BRESSER Biolux SEL Student microscope with hard shell case Instruction Manual

June 4, 2024
BRESSER

BRESSER Biolux SEL Student microscope with hard shell case

Operating Instructions

WARNING! Not for children under three years. Choking hazard – small parts. Contains functional sharp edges and points! ATTENTION: Only suitable for children of at least 8 years of age. Instructions for parents or other responsible persons are enclosed and must be followed. Keep packaging as it contains important information.

General Warnings

  •  Choking hazard — This product contains small parts that could be swallowed by children. This poses a choking hazard.
  •  Risk of electric shock — This device contains electronic components that operate via a power source (batteries). Only use the device as described in the manual, otherwise you run the risk of an electric shock.
  •  Risk of fire/explosion — Do not expose the device to high temperatures. Use only the recommended batteries. Do not short-circuit the device or batteries, or throw them into a fire. Excessive heat or improper handling could trigger a short-circuit, a fire or an explosion.
  •  Risk of chemical burn — Make sure you insert the batteries correctly. Empty or damaged batteries could cause burns if they come into contact with the skin. If necessary, wear adequate gloves for protection.
  •  Leaking battery acid can lead to chemical burns. Avoid contact of battery acid with skin, eyes and mucous membranes. In the event of contact, rinse the affected region immediately with a plenty of water and seek medical attention.
  •  Use only the recommended batteries. Always replace weak or empty batteries with a new, complete set of batteries at full capacity. Do not use batteries from different brands or with different capacities. Remove the batteries from the unit if itis not to be used for a long period of time, or if the batteries are empty!
  •  Never recharge normal, non-rechargeable batteries. This could lead to explosion during the charging process.
  •  Rechargeable batteries are only to be charged under adult supervision.
  •  Rechargeable batteries are to be removed from the toy before being charged
  •  The terminals must not be short-circuited.
  •  Do not disassemble the device. In the event of a defect, please contact your dealer. The dealer will contact the Service Centre and can send the device in to be repaired, if necessary.
  •  Tools with sharp edges are often used when working with this device. Because there is a risk of injury from such tools, store this device and all tools and accessories in a location that is out of the reach of children.
  •  Keep instructions and packaging as they contain important information.
  •  The chemicals and liquids provided should be kept out of reach of children.
  • Do not drink the chemicals! Hands should be washed thoroughly under running water after use. In case of accidental contact with the eyes or mouth rinse with water. Seek medical treatment for ailments arising from contact with the chemical substances and take the chemicals with you to the doctor.
  • Check the Power supply for damage to the cord, plug, enclosure or other parts on a regular basis before use.
  • Do not use until the damage has been repaired. The toy is not intended for children under three years. The toy must only be used with the recommended power supply.
  • The power supply is not a toy. The toy is not to be connected to more than the recommended number of power supplies.
    • battery contains cadmium
    • battery contains mercury
    • battery contains lead

TIPS on cleaning

Remove the device from it’s energy source before cleaning (remove plug from socket / remove batteries). Clean the exterior of device with a dry cloth. Do not use cleaning fluids so as to avoid causing damage to electronic components. Toys liable to be cleaned with liquid are to be disconnected from the power supply before cleaning Clean the lens (objective and eyepiece) only with the cloth supplied or some other soft lintfree cloth (e.g. micro-fibre). Do not use excessive pressure – this may scratch the lens.

DISPOSAL

Dispose of the packaging material/s as legally required. Consult the local authority on the matter if necessary. Do not dispose of electrical equipment in your ordinary refuse. The European guideline 2002/96/EU on Electronic and Electrical Equipment Waste and relevant laws applying to it require such used equipment to be separately collected and recycled in an environment-friendly manner. Empty batteries and accumulators must be disposed of separately. Information on disposing of all such equipment made after 01 June 2006 can be obtained from your local authority. In accordance with the regulations concerning batteries and rechargeable batteries, disposing of them in normal household waste is explicitly forbidden. Please pay attention to dispose of your used batteries as required by law – at a local collection point or in the retail market (a disposal in domestic waste violates the Battery Directive). Batteries that contain toxins are marked with a sign and a chemical symbol. „Cd“ = cadmium, „Hg“ = mercury, „Pb“ = lead.

