NanoTag Biotechnologies N1511 Alfa Selector Resins Instructions
- September 27, 2024
- NanoTag Biotechnologies
Table of Contents
N1511 Alfa Selector Resins
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Product Specifications
-
Product Name: ALFA Selector Resins
-
Epitope Tag: ALFA-tag (core sequence SRLEEELRRRLTE)
-
Compatibility: Compatible with physiological buffers,
Lysis/Washing buffers, high stringency buffers, detergents, and
reducing agents -
Available Variants: Non-magnetic and magnetic
Product Usage Instructions
1. Analytical Scale Purifications using Non-Magnetic ALFA
Selector Resins
-
Prepare cell lysates (0.2 to 1.5 ml volume) according to
established protocols. -
Add the prepared cell lysate to the equilibrated ALFA Selector
Resin. -
Incubate the mixture for binding.
-
Wash the resin to remove unbound proteins.
-
Elute the ALFA-tagged target proteins using either Acidic
Elution or Denaturing elution methods.
Elution Procedures:
a. Peptide Elution Batch Mode (ALFA SelectorPE / ALFA
SelectorCE)
-
Prepare a 100x (20 mM) stock solution of ALFA elution
peptide. -
Dilute the ALFA peptide stock solution in physiological buffer
to obtain an Elution buffer. -
Add the Elution buffer to the resin and incubate with
shaking. -
Collect the eluate by centrifugation after the specified
incubation time.
Acidic Elution: Use 0.1 M Glycine/HCl pH 2.2,
150 mM NaCl for efficient elution at low pH.
Denaturing Elution: Utilize SDS sample buffer
at elevated temperatures for efficient elution.
Frequently Asked Questions (FAQ)
Q: What are the recommended Mini Spin Columns for analytical
scale purifications?
A: We recommend using Mini Spin Columns (Cat. No. A1001) for
analytical scale immunoprecipitation experiments using non-magnetic
ALFA Selector Resins.
Q: How can I contact NanoTag Biotechnologies for more
information?
A: You can reach NanoTag Biotechnologies at Phone: +49 551
50556-365, E-mail: info@nano-tag.com, or visit their website at
www.nano-tag.com.
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ALFA Selector Resins
Immunoprecipitation of fusion proteins from cell extracts using
ALFA Selector Resins
The ALFA-tag is a novel, highly versatile epitope tag (core sequence
SRLEEELRRRLTE) that can be employed for a wide range of life science
applications(1). For immunoprecipitation of ALFA-tagged target proteins,
NanoTag Biotechnologies offers ALFA Selector Resins(1,2) based on different
nanobody variants.
· ALFA SelectorST (for Super Tight) is designed to reach the highest possible
binding strength. It features a nanobody binding ALFA-tagged targets with an
affinity of ~26 pM(1). Efficient elution of target proteins from ALFA
SelectorST requires acidic or denaturing conditions.
· ALFA SelectorPE (for Peptide Elution) displays a nanobody with lower
affinity for ALFA-tagged targets (Kd ~11 nM) and is optimized to allow for
competitive peptide elution under physiological conditions(1).
· ALFA SelectorCE (for Cold Elution) displays a nanobody with lower affinity
for ALFA-tagged targets (Kd ~100 nM). The resin is ideal for competitive
elution using physiological buffers at low temperature (i.e. 4°C) and can also
be used for flow elution at room temperature(2).
All ALFA Selector variants are available based on non-magnetic or magnetic 4%
agarose beads. The sitedirected and chemically stable immobilization of
nanobodies ensures a high capacity (>150 µM target protein, e.g., 4.5 mg GFP-
ALFA per ml of resin) and optimal accessibility of the available binding
sites. At the same time, minimal leakage of nanobodies is observed also under
harsh denaturing and/or reducing conditions. In contrast to conventional
immunoprecipitations, the eluates of both ALFA Selectors will not contain
large amounts of co-eluted antibody fragments. Due to the combination of high
capacity, minimal leakage and extraordinary low non-specific protein
adsorption, all ALFA Selector Resins allow for clean and highly specific
immunoprecipitations.
