOTE: The setting is applied to all enabled channels.
C. Creating a Preset
Users can manage User Preset items. (5 User presets are available per User
group)
The 3 fixed presets cannot be removed or edited.
STEP 1. To create your own preset, press Preset button.
STEP 2. Press Plus button.
PT
25
STEP 3. Select one of 3 fixed presets (Universal, Small, Angular),
and press the blank text box beside Index.
STEP 4. Type the names of Index and Preset ID.
primary T PT primary T
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STEP 5. Adjust the 3 parameters according to requirements.
(Gating size, Aggregation level, Live/Dead definition).
STEP 6. Ready to count with a customized preset.
PT
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D. Editing a Preset
STEP 1. To edit your own preset, press Preset button.
STEP 2. Select the preset button which you created.
STEP 3. Adjust the parameters of your preset.
PT
PT
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STEP 4. Press the Save button to keep the changed parameters.
STEP 5. To delete your own preset, press Delete button.
PT primaryT
PT
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E. Selecting Channels
Four channels in the QuadSlide™ can be individually enabled or disabled.
STEP 1. Press the Channel numbersto be disabled/enabled. (Disabled: Gray box,
Enabled: White box)
STEP 2. Press the Start button to count immediately.
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F. Entering Channel ID
Naming/Identifying a channel can be accomplished with the Channel ID option.
Select “Channel
ID” as shown below and enter the desired channel name. (The name might often
be the specific
cell type.)
The ID can consist of a maximum of 20 alphanumeric characters and some special
characters.
STEP 1. Press Channel ID button.
STEP 2. Choose a desired channel (1 through 4).
STEP 3. Type the desired names for each Channel.
Channel ID
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STEP 4. Press Back button.
STEP 5. Ready to count.
JurkatT
JurkatT
NIH
Hela
U937
Channel ID
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To fill in all channel IDs for the same cell type
STEP 1. Press All button.
STEP 2. Enter the desired name (or cell type and press OK button.
STEP 3. Confirm that the ID has automatically populated for all 4 channels,
then press Back button.
Channel ID
JurkatT All
JurkatT_1
JurkatT_2
JurkatT_3
JurkatT_4
Channel ID
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Using accessory input devices: barcode scanner, USB keypad or USB keyboard
(optional)
Keypad and barcode scanner are optional. Contact your local distributor if
required.
Connect the input device to the USB port on the back side of the device. When
properly
connected and recognized, an icon appears on the status bar.
Input
Device
Usage
Keypad
1. Enter a channel ID and press the “Enter” key
2. The cursor moves to the next channel ID box
(The direction key can also be used to move the cursor.)
Barcode
Scanner
1. Scan a barcode containing channel ID name.
2. The Channel ID box is filled with corresponding ID name, and the cursor
moves to the
next box when entered successfully.
STEP 1. Connect the keypad or barcode scanner via the USB port at the back
side of the QuadCount.
Check if icon is present at the top. Press the Barcode scanner button above
the channel
ID boxes.
Channel ID
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STEP 2. Touch the top blank text box and enter 4 channel IDs using the
connected keypad or the
barcode scanner (refer to the above table). The maximum channel ID length is
20 alphanumeric
characters or some special characters.
STEP 3. Confirm that up to 4 channel IDs boxes are correctly filled, then
press the Back button.
Channel ID
JurkatT
NIH
Hela
U937
Channel ID
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The Result screen
The following operations are performed on the result screen after counting.
After completing the cell counts, histograms of cell size distribution and
result images are provided.
While viewing histogram, it is possible to modify cell size gating parameters.
The QuadCount™ can
generate both histograms for individual channels and also a combined histogram
of all channels.
The QuadCount™ can detect 5 ~ 60µm diameter objects. However, the gating
system is set
by default to count from 8µm because most common cell lines have a size
starting at or
above 8µm.
NOTE: If you want to count cells smaller than 8 µm, change the cell size
gating parameter in the
histogram.
Toggle between the histogram and result image after selecting a channel.
12 – 34 19
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▪ Press or button to see the result images of selected channels.
▪ Return to default : The changed settings return to the default settings.
▪ Create preset : The adjusted settings can be saved as a new preset.
▪ Save in the current preset : The changed settings can be saved in current
preset (This is
not available in a fixed preset).
▪ Apply all : The changed settings is applied to all channels.
A. Analyzing by Histogram
STEP 1. Press a channel number to check, and switch to the histogram icon.