EC Declaration of Conformity

Bresser GmbH has issued a „Declaration of Conformity“ in accordance with applicable guidelines and corresponding standards. The full text of the EU declaration of conformity is available at the following internet address: www.bresser.de/download/8855600000000/CE/8855600000000_CE.pdf UKCA Declaration of Conformity Bresser GmbH has issued a „Declaration of Conformity“ in accordance with applicable guidelines and corresponding standards. The full text of the UKCA declaration of conformity is available at the following internet address: www.bresser.de/download/8855600000000/UKCA/8855600000000_UKCA.pdf

Bresser UK Ltd.

Suite 3G, Eden House, Enterprise Way, Edenbridge, Kent TN8 6HF, Great Britain

Here are the parts of your microscope

  1.  10x WF Eyepiece
  2.  20x WF Eyepiece
  3.  Barlow Lens
  4.  Eyepiece supports
  5.  Microscope Head
  6.  Objective Nosepiece
  7.  Objective
  8.  Clips
  9.  Microscope Stage
  10.  LED Illumination (transmitted light)
  11.  Microscope Base
  12.  Selection Knob for Illumination
  13.  Battery compartment
  14.  Focus knob
  15.  Color Filter wheel
  16.  LED Illumination (reflected light)
  17.  5 Slides, 5 Cover Sips and 5 Prepared Specimens plastic box
  18.  Specimens:
    • a) Yeast
    • b) Sea Salt
    • c) Shrimp Eggs
    • d) Empty Bottle
    • e) Empty Bottle
  19. Specimen slicer
  20.  Hatchery
  21.  Test tube
  22.  Tweezers
  23.  Dissecting needle
  24.  Dissecting knife
  25.  Protective cover
  26.  Container
  27.  Smartphone holder

How do I use my microscope?
Before you assemble your microscope, make sure that the table, desk or whatever surface that you want to place it on is stable, and does not wobble.

How do I operate the electric LED illumination?

In the base of the microscope there is a battery compartment (13). Loosen the screw at the battery compartment cover with a small Philips screwdriver and remove the cover. Place the batteries in the compartment so that the flat minus poles (-) press against the spring terminal and the plus poles (+) are touching the flat contact sheets. Close the battery compartment with the cover and turn the microscope around again. The first lamp shines onto the specimen from below and the second from above. (The thing that you want to observe with the microscope is called the object or specimen, by the way.) You can use each lamp on its own, or both of them together. There is a selection knob for this (12).

It has three numbers: I, II and III. If you select the

  • I, the light only comes from below (transmitted light).
  • II, the light only comes from above (reflected light).
  • III, both lamps shine light on the specimen

For transparent objects (transmitted-light objects), number I is best. In order to observe firm, non-transparent objects (direct-light objects), select number II. For semi-transparent objects, it is best to select number III. It is not recommended to use number III for transmitted-light objects on slides, since the light may cause reflections on the surface of the slide, which will disturb your observation.

When do I use the color filters?

The color filter wheel (15) is located below the microscope stage (9). They help you when you are observing very bright or clear specimens. Here, you can choose from various colors. This helps you better recognize the components of colorless or transparent objects (e.g. grains of starch, protozoa).

How do I adjust my microscope correctly?

Each observation starts with the lowest magnification. Adjust the microscope stage (9) so that it goesall the way down to the lowest position. Then, turn the objective nosepiece (6) until it clicks into place at the lowest magnification (objective 4x).

Note :
Before you change the objective setting, always move the microscope stage (9) to its lowest position. This way, you can avoid causing any damage! B/C Now, insert the 10x eyepiece (1) into the Barlow lens (3). Make sure that the Barlow lens is placed all the way into the eyepiece supports (4) and is not pulled out. How do I observe the specimen?
After you have assembled the microscope with the adequate illumination and adjusted it correctly , the following basic rules are to be observed: Start with a simple observation at the lowest magnification. This way, it is easier to position the object in the middle (centering) and make the image sharp (focusing). The higher the magnification, the more light you will require for a good image quality.

