ACCURIS PR1400 Fast Extraction PCR Kit User Guide
- September 9, 2024
- ACCURIS
Table of Contents
ACCURIS PR1400 Fast Extraction PCR Kit
Specifications
-
Kit Type: Fast Extraction PCR
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Applications: End-point PCR, real-time PCR
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Chemistries: SYBR Green, TaqMan Probe
-
Package Contents:
-
Accuris Fast Extraction PCR Kit 100 reactions (Catalog number
PR1400-FE-100) -
Accuris Fast Extraction PCR Kit 400 reactions (Catalog number
PR1400-FE-400) -
Accuris Fast Extraction Lysis Solution 100 reactions (Catalognumber PR1400-FLS-100)
-
Accuris Fast Extraction Lysis Solution 400 reactions (Catalog number PR1400-FLS-400)
-
-
Quality Management: ISO 9001:2008 standards
Product Usage Instructions
General Guidelines
The genomic DNA prepared with this kit is ready for use in end-point PCR and
real-time PCR assays using SYBR Green or TaqMan Probe chemistries. Analysis
results can be analyzed using agarose gel electrophoresis.
Limitations of Use
For research purposes only. Not intended for therapeutic or diagnostic use.
Quality Control
Accuris enzymes and reagents are tested for activity, reproducibility,
efficiency, heat activation, sensitivity, and absence of nuclease
contamination and nuclease activity.
Reaction Setup
- Thaw Lysis Solution. Note: The Lysis Solution has a cloudy appearance.
- Add the PCR-ready DNA to the final reaction volume of 25uL.
- Perform the DNA extraction process in 0.2 mL PCR Tubes or 0.5 mL/1.5 mL microcentrifuge tubes in a thermal cycler or heating block.
- Follow the protocol provided in the manual for optimal results.
Frequently Asked Questions (FAQ)
Q: Can this kit be used for diagnostic purposes?
A: No, this kit is only intended for research purposes and not for diagnostic
use.
Accuris™ Fast Extraction PCR Kit
Description
The Accuris Fast Extraction PCR is engineered for the rapid, cost-efficient,
and effective extraction and amplification of DNA from a wide array of solid
tissues, such as mouse tails, ear punches, plant leaves, bacteria, and saliva.
This kit is composed of a unique single-tube extraction solution that enables
the release of genomic DNA in just 8 minutes. Following extraction, the PCR
amplification process utilizes the Accuris High Fidelity Hot Start Master Mix,
which is optimized for high-quality end-point PCR results. The inclusion of an
inert red dye in the High Fidelity Hot Start Master Mix facilitates the direct
loading of the PCR product onto a gel.
For enhanced versatility in laboratory applications, the genomic DNA prepared
with this kit is immediately ready for use in end-point PCR and real-time PCR
assays using SYBR Green or TaqMan Probe chemistries.
Upon receipt, immediately store at -20°C. Avoid excessive freeze/thaw cycles.
When stored as directed, this product will retain its activity for 12 months
from date of receipt. The product may also be stored at 4°C for up to one
month.
Limitations of Use
For research purposes only. Not intended for therapeutic or diagnostic use.
Quality Control
Accuris enzymes and reagents are tested under general assay conditions for
activity, reproducibility, efficiency, heat activation, sensitivity, and
absence of nuclease contamination and nuclease activity.
This product is manufactured under a comprehensive quality management system,
following ISO 9001:2008 standards.
General Guidelines
Template
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Tissue: Use 0.5 – 10 mg / 100uL Lysis
Solution -
Plant: Use 2.0 – 10 mg / 100uL Lysis
Solution -
Saliva: Use 10 – 20 uL / 100uL Lysis
Solution -
E. Coli : Use 1 Colony (0.5 – 2mm) /
100uL Lysis Solution
Reaction conditions
Most reactions can be carried out at a temperature of 42°C for 30 minutes.
When working with templates that have a high GC content (above 65%), the
temperature can be increased, up to 55°C, to alleviate any problems associated
with secondary structure.
Analysis
Results can be analyzed using agarose gel electrophoresis. The High Fidelity
Hot Start Master Mix contains an inert red tracking dye which migrates at the
rate of 600 bp and 350 bp DNA in 1% and 2% TAE agarose
gels, respectively.
Technical Support
For trouble-shooting and tech support, contact us at info@accuris-
usa.com or call 908 769-5555.
Accuris is not responsible for consequential or incidental damages, direct or
indirect, resulting from use of this product. Accuris guarantees the
performance of this product as described when used in accordance with these
instructions.
Reaction Setup
Thaw Lysis Solution. Note: The Lysis Solution has a cloudy appearance.
| Component | 25 µL reaction Final concentration |
|---|---|
| 2X HF Red Dye Mix | 12.5 µL |
| Forward Primer (10µM) Reverse Primer (10µM) | 1.0 µL |
1.0 µL
| 400 nm
400 nm
DNA Extract| 2.0 – 4.0uL|
PCR grade water| to 25uL final reaction volume|
Protocol
- The DNA extraction process can be carried out in 0.2 mL PCR Tubes or 0.5 mL/1.5 mL microcentrifuge tubes in a thermal cycler or heating block.
- Thaw Lysis Solution. Please note that the Lysis Solution has a cloudy appearance.
- Add your sample to a tube containing 100 µl of the Lysis Solution.
- Vortex the tube containing the sample and the lysis solution for 15 seconds.
- Transfer the tube to a heat block or a thermal cycler and prepare a DNA extract of PCR-ready DNA as follows:
| Cycles | Temperature & Time | Notes |
|---|---|---|
| 1 | 65°C, 6 minutes | Lysis |
| 1 | 98°C, 2 minutes | Protease Deactivation |
| 1 | 4°C | Cool on ice |
Package contents and reordering
Accuris Fast Extraction PCR Kit
100 reactions
Catalog number PR1400-FE-100
Accuris Fast Extraction PCR Kit
400 reactions
Catalog number PR1400-FE-400
Accuris Fast Extraction Lysis Solution 100 reactions
Catalog number PR1400-FLS-100
Accuris Fast Extraction Lysis Solution 100 reactions
Catalog number PR1400-FLS-400
Fast Extraction PCR Kit
- PR1400-FE-S PR1400-FE-100
- PR1400-FE-400 PR1400-FLS-S
- PR1400-FLS-100 PR1400-FLS-400tore at -20°C upon receipt.
: 908-769-5555 EM: info@accuris- usa.com