HANNA ISTR3844R2 Hydrogen Peroxide Test Kit Instruction Manual

: June 4, 2024
: HANNA

Table of Contents

ISTR3844R2 Hydrogen Peroxide Test Kit
SPECIFICATIONS
SIGNIFICANCE AND USE
CHEMICAL REACTION
INSTRUCTIONS
Documents / Resources

ISTR3844R2 Hydrogen Peroxide Test Kit
Instruction Manual

ISTR3844R2 Hydrogen Peroxide Test Kit

Dear Customer,
Thank you for choosing a Hanna Product. Please read the instructions carefully before using the chemical test kit. It will provide you with the necessary information for the correct use of the kit.
Remove the chemical test kit from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any noticeable damage, notify your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:

HI 3844A-0 Hydrogen Peroxide Reagent, 1 bottle (100 mL) ;
HI 3844B-0 Hydrogen Peroxide Reagent, 1 bottle (17g) ;
HI 3844C-0 Hydrogen Peroxide Reagent, 1 bottle with dropper (30 mL);
Starch Indicator, 1 bottle with dropper (30 mL)
1 plastic test tube, graduated with cap;
1 calibrated plastic vessel (50 mL);
1 plastic pipette (3 mL);
1 plastic pipette (1 mL), for HI 3844A-0 Reagent;
1 plastic spoon.

Note: Any damaged or defective item must be returned in its original packing materials.

SPECIFICATIONS

Range| 0 to 2 ppm H2O2 Low Range
0 to 10 ppm H2O2 High Range
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Smallest Increment| 0.25 ppm H2O2 Low Range
1.0 ppm H2O2 High Range
Analysis Method| Drop-Count Iodometric Titration
Sample Size| 25 mL Low Range
5 mL High Range
Number of Tests| 100 (average)
Case Dimensions| 235x175x115 mm (9.2×6.9×4.5″)
Shipping Weight| 450 g (15.9 oz.)

SIGNIFICANCE AND USE

Hydrogen peroxide is widely used as a disinfectant and as a bleach for textiles, wood pulp, hair, fur, etc. It is also used as a substitute for chlorine in water and sewage treatment.
Most common commercial forms are aqueous solutions containing about 6, 12, and 30 percent hydrogen peroxide and are referred to as “20-volume”, “40-volume” and “100volume” respectively, meaning the value of oxygen liberated when the solution is boiled. The Hanna test kit can quickly and easily determine the concentration in water up to 10 ppm of hydrogen peroxide. This is due to the fact that it is not affected by stabilizers, which are sometimes added to commercial hydrogen peroxide solutions. The kit is portable and can be used in the field as well as in the laboratory.
Note: mg/L is equivalent to ppm (parts per million).

CHEMICAL REACTION

Hydrogen peroxide is determined by a titrimetric method. It reacts slowly with iodide in an acid solution (Step 1); thus a 15 minutes interval is required to allow the reaction to occur completely. The amount of iodine generated is equivalent to the hydrogen peroxide in the sample. The liberated iodine is then titrated with a standard sodium thiosulfate solution that reduces the iodine back to iodide ions (Step 2).
Step 1: H2O2 + 2H+ + 2I– → I2 + 2H2O
Step 2: I2 + 2S O32– →2I– + S4O62

INSTRUCTIONS

READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT

Remove the cap from the plastic vessel and rinse it with the sample. For H2 O2 Low Range fill it up to the 25 mL mark with the sample. In the case of H2 O2 High Range fill the vessel with 5 mL of the sample using the graduated plastic test tube and add D.I. water to the 15 mL mark.
Add 1 mL of HI 3844A-0 reagent for Low Range or 0.5 mL for High Range using the plastic pipette and swirl gently to mix.
Using the plastic spoon provided, add 1 spoon of HI 3844B-0 reagent and swirl gently to mix.
Wait for 15 minutes to allow the reaction to occur, leaving the plastic vessel closed and in a dark place. Note: if hydrogen peroxide is present, the solution will turn to a dark yellow color.
Remove the cap. Slowly add drops of the titration reagent HI 3844C-0 while swirling the solution and counting the drops. Continue adding the titration solution until the yellow color of the solution is almost colorless.
Add 2 to 3 drops of Starch Indicator and mix by carefully swirling the plastic vessel in tight circles. The solution will turn a blue color.
Continue adding slowly drops of the titration reagent HI 3844C-0, while swirling and counting the drops, until the solution changes from blue to colorless.
To obtain the concentration in ppm of Hydrogen Peroxide in your sample, multiply the total number of drops of the titration reagent HI 3844C-0 used to turn the solution from the dark yellow color to colorless by 0.25 or 1 for Low Range or High Range, respectively.

of DROPS * 0.25 = ppm Hydrogen Peroxide LR

of DROPS * 1 = ppm Hydrogen Peroxide HR

Note: after a few minutes, the blue color of the starch/iodine complex may reappear after the titration has been completed due to air oxidation of the iodine.

REFERENCES
Vogel’s, Textbook of quantitative chemical analysis, 5thed., Longman Scientific and Technical
HEALTH AND SAFETY
The chemicals contained in this test kit may be hazardous if improperly handled. Read Health and Safety Data Sheets before performing the test.

www.hannainst.com
ISTR3844R2 05/21

Documents / Resources

| HANNA ISTR3844R2 Hydrogen Peroxide Test Kit [pdf] Instruction Manual
ISTR3844R2 Hydrogen Peroxide Test Kit, ISTR3844R2, Hydrogen Peroxide Test Kit, Peroxide Test Kit, Test Kit
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