AffiGEN AffiVET PRRS Virus Antibody Rapid Test Instruction Manual

: July 3, 2024
: AffiGEN

Table of Contents

Material Required
Storage & Expiry Date
Test Principle
Result
Cautions
Contact us
Documents / Resources

PRRSV Antibody
AffiVET® Rapid Test Kit
AFG-VGS-60
40 Tests
AffiGEN AffiVET PRRS Virus Antibody Rapid Test

Material Required

(40) PRRSV Antibody Test Cards
(40) Droppers
(01) User Manual

Storage & Expiry Date

Store the test kit at 4 to 30°C in dark, sealed, dry place. DO NOT FREEZE.
The expiry date is 12 months from the production date, which is indicated on the box.

Test Principle

The AffiVET® Test Strip applies a colloidal gold immunochromatography technique for qualitative detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) antibody in porcine serum, plasma, and whole blood.
During testing, the PRRSV Antibody interacts with the antigen coated with colloidal gold, forming complexes that migrate across the strip..
Upon reaching the T-line, specific antigens on the membrane capture the complexes, resulting in the appearance of a T line.
The presence of both the Test line (T line) and the control line (C line) indicates a positive test result.

AffiGEN AffiVET PRRS Virus Antibody Rapid Test

Sample
Collection & Preparation

Whole blood

Collect anticoagulated whole blood.
Test the same day.

Serum

Collect blood and keep at 37℃ for 1-2h.
Take the supernatant and centrifuge at 1500r/min for 10min then separate the serum.
The serum can be stored for 2-3 days at 4℃.
For long term storage, freeze at -20°C.

Test Procedure

Read the manual carefully.
The test card and the sample need to be at room temperature before use.
Take out the test card.
se the dropper to absorb the sample.
Drop 2 to 3 drops into the well marked with “S”, or use Micropipette to transfer 70 µL of the sample into well mark “S”.
Read the results in 5 to 10 min at room temperature.
The result is invalid after 15 min.

Result

Interpretation

Negative Result

Presence of C-Line
Absence of T-Line

This means there is no PRRSV antibody in sample or the level is lower than the detection threshold.

Positive Result

Presence of C-Line
Presence of T-Line
This confirms the presence of PRRSV antibodies in sample

Invalid Result

Absence of C-Line
Presence of T-Line
Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions

The test card can only be used when brought to room temperature.
Don’t reuse the test card or use it after the expiry date.
Bring the sample and all the reagents to room temperature before use.
When the test card is unsealed, please use it as soon as possible.
Take precaution while preparing the sample. Gloves and mask are required.
The whole blood needs to be collected in anticoagulant tube.
Don’t freeze the whole blood.
The serum sample can be stored frozen but avoid repeated freezing and thawing.
This product is only for in vitro rapid diagnostic veterinary use.
This is a qualitative test. It is not quantitative.
The test results are for reference only. For confirmation, please refer to the relevant national standard methods.

Contact us

Phone 800-660-1620
Email info@affigen.com
Website www.affigen.com

https://affigen.com/

Documents / Resources

| AffiGEN AffiVET PRRS Virus Antibody Rapid Test [pdf] Instruction Manual
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