dynamic BIOSENSORS AS-2-Ga-lfs v5.1 Adapter Strand User Manual
- September 6, 2024
- dynamic BIOSENSORS
Table of Contents
AS-2-Ga-lfs v5.1 Adapter Strand
Product Information
Specifications
- Product Name: heliX+
- Model: ADAPTER STRAND 2 with green dye Ga
- Prehybridized with ligand strand
- Manufacturer: Dynamic Biosensors GmbH & Inc.
- Order Number: AS-2-Ga-lfs v5.1
- Key Features: 2 spots with 2 different anchor sequences for
DNA-encoded addressing
Product Description
The heliX+ ADAPTER STRAND 2 is a product prehybridized with a
ligand strand and features 2 spots with 2 different anchor
sequences for DNA-encoded addressing. It comes with green dye Ga
for easy visualization.
Contents and Storage Information:
-
Material: Adapter strand 2 – Ga – lfs
-
Cap Color: White
-
For research use only. Limited shelf life, check expiry date on
label -
To avoid freeze-thaw cycles, please aliquot the nanolever
-
Buffer: TE40
Product Usage Instructions
Preparation | MIX&RUN
-
Mix Adapter strand 1 – Ga (400 nM) and conjugated Ligand strand
(500 nM) at 1:1 ratio (v/v). -
Incubate the solution from step 1 at room temperature at 600
rpm for 30 minutes for complete hybridization. -
Mix the solution from step 2 with Adapter strand 2 – Ga – lfs
(200 nM) at 1:1 ratio (v/v). -
The solution is now ready for use in biochip
functionalization.
Note: The stability of the solution is
dependent on the ligand molecule’s stability.
FAQ
Q: Can the heliX+ ADAPTER STRAND 2 be used for multiple
experiments?
A: The product is designed for single-use
experiments due to its limited shelf life and stability
concerns.
Q: How should I store the heliX+ ADAPTER STRAND 2?
A: Store the product according to the storage
information provided in the manual, and avoid exposing it to
frequent freeze-thaw cycles.
heliX+
User Manual
ADAPTER STRAND 2
with green dye Ga and prehybridized with ligand strand
Dynamic Biosensors GmbH & Inc. AS-2-Ga-lfs v5.1
Key Features
· Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2. ·
Compatible with heliX® Adapter Chip. · Includes Adapter strands for 50
regenerations. · Ideal for MIX&RUN sample preparation. · Adapter strand 2
carries a hydrophilic green dye (Ga) with a single negative net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-2-Ga-lfs
Table 1. Contents and Storage Information Material Adapter strand 2 – Ga – lfs
Cap White
Concentration Amount 200/250 nM 5 x 200 µL
For research use only. This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolever.
Buffer TE40 [1]
Storage -20°C
AS-2-Ga-lfs v5.1
www.dynamic-biosensors.com
Page 1 of 3
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
1. Mix Adapter strand 1 – Ga (400 nM) and conjugated Ligand strand (500 nM)
at 1:1 ratio (v/v).
2. Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure
complete hybridization.
3. Mix solution of step 2 and Adapter strand 2 – Ga – lfs (200 nM) at 1:1
ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
Material
Adapter strand 1 – Ga
Ligand strand carrying the conjugated ligand
Concentration 400 nM 500 nM
Buffer TE40 [1] PE40 [
2]
Related Product Name Adapter strand 1 with green dye Ga heliX® Amine Coupling Kit 1
Order No AS-1-Ga HK-NHS-1
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM.
Vial 1
Adapter strand 1 – Ga (400 nM)
25 µL
Conjugated Ligand strand (500 nM)
25 µL
Vial 2
Adapter strand 2 – Ga – lfs (200/250 nM)
50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
AS-2-Ga-lfs v5.1
www.dynamic-biosensors.com
Page 2 of 3
Contact
Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany
Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA
Order Information order@dynamic-biosensors.com Technical Support support @dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2024
Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 M EDTA, 50 M EGTA [2] If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
AS-2-Ga-lfs v5.1
www.dynamic-biosensors.com
Page 3 of 3
References
Read User Manual Online (PDF format)
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