STEP 2. Press All to view the average data of all channels.
12 – 34 19
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STEP 3. Move both columns and adjust the cell size gating.
STEP 4. Check the results table of total cells, quantity of live, and
viability %.
12 – 26 19
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B. View Results Images
The QuadCount™ provides the results images after counting. One or more images
are acquired and
analyzed per channel, and the number of images depends on the count mode
selected. The “Result
image” screen shows the analyzed images with live cells circled in green and
dead cells circled in red.
STEP 1. Press a channel which you want to check, and switch to the Image icon.
STEP 2. Adjust Live / Dead cell definition.
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STEP 3. Press Data icon.
STEP 4. Review the number of Live cells and Viability %.
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C. Printout Cell Count Results using a Thermal Printer
The QuadCount™ can use a thermal printer to printout the counting result.
The Thermal printer is optional. Contact Accuris Instruments or your local
distributor for
ordering information.
Step 1. Connect the thermal printer to the USB port at the back side of the
device.
Confirm that the icon is present on the status bar, indicating that it is
recognized.
Press Print button.
Example
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D. Exporting a Report to a USB memory stick
A report of the counting results can be exported as a PDF to a USB memory
stick. The PDF report
shows general information, the cell image and the histogram of cell size
distribution.
Please use the USB memory stick included with the QuadCount™ or another that
is
formatted to FAT32 or NTFS file system. USB memory sticks formatted to the ex-
FAT file
system is not supported.
If an ex-FAT File system memory stick is connected, the USB memory icon will
display, but an
error message “Unsupported USB memory” will appear when trying to export data
or a
report.
Step 1. Connect the USB memory stick to the USB port on the back side of the
QuadCount.
Confirm that the icon is present on the status bar, indicating that it is
recognized.
Press the Export PDF button.
Step 2. A progress dialog box appears to indicate that exporting the report is
in progress.
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Step 3. Once the progress dialog box disappears and the notification message
(“Export success”) is
displayed on status bar, you can remove the USB memory stick from USB port.
NOTE: If the USB memory stick is removed before the “exporting” message
disappears, the results
file may be corrupted.
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E. Exporting Data (all history) to a USB memory stick
The results, recorded in a current user group (All history), can be exported
to a USB memory stick.
Results data are saved automatically in the device memory of activated user
group. By using the
“Exporting Data” feature, the data are exported as CSV file (comma-separated-
value format) which
can be opened by Microsoft Excel.
Please use the USB memory stick included with the QuadCount™ or another that
is
formatted to FAT32 or NTFS file system. USB memory sticks formatted to the ex-
FAT file
system is not supported.
If an ex-FAT File system memory stick is connected, the USB memory icon will
display, but an
error message “Unsupported USB memory” will appear when trying to export data
or a
report.
The QuadCount™ automatically saves data up to 1000 records per each of groups.
Step 1. Select the User group.
Step 2. Press Review.
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Step 3. The auto-saved results are displayed for the selected user group.
Connect a USB Memory stick to the USB port at the back side of the instrument.
Confirm that the icon is present on the status bar, indicating it is
recognized.
Press Export CSV button.
Step 4. A progress dialog box appears to indicate that exporting data is in
progress.
CSV data is now exporting…
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Step 5. Once the progress dialog box disappears and the notification message
“Exported all data” is
displayed on status bar, remove the USB memory from USB port.
NOTE: If the USB memory stick is removed before the “data is exporting”
message disappears, the
results file may be corrupted.
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F. Showing channel ID names
Step 1. To see each of Channel ID names, press Channel ID.
To go back to the channel numbers, press Back.
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Setting screen
FN
48
A. Checking Firmware information and Updating Firmware
Step 1. Press F/W info & Update, and connect a USB memory stick that contains
the appropriate
firmware update files.
Step 2. Choose the firmware category to update (Main or Display).
If USB the memory stick is not connected or it does not contain the update
program files, a message
will be displayed.
Step 3. Press Update button.
Step 4. Updating
Current version : 1.0
New version : 1.01
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Step 5. The device will restart automatically with updated firmware version.
Confirm that the version(s) have updated properly.
Step 6. After about 1 minute and the initialization is complete, switch the
power to off and then back
on again for stable operation.
NOTE: When the following message “Please wait…” appears on the initialization
screen after the
firmware update, please wait 2~3 minutes. Do not turn the device off the
device immediately.