NanoTag’s ALFA Selector Resins are widely compatible not only with
physiological buffers and most common Lysis/Washing buffers, but also with
high stringency buffers, detergents and reducing agents (see compatibility
chart on page 8). The resins can therefore be used for customized applications
using a wide variety of buffer compositions and pH values.
Relevant products
ALFA SelectorST 2000 µl 50% slurry
ALFA SelectorPE 2000 µl 50% slurry including 10 mg ALFA elution peptide
ALFA SelectorCE 2000 µl 50% slurry including 10 mg ALFA elution peptide
ALFA elution peptide
4% crosslinked agarose Cat. No. N1511 4% magnetic agarose Cat. No. N1516
4% crosslinked agarose Cat. No. N1510 4% magnetic agarose Cat. No. N1515
4% crosslinked agarose Cat. No. N1512 4% magnetic agarose Cat. No. N1517
10 mg ALFA elution peptide (lyophilized) Cat. No. N1520
Materials (not included):
· Buffers: Lysis buffer · Washing buffer · Elution buffer · Tris-buffered
saline (TBS) pH 7.4 · 2x SDS sample buffer · Columns and tubes · Optional:
Magnetic rack
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
I. Analytical scale purifications using non-magnetic ALFA Selector Resins
We recommend using Mini Spin Columns (Cat. No. A1001) for analytical scale
immunoprecipitation experiments using non-magnetic ALFA Selector Resins
1. Prepare cell lysates (0.2 to 1.5 ml volume) according to established
protocols. For mammalian cells, we recommend using 106-108 cells per
experiment.
2. Clear lysate by centrifugation for 10 min at > 14000 x g and 4°C. Take
sample for further analysis (Input fraction).
3. Equilibrate ALFA Selector Resin: a. Resuspend ALFA Selector Resin. b.
Transfer 20 µl slurry (10 µl packed beads) into a clean 1.5 ml reaction tube.
c. Add 1 ml Lysis buffer. d. Centrifuge for 1 min at 1000 x g and carefully
remove supernatant. e. Repeat steps 3c-3d once.
4. Add cleared lysate from step 2 to the equilibrated ALFA Selector Resin
from step 3.
5. Incubate 1 h at 4°C with head-over-tail rotation.
6. Sediment beads by centrifugation for 1 min at 1000 x g and 4°C. Take
sample from supernatant for further analysis (Non-bound fraction).
7. Washing: a. Carefully remove supernatant. b. Resuspend beads in 1 ml Lysis
buffer. c. Centrifuge for 1 min at 1000 x g. d. Remove supernatant. e. Repeat
steps 7b-7d twice.
8. Transfer: a. Remove bottom plug from Mini Spin Column. Place column in 2
ml reaction tube. b. Resuspend beads in 200 µl Lysis buffer and transfer
suspension to Mini Spin Column. c. Wash out remaining beads sticking to the
tube with 200 µl Lysis buffer. Transfer the suspension to the Mini Spin
Column. d. Centrifuge for 1 min at 1000 x g and discard flow-through.
9. Washing: a. Add 400 µl Lysis buffer. b. Centrifuge for 1 min at 1000 x g
and discard flow-through. c. Repeat steps 9a-9b once.
10. Wash once with 400 µl TBS, centrifuge for 1 min at 1000 x g.
11. Attach bottom plug and place Mini Spin Column in a clean 1.5 ml reaction
tube.
12. Elution: Peptide elution under native conditions (ALFA SelectorPE and
ALFA SelectorCE). This elution mode is based on competition between the ALFA
elution peptide present in the Elution buffer and the ALFA-tagged target
protein for available binding sites on the resin. To obtain convenient elution
kinetics, peptide elution from ALFA SelectorPE has to be performed at room
temperature (22-25°C). ALFA SelectorCE can efficiently be eluted at
temperatures between 4 and 25°C. An enhanced elution kinetics is observed at
even higher temperatures (e.g. 37°C).
Acidic elution (all ALFA Selector Resins). ALFA-tagged target proteins can
efficiently be eluted from all ALFA Selector variants at low pH. As a general
Acidic Elution Buffer, we recommend 0.1 M Glycine/HCl pH 2.2, 150 mM NaCl.