Now place the prepared specimen (17) directly under the objective on the microscope stage. The object should be located directly over the illumination (10). In the next step, take a look through the eyepiece (1) and carefully turn the focus knob (14) until the image appears clear and sharp. Now you can select a higher magnification by slowly removing the Barlow lens (3) from the eyepiece support (4). When the Barlow lens is almost completely pulled out, the magnification can be increased to almost double. If you would like an even higher level of magnification, insert the 20x eyepiece (2) and turn the objective nosepiece (6) to a higher setting (10x or 40x).

Important tip:

The highest magnification is not always the best for every specimen!

Note:
Each time the magnification changes (eyepiece or objective change, pulling out the Barlow lens), the image sharpness must be readjusted with the focus knob (14). When doing this, make sure to be careful. If you move the microscope stage too quickly, the objective and the slide could come into contact and become damaged!

Which light for which specimen?

With this unit, a reflected light and transmitted light microscope, you can observe transparent, semi-transparent as well as non-transparent objects. The image of the given object of observation is “transported” through the light. As a result, only the correct light will allow you to see something! If you are observing non-transparent (opaque) objects (e.g. small animals, plant components, stones, coins, etc.) with this microscope, the light falls on the object that is being observed. From there, the light is reflected back and passes through the objective and eyepiece (where it gets magnified) into the eye. This is reflected light microscopy. For transparent objections (e.g. protozoa), on the other hand, the light shines from below, through the opening in the microscope stage and then through the object. The light travels further through the objective and eyepiece, where it is also magnified, and finally goes into the eye. This is transmitted-light microscopy. Many microorganisms in water, many plan components and the smallest animal parts are already transparent in nature. Others have to be prepared. We may make them transparent through a treatment or penetration with the right materials (media), or by taking the thinnest slices from them (using our hand or a specimen slicer), and then examine them. You can read more about this in the following sections.

How do I make thin specimen slices?

Only do this with the supervision of your parents or another adult. As I already pointed out, the thinnest slices possible are taken from an object. In order to get the best results, we need some wax or paraffin. It is best if you get a candle. Place the wax in a pot and heat it carefully over a low burner. Now, dip the object in the liquid wax a few times. Then, let the wax get hard. Using the Specimen slicer (19) or a knife/scalpel, cut the smallest slices from the object that is covered with wax. These slices are to be laid on a slide and covered with a cover slip. How do I make my own specimens?
Take the object that you want to observe and place it on a glass slide (17). Then, add a few drops of distilled water on the object using a pipette. Now, place a cover slip vertically at the edge of the drop of water, so that the water runs along the edge of the cover slip. Then, slowly lower the cover slip over the water drops

Note :
The included glue “gum media” (18b) is used to make permanent prepared specimens. Use this in place of the distilled water. If you want to keep the object in place on the slide permanently, use the gum media.

Experiments

Now that you’re familiar with your microscope’s functions and how to prepare slides, you can complete the following experiments and observe the results under your microscope.

How do You Raise Brine Shrimp?

Accessories (from your microscope set):

  1.  Shrimp eggs
  2.  Sea salt,
  3.  Hatchery,
  4.  Yeast.

The Life Cycle of Brine Shrimp
Brine shrimp, or “Artemia salina,” as they are called by scientists, have an unusual and interesting life cycle. The eggs produced by the female are hatched without ever being fertilized by a male shrimp. The shrimp that hatch from these eggs are all females. In unusual circumstances, e.g. when the marsh dries up, the male shrimp can hatch. These males fertilize the eggs of the females and from this mating, special eggs come about. These eggs, so-called “winter eggs,” have a thick shell, which protects them. The winter eggs are very resistant and capable of survival if the marsh or lake dries out, killing off the entire shrimp population. They can persist for 5-10 years in a “sleep” status. The eggs hatch when the proper environmental conditions are reproduced. These are the type of eggs you have in your microscope set.

