dynamic BIOSENSORS HK-FC-3 FC Capture A/G Kit User Manual

: September 6, 2024
: dynamic BIOSENSORS

Table of Contents

dynamic BIOSENSORS HK-FC-3 FC Capture A/G Kit
Key Features
Helix Adapter Chip Overview
Product Description
Workflow of a heliX FC – -capture assay
Preparation
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

dynamic BIOSENSORS HK-FC-3 FC Capture A/G Kit

dynamic-BIOSENSORS-HK-FC-3 FC-Capture-A-G-Kit-PRODUCT

Key Features

This kit is designed for the capture of IgG molecules binding to Protein A and G.
Compatible with heliX Adapter Chip.
Includes Adapter strands and Ligand strands modified with Protein A/G for 20 regenerations.
For functionalization of Spot 1 and Spot 2.
Adapter strands 1 and 2 carry a moderately hydrophilic red dye (Ra) with a single positive net charge.

Helix Adapter Chip Overview

$dynamic-BIOSENSORS-HK-FC-3 FC-Capture-A-G-Kit-FIG- $1$$

2 spots with 2 different anchor sequences for DNA-encoded addressing.
Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference.

Product Description

Order Number: HK-FC-3
Table 1. Contents and Storage Information

$dynamic-BIOSENSORS-HK-FC-3 FC-Capture-A-G-Kit-FIG- $2$$

For research use only. This product has a limited shelf life, please see the expiry date on the label. After the preparation of the ready-to-use solution, the expiry date is 6 months. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G.

Workflow of a heliX FC – -capture assay

$dynamic-BIOSENSORS-HK-FC-3 FC-Capture-A-G-Kit-FIG- $3$$

The anchor strand (ssDNA) immobilized to the surface of the heliX Adapter Chip is hybridized with complementary DNA strands modified with Protein A/G.
The IgG of interest is captured on the surface during the measurement run.
Measurement of the analyte binding kinetics.
Surface regeneration by injection of a high pH solution. The chip surface goes back to its original state. This step can be followed by a new hybridization of fresh ligands with Protein A/G.

Preparation

Mix 100 µL Protein A/G – Ligand strand with 100 µL Adapter strand 1 – Ra.
Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
Mix 200 µL Adapter strand 2 – Ra – lfs to the sample after step 2.
The solution (400 µL in total) is ready to use for biochip functionalization.
Please aliquot and store the ready-to-use solution at 2-8°C. Use up within 6 months.
The kit contains material for the preparation of two separate ready-to-use solutions with 400 µL each.

Assay Setup in heliOS
Go to heliOS > create a New Assay Workflow > add Custom Assay > load Capture with Kinetics > modify the parameters based on your needs and run the assay. Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.
TIP If the fluorescent change signal upon analyte binding is very small, consider using the conjugation approach, to move the binding site closer to the dye. For further questions, please contact the support team at support @dynamic-biosensors.com

Useful Order Numbers

Table 2. Order Numbers

$dynamic-BIOSENSORS-HK-FC-3 FC-Capture-A-G-Kit-FIG- $4$$