Denaturing elution using SDS sample buffer (all ALFA Selector Resins). ALFA-
tagged target proteins can efficiently be eluted from both ALFA Selector
variants using SDS sample buffer at elevated temperatures.
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
I. Analytical scale purifications using non-magnetic ALFA Selector Resins (continued)
Elution procedures:
a. Peptide elution batch mode (ALFA SelectorPE / ALFA SelectorCE).
i. Prepare a 100x (20 mM) stock solution of ALFA elution peptide by solubilizing the ALFA elu-
tion peptide at 40 mg/ml in deionized water.
ii. Dilute the ALFA peptide stock solution 1:100 in physiological buffer to obtain an Elution
buffer containing 200 µM ALFA elution peptide.
iii. Attach bottom plug on column. Add 5 resin volumes (RV) of Elution buffer to the resin.
iv. Incubate with subtle shaking:
ALFA SelectorPE:
15-20 min at room temperature.
ALFA SelectorCE:
3-5 min at room temperature.
or 15-20 min at 4°C.
v. Remove the bottom plug and collect the eluate by centrifugation for 1 min at 1000 x g.
vi. Optional: For highest yields attach bottom plug and repeat steps iii-v. Combine eluates.
b. Elution under acidic conditions (all ALFA Selector variants).
Please check the general remarks related to acidic elution approaches on page
8.
i. Prepare Acidic Elution Buffer (e.g. 0.1 M Glycine/HCl pH 2.2, 150mM NaCl).
ii. Add 2.5 resin volumes (RV) of Acidic Elution Buffer to the resin. iii.
Incubate for 2 min at RT with subtle shaking. iv. Remove the bottom plug and
collect the eluate by centrifugation 1 min at 1000 x g. v. Attach bottom plug
and repeat steps ii-iv once. vi. Combine eluates. vii. Neutralize eluates by
adding e.g. 1/10 volume of 1 M Tris-HCl pH 8.5.
c. Denaturing elution using SDS sample buffer (all ALFA Selector variants).
Denaturing elution will in general not be compatible with protein
functionality. It is therefore explicitly recommended for analytical
applications only.
i. Add 2.5 resin volumes (RV) of SDS sample buffer pre-warmed to ~60°C to the
resin. ii. Close the Mini Spin Column by attaching the lid and bottom plug.
iii. Incubate for 5 min at 80-90°C with subtle shaking. iv. Open lid and
remove the bottom plug and collect the eluate by centrifugation 1 min at
1000 x g. v. Attach bottom plug and repeat steps ii-iv once. vi. Combine
eluates.
13. Take sample from elution fractions for further analysis (Elution
fraction).
14. Analyze collected samples by SDS-PAGE.
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
II. Analytical scale purifications using magnetic ALFA Selector Resins
1. Prepare cell lysates (0.2 to 1.5 ml volume) according to established
protocols. For mammalian cells, we recommend using 106-108 cells per
experiment.
2. Clear lysate by centrifugation for 10 min at > 14000 x g and 4°C. Take
sample for further analysis (Input fraction).
3. Equilibrate magnetic ALFA Selector Resin: a. Resuspend ALFA Selector
Resin. b. Transfer 20 µl slurry (10 µl packed beads) into a clean 1.5 ml
reaction tube. c. Add 1 ml Lysis buffer. d. Collect beads by placing the tube
in a magnetic rack and remove supernatant. e. Repeat steps 3c-3d once.
4. Add cleared lysate from step 2 to the equilibrated magnetic ALFA Selector
Resin from step 3.
5. Incubate 1 h at 4°C with head-over-tail rotation.
6. Collect beads by placing the tube in a magnetic rack. Take sample from
supernatant for further analysis (Non-bound fraction).
7. Washing: a. Carefully remove supernatant. b. Resuspend beads in 1 ml Lysis
buffer and incubate 1-2 min head-over-tail. c. Collect beads by placing the
tube in a magnetic rack. d. Remove supernatant. e. Repeat steps 7b-7d three
times. f. Resuspend beads in 1 ml Lysis buffer and transfer suspension into
fresh 1.5 ml tube. Note: This step is essential as generally a lot of
contaminant proteins stick to the walls of the tube used during the binding
step! g. Wash beads twice as described in steps 7b-7d. h. Wash once with 1 ml
TBS and collect beads on magnetic rack. i. Remove the supernatant completely.