The Incubation of the Brine Shrimp
In order to incubate the shrimp, you first need to create a salt solution that corresponds to the living conditions of the shrimp. For this, put a half liter of rain or tap water in a container. Let the water sit for approx. 30 hours. Since the water evaporates over time, it is advisable to fill a second container with water and let it sit for 36 hours. After the water has sat stagnant for this period of time, add half of the included sea salt to the container and stir it until all of the salt is dissolved. Now, put a few eggs in the container and cover it with a dish. Place the glass container in a bright location, but don’t put it in direct sunlight. Since you have a hatchery, you cal also add the salt solution along with a few eggs to each of the four compartments of the tank. The temperature should be around 25º. At this temperature, the shrimps will hatch in about 2-3 days. If the water in the glass evaporates, add some water from the second container.

The Brine Shrimp under the Microscope

The animal that hatches from the egg is known by the name “nauplius larva.” With the help of a pipette, you can place a few of these larvae on a glass slide and observe them. The larvae will move around in the salt water by using their hair-like appendages. Take a few larvae from the container each day and observe them under the microscope. In case you’ve hatched the larvae in a hatchery, simply take off the cover of the tank and place the tank on the stage. Depending on the room temperature, the larvae will be mature in 6-10 weeks. Soon, you will have had raised a whole generation of brine shrimp, which will constantly grow in numbers.

Feeding your Brine Shrimp

In order to keep the brine shrimp alive, they must be fed from time to time, of course. This must be done carefully, since overfeeding can make the water become foul and poison our shrimp population. The feeding is done with dry yeast in powdered form. A little bit of this yeast every second day is enough. If the water in the compartments of the hatchery or your container turns dark, that is a sign that it is gone bad. Take the shrimp out of the water right away and place them in a fresh salt solution.

Textile fibers

Objects and accessories:

  • Threads of different textiles: Cotton, linen, wool, silk, Celanese, nylon and any others you can find.
  •  Two needles:
    • Put each thread on a glass slide and fray each with the help of the two needles. Put a drop of water over each thread with the pipette and cover each with a cover glass. Adjust the microscope to a low magnification. Cotton fibres are of plant origin and look, under the microscope, like a flat, twisted band. The fibres are thicker and rounder at the edges than in the centre. Cotton fibres consist primarily of long, collapsed tubes. Linen fibres are also of plant origin; they are round and run in straight lines. The fibres shine like silk and exhibit numerous swellings along the shaft of the fibre. Silk is of animal origin and consists of solid fibres of smaller diameter than the hollow vegetable fibres. Each silk fibre is smooth and even and has the appearance of a small glass rod. Wool fibres are also of animal origin; the surface consists of overlapping scales, which appear broken and wavy. If possible, compare wool fibres from different weaving mills, and note the differences in the appearance of the fibres. Experts can determine the country of origin of wool based on its appearance under a microscope. Celanese is artificially manufactured by a long chemical process. All Celanese fibres show hard, dark lines on a smooth, shining surface. The fibres crinkle in the same way after drying. Observe the similarities and differences between the different fibres.

Troubleshooting

Smartphone holder

Open the flexible holder and put your smartphone in it. Close the cradle and make sure your phone is properly seated. The camera must be positioned exactly above the eyepiece. Open the locking clip on the back of the holder and fit the eyepiece view exactly onto your smartphone camera. Now retighten the locking clip and attach the smartphone holder to the eyepiece of your microscope. Now start the camera app. If the image is not yet centered on your display, loosen the locking clip slightly and readjust. It may be necessary to use the zoom function to fill the image on the display. A slight shading at the edges is possible. Remove the smartphone from the cradle after use

NOTE:
Make sure that the smartphone cannot slip off the cradle. Bresser GmbH accepts no liability for damage caused by a dropped smartphone!

Make sure your microscope has a long service life.

Clean the lens (objective and eyepiece) only  with the cloth supplied or some other soft lintfree cloth (e.g.microfibre). Do not press hard as this might scratch the lens. Ask your parents to help if your microscope is really very dirty. The cleaning cloth should be moistened with cleaning fluid and the lens wiped clean using little pressure. Make sure your microscope is always protected against dust and dirt. After use leave it in a warm room to dry off. Then install the dust caps and keep it in the case provided.

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MICROSCOPE FAQ:

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MANUAL DOWNLOAD:

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EXPERIMENTS:

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Warranty & Service
The regular guarantee period is 5 years and begins on the day of purchase. You can consult the full guarantee terms and details of our services at www.bresser.de/warranty_terms

References

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