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B. Bead Quality Control (Refer to the instructions included with the Bead QC
kit for additional
details.)
Step 1. Press the Bead QC button.
Step 2. Load a standard slide with the appropriate bead mixtures added to the
sample chambers
and press the START button.
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Step 3. Counting
Step 4. Check the resulting data.
Step 5. Check the Histogram and Bead image.
200 12 Check the image
STOP
12 – 34 12
Home
200 12 Check the image
320 15 Check the image
400 17 Check the image
350 19 Check the image
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Step 6. Return to Home screen.
C. Setting the Date and Time
STEP 1. Press Time button.
STEP 2. Adjust date and time accordingly.
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STEP 3. Press Set button to save the adjusted values.
STEP 4. Return to the Home screen.
22
54
Maintenance and Cleaning
The QuadCount™ instrument does not require regular maintenance or regular
replacement of
parts or components. Clean the external surface of the device using a soft
cloth. Isopropyl
alcohol or deionized water can be used together for cleaning the housing.
Do not allow cleaning liquids or solutions to enter the housing.
55
Appendix A. Trouble Shooting
Problem Cause Solution
Device
Not powering on
Power switch is in off position. Check the power switch on back of unit.
No power from outlet. Check the power source.
Bad power cable. Replace the cable.
Inaccurate result
Stain solution has expired or
been contaminated. Use new stain solution or filter the solution.
Too many aggregated cells.
Try again, pipette the cell mixture gently to mix the
cells before adding to the slide chambers.
(check the cell image for excessive cell clumping or
agglomerates)
Sampling error
✓ Repeat steps of properly pipetting the cell
mixture for the staining process.
✓ Before sampling the cell suspension, gently
resuspend the cells at least 6 times by gently
pipetting up and down
✓ Sample from the middle of the cell suspension
tube, not near the surface or the bottom.
Bubbles in slide chambers Pay careful attention to avoid bubbles when
pipetting and loading samples into the slide
low cell concentration
(≤5 x 104
)
Try again using the Precise mode.
Cell size is smaller than 10µm
or around 10µm.
Change the gating size parameter in the histogram.
The ratio of trypan blue in the
sample is too high or too low..
Mix cell suspension and trypan blue at a 1:1
volume ratio.
Too Bright or dark cell image
Mix cell suspension and trypan blue 1:1.
If the problem is not resolved, contact Accuris or
your local distributor.
The grid pattern or line is visible
in the result images.
Try again using another slide.
If the problem occurs frequently, contact your local
distributor.
Exported data or
Report is
corrupted
The USB memory was removed
before displaying the
notification message
Wait until after the notification message appears,
then remove the USB memory.
USB memory
Not Connected to
device
The USB memory is formatted
to ex-FAT or NTFS file system.
Use USB memory included with the QuadCount
package or another formatted to FAT32 file system
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If trypan blue or media is contaminated or contains any debris which is
similar in size and shape
to cells, this will causes inaccurate result.
Appendix B.
Examples of errors and inaccurate results
1. “Too Low” error
2. “Too High” error
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3. “Sample error”
Cells are severely aggregated The sample loaded into the slide has dried out
4. Contaminated stain solution
Cells mixed with contaminated trypan blue (Comparative image) Cells mixed with
filtered trypan blue
58
Appendix C. The contents of Results Data
exported as a .csv file:
History table (Excel data) consists of following items.
User Selected user group
File created Date and time when file was created
Channel No. Channel number
Channel ID Channel ID name
Date Measurement date
Time Measurement time
Total cell
[x10^4/mL] Total cell Count result
(x 1X104 cells/mL)
(Converted count result)
Live cell
[x10^4/mL] Live cell Count result
(x 1X104 cells/mL)
(Converted count result)
Dead cell
[x10^4/mL] Dead cell Count result
(x 1X104 cells/mL)
(Converted count result)
Viability Cell viability (%)
59
Appendix D.
Example and explanation of PDF report
60
All the materials in this manual are protected by US and international
copyright laws and cannot be
reproduced, translated, published or distributed without the permission of the
copyright owner.
QuadCountTM Instruction Manual
Website: http://www.accuris-usa.com
E-mail: info@accuris-usa.com
Accuris Instruments (a division of Benchmark Scientific)
PO Box 709
Edison, NJ 08818.
PH: 908.769.5555
FAX: 732.313.7007
The information in this manual is described as correctly as possible and is
applicable to the latest
firmware versions, but it may be changed without prior consent or
notification.
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