12. Elution: Peptide elution under native conditions (ALFA SelectorPE and
ALFA SelectorCE). This elution mode is based on competition between the ALFA
elution peptide present in the Elution buffer and the ALFA-tagged target
protein for available binding sites on the resin. To obtain convenient elution
kinetics, peptide elution from ALFA SelectorPE has to be performed at room
temperature (22-25°C). ALFA SelectorCE can efficiently be eluted at
temperatures between 4 and 25°C. Enhanced elution kinetics are observed at
even higher temperatures (e.g. 37°C). Acidic elution (all ALFA Selector
Resins). ALFA-tagged target proteins can efficiently be eluted from all ALFA
Selector variants at low pH. As a general Acidic Elution Buffer we recommend
0.1 M Glycine/HCl pH 2.2, 150 mM NaCl. Denaturing elution using SDS sample
buffer (all ALFA Selector Resins). ALFA-tagged target proteins can efficiently
be eluted from both ALFA Selector variants using SDS sample buffer at elevated
temperatures.
(Protocol continued on next page)
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
II. Analytical scale purifications using magnetic ALFA Selector Resins (continued)
Elution procedures:
a. Peptide elution batch mode (ALFA SelectorPE / ALFA SelectorCE).
i. Prepare a 100x (20 mM) stock solution of ALFA elution peptide by solubilizing the ALFA elu-
tion peptide at 40 mg/ml in deionized water.
ii. Dilute the ALFA peptide stock solution 1:100 in physiological buffer to obtain an Elution
buffer containing 200 µM ALFA elution peptide.
iii. Add 5 resin volumes (RV) Elution buffer to the resin.
iv. Incubate with subtle shaking:
ALFA SelectorPE:
15-20 min at room temperature.
ALFA SelectorCE:
3-5 min at room temperature.
or 15-20 min at 4°C.
v. Sediment the beads by placing on a magnetic rack and collect the eluate (= supernatant).
vi. Optional: For highest yields repeat steps iii-v. Combine the eluates.
b. Elution under acidic conditions (all ALFA Selector variants).
Please check the general remarks related to acidic elution approaches on page
8.
i. Prepare Acidic Elution Buffer (e.g. 0.1 M Glycine/HCl pH 2.2, 150mM NaCl).
ii. Add 2.5 resin volumes (RV) of Acidic Elution Buffer to the resin. iii.
Incubate for 2 min at RT with subtle shaking. iv. Sediment the beads by
placing on a magnetic rack and collect the eluate (= supernatant). v. Repeat
steps ii-iv once. vi. Combine eluates. vii. Neutralize eluates by adding e.g.
1/10 volume 1 M Tris-HCl pH 8.5.
c. Denaturing elution using SDS sample buffer (all ALFA Selector variants).
Denaturing elution will in general not be compatible with protein
functionality. It is therefore explicitly recommended for analytical
applications only.
i. Add 2.5 resin volumes (RV) of 2x SDS sample buffer pre-warmed to ~60°C to
the resin. ii. Incubate for 5 min at 80-90°C with subtle shaking. iii.
Sediment the beads by placing on a magnetic rack and collect the eluate (=
supernatant). iv. Repeat steps i-iii once. v. Combine eluates.
13. Take sample from elution fractions for further analysis (Elution
fraction).
14. Analyze collected samples by SDS-PAGE.
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
III. Preparative purifications using peptide elution in flow or stopped flow mode
Notes:
Peptide elution is based on competition between the ALFA elution peptide
present in the Elution buffer and the ALFA-tagged target protein for available
binding sites on the resin. Peptide elution in flow mode generally requires a
fast release of target proteins, which can most efficiently be assured when
eluting ALFA SelectorCE at room temperature. Flow elution from ALFA SelectorCE
at 4°C is possible but will generally require flow rates <0.05 resin volumes
(RV)/min(2).
·
Procedure:
1. Prepare native cell lysates according to established protocols.
2. Clear lysate by centrifugation for 10 min at > 14000 x g and 4°C.
3. Equilibrate ALFA SelectorCE: a. Resuspend ALFA SelectorCE. b. Transfer the
slurry to a clean flow column. c. Wash resin with at least 5 resin volumes
(RV) of Lysis Buffer.
4. Binding:
a. Binding in batch mode:
i. Add equilibrated ALFA Selector Resin (step 3) to the cleared lysate (step
2).
ii. Incubate 1 h at 4°C with rotation. iii. Sediment beads by centrifugation
for 1 min
at 1000 x g and 4°C. iv. Discard the supernatant and transfer beads
into the empty flow column used in step 3.
b. Binding in flow mode:
i. Slowly pass the cleared lysate obtained in step 2 over the equilibrated
column, collect flow-through.
ii. Optional: Pass non-bound material (flowthrough) once more over the column.
5. Wash resin with 10-20 RV of Washing buffer.
6. Elution:
a. Prepare a 100x (20 mM) stock solution of ALFA elution peptide by
solubilizing the ALFA elution peptide at 40 mg/ml in deionized water.
b. Dilute the ALFA peptide stock solution 1:40 in physiological buffer to
obtain an Elution buffer containing 500 µM ALFA elution peptide.
c. Elute column in flow mode at a flow rate of 0.1 to 0.2 RV/min (room
temperature) or <0.05 RV/min at 4°C.
Note: When using gravity flow columns, the elution speed can be adjusted by
adding buffer aliquots at defined time intervals: E.g. to achieve an effective
flow rate of 0.1 RV/min, add 0.2 RV of Elution buffer every 2 minutes and
collect the eluate correspondingly. To lower the net flow rate, increase the
intervals between each addition of Elution buffer.
7. Analyze input material, non-bound material and eluate fractions by SDS- PAGE.
Notes:
· A slightly enhanced elution efficiency can generally be observed when
increasing the peptide concentration to 1 mM(2). Even higher peptide
concentrations will generally not result in sharper elution profiles.
· The highest target protein concentrations can be achieved at low elution
speed(2). · Target proteins displaying a single ALFA-tag are typically eluted
within 1-2 resin volumes(2).
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
IV. Preparative purifications using acidic elution in flow or stopped flow mode
Please check the general remarks related to acidic elution approaches on page 8.
1. Prepare native cell lysates according to established protocols.
2. Clear lysate by centrifugation for 10 min at > 14000 x g and 4°C.
3. Equilibrate ALFA Selector: a. Resuspend ALFA Selector. b. Transfer the slurry to a clean flow column. c. Wash resin with at least 5 resin volumes (RV) of Lysis buffer.
4. Binding:
a. Binding in batch mode:
i. Add equilibrated ALFA Selector Resin (step 3) to the cleared lysate (step
2).
ii. Incubate 1 h at 4°C with rotation. iii. Sediment beads by centrifugation
for 1 min
at 1000 x g and 4°C. iv. Discard the supernatant and transfer beads
into the empty flow column used in step 3.
b. Binding in flow mode:
i. Slowly pass the cleared lysate obtained in step 2 over the equilibrated
column, collect flow-through.
ii. Optional: Pass non-bound material (flowthrough) once more over the column.
5. Wash resin with 10-20 RV of Washing buffer.
6. Elution:
a. Elute the column using an Acidic Elution Buffer at a flow rate of 0.2-0.5
RV/min. b. Immediately neutralize all eluate fractions e.g. by adding 1/10
volume of 1M Tris pH 8.5.
Note: When using gravity flow columns, the elution speed can be adjusted by
adding buffer aliquots at defined time intervals: E.g. to achieve a net flow
rate of 0.2 RV/min, add 0.2 RV of Elution buffer every minute and collect the
eluate correspondingly. To lower the net flow rate, increase the intervals
between each addition of Acidic Elution Buffer.
7. Analyze input material, non-bound material and eluate fractions by SDS- PAGE
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
V. Buffer compatibility (1,2)
Please note that the buffer compatibility of ALFA Selector Resins differs for
binding and washing steps, respectively. In general, ALFA Selector Resins are
less sensitive when charged with an ALFA-tagged target protein. Therefore, it
is possible to perform highly stringent washing steps under conditions
incompatible with an initial binding. Not all possible combinations of
reagents have been explicitly tested. It is likely that certain combinations
of reagents show more than additive effects. For example, such non-linear
effects can be expected when combining denaturing agents (including denaturing
detergents like SDS) with high concentrations of reducing agents.
A. Compatibility during binding
Class Salt Denaturing agents Detergents
Reducing agents pH value(a) Other substances
Reagent NaCl MgSO4 Urea Guanidin-HCl Triton-X 100 DDM CHAPS Desoxycholate SDS DTT
ALFA SelectorST >3 M >1 M 1.5 M 0.3 M 0.5 % 0.5 % 0.5 % 0.1 %
not compatible >100 mM 6.5 8.0
ALFA SelectorPE >3 M >1 M 1.5 M 0.3 M 0.5 % 0.5 % 0.5 % 0.1 %
not compatible >100 mM 6.5 8.0 not tested
ALFA SelectorCE >3 M 0.8 M 1.5 M 0.3 M 0.5 % 0.5 % 0.5 % 0.1 %
not compatible >100 mM 6.5 8.0
(a) We generally recommended binding at physiological pH. Binding beyond the given pH boundaries has to be tested on a case-to-case basis.
B. Compatibility during washing
Class Salt Denaturing agents Detergents
Reducing agents pH value Other substances
Reagent NaCl MgSO4 Urea Guanidin-HCl Triton-X 100 DDM CHAPS Desoxycholate SDS DTT
ALFA SelectorST >3 M >1 M 6 M >2 M >1 % >1 % >1 % 0.5 % 0.1 %(b)
100 mM 5 10
ALFA SelectorPE >3 M >1 M 3 M 2 M >1 % >1 % >1 % 0.5 %
not compatible >100 mM 5 10 not tested
ALFA SelectorCE >3 M 0.8 M 1.5 M 0.3 M 0.8 % 0.8 % 0.8 % 0.1 %
not compatible >100 mM 6 10
(b) Time- and temperature-dependent partial leakage. Not recommended for prolonged washing steps.
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
ALFA Selector Resins
VI. Specific notes on acidic elution procedures
· Acidic elution can be performed with all ALFA Selector variants at room temperature or at 4°C. The ALFA Selector variants display slightly different sensitivity towards low pH (see table below).
Selector Variant Stable binding
Efficient elution
ALFA SelectorST ALFA SelectorPE ALFA SelectorCE
pH 5 to pH 10 pH 5 to pH 10 pH 6 to pH 10
pH 2.2 pH 3 or below pH 4 or below
· An efficient elution from all ALFA Selector variants can generally be
achieved using an Acidic Elution Buffer containing 0.1 M Glycine/HCl pH 2.2,
150 mM NaCl.
· Not all target proteins will tolerate low pH values. It is therefore
important to adjust the elution conditions to the target protein of choice.
· It may be required to optimize the flow rate/incubation time, temperature
and pH value in order to maintain the proper folding/activity of the target
protein and/or the integrity of purified protein complexes.
VII. References
(1) Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A,
Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. The ALFA-
tag is a highly versatile tool for nanobody-based bioscience applications. Nat
Commun. 2019 Sep 27;10(1):4403.
(2) Kilisch M, Götzke H, Gere-Becker M, Crauel A, Opazo F, Frey S. Discovery
and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for
Protein Purification at Low Temperatures in Physiological Buffer.
Biomolecules. 2021 Feb 12;11(2):269.
All ALFA Selector Resins are only for research applications, not for
diagnostic or therapeutic use!
For further information concerning this protocol please contact us at info
@nano-tag.com
NanoTag Biotechnologies GmbH, Rudolf-Wissell-Str. 28a, 37079 Göttingen, Germany
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Phone: +49 551 50556-365, E-mail: info@nano-tag.com, Web: www.nano-tag.com
References
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